RU2538051C2 - Method of treating latent mastitis in cows - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to agriculture, particularly to veterinary medicine, and aims at providing higher clinical effectiveness in latent mastitis in cows. That is ensured by using mosquito's salivary secretions as biologically active substances. To this effect, 20 laboratory mosquitoes are introduced on the skin of the affected udder lobes. The treatment is administered for 3 days every 24h at any time of day.

EFFECT: method provides the 100% clinical effectiveness in latent mastitis, being less labour-consuming and environmentally safe as compared to the other methods.

Description

The invention relates to agriculture, in particular to veterinary medicine, and in particular to a method for the treatment of latent (subclinical) mastitis in cows.

In modern livestock breeding, hidden (subclinical) mastitis is a major economic and economic problem, which occurs 10 times more often than clinically expressed mastitis. In a single study, 35-40% of patients with subclinical mastitis are detected, and up to 50-60% of cows are ill during the year, of which 40% are repeated.

The secret of affected udder lobes has low acidity, density, osmotic pressure, and the quality and nutritional properties of milk deteriorate. This changes the content of casein, whey proteins, fat, amino acids and other components of milk. Milk from cows with mastitis patients loses not only nutritional properties, but is also unsuitable for technological processing.

Mastitis has a negative effect on the reproductive function of cows, often causing diseases of the uterus, and also reduce ovarian function, which in turn leads to prolonged infertility of animals.

According to the US National Council on Mastitis, the total damage caused by this disease consists of a decrease in milk yield - 70%, premature culling of cows - 14%, deterioration in milk quality - 8%. In monetary terms, the damage is annually in the USA - 2 billion dollars, Germany - 60 million marks, Denmark - 200 million crowns, Great Britain - 110 million pounds, Russia - 1.5 billion rubles.

Currently, the means and methods of treating latent mastitis in cows do not provide a reliable therapeutic effect. In addition, most medications are synthetic and contain antibiotics and other chemotherapeutic substances to which the animal organism is not phylogenetically adapted, and this leads to various side effects - allergies, a decrease in the general and local natural resistance of the body. The unsystematic use of antibiotics leads to the emergence of the latest strains of microbes resistant to them. Combined milk containing antibiotics violates the technology in the manufacture of fermented milk products and their culling.

Currently, the following methods for treating hidden (subclinical) mastitis in cows are best known:

The complex preparation - Mastisan A, contains benzylpenicillin, streptomycin, norsulfazole or sulfadimezin, vegetable or liquid paraffin, emulsifiers.

When using Mastisan A, it must be heated to 36-39 ° C and shaken until a uniform suspension is obtained. 2-3 doses of the drug are injected into the affected lobe, depending on the severity of the process. If necessary, the introduction is repeated every 24 hours (1-s. 196).

The drug - Mastisan B, contains neomycin, sulfadimesin, methyluracil, diastaratpolyglycerol, petrolatum in its composition.

Before use, Mastisan B is heated to 37 ° C and injected into the affected lobe through the teat canal in a dose of 5 ml twice a day (after milking). The course of treatment is 2-3 days (1-p. 196).

The drug - Mastisan E - a suspension of erythromycin and sulfadimezin in a polyethylsiloxane liquid with an emulsifier.

Enter into the affected quarter warmed up with an interval of 12 hours 2-3 times in a dose of 5-15 ml, if necessary, repeat the treatment (1-p.196).

Masticide - a suspension of penicillin, streptomycin, sulfadimizine and norsulfazole in vegetable oil.

Before use, the drug is heated to 39 ° C and shaken thoroughly. Then, it is administered to lactating cows intramuscularly after milking twice a day, every 12 hours, and to dry cows after 12-24 hours. The course of treatment is carried out for 2-3 days (1-p. 196).

Masticide-2 is a complex preparation containing 200 thousand units in 10 ml of benzathinepenicillin, streptomycin sulfate - 100 thousand units, sulfadimesin - 0.35 g, vegetable oil - 10 ml, beeswax or aluminum stearic acid - 0.4 g.

Masticide-2 is introduced into the affected quarter of the udder after evening milking once in a dose of 10 ml. After 10 days, the drug is re-administered if necessary (1-p. 197).

Difurol A is a preparation containing furagin, furazolidone, polyethylene oxide-400 and dimexide. The drug is injected through the nipple canal into the affected quarter warmed up to 36-39 ° C. The dose of difurol is 10-20 ml with an interval of 12-24 hours. The course of treatment is 3-4 days. If necessary, the treatment is repeated after 10-15 days (1-s. 198).

According to some authors, the effectiveness of these drugs used for hidden mastitis in cows ranges from 50 to 65%. However, all these drugs include antibiotics and chemotherapeutic substances, which have, as indicated above, a negative effect on the animal organism. In addition, the introduction of these drugs is time-consuming, since they should be administered directly into the affected quarter of the mammary gland, necessarily after milking and for several days.

The purpose of the invention is the achievement of high therapeutic efficacy with hidden mastitis in cows in a less laborious and environmentally friendly way.

Our proposed method for treating latent mastitis in cows with the help of laboratory mosquitoes (mosquito therapy) does not have side effects, and most importantly - environmentally friendly. This is primarily due to the fact that in the process of phylogenesis they adapted to coexistence with mosquitoes in one or another biogeocenosis. In addition, this method is less time consuming and does not require the introduction into the affected quarter of drugs, which in turn violate the integrity of the glandular tissue.

However, laboratory mosquitoes are currently bred artificially in entomosis laboratories and can be marketed. This makes it possible to obtain environmentally friendly laboratory mosquitoes, as well as free from various pathogens of infectious and invasive diseases.

Currently, mosquitoes are not used by humans or animals.

The mechanism of action of mosquito therapy is complex and diverse, but so far it has not been fully studied. Currently, mosquito therapy is based on the use of a biologically active substance in the salivary secret of mosquitoes.

The therapeutic effect of mosquitoes is determined by the properties of the secretion of the salivary glands, which enters the body of animals and humans after piercing the skin and continues to flow throughout the entire period of bloodsucking. The secret has anticoagulant, resolving thrombi action, prevents their formation. The biologically active substances produced by the mosquito have the ability to relieve vasospasm, increasing the supply of tissues with oxygen and other nutrients, expanding them, lowering blood pressure, has analgesic effects. An important mechanism of comarotherapy is its reflex effect on the body, both general and local. This mechanism takes effect from the moment of suction, i.e. from the beginning of the action of biologically active substances. The pulses in the zones are transmitted to certain segments of the spinal cord, reflexively changing the functions of the autonomic and central nervous system. At the same time, impulses from the excited section are continuously sent to the central nervous system and, summing up, mobilize the body's forces to combat the emerging threat to it.

It is also important that the biologically active substances produced by the mosquito activate the body's immune system. Pathogenic microorganisms in the blood (Staphylococcus aureus, Escherichia coli, etc.) are not only suppressed, but also destroyed by leukocytes due to the restoration or increase of their ability to capture and digest microbes and the remains of destroyed blood cells.

To date, it has been established that in addition to anticoagulants, a number of other biologically active compounds are contained in the secretion of the salivary glands of mosquitoes.

Considering the fact that until now, comarotherapy has not found proper attention in the arsenal of a veterinarian in the treatment of various diseases in animals, including latent mastitis, we filled this gap.

13 cows were selected for the experiment on the principle of analogues, patients with a latent form of mastitis, one share of the udder was affected in each animal.

The diagnosis of latent mastitis in cows was established on the basis of the absence of clinically pronounced signs of inflammation of the udder and positive reactions of milk with a 2% solution of mastidine. In order to exclude irritation of the udder, milk studies were repeated after 3 days.

When confirming the diagnosis of latent mastitis, the affected portion of the udder was irrigated, and then a cage with mosquitoes was brought to the affected portion. 20 mosquitoes were used per treatment session. After blood saturation (4-5 minutes), mosquitoes spontaneously flew away.

The course of treatment was repeated three times with an interval of 24 hours

7 days after the end of the course of treatment, milk was again examined using a 2% mastidine solution to determine the therapeutic effectiveness of mosquito therapy with latent mastitis.

The results of the studies showed that during komaroterapii 13 cows, in which 15 shares of the udder was affected by hidden mastitis, all recovered. Thus, the effectiveness of comarotherapy with hidden mastitis was 100%, which is significantly higher than with antibiotic-containing and chemotherapeutic drugs.

Information sources

1. Mutovin V.I. Fighting mastitis of cows. M .: Kolos, 1974.

2. Kamenev Yu.Ya., Kamenev O.Yu. A leech will help you. Hirudotherapy. Set, St. Petersburg - 1997.

Claims (1)

A method of treating latent mastitis in cows by introducing biologically active substances into the affected portion of the udder, characterized in that the secretion of the salivary glands of mosquitoes is used as biologically active substances, and the introduction is carried out for 3 days with an interval of 24 hours at any time by planting 20 laboratory mosquitoes that spontaneously fly away after 4-5 minutes on the skin of the affected lobe of the udder.