RU2678082C1 - Method for treating pigs in case of dysentery and other bacterial diseases - Google Patents

Abstract

FIELD: veterinary.SUBSTANCE: invention relates to veterinary medicine and is intended for treatment of pig dysentery. Combination of antibacterial preparations in the form of emulsion on oil solution of eleovite is used with the following ratio of components, mass %: trichopol (metronidazole) – 2; tylan – 14; tiamulin – 5; eleovit – 32; distilled sterile water – 47. Drug is injected into muscles at a dose of 1 ml per 10 kg of body weight of the animal, but no more than 5 ml per injection, 3 times at intervals of 48 hours.EFFECT: method provides higher treatment effectiveness.1 cl, 4 tbl

Description

FIELD OF THE INVENTION

The invention relates to agriculture, to veterinary medicine, namely, to methods for treating dysentery and other bacterial diseases of pigs.

State of the art

For the treatment of pig dysentery (pathogen Brachiospira hyodysenteriae), many methods have been proposed using antibiotics, nitrofurans, sulfonamides [1].

A known method of treating pig dysentery with the combined use of tylosin (tilan): in the form of injections into the muscles of 8-10 mg per 1 kg of body weight and at the same time feeding tylosin 100 g per 1 ton of feed. The course of treatment is 5 days [2]. The disadvantages of this method are the complexity and cost.

A known method of treating pig dysentery using metronidazole (Trichopolum) in the form of injections in liquid paraffin at a dose of 10-15 mg per 1 kg of body weight [3].

There is a method of treating dysentery of pigs with Trichopolum when feeding it with food at a dose of 250 g per 1 ton of feed, 2 times a day, 3-5 days. However, with this method it is not possible to create bactericidal concentrations of the active substance against brachiospir in the body of sick animals, moreover, this action cannot be prolonged [4].

Previously, we proposed a method for the treatment of pig dysentery (Patent No. 2442573 for application No. 201031289), including parenteral administration of antibacterial substances, distilled water, and trivitamine [6].

The combination of antimicrobial drugs Trichopolum and Tilan used in this method is effective against pathogens of pig dysentery, against leptospirosis of animals. However, in recent years, the resistance of pathogens to antibacterial agents has increased. The need for antibacterial agents, effective against both gram-negative and gram-positive bacteria, inhibiting the growth and development of both anaerobic microflora and pathogens of an aerobic nature has also increased.

One of the tested methods for increasing the effectiveness of antibiotics, preventing or slowing the formation of the resistance of pathogens to the action of these drugs is combined antibiotic therapy [7].

The purpose of the invention

The creation of a new method for the treatment of dysentery and other bacterial diseases of pigs, the use of which provides high therapeutic efficacy and does not cause inflammatory reactions with parenteral administration of the drug.

In order to enhance antibacterial activity and expand the antimicrobial spectrum, the claimed method proposes a preparation, the use of which provides high therapeutic efficacy in parenteral administration, primarily in pig dysentery, as well as other bacterial diseases.

This goal is achieved by the fact that, in addition to tilan and trichopolum, tiamulin in quantities sufficient to ensure a high therapeutic effect is also introduced into the composition of the drug as active ingredients. For the treatment of pigs with dysentery and other bacterial diseases, a drug (emulsion) is proposed, including (wt.%):

Trichopolum (metronidazole)  2 Tilan  fourteen Tiamulin  5 Eleovit  32 Sterile distilled water  47

Distinctive features of the drug used in the proposed method of treatment and the known drug described in patent No. 2442573 are presented in table 1.

Due to the affiliation of the antimicrobial agents included in the composition of the proposed for the implementation of the new method, to different chemotherapeutic groups, they have different spectra of antimicrobial activity. Table 2 provides information on the spectra of sensitive bacteria to drugs.

The data shown in table 2 show that the drugs have different targets when exposed to microbes on the body, which determines the synergistic effect of this composition. A different spectrum of sensitive organisms allows you to use the method to combat not only dysentery, but also with other bacterial diseases of pigs, for example, with enzootic pneumonia of pigs.

An example of the manufacture of the proposed drug

The drug is prepared before use as follows:

140 g of tilane are added to a sterile container with a volume of 2-3 liters and dissolved in 470 ml of sterile distilled water, 20 g of trichopolum and 50 g of tiamulin are added to the solution of tilan, the components are dissolved with stirring. Then the solution is centrifuged at 3000 rpm for 10 minutes. The supernatant is drained, the precipitate is removed from possible mechanical impurities. The resulting solution was poured into 68 ml into sterile vials and 32 ml of eleovit was added. The vials are closed with sterile rubber stoppers and sealed with aluminum caps.

Before use, the drug is heated to animal body temperature and shaken. For the treatment of pigs with dysentery and other bacterial diseases, the drug emulsion is administered intramuscularly (young animals in the thigh muscles, adults in the muscles of the scapula, neck) at a dose of 1 ml per 10 kg of body weight, but not more than 5 ml per 1 time. The drug is administered three times at intervals of 48 hours. The entire course of treatment is 6 days.

An example of a real use of the claimed method

In the farm where the disease of pigs with dysentery was established (the causative agent of Brachiospira hyodysenteriae) (Act No. 1), a treatment method using the claimed preparation of the above composition (wt.%) Was tested:

Trichopolum (metronidazole)  2 Tilan  fourteen Tiamulin  5 Eleovit  32 Sterile distilled water  47

The emulsion of the drug was administered intramuscularly at a dose of 1 ml per 10 kg of body weight, but not more than 5 ml at a time. The drug was administered three times with intervals between injections for 48 hours. At a low temperature, the drug was heated to body temperature and shaken before administration. The claimed method was processed 250 pigs.

The control was sick pigs, for the treatment of which they used: control group No. 2 - the method and preparation of the patent No. 2442573; control group No. 3 - Tim Til-250 at a dose of 0.8 ml per 10 kg of animal weight.

On the farm where the disease of pigs of mixed etiology was found: dysentery (the causative agent of Brachiospira hyodysenteriae) and enzootic pneumonia (the causative agent of Mycoplasma hyopneumoniae) (Act No. 2, Conditional name of the TTTE preparation, is used only in Act No. 2), a treatment method using the declared the preparation of the above composition (wt.%):

Trichopolum (metronidazole)  2 Tilan  fourteen Tiamulin  5 Eleovit  32 Sterile distilled water  47

The emulsion of the drug was administered intramuscularly at a dose of 1 ml per 10 kg of body weight, but not more than 5 ml at a time. The drug was administered three times with intervals between injections for 48 hours. At a low temperature, the drug was heated to body temperature and shaken before administration. The claimed method was processed 2500 pigs. The control was sick pigs, for the treatment of which they used the antibacterial drug Dizparkol, which was injected into the muscles at a dose of 0.15 ml per 1 kg of animal weight, not more than 5 ml per 1 injection.

The data shown in tables 3 and 4 indicate a high therapeutic efficacy of the claimed method.

Technical result

The technical result, objectively manifested in the implementation of the proposed method is as follows:

1. An increase in the safety of the livestock of pigs to 100% (against 96-98%) with dysentery, as well as up to 100% (against 78%) with mixed infection.

2. Improving the effectiveness of veterinary measures for pig dysentery.

3. Introduction to the composition of the drug used in the implementation of the claimed method, tiamulin increased antibacterial activity and expanded the spectrum of antibacterial activity of the drug.

4. Introduction to the composition of the drug eleovit instead of trivitamine allows you to maintain the prolonging effect of the drug and provides higher therapeutic efficacy due to the presence of a wide range of vitamins in the composition of eleovit (10 vs. 3).

5. Due to the absence of irritating effect, the advantage of parenteral administration of the drug is more fully manifested - the rapid accumulation of active substances in bactericidal concentrations against Brachiospira hyodysenteria and Mycoplasma hyopneumoniae.

Bibliography

1. Skvortsov V.N. and other pig dysentery. - Guidelines. - M., 2009.

2. Antipov V.A. The use of tylosin in veterinary medicine and livestock. - J. “Agriculture Abroad,” No. 6, 1984, p. 43-46.

3. Tikhonov V.L., Skvortsov V.N. The use of metronidazole in pig dysentery. - News is a breeze. farm. honey. - 1992. - T. 8, No. 2. - from. 48-50.

4. Sidorkin V.A., Gavrish V.G., Egunova A.V., Ubiraev S.P. Diseases of pigs / Under the total. ed. V.A. Sidorkina. - M .: Aquarium-Print LLC, 2007, p. 322-327.

5. Soldatenko N.A., Azaryan R.P., Polubedov S.G., Radkov V.N. "A method for the treatment of pigs - leptospiracetes." - AS No. 1513663, 06/08/1989

6. Soldatenko N.A., Fetisov L.N., Streltsov N.V. "A method for the treatment of pig dysentery." - Patent No. 2442573, publ. 02/20/2012, bull. No. 5.

7. Navashin S.M., Fomina I.P. Rational antibiotic therapy / Reference book. M., "Medicine", 1982. - S. 335-344.

Claims (3)

A method of treating pig dysentery, including parenteral administration of antibacterial substances, distilled water and eleovit, characterized in that in order to enhance antibacterial activity, expand the antibacterial spectrum and increase therapeutic efficacy, the composition of the drug is used in the following ratio of components, mass. %: Trichopolum (metronidazole)  2 Tilan  fourteen Tiamulin  5 Eleovit  32 Sterile distilled water  47 moreover, the drug is administered at a rate of 1 ml per 10 kg of animal body weight, but not more than 5 ml per 1 injection, three times at intervals of 48 hours.