RU2613318C1 - Method for treating postpartum endometritis in milch cows - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to field of veterinary and is intended for treatment of postpartum endometritis. Biopag-D is introduced into cow's uterus in form of 0.01-0.02% water solution two-three times with 2 day (48 h) interval in dose 100-150 ml per one introduction per one cow. Medication is introduced by means of Janet syringe with polystyrene pipette for artificial insemination. Solution is introduced heated to temperature 38-40°C.

EFFECT: invention makes it possible to treat postpartum endometritis in milch cows.

3 cl, 16 tbl, 2 ex

Description

The invention relates to the field of veterinary medicine, in particular to methods and means of treating postpartum obstetric and gynecological diseases in dairy cows, and can be used both in large livestock complexes, and in farm and personal farms.

A known method of treating endometritis in animals with the antibacterial drug "Dorin" based on antibiotics of a wide spectrum of action of rifampicin and doxycycline. The drug dissolved in water is administered intrauterine with a Janet syringe once a day at a rate of 0.2-0.4 mg per kg of animal body weight 1-2 times (see RF Patent No. 2142804, A61K 31/65. Publ. 12/20/99. Bull. No. 35).

The disadvantage of this method is that the aqueous solution of antibiotics does not penetrate deeply into the tissue of the animal, which reduces its effectiveness, and when sucked, it is excreted in milk, which, in turn, leads to culling of milk and economic damage to the farm.

There is also a method of treating and preventing endometritis in cows using enrofloxacin and dimethyl sulfoxide enrocide in distilled water, which is administered intrauterine no more than 5 times using a Janet syringe in a dose of 100 ml with an interval of 48 hours in combination with other drugs (see the Instruction on the use of the drug enrocid CJSC NLP Agrofarm, Voronezh, 2003 No. PVR-2-0.301215).

The disadvantage of this method is that the mono-composition of the drug somewhat limits the possibility of its use, since microorganisms develop resistance to any antibiotic over time. Dimethyl sulfoxide increases the absorption of the drug, while increasing its toxicity and allergization of the animal organism, in addition, a strong and unpleasant odor can cause allergic reactions in the staff.

There is also known a tool based on a water-soluble organosilicon derivative of glycerol (1: 1 aqueous solution), in which diseased cows are intrauterine injected 3-5 times at intervals of 48 hours at a rate of 50-100 g per administration with a Janet syringe and a polystyrene pipette for artificial insemination, in this case, before administration, the product is diluted with sterile distilled water in a ratio of 1: 1 (see patent No. 2404756. "Means and method of treating endometritis in cows." Publish. November 27, 2010. Bull. No. 33).

The disadvantage of this method is that it contains antibiotics, which, penetrating into the tissue with the help of a water-soluble organosilicon derivative of glycerol, can penetrate into the blood and then excreted with milk, which leads to its culling and material losses in the economy.

The objective of the invention is the selection of a broad-spectrum antimicrobial agent for intrauterine administration and a method for its use for the treatment of postpartum chronic endometritis in dairy cows, not causing the development of resistance of microorganisms, odorless and not causing allergic reactions in animals and maintenance personnel, but with increased adhesion to animal tissues, which provides a prolonged therapeutic effect in complex therapy with significant treatment effectiveness and low costs preparing a solution and its use. In addition, the drug should not be excreted with milk, preventing the loss of milk from culling and economic damage on the farm.

The problem is solved by the fact that, as a therapeutic drug, the Biopag-D antimicrobial agent is proposed and clinically tested in laboratory and industrial conditions, containing a synthetic drug, including a polymer - polyhexamethylene guanidine hydrochloride in a 0.01-0.02% concentration on an aqueous basis, and the introduction of the drug spend 2-3 times with an interval of 2 days (48 hours), at the rate of 100-150 ml per administration per cow, while the drug is administered to the cows in the uterus using a Janet syringe and a polystyrene pipette for artificial axis cows weighing up to 38-40 ° C, moreover, cows weighing up to 500 kg are injected with Biopag-D solution in an amount of 100 ml, and cows weighing more than 550 kg - 150 ml, while cows are prescribed magestrofan as a uterotonic agent in a dose of 2 ml per head 1 or 2 times with an interval of 48 hours.

Polymeric bactericides of the polyguanidine class, which belong to the group of disinfectants, have the ability to give the treated surfaces a bactericidal effect for a long time, capable of coating it with a “nanofilm” that is harmful to microorganisms but safe for humans and animals. Such a “nanofilm” is colorless and odorless, non-volatile (the polymer cannot get into the respiratory system and into the eyes of humans and animals), does not spoil processed equipment, does not decompose and does not emit toxic substances; the polymer itself is classified as a low-hazard substance in terms of toxicological properties, fire- and explosion-proof.

According to laboratory tests of the Research Institute of Disinfectology of Rospotrebnadzor, a single treatment with Biopag-D is detrimental to tuberculosis, staphylococcus and other dangerous microorganisms, (see Toxicological conclusion No. 504.1619.P.07 of 07.23.07; Act of acceptance technical tests No. ГЦ 334/2007 dated October 22, 2007 FGU Institute of Surgery named after A.V. Vishnevsky of the Russian Medical Technologies).

After laboratory research, a practical verification of the proposed method was carried out at the farms of the Sverdlovsk region.

Example 1

The studies were carried out in the SEC “Mezenskoye” in the Beloyarsky district of the Sverdlovsk region on cows of a dairy herd of Holstein breed with an average annual milk yield of 6 thousand kg of milk. For the study, 20 heads of cows with chronic purulent endometritis from the dairy herd were selected, the animals were divided into 2 groups according to the principle of analogues of 10 goals in each. Experimental group 1 and control group 2.

Sowing of vaginal secretions on conditionally pathogenic microflora and antibiotic sensitivity of the isolated microorganisms was carried out in the regional veterinary laboratory of Yekaterinburg. Within 2 weeks, a clinical assessment of the condition of the cows was carried out. A study of bacterial contamination of the vaginal mucus revealed enteropathogenic Escherichia coli and Proteus (Table 1).

Using serial dilutions, it was found that isolated strains of pathogenic microflora from vaginal mucus from cows are sensitive to Biopag-D at a concentration of 0.01%, and conditionally pathogenic - sensitive to Biopag-D at a concentration of 0.02% and 0.03%. The following treatment regimens were applied at the farm: using Biopag-D and a farm treatment regimen.

All animals from the experimental and control groups were determined biochemical and hematological status.

Blood was taken from animals before treatment and 30 days after the start of the experiment. Biochemical and hematological studies were carried out according to generally accepted methods (Table 2, 3, 4, 5).

The condition of animals with severe signs of impaired protein, fat and mineral metabolism. Cows show signs of immunodeficiency (lymphocytopenia) and erythrocytosis with a low level of hemoglobin. An increase in the number of monocytes characterizes the presence of purulent processes, in particular endometritis.

For the experimental group (10 goals) used the scheme:

1. In the postpartum period, from the 15th day after the birth (after transfer to the dairy herd), the intrauterine solution of Biopag-D 0.02% concentration at the rate of 100-150 ml per head, after 24 hours, intramuscularly, magestrofan in a dose of 2 ml - 10 goals as a drug with an uterotonic effect for uterine contraction and removal of contents).

For the control group, the following scheme was used:

2. In the postpartum period, the day after delivery, intramuscularly - gentamicin 4% solution of 19.0 ml 2 times a day for 5-7 days, then after transfer to the milking herd - warm sapropel rectally - 10 goals.

For animals of the experimental group, transferred to the milking herd, Biopag-D 0.01-0.02% concentration was administered intrauterine at a rate of 100-150 ml per head (depending on body weight up to 500 kg - 100 ml, and over 500 kg - 150 ml) two to three times.

Animals of the control group were treated with antibiotics used on the farm, and after inoculation for antibiotic sensitivity, gentamicin (since the absolute sensitivity of all microorganisms detected in the farm was revealed to it) intramuscularly for 5-7 days in the maternity ward. After being transferred to the dairy herd, sapropel was administered rectally for 7 days (Table 6). On the farm, in order to accelerate the involutional processes in the uterus after childbirth, sapropel is used rectally as a physiotherapy when transferring from the maternity ward to the milking herd.

Over the 6 months of the study, the antibiotic sensitivity of pathogenic microorganisms to antibiotics changed, namely: they became less sensitive to those antibiotics that were previously recommended (gentamicin) and the list of antibiotics choice was sharply reduced. In connection with a decrease in sensitivity to gentamicin, there is a need to search for new, more effective and expensive antibiotics. Sensitivity to Biopag-D remained virtually unchanged. Table 7.

The use of Biopag-D for the treatment of postpartum pathology can be used at a concentration of 0.02% intrauterine, combined with magestrofan (which has an uterotonic effect that increases uterine contractility) if more than 7 days have passed since calving. As an urotonic agent in the early post-hotel period, oxytocin can be used. The treatment efficiency was 70% (7 out of 10 cows became pregnant. Table 8).

Example 2

The studies were carried out on the basis of the Glinskoye GSP Rezhevsky district of the Sverdlovsk region. For the study, 22 cows were selected from the dairy herd of Holstein breed with an average annual milk yield of 5-6 thousand kg of milk. Cows were divided into two groups according to the principle of analogues, the average age in group 1 was experimental - 5.5, and 2 - control - 6.0 years (Table 9).

Diagnosis in dairy cows of both groups was carried out comprehensively taking into account anamnestic data, a general clinical study of cows with the establishment of characteristic clinical signs.

In animals, the clinical status was determined during the spring medical examination at the farm. In a clinical study, the state of all body systems was determined. On the farm, in order to accelerate the involutional processes in the uterus, after delivery, rectal sapropel is used as a physiotherapy when transferring from the maternity ward to the milking herd.

For animals of the experimental group, a Biopag-D solution of 0.01-0.02% concentration was calculated from the dairy herd at the rate of 100-150 ml per head (depending on body weight up to 500 kg - 100 ml, and over 500 kg - 150 ml ) two to three times. Animals of the control group were treated with antibiotics used on the farm, and after inoculation for antibiotic sensitivity, gentamicin (since the absolute sensitivity of all microorganisms detected in the farm was revealed to it) intramuscularly for 5-7 days in the maternity ward.

Using serial dilutions, it was found that in this farm the isolated strains of pathogenic microflora from the vaginal mucus from diseased cows are sensitive to Biopag-D at a concentration of 0.01%, and conditionally pathogenic are sensitive to Biopag-D at a concentration of 0.02% and 0, 03% (Table 10).

Blood was taken from animals before the start of the drug and 30 days after the start of the experiment. Biochemical and hematological studies were carried out according to generally accepted methods (Tables 11, 12). Sowing of vaginal swabs on conditionally pathogenic microflora and antibiotic sensitivity of the isolated microorganisms was carried out in the regional veterinary laboratory. Within 2 weeks, a clinical assessment of the condition of the cows was carried out. On the farm, 2 groups were selected:

Group 1 - experimental (12 goals) Biopag-D 0.02% solution, after 24 hours intramuscularly Magestrofan (with uterotonic properties) in a dose of 2 ml.

2 control - (10 goals) according to the treatment regimen of the farm, one day after calving, intramuscularly gentamicin 4% at a dose of 19 ml 2 times a day for 5-7 days (control of uterine bleeding), from day 7, rectally 100-150 g sapropel (to enhance involutional processes in the uterus) for 7-10 days.

Cows show signs of immunodeficiency (lymphocytopenia) and erythrocytosis with a low level of hemoglobin. An increase in the number of monocytes characterizes the presence of purulent processes, in particular endometritis.

After the studies, the effectiveness of treatment in the experimental group was 67% (table 15).

The use of Biopag-D solution with magestrofan increases the effectiveness of treatment to 67% compared with the basic treatment regimen on the farm - 50%, and the possibility of choosing antibiotics is limited in time (7 days after calving), and the identified microorganism strains develop antibiotic resistance over time (Table 16).

The use of Biopag-D for the treatment of chronic and acute postpartum endometritis is recommended to be used intrauterine at a concentration of 0.01-0.02%, combined with magestrofan or other urotonic agents, if endometritis lasts more than 7 days after delivery to improve uterine contractions and active excretion of its contents .

Claims (3)

1. A method of treating postpartum endometritis in dairy cows, comprising introducing into the uterus solutions of a medicinal product, characterized in that Biopag-D is used as a therapeutic drug, whereby Biopag-D is administered intrauterine two to three times with an interval of 2 days (48 hours) a volume of 100-150 ml, and Biopag-D is used in a concentration of 0.01-0.02% aqueous solution using a Janet syringe with a polystyrene pipette for artificial insemination, while the solution is injected in a heated form to a temperature of 38-40 ° C . 2. A method for the treatment of postpartum endometritis in dairy cows, comprising administering the uterine drug solutions according to claim 1. characterized in that 100 ml of Biopag-D solution is administered to cows weighing up to 500 kg, and 150 ml to cows weighing more than 550 kg , while the cows are prescribed magestrofan as an uterotonic agent in a dose of 2 ml per head 1 or 2 times with an interval of 48 hours 3. A method of treating postpartum endometritis in dairy cows, comprising introducing into the uterus the solutions of the drug according to claim 1. characterized in that in the early postnatal period, oxytocin, which stimulates lactation, is used as an uterotonic agent.