RU2323718C2 - Medicine to treat young stock gastrointestinal diseases - Google Patents

Abstract

FIELD: agriculture, veterinary science.

SUBSTANCE: medicine contains norfloxacin, dioxidin, 1.2-propylene glycol, low-molecular polyvinylpyrrolidone and distilled water in the following ratio (mass concentration %): norfloxacin - 9.0; dioxidin - 1.0; 1.2- propylene glycol- 19.8; low-molecular polyvinylpyrrolidone- 0.2; distilled water - the rest.

EFFECT: has an evident antimicrobial and therapeutic action.

11 tbl, 7 ex

Description

The invention relates to the field of veterinary medicine.

At the present stage of development of animal husbandry, respiratory and gastrointestinal diseases of calves and piglets are particularly widespread, due to the acquisition of collective farms from supply farms with different epizootic situations and non-compliance with standard indicators of their maintenance and feeding.

The union of calves, piglets with different immune status in the period of completing the livestock leads to their rebooting and an outbreak of infection.

The concentration in a limited area of a large number of monotonous, coeval, sensitive young animals causes the disease to quickly reach large groups of animals with a complex etiology and unclear symptom complexes.

The high incidence of young animals leads to a significant forced slaughter and mortality of animals, lack of live weight, which impedes the further development of animal husbandry.

The huge economic damage caused to livestock farms by gastrointestinal diseases necessitates the search for ways and methods to improve and find new effective means in their prevention and treatment.

In the complex of measures to combat gastrointestinal diseases of bacterial etiology in young farm animals, such chemotherapeutic agents as sulfonamides, antibiotics, nitrofurans, etc. are widely used. However, their prolonged and unsystematic use in animal husbandry has led to the appearance of resistant strains of microorganisms to them.

Known analogue - the drug "diarin" for the treatment and prevention of gastrointestinal diseases of young animals, containing, wt.%: Dioxidine 0.6-0.9, 40% lactic acid 10.0-15.0, methyluracil 0, 6-0.9, boiled water the rest [1].

Known analogue is a tool for the treatment of gastrointestinal diseases of young farm animals "diacetin" [2], containing an antibacterial agent dioxidine, an anti-inflammatory and antipyretic agent - acetylsalicylic acid, an immunomodulating agent thymalin and natural zeolites in the following ratio, wt.%:

Dioxidine - 2.5-5.0;

Acetylsalicylic acid - 25.0-75.0;

Timalin - 0.5-0.75;

Zeolites - The rest is up to 100.

The purpose of the invention is to increase the effectiveness of the treatment of gastrointestinal diseases of young farm animals.

This goal is achieved by using the drug consisting of the following components wt.%:

Norfloxacin - 9.0;

Dioxidine - 1.0;

1.2 Propylene glycol - 19.8;

Polyvinylpyrrolidone low molecular weight - 0.2;

Distilled water - the rest.

Norfloxacin - (Formula C ₁₆ H ₁₈ FN ₃ O ₃ ) is a drug that is similar in structure to pefloxacin. It differs from pefloxacin in the absence of a methyl group in the piperazinyl nucleus. It is a metabolite of pefloxacin, with the use of which a significant amount of norfloxacin is excreted in the urine [3].

Dioxidine - 1,4-di-N-oxide 2,3bio (oxymethyl) quinoxaline is a chemotherapeutic drug characterized by activity in infections caused by gram-positive and gram-negative bacteria [5, 6]. Dioxidine is effective against gram-negative, gram-positive pathogenic and conditionally pathogenic aerobic and anaerobic microorganisms of spore-forming and non-spore-forming species. The drug is active against microorganisms with low sensitivity and resistance to antibiotics and other chemotherapeutic agents.

1,2 Propylene glycol is the chemical formula of CH ₃ CHOHCH ₂ OH. It is a transparent, colorless, odorless, viscous substance with a sweet taste; soluble in water, alcohol and chloroform [7, 8].

Low molecular weight polyvinylpyrrolidone - chemical formula

It is a white powder. It is very easily soluble in water and mineral acids, soluble in alcohol, glycerin, acetone, dimethylformamide and products of chlorination of hydrocarbons, insoluble in ether and aliphatic hydrocarbons [9].

The drug is made as follows. The components are sequentially dissolved by heating no higher than 60 ° C.

The drug is administered at a dose of 0.5 ml / 10 kg of animal body weight 1 time per day. With colibacteriosis of calves and piglets, the drug is administered for 3 days. With salmonellosis of calves and piglets, the drug is administered for 5 days.

The essence of the method is illustrated by examples.

Example 1. The study of the optimal ratio of norfloxacin and dioxidine in a preparation for the treatment of gastrointestinal diseases of young farm animals in vitro experiments

The optimal ratio of norfloxacin and dioxidine in the preparation for the treatment of gastrointestinal diseases of young farm animals was studied in vitro by the method of serial dilutions in meat and peptone broth (MPB) with the determination of the minimum bacteriostatic concentration (MBsK). Comparison of the antimicrobial activity of norfloxacin and dioxidine and combinations of norfloxacin and dioxidine in a preparation for treating gastrointestinal diseases of young farm animals in various ratios. Conducted in relation to salmonella - Salmonella cholerae suis, E. coli - E. coli K88 (p), typified by morphological, biochemical, cultural and serological properties.

It was established (Table 1) that the combination of the formulation of the drug with the following ratio of components, wt.%: Has the most pronounced antimicrobial effect in relation to the studied strains of microorganisms.

Norfloxacin - 9.0;

Dioxidine - 1.0;

1.2 Propylene glycol - 19.8;

Polyvinylpyrrolidone low molecular weight - 0.2;

Distilled water - the rest.

Table 1 Antimicrobial activity of combinations of norfloxacin and dioxidine. Type of microorganisms Minimum bacteriostatic concentration, (MBsK), mcg / ml one 2 3 four 5 Salmonella cholerae suis 0.06 7.8 0.06 0,03 0.06 E.coli K88 (p) 0.24 31,2 0.06 0.06 0.12 Designations in the table:
1 - norfloxacin
2 - dioxidine
3 - Recipe 1 drug for the treatment of gastrointestinal diseases of young farm animals with a ratio of components: Norfloxacin - 9.0; Dioxidine - 0.5; 1.2 Propylene glycol - 19.8; Polyvinylpyrrolidone low molecular weight - 0.2; Distilled water - the rest.
4 - Formulation 2 of the drug for the treatment of gastrointestinal diseases of young farm animals with a ratio of components: Norfloxacin - 9.0; Dioxidine - 1; 1.2 Propylene glycol - 19.8; Polyvinylpyrrolidone low molecular weight - 0.2; Distilled water - the rest.
5 - Formulation 3 of the drug for the treatment of gastrointestinal diseases of young farm animals with a ratio of components: Norfloxacin - 8; Dioxidine - 1; 1.2 Propylene glycol - 19.8; Polyvinylpyrrolidone low molecular weight - 0.2; Distilled water - the rest.

MBSK of the combination according to formulation 2 with respect to Salmonella cholerae suis 0.03 μg / ml, and with respect to E. coli K88 (p) 0.06 μg / ml was superior to MBSK of both the constituent parts of the combination of norfloxacin and dioxidine, and the combinations of norfloxacin and dioxidine according to recipes 1 and 3.

The fractional inhibitory concentration (FIC) of norfloxacin in the combination of norfloxacin and dioxidine according to formulation 2 in the experiment with Salmonella cholerae suis was 0.450, dioxidine - 0.004.

The FIC index (the sum of the fractional inhibitory concentrations of the components) of the combination of norfloxacin and dioxidine in the preparation for the treatment of gastrointestinal diseases of young farm animals according to formulation 2 in the experiment with the Salmonella cholerae suis culture was 0.454.

Example 2. The study of the optimal ratio of norfloxacin and dioxidine in a preparation for the treatment of gastrointestinal diseases of young farm animals in in vivo experiments.

The optimal ratio of norfloxacin and dioxidine in a preparation for the treatment of gastrointestinal diseases of young farm animals was studied in experiments on the treatment of colibacteriosis in piglets.

The diagnosis of colibacteriosis was established on the basis of data from a clinical examination of animals, autopsy, laboratory tests taking into account the epizootic situation on the farm. In the experiment on pigs in the treatment of colibacteriosis, the higher effectiveness of the drug for the treatment of gastrointestinal diseases of young farm animals in the following ratio of components, wt.%:

Norfloxacin - 9.0;

Dioxidine - 1.0;

1.2 Propylene glycol - 19.8;

Polyvinylpyrrolidone low molecular weight - 0.2;

Distilled water - the rest.

The research results are presented in table 2.

table 2 The effectiveness of the drug for the treatment of gastrointestinal diseases of young farm animals with different ratios of norfloxacin and dioxidine in the treatment of colibacteriosis in piglets. Indicators Drug formulation one 2 3 Number of animals, goal. 44 45 46 Duration of treatment, days 3.8 ± 0.8 3.6 ± 0.4 3.9 ± 0.5 Recover, goal.
% 40
90.9 42
93.3 41
89.1 Remained sick, goal.
% 3
6.8 2
4.4 2
4.3 It’s a goal.
% one
2,3 one
2.2 3
6.5 Therapeutic efficacy,% 90.9 93.3 89.1 Designations in the table:
1 - Recipe 1 drug for the treatment of gastrointestinal diseases of young farm animals with a ratio of components: Norfloxacin - 9.0; Dioxidine - 0.5; 1.2 Propylene glycol - 19.8; Polyvinylpyrrolidone low molecular weight - 0.2; Distilled water - the rest.
2 - Formulation 2 of the drug for the treatment of gastrointestinal diseases of young farm animals with a ratio of components: Norfloxacin - 9.0; Dioxidine - 1; 1.2 Propylene glycol - 19.8; Polyvinylpyrrolidone low molecular weight - 0.2; Distilled water - the rest.
3 - Formulation 3 of the drug for the treatment of gastrointestinal diseases of young farm animals with a ratio of components: Norfloxacin - 8; Dioxidine - 1; 1.2 Propylene glycol - 19.8; Polyvinylpyrrolidone low molecular weight - 0.2; Distilled water - the rest.

Example 3. Antimicrobial activity of the drug for the treatment of gastrointestinal diseases of young farm animals.

The study of the antimicrobial activity of the drug for the treatment of gastrointestinal diseases of young farm animals was carried out by the method of serial dilutions. As test cultures, reference and field (pathogenic) strains of microorganisms - pathogens and gastrointestinal diseases of piglets and calves typified by morphological, tinctorial, cultural, biochemical and serological properties were used. As a liquid nutrient medium, meat-peptone broth was used.

The main solution of the drug for the treatment of gastrointestinal diseases of young farm animals is 1000 μg / ml. The drug was taken in the optimal ratio of components. A working solution was prepared from the main solution of the preparation. 4.5 ml of meat-peptone broth were poured into 16 sterile tubes. 4.5 ml of the preparation was added to 1 tube, mixed, after which 4.5 ml of the resulting solution was transferred to the next tube, thus continuing dilution to 15 tubes. As a control of culture growth, a 16 test tube containing no preparation was used.

Table 3 Antimicrobial activity of the drug for the treatment of gastrointestinal diseases of young farm animals (μg / ml) Microorganism culture MBSK MBCC E.coli K 88 (p) 0.06 0.12 E.coli 08 0,03 0.06 E.coli 0142 (p) 0.06 0.12 E.coli A 20 0,03 0.06 Salmonella cholerae suis 0.015 0.06 Salmonella cholerae suis (n) 0,03 0.06 S. Dublin 0,03 0.06 S.thyphimurium 0,03 0.06 Pseudomonas aeruginosae 0.06 0.12

The content of microbial cells in one ml of medium was 500 thousand.

Crops were incubated at 37 ^about C for 18-20 hours. After the incubation period, the result was taken into account.

The minimum bacteriostatic concentration (MBsK) of the drug for the treatment of gastrointestinal diseases of young farm animals was determined by the method of serial dilutions in meat and peptone broth (MPB); minimum bactericidal concentration - by seeding from tubes with a transparent medium on solid nutrient media (MPA) that do not contain the drug.

The results of the studies are presented in table 3.

From table 3 it is seen that the drug has a pronounced antimicrobial effect in relation to the tested cultures of microorganisms. For museum and field strains of Salmonella and Escherichia coli, the minimum inhibitory concentration was 0.015-0.06 μg / ml.

Example 4. The study of the effectiveness of the treatment of colibacillosis in calves.

The study of the effectiveness of the drug for the treatment of colibacteriosis in calves was carried out in two experiments on calves 1-10 days of age weighing 35-45 kg.

According to the principle of paired analogues, animals were divided into two groups.

The diagnosis of colibacteriosis was established comprehensively on the basis of data from a clinical examination of animals, laboratory tests, postmortem autopsy, taking into account the epizootic situation on the farm.

The animals were monitored daily for 10-15 days, taking into account the general condition, mortality, growth rate, and timing of recovery.

The first experiment to study the effectiveness of the drug for the treatment of colibacillosis of calves was carried out in the SCA them. Dokuchaev Talov district of the Voronezh region.

The calves of the control group (67 goals) were treated with the base drug Enroxil 5% [10], which was administered subcutaneously at a dose of 1 ml per 20 kg of body weight (2.5 mg of enrofloxacin per 1 kg of body weight) once a day for 3 days .

The animals of the experimental group (71 animals) were treated with the preparation for the treatment of gastrointestinal diseases of young farm animals subcutaneously at a dose of 0.5 ml / 10 kg of body weight 1 time per day for 3 days.

The results of the experiment to study the effectiveness of the treatment of colibacteriosis in calves are presented in table 4.

Table 4 The effectiveness of the treatment of colibacillosis in calves Indicators Group of animals Control Experienced Number of animals, goals
Recover, Goals
%
Fallen, goals
%
Duration of recovery, days
Therapeutic efficacy,% 67
58
86.6
9
11.9
4.2 ± 0.3
86.6 71
64
90.1
7
8.5
3.6 ± 0.4
90.1

The second experience in studying the effectiveness of the parenteral use of the drug for the treatment of colibacteriosis in calves was carried out in the Kolos agricultural school in the Talovsky district of the Voronezh region.

The calves of the control group (53 animals) were treated with 5% enroxil, which was administered subcutaneously at a dose of 1 ml per 20 kg of body weight (2.5 mg of enrofloxacin per 1 kg of body weight) once a day for 3 days.

The animals of the experimental group (54 animals) were used the drug for the treatment of gastrointestinal diseases of young farm animals subcutaneously at a dose of 0.5 ml / 10 kg of body weight 1 time per day for 3 days.

The results of the experience of studying the effectiveness for the treatment of colibacillosis in calves are presented in table 5.

Studies have shown that the drug for the treatment of gastrointestinal diseases of young farm animals has high therapeutic efficacy in the treatment of colibacillosis in calves. Compared with the control groups, the use of the drug reduced the recovery period and death of animals.

Table 5 The effectiveness of the treatment of colibacillosis in calves Indicators Group of animals Control Experienced Number of animals, goals
Recover, Goals
%
Fallen, goals
%
Duration of recovery, days
Therapeutic efficacy,% 53
48
90.6
5
9,4
4.1 ± 0.4
90.6 54
fifty
92.6
four
7.4
3.9 ± 0.7
92.6

Example 5. The effectiveness of the treatment of salmonellosis in calves.

The study of the effectiveness of treatment of salmonellosis in calves was carried out in two experiments on calves 30-40 days of age weighing 50-70 kg.

According to the principle of paired analogues, animals were divided into two groups.

The diagnosis of salmonellosis was established comprehensively on the basis of data from a clinical examination of animals, laboratory tests, postmortem autopsy, taking into account the epizootic situation on the farm.

The animals were monitored daily for 10-15 days, taking into account the general condition, mortality, growth rate, and timing of recovery.

The first experience in studying the effectiveness of treatment of calf salmonellosis was carried out in the agricultural school Zarya, Talovsky district, Voronezh region.

Animals of the control group (66 animals) were treated with 5% enroxil, which was administered subcutaneously at a dose of 1 ml per 20 kg of body weight (2.5 mg of enrofloxacin per 1 kg of body weight) once a day for 5 days.

The animals of the experimental group (60 animals) were used the drug for the treatment of gastrointestinal diseases of young farm animals subcutaneously at a dose of 0.5 ml / 10 kg of body weight 1 time per day for 5 days.

The results of the experiment to study the effectiveness of treatment of salmonellosis in calves are presented in table 6.

Table 6 The effectiveness of the treatment of salmonellosis in calves Indicators Group of animals Control Experienced Number of animals, goals
Recover, Goals
%
Fallen, goals
%
Stayed sick goals
%
Duration of recovery, days
Therapeutic efficacy,% 66
59
89.4
2
3.0
5
7.6
5.6 ± 0.7
89.4 60
54
90.0
2
3.3
four
6.7
5.5 ± 0.5
90.0

The second experience in studying the effectiveness of treatment of calf salmonellosis was carried out in OAO Slavs of the Talovsky District of the Voronezh Region.

Animals of the control group (32 animals) were treated with 5% enroxil, which was administered subcutaneously at a dose of 1 ml per 20 kg of body weight (2.5 mg of enrofloxacin per 1 kg of body weight) once a day for 5 days.

The animals of the experimental group (40 goals.) Used the drug for the treatment of gastrointestinal diseases of young farm animals subcutaneously at a dose of 0.5 ml / 10 kg of body weight 1 time per day for 5 days.

The results of an experiment to study the effectiveness for the treatment of salmonellosis in calves are presented in table 7.

Table 7 The effectiveness of the treatment of salmonellosis in calves Indicators Group of animals Control Experienced Number of animals, goals
Recover, Goals
%
Fallen, goals
%
Stayed sick goals
%
Duration of recovery, days
Therapeutic efficacy,% 32
29th
90.6
-
-
3
9,4
5.4 ± 0.4
90.6 40
37
92.5
-
-
3
7.5
3.9 ± 0.5
92.5

Studies have shown that the drug for the treatment of gastrointestinal diseases of young farm animals has high therapeutic efficacy in the treatment of salmonellosis in calves. In all experiments, when using the drug for the treatment of gastrointestinal diseases of young farm animals, the recovery and death rates of animals were lower compared with the control.

Example 6. The effectiveness of the treatment of colibacteriosis in piglets.

The study of the effectiveness of the treatment of colibacillosis in piglets was carried out in two experiments on pigs of 7-10 days of age weighing 2.5-3.5 kg.

According to the principle of paired analogues, animals were divided into two groups.

The diagnosis of colibacteriosis was established comprehensively on the basis of data from a clinical examination of animals, laboratory tests, postmortem autopsy, taking into account the epizootic situation on the farm.

The animals were monitored daily for 10-15 days, taking into account the general condition, mortality, growth rate, and timing of recovery.

The first experience in studying the effectiveness of the treatment of colibacillosis of piglets was carried out in the SCA "Beginning" Rossoshansky district of the Voronezh region.

Piglets of the control group (56 animals) were treated with 5% enroxil, which was administered intramuscularly at a dose of 1 ml per 20 kg of body weight (2.5 mg of enrofloxacin per 1 kg of body weight) once a day for 3 days.

The animals of the experimental group (67 goals) were used the drug for the treatment of gastrointestinal diseases of young farm animals intramuscularly at a dose of 0.5 ml / 10 kg of body weight 1 time per day for 3 days.

The results of an experiment to study the effectiveness for the treatment of colibacillosis in piglets are presented in table 8.

The second experiment was conducted in the agricultural association “Aleksandrovskoye” in the Rossoshansky district of the Voronezh region.

Piglets of the control group (83 animals) were treated with 5% enroxil, which was administered intramuscularly at a dose of 1 ml per 20 kg of body weight (2.5 mg of enrofloxacin per 1 kg of body weight) once a day for 3 days.

The animals of the experimental group (90 animals) were treated with the preparation for the treatment of gastrointestinal diseases of young farm animals intramuscularly at a dose of 0.5 ml / 10 body weight 1 time per day for 3 days.

Table 8 The effectiveness of the treatment of colibacillosis in piglets Indicators Group of animals Control Experienced Number of animals, goals
Recover, Goals
%
Fallen, goals
%
Stayed sick goals
%
Duration of recovery, days
Therapeutic efficacy,% 56
51
91.1
5
8.9
2
3.6
5.8 ± 0.4
91.1 67
63
94.0
four
6.0
2
3.0
4.9 ± 0.4
94.0

Table 9 The effectiveness of the treatment of colibacillosis in piglets Indicators Group of animals Control Experienced Number of animals, goals
Recover, Goals
%
Fallen, goals
%
Duration of recovery, days
Therapeutic efficacy,% 83
76
91.6
7
8.4
5.8 ± 0.3
90,4 90
86
95.6
four
4.4
4.7 ± 0.6
93.3

The results of the experiment to study the effectiveness of the treatment of colibacteriosis in piglets are presented in table 9.

As studies have shown, the drug for the treatment of gastrointestinal diseases of young farm animals has high therapeutic efficacy in the treatment of colibacillosis in piglets. In all experiments, when using the drug for the treatment of gastrointestinal diseases of young farm animals, the recovery time and mortality of animals decreased compared with the control groups.

Example 7. The effectiveness of the treatment of salmonellosis in piglets.

The study of the effectiveness of treatment of salmonellosis in piglets was carried out in two experiments on pigs 30-45 days of age weighing 8.5-11.0 kg.

According to the principle of paired analogues, animals were divided into two groups.

The diagnosis of salmonellosis was established comprehensively on the basis of data from a clinical examination of animals, laboratory tests, postmortem autopsy, taking into account the epizootic situation on the farm.

The animals were monitored daily for 10-15 days, taking into account the general condition, mortality, growth rate, and timing of recovery.

The first experience in studying the effectiveness of the treatment of salmonellosis in piglets was carried out in the State Unitary Enterprise “Znamya Oktyabrya”, Talovsky District, Voronezh Region.

Animals of the control group (76 animals) were treated with 5% enroxil, which was administered intramuscularly at a dose of 1 ml per 20 kg of body weight (2.5 mg of enrofloxacin per 1 kg of body weight) once a day for 5 days.

The animals of the experimental group (83 animals) were used the drug for the treatment of gastrointestinal diseases of young farm animals intramuscularly at a dose of 0.5 ml / 10 body weight 1 time per day for 5 days.

The results of an experiment to study the effectiveness for the treatment of salmonellosis in piglets are presented in table 10.

The second experience in studying the effectiveness of salmonellosis treatment was carried out in the Slavyanskoe Agricultural Association of the Talovsk District of the Voronezh Region.

Table 10 The effectiveness of the treatment of salmonellosis in piglets Indicators Group of animals Control Experienced Number of animals, goals 76 83 Recover, Goals 70 78 % 92.1 94.0 Fallen, goals 3 2 % 3.9 2,4 Stayed sick goals 3 3 % 3.9 3.6 Duration of recovery, days 5.7 ± 0.4 5.1 ± 0.6 Therapeutic efficacy,% 92.1 94.0

Animals of the control group (108 animals) were treated with 5% enroxil, which was administered intramuscularly at a dose of 1 ml per 20 kg of body weight (2.5 mg of enrofloxacin per 1 kg of body weight) once a day for 5 days.

The animals of the experimental group (129 animals) were treated with the preparation for the treatment of gastrointestinal diseases of young farm animals intramuscularly at a dose of 0.5 ml / 10 body weight 1 time per day for 5 days.

The results of the experiment to study the effectiveness of the treatment of salmonellosis in piglets are presented in table 11.

Table 11 The effectiveness of the treatment of salmonellosis in piglets Indicators Group of animals Control Experienced Number of animals, goals
Recover, Goals
%
Fallen, goals
%
Stayed sick goals
%
Duration of recovery, days
Therapeutic efficacy,% 108
99
91.7
one
0.9
8
7.4
5.3 ± 0.6
91.6 129
120
93.0
one
0.8
8
6.2
4.9 ± 0.3
93.0

Studies have shown that the drug for the treatment of gastrointestinal diseases of young farm animals has high therapeutic efficacy in the treatment of salmonellosis in piglets. Compared with the control groups, when using the drug for the treatment of gastrointestinal diseases of young farm animals, the recovery period and mortality of the animals decreased.

Tests of the drug for the treatment of gastrointestinal diseases of young farm animals have shown its high efficiency.

Based on the results of the studies, it was found that the drug for the treatment of gastrointestinal diseases of young farm animals has a pronounced antimicrobial and therapeutic effect in gastrointestinal diseases of bacterial etiology in piglets and calves.

The use of the drug is technologically and cost-effective.

Information sources

1. RU, 2105548 C1, 02.27.1998.

2. RU, 2075972 C1, 03/27/1997.

3. Mashkovsky M.D. Medicines In two parts. Part 2. - ed. reslave. and add. - M .: Medicine, 1993. - P.353.

4. British Pharmacopoiea, 2000, v. 1, p. 974.

5. Instructions for the use of dioxidine, USSR Ministry of Health. 12/23/1982

6. Mashkovsky M.D. Medicines In two parts. Part 2. - ed. reslave. and add. - M .: Medicine, 1993. - S.356-357.

7. Polydec-Fabini R., Beirich T. Organic analysis: Per. with him. - L .: Chemistry, 1981. - P.68-69.

8. British Pharmacopoiea, 2000, v. 1, p. 1155.

9. Polydec-Fabini R., Beirich T. Organic analysis: Per. with him. - L .: Chemistry, 1981. - S.528-529.

10. Veterinary drugs in Russia: a guide in 2 volumes. T. 1 / I.F.Klenova, K.L. Maltsev, N.A. Eremenko, A.I. Arkhipov. - Agricultural Publishing House, 2004.

Claims (1)

A drug for the treatment of gastrointestinal diseases of young farm animals, including dioxidin, characterized in that it additionally contains norfloxacin, 1,2-propylene glycol, low molecular weight polyvinylpyrrolidone and distilled water in the following ratio, wt.%: norfloxacin 9.0 dioxidine 1,0 1,2-propylene glycol 19.8 low molecular weight polyvinylpyrrolidone 0.2 distilled water rest