RU2633747C1 - Method of obtaining nanocapules of salvia hispanica in hellan gum - Google Patents

Abstract

FIELD: nanotechnology.

SUBSTANCE: salvia hispanica powder is slowly added to a suspension of gellan gum in hexane in the presence of 0.01 g E472c as a surfactant, then is stirred at 1000 rpm, after chloroform is added, after which the resulting suspension is filtered and dried at a room temperature, wherein the core:shell mass ratio is 1:1 or 1:3 or 3:1.

EFFECT: simplification and acceleration of the process of obtaining nanocapsules, reducing losses in the obtaining nanocapsules and the possibility of use in the food industry.

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Description

The invention relates to the field of nanotechnology and the food industry.

Previously known methods for producing microcapsules.

In US Pat. 2173140, IPC A61K 009/50, A61K 009/127, Russian Federation, published September 10, 2001. A method for producing silicon-organolipid microcapsules using a rotary-cavitation unit with high shear forces and powerful sonar acoustic and ultrasonic dispersion ranges is proposed.

The disadvantage of this method is the use of special equipment - a rotary cavitation unit, which has an ultrasonic effect, which affects the formation of microcapsules and can cause adverse reactions due to the fact that ultrasound destructively affects polymers of a protein nature, therefore, the proposed method is applicable when work with polymers of synthetic origin

In US Pat. 2359662, IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00, published June 27, 2009, Russian Federation, a method for producing sodium chloride microcapsules using spray cooling in a Niro spray cooling tower under the following conditions: temperature inlet air 10 ° C, outlet air temperature 28 ° C, spray drum rotation speed 10,000 rpm. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (air temperature at the inlet 10 ° C, air temperature at the outlet 28 ° C, rotation speed of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. 2134967, IPC A01N 53/00, A01N 25/28, published on 08.27.1999, Russian Federation (1999). A solution of a mixture of natural lipids and a pyrethroid insecticide in a weight ratio of 2-4: 1 in an organic solvent is dispersed in water, which simplifies the microencapsulation method.

The disadvantage of this method is dispersion in an aqueous medium, which makes the proposed method inapplicable for producing microcapsules of water-soluble preparations in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reduce losses in obtaining nanocapsules (increase in yield by mass).

The solution to the technical problem is achieved by the method of producing nanocapsules of chia seeds, characterized in that gellan gum is used as the shell of the nanocapsules, and chia seeds are used as the core when nanocapsules are prepared by non-solvent precipitation using chloroform as a precipitant.

A distinctive feature of the proposed method is the preparation of nanocapsules by non-solvent precipitation using chloroform as a precipitant, as well as the use of gellan gum as a shell of particles and chia seeds as a core.

EXAMPLE 1

Obtaining nanocapsules of chia seeds in the ratio of core: shell 1: 1

1 g of powdered chia seeds is slowly added to a suspension of 1 g of gellan gum in hexane in the presence of 0.01 g of the preparation E472c (glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid, with citric acid as a tribasic, can be esterified with other glycerides and as an acid with other fatty acids. Free acid groups can be neutralized with sodium) as a surfactant with stirring at 1000 rpm. Then pour 6 ml of chloroform. The resulting suspension is filtered and dried at room temperature.

Received 2 g of nanocapsule powder. The yield was 100%.

EXAMPLE 2

Obtaining nanocapsules of chia seeds in the ratio of core: shell 1: 3

1 g of powdered chia seeds is slowly added to a suspension of 3 g of gellan gum in hexane in the presence of 0.01 g of the preparation E472c as a surfactant with stirring at 1000 rpm. Then pour 8 ml of chloroform. The resulting suspension is filtered and dried at room temperature.

Received 4 g of nanocapsule powder. The yield was 100%.

EXAMPLE 3

Obtaining nanocapsules of chia seeds in the ratio of core: shell 3: 1

3 g of powdered chia seeds are slowly added to a suspension of 1 g of gellan gum in hexane in the presence of 0.01 g of the preparation E472c as a surfactant with stirring at 1000 rpm. Then pour 8 ml of chloroform. The resulting suspension is filtered and dried at room temperature.

Received 4 g of nanocapsule powder. The yield was 100%.

EXAMPLE 4

Sizing nanocapsules by NTA.

The measurements were carried out on a Nanosight LM0 multiparameter nanoparticle analyzer manufactured by Nanosight Ltd (Great Britain) in the HS-BF configuration (Andor Luca high-sensitivity video camera, 405 nm semiconductor laser with a power of 45 mW). The device is based on the Nanoparticle Tracking Analysis (NTA) method described in ASTM E2834.

The optimal dilution for dilution was 1: 100. For the measurement, the device parameters were selected: Camera Level = 16, Detection Threshold = 10 (multi), Min Track Length: Auto, Min Expected Size: Auto. duration of a single measurement of 215s, the use of a syringe pump.

Claims (1)

A method of producing nanocapsules of chia seeds in gellan gum, characterized in that gellan gum is used as a shell of nanocapsules, while chia seed powder is slowly added to a suspension of gellan gum in hexane in the presence of 0.01 g of E472c as a surfactant, then mixed at 1000 rpm, then chloroform is poured, after which the resulting suspension is filtered off and dried at room temperature, while the mass ratio of the core: shell is 1: 1, or 1: 3, or 3: 1.

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