RU2626828C1 - Method of producing nanocapsules of reservoir in kappa-carrageenan - Google Patents

Abstract

FIELD: nanotechnology.

SUBSTANCE: kappa-carrageenan is used as the nanocapsule shell, and resveratrol is used as the core for the mass ratio of the shell: the core is 3:1 and 1:5. Resveratrol is slowly added to the kappa-carrageenan slurry in butanol in the presence of 0.01 g E472 c as a surfactant with stirring at 1000 rpm, then butyl chloride is added. The resulting suspension is filtered and dried at the room temperature.

EFFECT: nanocapsules production process simplification and acceleration, reduction of losses during the nanocapsules production.

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Description

The invention relates to the field of nanotechnology and the food industry.

Previously known methods for producing microcapsules.

In US Pat. 2173140 IPC A61K 009/50, A61K 009/127, Russian Federation, published September 10, 2001. A method for producing silicon organolipid microcapsules using a rotary-cavitation unit with high shear forces and powerful sonar acoustic and ultrasonic dispersion ranges is proposed.

The disadvantage of this method is the use of special equipment - a rotary cavitation unit, which has an ultrasonic effect, which affects the formation of microcapsules and can cause adverse reactions due to the fact that ultrasound destructively affects polymers of a protein nature, therefore, the proposed method is applicable when work with polymers of synthetic origin.

In US Pat. 2359662 IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00, published June 27, 2009, Russian Federation, a method for producing sodium chloride microcapsules using spray cooling in a Niro spray cooling tower under the following conditions: air temperature at the inlet 10 ° C, outlet air temperature 28 ° C, the rotation speed of the spray drum 10,000 rpm. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (air temperature at the inlet 10 ° C, air temperature at the outlet 28 ° C, rotation speed of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. 2134967 IPC A01N 53/00, A01N 25/28, published 08/27/1999, Russian Federation (1999). A solution of a mixture of natural lipids and a pyrethroid insecticide in a weight ratio of 2-4: 1 in an organic solvent is dispersed in water, which simplifies the microencapsulation method.

The disadvantage of this method is dispersion in an aqueous medium, which makes the proposed method inapplicable for producing microcapsules of water-soluble preparations in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reduce losses in obtaining nanocapsules (increase in yield by mass).

The solution to the technical problem is achieved by the method of producing resveratrol nanocapsules, characterized in that kappa-carrageenan is used as the nanocapsule shell, and resveratrol is used as the core when nanocapsules are prepared by the non-solvent precipitation method using butyl chloride as a precipitant.

A distinctive feature of the proposed method is the preparation of nanocapsules by non-solvent precipitation using butyl chloride as a precipitant, as well as the use of kappa-carrageenan as a particle shell and resveratrol as a core.

The result of the proposed method are the preparation of resveratrol nanocapsules.

EXAMPLE 1 Obtaining nanocapsules of resveratrol, the ratio of the shell: core 3: 1

1 g of resveratrol is slowly added to a suspension of 3 g of kappa-carrageenan in butanol in the presence of 0.01 g of the preparation E472 s (glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid, with citric acid as a tribasic, can be esterified with other glycerides and as an acid with other fatty acids. Free acid groups can be neutralized with sodium) as a surfactant with stirring at 1000 rpm. Next, 5 ml of butyl chloride are added. The resulting suspension is filtered and dried at room temperature.

Received 4 g of nanocapsule powder. The yield was 100%.

EXAMPLE 2 Obtaining nanocapsules of resveratrol, the ratio of the shell: core 1: 5

5 g of resveratrol is slowly added to a suspension of 1 g of kappa-carrageenan in butanol in the presence of 0.01 g of the preparation E472 c as a surfactant with stirring at 1000 rpm. Then 3 ml of butyl chloride are added. The resulting suspension is filtered and dried at room temperature.

Received 6 g of nanocapsule powder. The yield was 100%.

EXAMPLE 3 Determination of the size of nanocapsules by NTA.

The measurements were carried out on a Nanosight LM0 multiparameter nanoparticle analyzer manufactured by Nanosight Ltd (Great Britain) in the HS-BF configuration (Andor Luca high-sensitivity video camera, 405 nm semiconductor laser with a power of 45 mW). The device is based on the method of analysis of trajectories of nanoparticles (Nanoparticle Tracking Analysis, NTA) described by bASTM E2834.

The optimal dilution for dilution was 1: 100. For measurement, the device parameters were selected: Camera Level = 16, Detection Threshold = 10 (multi), Min Track Length: Auto, Min Expected Size: Auto, duration of a single measurement 215s, use of a syringe pump.

Claims (1)

A method of producing resveratrol nanocapsules in kappa-carrageenan, characterized in that kappa-carrageenan is used as the shell of the nanocapsules, and resveratrol is used as the core in the shell: core mass ratio of 3: 1 and 1: 5, while resveratrol is slowly added to the kappa suspension β-carrageenan in butanol in the presence of 0.01 g of E472 s as a surfactant with stirring at 1000 rpm, then butyl chloride is added, after which the resulting suspension is filtered off and dried at room temperature.

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