RU2613883C1 - Process of getting rosemary nanocapsules in sodium alginate - Google Patents

Abstract

FIELD: nanotechnology.

SUBSTANCE: for shells of nanocapsules sodium alginate is used, and the rosemary powder is slowly added to a suspension of sodium alginate in petroleum ether in the presence of 0.01 g E472c as a surfactant, and then stirred at 1000 r/min and the ethyl acetate is poured, the resulting suspension is filtered and dried at room temperature, wherein the weight ratio of core: shell in nanocapsules is 1:1 or 1:3.

EFFECT: simplification and acceleration of the process of producing the nanocapsules of rosemary, as well as increase their yield by weight.

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Description

The invention relates to the field of nanotechnology and the food industry.

Previously known methods for producing microcapsules.

In US Pat. 2173140, IPC A61K 009/50, A61K 009/127, Russian Federation, published September 10, 2001. A method for producing silicon organolipid microcapsules using a rotary-cavitation unit with high shear forces and powerful sonar acoustic and ultrasonic dispersion ranges is proposed.

The disadvantage of this method is the use of special equipment - a rotary cavitation unit, which has an ultrasonic effect, which affects the formation of microcapsules and can cause adverse reactions due to the fact that ultrasound destructively affects polymers of a protein nature, therefore, the proposed method is applicable when work with polymers of synthetic origin.

In US Pat. 2359662, IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00, published June 27, 2009. The Russian Federation proposes a method for producing microcapsules of sodium chloride using spray cooling in a Niro spray cooling tower under the following conditions: air temperature at the inlet 10 ° С, air temperature at the outlet 28 ° С, rotation speed of the spraying drum 10,000 rpm. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (air temperature at the inlet 10 ° C, air temperature at the outlet 28 ° C, rotation speed of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. 2134967, IPC A01N 53/00, A01N 25/28, published on 08.27.1999, Russian Federation (1999). A solution of a mixture of natural lipids and a pyrethroid insecticide in a weight ratio of 2-4: 1 in an organic solvent is dispersed in water, which simplifies the microencapsulation method.

The disadvantage of this method is dispersion in an aqueous medium, which makes the proposed method inapplicable for producing microcapsules of water-soluble preparations in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reduce losses in obtaining nanocapsules (increase in yield by mass).

The solution to the technical problem is achieved by the method of producing rosemary nanocapsules, characterized in that sodium alginate is used as the nanocapsule shell, and rosemary is used as the core when nanocapsules are prepared by the non-solvent precipitation method using ethyl acetate as a precipitant.

A distinctive feature of the proposed method is the preparation of nanocapsules by non-solvent precipitation using ethyl acetate as a precipitant, as well as the use of sodium alginate as a particle shell and rosemary as a core.

EXAMPLE 1. Obtaining nanocapsules of rosemary in the ratio of the core: bog 1: 1

100 mg of rosemary is slowly added to a suspension of 100 mg of sodium alginate in petroleum ether in the presence of 0.01 g of the preparation E472 s (glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid, and citric acid as a tribasic can be etherified with other glycerides and as an acid with other fatty acids. Free acid groups can be neutralized with sodium as a surfactant with stirring at 1000 rpm. Next, pour 2 ml of ethyl acetate. the irradiated suspension is filtered off and dried at room temperature.

Obtained 0.2 g of nanocapsule powder. The yield was 100%.

EXAMPLE 2. Obtaining nanocapsules of rosemary in the ratio of core: shell 1: 3

500 mg of rosemary is slowly added to a suspension of 1.5 g of sodium alginate in petroleum ether in the presence of 0.01 g of the preparation E472c as a surfactant with stirring at 1000 rpm. Next, 2 ml of ethyl acetate are added. The resulting suspension is filtered and dried at room temperature.

Received 2 g of nanocapsule powder. The yield was 100%.

EXAMPLE 3. Determination of the size of nanocapsules by NTA

The measurements were carried out on a Nanosight LM0 multiparameter nanoparticle analyzer manufactured by Nanosight Ltd (Great Britain) in the HS-BF configuration (Andor Luca high-sensitivity video camera, 405 nm semiconductor laser with a power of 45 mW). The device is based on the Nanoparticle Tracking Analysis (NTA) method described in ASTM E2834.

The optimal ratio for dilution was chosen 1: 100. For measurement, the device parameters were selected: Camera Level = 16, Detection Threshold = 10 (multi), Min Track Length: Auto, Min Expected Size: Auto, duration of a single measurement 215s, use of a syringe pump.

Claims (1)

A method of producing rosemary nanocapsules in sodium alginate, characterized in that sodium alginate is used as the nanocapsule shell, while rosemary powder is slowly added to a suspension of sodium alginate in petroleum ether in the presence of 0.01 g of E472c as a surfactant, then mixed with 1000 rpm, then ethyl acetate is poured, after which the resulting suspension is filtered off and dried at room temperature, while the mass ratio of the core: shell in nanocapsules is 1: 1 or 1: 3.

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