RU2591802C1 - Method of producing nanocapsules of green tea extract - Google Patents

Abstract

FIELD: nanotechnology.

SUBSTANCE: invention relates to a method of producing nanocapsules and describes a method of producing nanocapsules of green tea extract. Method is characterised by using sodium carboxymethylcellulose as a shell and green tea extract - as a core; during the method implementation green tea extract is added to the suspension of sodium carboxymethylcellulose in butanol in presence of E472c surfactant, herewith the ratio of core:shell in terms of recalculation to dry substance is 1:1 or 1:3, or 1:5, then while stirring methylene chloride is added and the obtained suspension is filtered and dried at room temperature.

EFFECT: method provides simplifying and accelerating the process of nanoencapsulation, while reducing accompanying loss.

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Description

The invention relates to the field of nanotechnology, medicine and the food industry.

Previously known methods for producing microcapsules.

In US Pat. 2173140, IPC A61K 009/50, A61K 009/127, Russian Federation, published September 10, 2001. A method for producing silicon organolipid microcapsules using a rotary-cavitation unit with high shear forces and powerful sonar acoustic and ultrasonic dispersion ranges is proposed.

The disadvantage of this method is the use of special equipment - a rotary cavitation unit, which has an ultrasonic effect, which affects the formation of microcapsules and can cause adverse reactions due to the fact that ultrasound destructively affects polymers of a protein nature, therefore, the proposed method is applicable when work with polymers of synthetic origin

In US Pat. 2359662, IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00, published 06/27/2009, Russian Federation, a method for producing sodium chloride microcapsules using spray cooling in a Niro spray cooling tower under the following conditions: air temperature at the inlet 10 ° C, outlet air temperature 28 ° C, the rotation speed of the spray drum 10,000 rpm. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (air temperature at the inlet 10 ° C, air temperature at the outlet 28 ° C, rotation speed of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. 2134967, IPC A01N 53/00, A01N 25/28, published on 08.27.1999, Russian Federation (1999). A solution of a mixture of natural lipids and a pyrethroid insecticide in a weight ratio of 2-4: 1 in an organic solvent is dispersed in water, which simplifies the microencapsulation method. The disadvantage of this method is dispersion in an aqueous medium, which makes the proposed method inapplicable for producing microcapsules of water-soluble preparations in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reduce losses in obtaining nanocapsules (increase in yield by mass).

The solution to the technical problem is achieved by the method of producing nanocapsules of green tea extract, characterized in that sodium carboxymethyl cellulose is used as the shell of the nanocapsules, and green tea extract is used as the core when nanocapsules are prepared by the non-solvent precipitation method using methylene chloride as a precipitant.

A distinctive feature of the proposed method is the preparation of nanocapsules by non-solvent precipitation using methylene chloride as a precipitant, as well as the use of sodium carboxymethyl cellulose as a particle shell and green tea extract as a core.

The result of the proposed method are obtaining nanocapsules of green tea extract.

The method is illustrated in Fig. one.

EXAMPLE 1. Obtaining nanocapsules of green tea extract, the ratio of the core: shell 1: 3

100 mg of green tea extract is added to a suspension of sodium carboxymethyl cellulose in benzene containing 300 mg of the polymer in the presence of 0.01 g of the preparation E472c (glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid, with citric acid, as tribasic, it can be esterified with other glycerides and as an acid with other fatty acids. Free acid groups can be neutralized with sodium) as a surfactant with stirring 1300 about bpm Next, 5 ml of methylene chloride are poured. The resulting suspension is filtered and dried at room temperature.

Obtained 0.4 g of nanocapsule powder. The yield was 100%.

EXAMPLE 2. Obtaining nanocapsules of green tea extract, the ratio of core: shell 1: 1

100 mg of green tea extract is added to a suspension of sodium carboxymethyl cellulose in benzene containing 100 mg of the indicated polymer in the presence of 0.01 g of the preparation E472c as a surfactant with stirring at 1300 rpm. Next, 5 ml of methylene chloride are added. The resulting suspension is filtered and dried at room temperature.

Obtained 0.2 g of nanocapsule powder. The yield was 100%.

EXAMPLE 3. Obtaining nanocapsules of green tea extract, the ratio of the core: shell 1: 5

100 mg of green tea extract is added to a suspension of sodium carboxymethyl cellulose in benzene containing 500 mg of the indicated polymer in the presence of 0.01 g of the preparation E472c as a surfactant with stirring at 1300 rpm. Then poured 7 ml of methylene chloride. The resulting suspension is filtered and dried at room temperature.

Received 0.6 g of nanocapsule powder. The yield was 100%.

EXAMPLE 4. Determination of the size of nanocapsules by the NTA method.

The measurements were carried out on a Nanosight LM0 multiparameter nanoparticle analyzer manufactured by Nanosight Ltd (Great Britain) in the HS-BF configuration (Andor Luca high-sensitivity video camera, 405 nm semiconductor laser with a power of 45 mW). The device is based on the method of analysis of trajectories of nanoparticles (Nanoparticle Tracking Analysis, NTA), described in ASTM E2834.

The optimal dilution for dilution was 1: 100. For the measurement, the device parameters were selected: Camera Level = 16, Detection Threshold = 10 (multi), Min Track Length: Auto, Min Expected Size: Auto. duration of a single measurement of 215s, the use of a syringe pump.

Claims (1)

A method of producing nanocapsules of green tea extract, characterized in that sodium carboxymethyl cellulose is used as a shell, and green tea extract is used as a core; in the process, green tea extract is added to a suspension of sodium carboxymethyl cellulose in butanol in the presence of surfactant E472c, while the ratio of core: shell when converted to dry matter is 1: 1, or 1: 3, or 1: 5, then methylene chloride is poured with stirring, the resulting suspension is filtered ovyvayut and dried at room temperature.