RU2690661C1 - Method of producing rhaponticum dry extract nanocapsules - Google Patents

Abstract

FIELD: nanotechnology.

SUBSTANCE: invention relates to nanotechnology, medicine and food industry. Method of producing nanocapsules of dry extract Rhaponticum is characterized by that dry extract Rhaponticum is added to a suspension of kappa-carrageenan in methanol in presence of 0.01 g of glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid as a surfactant while stirring at 800 rpm, then adding methylene chloride, obtained suspension of nanocapsules is filtered and dried at room temperature, wherein weight ratio of core:shell is 1:1, 1:2 or 1:3.

EFFECT: disclosed is a method of producing nanocapsules of a dry extract Rhaponticum.

1 cl, 3 ex

Description

The invention relates to the field of nanotechnology, medicine, pharmacology, veterinary medicine and the food industry.

Previously known methods for producing microcapsules.

In pat. 2173140 IPC А61K 009/50, А61K 009/127 Russian Federation published on 10.09.2001 a method for the production of silicon organoanalipid microcapsules using a rotor-cavitation unit with high shear forces and powerful sonar phenomena of the sonic and ultrasonic range for dispersion was proposed.

The disadvantage of this method is the use of special equipment - rotary-cavitation installation, which has an ultrasonic effect, which affects the formation of microcapsules and can cause side reactions due to the fact that ultrasound has a destructive effect on protein-type polymers, therefore, the proposed method is applicable to work with polymers of synthetic origin.

In pat. 2359662 IPC А61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00 published on 06/27/2009 The Russian Federation proposed a method of producing sodium chloride microcapsules using spray cooling in a Niro spray cooling tower under the following conditions: inlet air temperature 10 ° C, the air temperature at the outlet 28 ° C, the speed of rotation of the spray drum 10,000 revolutions / min. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (inlet air temperature 10 ° C, air outlet temperature 28 ° C, the speed of rotation of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. 2134967 IPC A01N 53/00, A01N 25/28 published 08/27/1999 Russian Federation (1999). A solution of a mixture of natural lipids and a pyrethroid insecticide is dispersed in water in a 2-4: 1 weight ratio in an organic solvent, which leads to a simplification of the method of microencapsulation.

The disadvantage of the method is dispersion in an aqueous medium, which makes the proposed method inapplicable for the production of microcapsules of water-soluble drugs in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reducing losses when obtaining nanocapsules (increase in mass yield).

The solution of the technical problem is achieved by the method of obtaining nanocapsules, characterized in that kappa-carrageenan is used as a shell of nanocapsules, and dry extract of leuzei is used as a core, upon receipt of nanocapsules by a non-solvent deposition method using methylene chloride as a precipitant.

A distinctive feature of the proposed method is the preparation of nanocapsules by the method of deposition by a non-solvent using methylene chloride as a precipitant, and also using kappa-carrageenan as a shell of particles and dry extract of levzey as a core.

The result of the proposed method is to obtain nanocapsules of dry extract of Leuzea.

EXAMPLE 1 Obtaining nanocapsules of dry leuzea extract, core: shell ratio 1: 3

1 g of a dry extract of a devsey is added to a suspension of 3 g of kappa-carrageenan in methanol in the presence of 0.01 g of E472c (an ester of glycerol with one or two edible fatty acid molecules and one or two citric acid molecules, and citric acid, as a tribasic, it can be esterified with other glycerides and as oxoacid with other fatty acids. Free acid groups can be neutralized by sodium) as a surfactant with stirring at 800 rpm. Next, pour 6 ml of methylene chloride. The resulting suspension is filtered and dried at room temperature.

Received 4 g of powder nanocapsules. The yield was 100%.

EXAMPLE 2 Obtaining nanocapsules of dry leuzea extract, core: shell ratio 1: 1

1 g of dry levzey extract is added to a suspension of 1 g of kappa-carrageenan in methanol in the presence of 0.01 g of E472c as a surfactant with stirring at 800 rpm. Next, pour 6 ml of methylene chloride. The resulting suspension is filtered and dried at room temperature.

Received 2 g of powder nanocapsules. The yield was 100%.

Example 3 Obtaining nanocapsules of dry extract of Leuzea, core: shell ratio 1: 2

1 g of dry levzei extract is added to a suspension of 2 g of kappa-carrageenan in methanol in the presence of 0.01 g of E472c as a surfactant with stirring at 800 rpm. Next, pour 6 ml of methylene chloride. The resulting suspension is filtered and dried at room temperature.

Received 3 g of powder nanocapsules. The yield was 100%.

Claims (1)

A method for preparing dry extracts of Leuzea nanocapsules, characterized in that dry extract of Leuzea is added to a suspension of kappa-carrageenan in methanol in the presence of 0.01 g of glycerol ester with one or two edible fatty acid molecules and one or two citric acid molecules as surface the active substance with stirring at 800 rpm, then methylene chloride is added, the resulting suspension of nanocapsules is filtered and dried at room temperature, while the mass ratio core: shell is 1: 1, 1: 2 or 1: 3