RU2724580C1 - Method for production of dry juniper extract nanocapsules - Google Patents

Abstract

FIELD: medicine; pharmaceuticals; food industry.

SUBSTANCE: invention refers to medicine, pharmaceutics and food industry and can be used for producing nanocapsules of juniper extract. Method of producing nanocapsules of dry juniper extract comprises adding dry juniper extract to a suspension of kappa-carrageenan in methanol in presence of 0.01 g of glycerol ester with one or two molecules of edible fatty acids and one or two molecules of citric acid as a surfactant while stirring at 600 rpm, then adding tetrachloromethane, obtained suspension of nanocapsules is filtered and dried at room temperature. Weight ratio of core : shell in produced nanocapsules makes 1:1, 1:2 or 1:3.

EFFECT: invention allows to simplify and accelerate the process of producing nanocapsules and increase the output by mass.

1 cl, 3 ex

Description

The invention relates to the field of nanotechnology, medicine, pharmacology and the food industry.

Previously known methods for producing microcapsules.

In US Pat. 2173140 IPC A61K 009/50, A61K 009/127 Russian Federation published September 10, 2001. A method for producing silicon organolipid microcapsules using a rotary-cavitation unit with high shear forces and powerful sonar and ultrasonic sonar acoustic dispersion ranges is proposed.

The disadvantage of this method is the use of special equipment - rotary cavitation unit, which has an ultrasonic effect, which affects the formation of microcapsules and can cause adverse reactions due to the fact that ultrasound destructively affects polymers of a protein nature, therefore, the proposed method is applicable when work with polymers of synthetic origin

In US Pat. 2359662 IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00 published on 06/27/2009 The Russian Federation proposed a method for producing sodium chloride microcapsules using spray cooling in a Niro spray cooling tower under the following conditions: inlet air temperature 10 ° C, outlet air temperature 28 ° C, the rotation speed of the spray drum 10,000 rpm. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (air temperature at the inlet 10 ° C, air temperature at the outlet 28 ° C, rotation speed of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. 2134967 IPC A01N 53/00, A01N 25/28 published on 08.27.1999 Russian Federation (1999). A solution of a mixture of natural lipids and a pyrethroid insecticide in a weight ratio of 2-4: 1 in an organic solvent is dispersed in water, which simplifies the microencapsulation method.

The disadvantage of this method is dispersion in an aqueous medium, which makes the proposed method inapplicable for producing microcapsules of water-soluble preparations in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reducing losses in obtaining nanocapsules (increase in yield by mass).

The solution to the technical problem is achieved by the method of producing nanocapsules, characterized in that kappa-carrageenan is used as the shell of the nanocapsules, and juniper dry extract is used as the core when nanocapsules are prepared by the non-solvent precipitation method using carbon tetrachloride as a precipitant.

A distinctive feature of the proposed method is the preparation of nanocapsules by non-solvent precipitation using carbon tetrachloride as a precipitant, as well as the use of kappa-carrageenan as a shell of particles and dry juniper extract as a core.

The result of the proposed method is the production of nanocapsules of dry juniper extract.

EXAMPLE 1 Obtaining nanocapsules of dry juniper extract, the ratio of core: shell 1: 3

1 g of dry extract of juniper is added to a suspension of 3 g of kappa-carrageenan in methanol in the presence of 0.01 g of the preparation E472c (glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid, with citric acid as a tribasic, can be esterified with other glycerides and as an acid with other fatty acids. Free acid groups can be neutralized with sodium) as a surfactant with stirring at 600 rpm. Next, 5 ml of carbon tetrachloride are added. The resulting suspension was filtered and dried at room temperature.

Received 4 g of nanocapsule powder. The yield was 100%.

EXAMPLE 2 Obtaining nanocapsules of dry juniper extract, the ratio of core: shell 1: 1

1 g of juniper dry extract is added to a suspension of 1 g of kappa-carrageenan in methanol in the presence of 0.01 g of the preparation E472c as a surfactant with stirring at 600 rpm. Next, 5 ml of carbon tetrachloride are added. The resulting suspension was filtered and dried at room temperature.

Received 2 g of nanocapsule powder. The yield was 100%.

Example 3 Preparation of Nanocapsules of Juniper Dry Extract, Core: Shell Ratio 1: 2

1 g of juniper dry extract is added to a suspension of 2 g of kappa-carrageenan in methanol in the presence of 0.01 g of the preparation E472c as a surfactant with stirring at 600 rpm. Next, 5 ml of carbon tetrachloride are added. The resulting suspension was filtered and dried at room temperature.

Received 3 g of nanocapsule powder. The yield was 100%.

Claims (1)

A method for producing nanocapsules of juniper dry extract, characterized in that the juniper dry extract is added to a suspension of kappa-carrageenan in methanol in the presence of 0.01 g of glycerol ester with one or two molecules of edible fatty acids and one or two molecules of citric acid as a surface the active substance with stirring at 600 rpm, carbon tetrachloride is poured, the resulting suspension of nanocapsules is filtered off and dried at room temperature, while the mass ratio of the core: shell is 1: 1, 1: 2 or 1: 3.