RU2694823C1 - Method for producing nanocapsules of dry extract of rhodiola quadrifida - Google Patents

Abstract

FIELD: nanotechnologies; medicine; food industry.

SUBSTANCE: invention relates to nanotechnology, medicine and food industry. Method for producing nanocapsules of dry extract of Rhodiola quadrifida is characterized by that nano capsules are represented by kappa-carrageenan, and as core – dry extract of Rhodiola quadrifida, wherein the dry extract of Rhodiola quadrifida is added to the kappa-carrageenan suspension in isopropanol in presence of 0.01 g of E472c as a surfactant while stirring at 700 rpm, acetonitrile is then poured, the resulting nanocapsule suspension is filtered and dried at room temperature, with the core:shell mass ratio being 1:1, 1:2 or 1:3.

EFFECT: disclosed is a method of producing nanocapsules of dry extract of Rhodiola quadrifida.

1 cl, 3 ex

Description

The invention relates to the field of nanotechnology, medicine, pharmacology, veterinary medicine, cosmetics and food industry.

Previously known methods for producing microcapsules.

In pat. 2173140 IPC A61K 009/50, A61K 009/127 Russian Federation published on 10.09.2001 a method for the preparation of silicon organoanalipid microcapsules was proposed using a rotary cavitation unit with high shear forces and powerful sonar phenomena of the sonic and ultrasonic range for dispersion.

The disadvantage of this method is the use of special equipment - rotary-cavitation installation, which has an ultrasonic effect, which affects the formation of microcapsules and can cause side reactions due to the fact that ultrasound has a destructive effect on protein-type polymers, therefore, the proposed method is applicable to work with polymers of synthetic origin,

In Pat.2359662 IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00 published on 06/27/2009 The Russian Federation proposed a method of producing sodium chloride microcapsules using spray cooling in a Niro spray cooling tower under the following conditions: air temperature at the inlet 10 ° С, the air temperature at the exit 28 ° С, the speed of rotation of the spraying drum 10,000 revolutions / min. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (inlet air temperature 10 ° С, outlet air temperature 28 ° С, rotation speed of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. 2134967 IPC A01N53 / 00, A01N25 / 28 published 08.27.1999 Russian Federation (1999). A solution of a mixture of natural lipids and a pyrethroid insecticide is dispersed in water in a 2-4: 1 weight ratio in an organic solvent, which leads to a simplification of the method of microencapsulation.

The disadvantage of the method is dispersion in an aqueous medium, which makes the proposed method inapplicable for the production of microcapsules of water-soluble drugs in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reducing losses when obtaining nanocapsules (increase in mass yield).

The solution of the technical problem is achieved by the method of obtaining nanocapsules, characterized in that kappa-carrageenan is used as a shell of nanocapsules, and a dry extract of a red brush is used as a core, upon receipt of nanocapsules by a non-solvent deposition method using acetonitrile as a precipitant.

A distinctive feature of the proposed method is the production of nanocapsules using a non-solvent deposition method using acetonitrile as a precipitant, as well as using kappa-carrageenan as a shell of particles and a dry extract of a red brush as a core.

The result of the proposed method is to obtain nanocapsules of dry extract of the red brush.

EXAMPLE 1 Preparation of nano-capsules of dry extract of red brush, core: shell ratio 1: 3.

1 g of a red brush dry extract is added to a suspension of 3 g of kappa-carrageenan in isopropanol in the presence of 0.01 g of E472c (an ester of glycerol with one or two edible fatty acid molecules and one or two citric acid molecules, and citric acid as the triazone , can be esterified with other glycerides and as oxoacid with other fatty acids. Free acid groups can be neutralized by sodium) as a surfactant with stirring at 700 rpm. Next, pour 7 ml of acetonitrile. The resulting suspension is filtered and dried at room temperature.

Received 4 g of powder nanocapsules. The yield was 100%.

EXAMPLE 2 Preparation of dry brush red extract nanocapsules, core: shell ratio 1: 1.

1 g of a dry red brush extract is added to a suspension of 1 g of kappa-carrageenan in isopropanol in the presence of 0.01 g of E472c as a surfactant with stirring at 700 rpm. Next, pour 7 ml of acetonitrile. The resulting suspension is filtered and dried at room temperature.

Received 2 g of powder nanocapsules. The yield was 100%.

Example 3 Preparation of dry brush red extract nanocapsules, core: shell ratio 1: 2.

1 g of dry extract of a red brush is added to a suspension of 2 g of kappa-carrageenan in isopropanol in the presence of 0.01 g of E472c as a surfactant with stirring at 700 rpm. Next, pour 7 ml of acetonitrile. The resulting suspension is filtered and dried at room temperature.

Received 3 g of powder nanocapsules. The yield was 100%.

Claims (1)

The method of obtaining dry extract red nanocapsules of the red brush, characterized by the fact that kappa-carrageenan is used as the shell of nanocapsules, and the dry extract of the red brush is used as the core, while the dry extract of the red brush is added to the suspension of kappa-carrageenan in isopropanol in the presence of 0.01 g E472s as a surfactant with stirring at 700 rpm, then acetonitrile is poured, the resulting nanocapsule suspension is filtered and dried at room temperature, while the mass ratio core: ca. ka is 1: 1, 1: 2 or 1: 3.