RU2622750C1 - Method for producing nanocapules of betulin in gellan gum - Google Patents

Abstract

FIELD: nanotechnology.

SUBSTANCE: method is characterized in that 100 mg of betulin powder is slowly added to a suspension of 100 mg or 300 mg of gellan gum in butanol in the presence of 0.01 g of E472c with stirring at 1300 rpm, then 3 ml of hexane are added, the resulting suspension is filtered and dried at room temperature.

EFFECT: simplification and acceleration of betulin nanocapsule production, weight yield increase.

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Description

The invention relates to the field of nanotechnology, agriculture and the food industry.

Previously known methods for producing microcapsules.

In US Pat. 2173140, IPC A61K 009/50, A61K 009/127, Russian Federation, publ. 09/10/2001 a method for producing silicon organolipid microcapsules using a rotary-cavitation unit with high shear forces and powerful sonar effects of sound and ultrasonic range for dispersion is proposed.

The disadvantage of this method is the use of special equipment - a rotary cavitation unit, which has an ultrasonic effect, which affects the formation of microcapsules and can cause adverse reactions due to the fact that ultrasound destructively affects polymers of a protein nature, therefore, the proposed method is applicable when work with polymers of synthetic origin.

In US Pat. 2359662, IPC A61K 009/56, A61J 003/07, B01J 013/02, A23L 001/00, publ. 06/27/2009, Russian Federation, a method for producing sodium chloride microcapsules using spray cooling in a Niro spray cooling tower is proposed under the following conditions: inlet air temperature 10 ° C, outlet air temperature 28 ° C, spray drum rotation speed of 10,000 rpm. The microcapsules of the invention have improved stability and provide controlled and / or prolonged release of the active ingredient.

The disadvantages of the proposed method are the duration of the process and the use of special equipment, a set of certain conditions (air temperature at the inlet 10 ° C, air temperature at the outlet 28 ° C, rotation speed of the spray drum 10,000 rpm).

The closest method is the method proposed in US Pat. 2134967, IPC A01N 53/00, A01N 25/28, publ. 08.27.1999, Russian Federation (1999). A solution of a mixture of natural lipids and a pyrethroid insecticide in a weight ratio of 2-4: 1 in an organic solvent is dispersed in water, which simplifies the microencapsulation method.

The disadvantage of this method is dispersion in an aqueous medium, which makes the proposed method inapplicable for producing microcapsules of water-soluble preparations in water-soluble polymers.

The technical task is to simplify and accelerate the process of obtaining nanocapsules, reduce losses in obtaining nanocapsules (increase in yield by mass).

The solution of the technical problem is achieved by the method of producing betulin nanocapsules, characterized in that gellan gum is used as the shell of the nanocapsules, and betulin is used as the core in the preparation of nanocapsules by non-solvent deposition using hexane as a precipitant.

A distinctive feature of the proposed method is the preparation of nanocapsules by non-solvent precipitation using hexane as a precipitant, as well as the use of gellan gum as a particle shell and betulin as a core.

The result of the proposed method is the preparation of betulin nanocapsules in gellan gum.

Figure 1 shows the particle size distribution in the sample of betulin nanocapsules in gellan gum (core: shell ratio 1: 3).

EXAMPLE 1

Obtaining Betulin Nanocapsules, Core: Shell Ratio 1: 3

100 mg of betulin powder is slowly added to a suspension of 300 mg of gellan gum in butanol containing 0.01 g of the preparation E472c (glycerol ester with one or two molecules of food fatty acids and one or two molecules of citric acid, and citric acid, as a tribasic, may be esterified with other glycerides and as an acid with other fatty acids. Free acid groups can be neutralized with sodium) as a surfactant with stirring at 1300 rpm. Then add 3 ml of hexane. The resulting suspension is filtered and dried at room temperature.

Obtained 0.4 g of nanocapsule powder. The yield was 100%.

EXAMPLE 2

Obtaining Betulin Nanocapsules, Core: Shell Ratio 1: 1

100 mg of betulin powder is slowly added to a suspension of 100 mg of gellan gum in butanol containing 0.01 g of the preparation E472 s with stirring at 1300 rpm. Then add 3 ml of hexane. The resulting suspension is filtered and dried at room temperature.

Obtained 0.2 g of nanocapsule powder. The yield was 100%.

EXAMPLE 3

Sizing nanocapsules by NTA method

The measurements were carried out on a Nanosight LM0 multiparameter nanoparticle analyzer manufactured by Nanosight Ltd (Great Britain) in the HS-BF configuration (Andor Luca high-sensitivity video camera, 405 nm semiconductor laser with a power of 45 mW). The device is based on the method of analysis of trajectories of nanoparticles (Nanoparticle Tracking Analysis, NTA), described in ASTM E2834.

The optimal dilution for dilution was 1: 100. For the measurement, the device parameters were selected: Camera Level = 16, Detection Threshold = 10 (multi), Min Track Length: Auto, Min Expected Size: Auto. duration of a single measurement of 215s, the use of a syringe pump.

Claims (1)

A method of producing betulin nanocapsules, characterized in that 100 mg of betulin powder is slowly added to a suspension of 100 mg or 300 mg of gellan gum in butanol in the presence of 0.01 g of the preparation E472c with stirring at 1300 rpm, then 3 ml of hexane are poured, the suspension is filtered off and dried at room temperature.

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