RU2602687C1 - Method for producing preparation for stimulation of non-specific body resistance, prevention of diseases of young farm animals - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: invention refers to veterinary medicine and aims for increasing of non-specific body resistance of young farm animals for the prevention and therapy of respiratory diseases and digestive disorders and implementation of productive capacity. Mixed 90 wt 0.2-0.3 % of agar suspension and 2.5 wt% of concentrated purified polysaccharide complex of yeast cells. To the obtained mixture with constant stirring added 3.5 wt% of benzimidazole derivative (-)2,3,5,6-tetrahydro-6-phenylimidazo[2,1-b]-thiazole hydrochloride, 5.0 million units of the antibiotic macrolides - erythromycin and 0.2 wt% of formalin, volume is adjusted to 100 wt%.

EFFECT: presented invention is highly effective for the normalization of non-specific resistance and immunogenesis, prevention and therapy of respiratory diseases and digestive disorders of young farm animals, activation of growth and development.

1 cl, 4 tbl, 4 ex

Description

The invention relates to the field of biotechnology and veterinary medicine, and in particular to methods for producing preparations for stimulating nonspecific resistance of an organism and preventing diseases of young farm animals.

A known method of obtaining a biologically active preparation from yeast (SU 1808331 A1, A61K 35/72, 04/15/93) in the form of a purified polysaccharide complex with immunostimulating activity. Immobilization of polysaccharides of yeast cells in agar gel (TU 10.07.236-91 for the production of Dostim) or in a 10-14% aqueous salt solution of polyvinylpyrrolidone (RU 2137480 C1, A61K 31/79, 35/72, 09/20/99) increases the immunostimulating activity of drugs.

The closest analogue (prototype) to the problem being solved is a method for producing a preparation for increasing nonspecific resistance and immunogenesis of an animal organism (PS-2), comprising mixing 0.2-0.3% agar suspension and a concentrate of purified polysaccharide complex of yeast cells. To the resulting mixture, (-) 2,3,5,6-tetrahydro-6-phenylimidazo [2,1-b] thiazole hydrochloride and formalin are added with constant stirring, the volume is adjusted with distilled water to 100 wt. h. The drug has immunostimulating activity (RU 2332214 C1, A61K 31/429, 31/115, A61K 36/06, A61K 36/02, A61P 37/04, 08/27/2008).

The disadvantages of the drug include the lack of antibacterial activity to animal pathogens.

The invention is directed to the stimulation of nonspecific resistance of the body and the prevention of diseases of young farm animals, expanding the range of products both to increase the activity of cellular and humoral factors of nonspecific resistance and specific immunogenesis of the body, and to increase antibacterial activity against pathogens of young animals.

The technical result consists in mixing 90 wt. including 0.2-0.3% suspension of agar and 2.5 wt. including concentrate purified polysaccharide complex of yeast cells. To the resulting mixture with constant stirring, add 3.5 wt. including benzimidazole derivative (-) 2,3,5,6-tetrahydro-6-phenylimidazo [2,1-b] thiazole hydrochloride, 5.0 million units of macrolide antibiotic - erythromycin - [3R- (3R * , 4S *, 5S *, 6R *, 7R *, 9R *, 11R *, 12R *, 13S *, 14R *)] - 4 [(2,6-Dideoxy-3-O-methyl-3-0-methyl alpha-b-ribohexopyranosyl) oxy] -14-ethyl-7,12,13-trihydroxy-3,5,7,9,11,13-hexamethyl-6 - [[3,4,6-tridesoxy-3- (dimethylamino) -beta-D-xylohexopyranosyl) oxy] oxacyclotetradecane-2,10-dione and 0.2 wt. including formalin, the volume is adjusted with distilled water to 100 wt. h. The resulting preparation is poured into 50-100 ml bottles, after sterilization it is used to stimulate the nonspecific resistance of the body and prevent diseases of young farm animals.

The difference of the proposed solution from the known analogue is that when the antibiotic erythromycin is administered together with the polysaccharide complex and (-) 2,3,5,6-tetrahydro-6-phenylimidazo [2,1-b] thiazole hydrochloride, their effect is enhanced on factors of nonspecific resistance of the body, phagocytosis is activated and the spectrum of bactericidal activity against pathogens of young farm animals is significantly expanded.

To implement the method used the following substances:

1) microbiological agar GOST 17206;

2) the concentrate of the purified polysaccharide complex of yeast SU 1808331;

3) (-) 2,3,5,6-tetrahydro-6-phenylimidazo [2.1-b] thiazole hydrochloride TU 9333-024;

4) erythromycin;

5) formalin GOST 1625;

6) distilled water GOST 6709.

The method is as follows:

Example 1. 90 wt. including 0.2% suspension of agar was mixed with 2.5 wt. including concentrate purified polysaccharide complex of yeast. To the resulting mixture, 3.5 wt. including (-) 2,3,5,6-tetrahydro-6-phenylimidazo [2,1-b] thiazole hydrochloride, 5.0 million units of an antibiotic erythromycin and 0.2 wt. including formalin, the volume was adjusted with distilled water to 100 wt. h. The obtained preparation after sterilization was used to stimulate the nonspecific resistance of the organism and to prevent diseases of young farm animals.

Example 2. 90 wt. including 0.3% suspension of agar was mixed with 2.5 wt. including concentrate purified polysaccharide complex of yeast. To the resulting mixture, 3.5 wt. including (-) 2,3,5,6-tetrahydro-6-phenylimidazo [2,1-b] thiazole hydrochloride, 5.0 million units of an antibiotic erythromycin and 0.2 wt. including formalin, the volume was adjusted with distilled water to 100 wt. h. The obtained preparation after sterilization was used to stimulate the nonspecific resistance of the organism and to prevent diseases of young farm animals.

Example 3. The objects of research were pigs of large white breed. In the scientific and economic experiment, four groups of newborn piglets (control, 1st, 2nd and 3rd experimental) were selected according to the principle of analogue pairs taking into account the clinical and physiological state, age and live weight of 10 animals in each group.

Pigs of the 1st experimental group were injected intramuscularly with PS-2 (prototype) at a dose of 0.2 ml per head three times at 1-, 4- and 7-day-olds, the 2nd experimental group - the drug according to the invention (example 1), 3 -th experimental group — the preparation according to the invention (example 2) at the indicated dose and time (experimental options), the fourth group was the control.

Indicators of nonspecific resistance of the organism of piglets were determined at the age of 10, 20 and 30 days. The results of these studies are given in table. one.

It was found that the proposed drug in examples 1 and 2 improves the indicators of nonspecific resistance of the organism of piglets at the age of 10 days: phagocytic activity - by 0.8 and 0.6%, lysozyme activity - by 1.4 and 1.2%, bactericidal activity - by 2.2 and 2.0%; at the age of 20 days - by 5.0 and 4.6%, 2.2 and 2.0%, 3.0 and 2.6; at the age of 30 days - by 5.0 and 5.4%, 3.6 and 3.8%, 2.6 and 2.8% (P <0.05-0.001).

Indicators of morbidity and safety of piglets are presented in table. 2.

In the control group, 8 piglets or 80.0% fell ill, of which 1 piglet fell and 7 recovered. Piglets showed signs of acute inflammation of the gastrointestinal tract (diarrhea). The duration of the disease was 4.6 ± 0.40 days. The occurrence of the disease in piglets occurred as a result of a decrease in the protective properties of the organism, in which the activity of the conditionally pathogenic microflora increases, which leads to an increased risk of developing the disease, in particular escherichiosis.

The incidence of piglets in the 1st, 2nd and 3rd experimental groups was 40, 40 and 30%, respectively, which is 2.0, 2.0 and 2.7 times less than in the control. The disease in them also proceeded with signs of inflammation of the intestinal tract (diarrhea), but the degree of manifestation of clinical signs and the duration of the disease was 1.64, 1.77 and 2.09 times less than in the control. The safety of piglets in the experimental groups was 100%.

Thus, under the influence of the developed drug based on the polysaccharide complex of yeast cells and the erythromycin antibiotic in piglets, cellular and humoral factors of nonspecific resistance of the organism became more active, and the number and duration of digestive diseases decreased.

Example 4. The test drug to study the immunostimulating properties and prophylactic efficacy was performed on calves selected by the principle of pair-analogues at 1 day of age. Four groups of calves were selected (control, 1st, 2nd and 3rd experimental), 10 animals in each group. The calves of the 1st experimental group were injected intramuscularly with PS-2 (prototype) at a dose of 0.1 ml / kg of live weight three times at 1-, 4- and 7-day-old age, the 2nd experimental group - the preparation according to the invention (example 1) , 3rd experimental group - the preparation according to the invention (example 2) at the indicated dose and time (experimental options), the fourth group was the control.

Indicators of nonspecific resistance of the calf organism were determined at the age of 14, 30 and 60 days. The results of these studies are presented in table. 3.

It was found that the proposed drug according to examples 1 and 2 improves the hematological parameters of nonspecific resistance of the calf’s body at the age of 14 days: phagocytic activity by 1.4 and 1.0%, lysozyme activity by 2.6 and 2.4%, bactericidal activity - by 5.4 and 5.2%; at the age of 30 days - by 2.8 and 2.4%, 4.6 and 4.2%, 7.0 and 6.8%; at the age of 60 days - by 5.2 and 5.6%, 4.4 and 4.6%, 7.2 and 7.4% (P <0.05-0.001).

Before reaching the age of 180 days, the incidence and safety of calves was monitored, the research results are given in table. four.

In the control group, 5 calves fell ill or 50.0%. In calves, signs of acute inflammation of the gastrointestinal tract (diarrhea), signs of respiratory disease (bronchitis) were recorded. The average duration of illness was 6.2 ± 0.66 days. The occurrence of diseases in calves occurred as a result of a decrease in the protective properties of the body, as a result of which the adaptive ability of the body is significantly reduced and the activity of conditionally pathogenic microflora increases, which leads to an increased risk of developing diseases.

The incidence of calves in the 1st, 2nd and 3rd experimental groups was 30, 20 and 20%, respectively, which is 1.7, 2.5 and 2.5 times less than in the control. Diseases in calves proceeded with signs of inflammation of the gastrointestinal tract, with signs of respiratory disease, but the degree of manifestation of clinical signs and the duration of the disease was 1.48, 1.82 and 2.07 times less than in the control. The safety of calves in the experimental groups was 100%.

Thus, under the influence of the developed drug based on the polysaccharide complex of yeast cells and the erythromycin antibiotic in piglets and calves, cellular and humoral factors of nonspecific resistance of the body are activated, the number and duration of respiratory and digestive diseases are reduced.

Claims (1)

A method of obtaining a drug for stimulating nonspecific resistance of the body and preventing diseases of young farm animals, comprising mixing 90 wt. including 0.2-0.3% suspension of agar, 2.5 wt. including concentrate purified polysaccharide complex of yeast, 3.5 wt. including (-) 2,3,5,6-tetrahydro-6-phenylimidazo [2,1-b] thiazole hydrochloride and 0.2 wt. including formalin, when mixing is additionally introduced 5.0 million units of the antibiotic erythromycin and the volume of the drug is adjusted with distilled water to 100 wt. hours