CN104705495A - Special traditional Chinese medicine polysaccharide feed additive for pets - Google Patents

Special traditional Chinese medicine polysaccharide feed additive for pets Download PDF

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Publication number
CN104705495A
CN104705495A CN201510146362.6A CN201510146362A CN104705495A CN 104705495 A CN104705495 A CN 104705495A CN 201510146362 A CN201510146362 A CN 201510146362A CN 104705495 A CN104705495 A CN 104705495A
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parts
polysaccharide
vitamin
pets
pet
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宗敏玲
付仲襄
付宁晨
徐晓曦
刘志辉
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To Be Grown Up In Jiangxi Pharmaceutcal Corp Ltd
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To Be Grown Up In Jiangxi Pharmaceutcal Corp Ltd
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Abstract

The invention provides a special traditional Chinese medicine polysaccharide feed additive for pets. The formula is selected from astragalus polysaccharide, polysaccharides from radix codonopsis, glycyrrhiza polysaccharide and vitamins; plant polysaccharide is one of the main active components of traditional Chinese medicine; vitamins are necessary substances for maintaining the normal growth and metabolism of animals; the combination of plant polysaccharide and vitamins has the effect of enhancing the anti-stress of pets in the high-temperature and low-temperature severe environments; by virtue of the combined utilization of rosmarinic acid, sodium tripolyphosphate, titanium dioxide, sorbitol, microcrystalline cellulose and vitexin, the anti-stress effect of astragalus polysaccharide, polysaccharides from radix codonopsis, glycyrrhiza polysaccharide and vitamins on pets can be improved, the survival rate of pets in severe environments can be enhanced, meanwhile the immune response capability of pets can be reduced, and the immune antibody level of pseudo rabies vaccines can be reduced. By virtue of a great amount of explorations, the problem of reduction of immune response capability of pets can be solved by combining the special preparation technology, and the immune antibody level of pseudo rabies vaccines can be improved.

Description

The herbal polysaccharide feed addictive that a kind of pet is special
Technical field
The present invention relates to a kind of herbal polysaccharide feed addictive, particularly relate to the herbal polysaccharide feed addictive that a kind of pet is special.
Background technology
Stress refer to a kind of general nonspecific reaction caused by various different stress factor (as cold, warm, living environment change etc.), it is a kind of Adaptive mechanism of living organism.But excessively strong stress reaction can cause pet growth retardation, reproductive capability obstacle, production performance to decline, even cause death of pet.Modern study shows, Chinese herbal medicine has good anti-stress effect, and safety has no side effect.Therefore, the anti allergic reaction utilizing Chinese herbal medicine to alleviate pet has vast potential for future development.
Pseudoabies is sick also known as AujeszkyShi, and being the multiple pet such as dog, pig, sheep that caused by herpetoviridae herpesviral subfamilies Simplex Virus Type I and domestic animal to itch and a kind of acute infectious disease that encephalomyelitis is cardinal symptom with heating, very.Pseudorabies virus under field conditions (factors) can the animal such as infected pigs, ox, sheep, dog, cat, rabbit, mouse, wild boar, ermine, bear, fox.
Pseudorabies virus infects general atypism pathology.Observe and mainly see that kidney has tip-like blutpunkte, Other Meat illness in eye becomes not obvious.Visible catarrhal gastritis in various degree and enteritis, when central nervous system symptom is obvious, meninx is obviously congested, myelencephalon hypervolia, the substantial viscera such as liver, spleen Chang Kejian canescence necrotic lesion, stethemia, oedema and downright bad point.Solubility phase diagram placentitis can be developed into after intra-uterine infection.
Summary of the invention
The object of the present invention is to provide the herbal polysaccharide feed addictive that a kind of pet is special, the anti-stress effect of pet to high temperature, these adverse circumstances of low temperature can be improved, strengthen the immune response of pet, improve pet pseudo-rabies vaccine immune antiboidy level.
The herbal polysaccharide feed addictive that pet is special, comprises the raw material of following weight portion:
Astragalus polyose 245-375 part, Codonopsis pilosula polysaccharide 285-375 part, licorice polysaccharide 125-245 part, vitamin C 5-10 part, vitamin E 5-10 part, Rosmarinic acid 10-18 part, sodium phosphate trimer 5-10 part, titanium dioxide 3-8 part, D-sorbite 2-5 part, microcrystalline cellulose 3-10 part, isovitexin 1-3 part;
The preparation method of astragalus polyose is: get the water soaking 12h that Radix Astragali medicine materical crude slice adds 8 times of Radix Astragali prepared slice qualities, heating and refluxing extraction 2 times, each 2h, merges and leaches solution, concentrated, add again volume fraction be 75% ethanolic solution carry out alcohol precipitation, leave standstill 6h, filter, carry out spraying dry again, spraying dry EAT is 175 DEG C, and leaving air temp is 115 DEG C, obtains astragalus polyose powder after drying;
The preparation method of Codonopsis pilosula polysaccharide is: get the water soaking 12h that Radix Codonopsis medicine materical crude slice adds 8 times of Radix Codonopsis prepared slice qualities, heating and refluxing extraction 2 times, each 2h, merges and leaches solution, concentrated, add again volume fraction be 75% ethanolic solution carry out alcohol precipitation, leave standstill 6h, filter, carry out spraying dry again, spraying dry EAT is 175 DEG C, and leaving air temp is 115 DEG C, obtains Codonopsis pilosula polysaccharide powder after drying;
The preparation method of licorice polysaccharide is: extracting liquorice medicine materical crude slice adds the water soaking 12h of 8 times of licorice piece quality, heating and refluxing extraction 2 times, each 2h, merges and leaches solution, concentrated, add again volume fraction be 75% ethanolic solution carry out alcohol precipitation, leave standstill 6h, filter, carry out spraying dry again, spraying dry EAT is 175 DEG C, and leaving air temp is 115 DEG C, obtains licorice polysaccharide powder after drying;
Preparation method of the present invention is as follows:
1) astragalus polyose, Codonopsis pilosula polysaccharide, titanium dioxide mixing are placed in the steam of 90-100 DEG C and process 1-3min, be then cooled to 20-25 DEG C rapidly, leave standstill 10-15h, for subsequent use;
2) licorice polysaccharide is heated to 50-60 DEG C, then adds D-sorbite, microcrystalline cellulose, sodium phosphate trimer, stir evenly, be cooled to 20-25 DEG C, for subsequent use;
3) by vitamin C, vitamin E, Rosmarinic acid, isovitexin mixing, for subsequent use;
4) by step 1), 2), 3) mixture that obtains mixes.
The present invention's formula has selected astragalus polyose, Codonopsis pilosula polysaccharide, licorice polysaccharide, vitamin, plant polyose is one of main active of Chinese medicine, vitamin maintains animal normal growth and the requisite material of metabolism, and plant polyose and vitamin share to have and strengthens the anti-stress effect of pet in high temperature, these adverse circumstances of low temperature.And Rosmarinic acid, sodium phosphate trimer, titanium dioxide, D-sorbite, microcrystalline cellulose, isovitexin conbined usage can improve astragalus polyose, Codonopsis pilosula polysaccharide, licorice polysaccharide, vitamin to the anti-stress effect of pet, improve the survival rate of pet in adverse circumstances, but pet immune response ability can be reduced simultaneously, reduce pseudo-rabies vaccine immune antiboidy level.We are through a large amount of exploration discovery, in conjunction with the problem that the distinctive preparation technology of the present invention can solve the reduction of pet immune response ability, improve pseudo-rabies vaccine immune antiboidy level.
Advantage of the present invention is as follows:
(1) pet strengthens the anti-stress effect of high temperature, these adverse circumstances of low temperature, and the survival rate in adverse circumstances increases;
(2) immune response of pet can be strengthened, improve pet pseudo-rabies vaccine immune antiboidy level;
(3) additive of the present invention take plant polyose as main component, safety, non-environmental-pollution, without hazard residue, has complied with the development trend of feed addictive.
Specific embodiment
Embodiment 1: the herbal polysaccharide feed addictive that a kind of pet is special, comprises the raw material of following weight portion:
Astragalus polyose 300 parts, Codonopsis pilosula polysaccharide 320 parts, licorice polysaccharide 185 parts, vitamin C 8 parts, vitamin E 7 parts, Rosmarinic acid 14 parts, sodium phosphate trimer 8 parts, titanium dioxide 5 parts, D-sorbite 3 parts, microcrystalline cellulose 6 parts, isovitexin 2 parts;
The preparation method of astragalus polyose is: get the water soaking 12h that Radix Astragali medicine materical crude slice adds 8 times of Radix Astragali prepared slice qualities, heating and refluxing extraction 2 times, each 2h, merges and leaches solution, concentrated, add again volume fraction be 75% ethanolic solution carry out alcohol precipitation, leave standstill 6h, filter, carry out spraying dry again, spraying dry EAT is 175 DEG C, and leaving air temp is 115 DEG C, obtains astragalus polyose powder after drying;
The preparation method of Codonopsis pilosula polysaccharide is: get the water soaking 12h that Radix Codonopsis medicine materical crude slice adds 8 times of Radix Codonopsis prepared slice qualities, heating and refluxing extraction 2 times, each 2h, merges and leaches solution, concentrated, add again volume fraction be 75% ethanolic solution carry out alcohol precipitation, leave standstill 6h, filter, carry out spraying dry again, spraying dry EAT is 175 DEG C, and leaving air temp is 115 DEG C, obtains Codonopsis pilosula polysaccharide powder after drying;
The preparation method of licorice polysaccharide is: extracting liquorice medicine materical crude slice adds the water soaking 12h of 8 times of licorice piece quality, heating and refluxing extraction 2 times, each 2h, merges and leaches solution, concentrated, add again volume fraction be 75% ethanolic solution carry out alcohol precipitation, leave standstill 6h, filter, carry out spraying dry again, spraying dry EAT is 175 DEG C, and leaving air temp is 115 DEG C, obtains licorice polysaccharide powder after drying;
Its preparation method is as follows:
1) astragalus polyose, Codonopsis pilosula polysaccharide, titanium dioxide mixing are placed in the steam of 95 DEG C and process 2min, be then cooled to rapidly 22 DEG C, leave standstill 12h, for subsequent use;
2) licorice polysaccharide is heated to 55 DEG C, then adds D-sorbite, microcrystalline cellulose, sodium phosphate trimer, stir evenly, be cooled to 22 DEG C, for subsequent use;
3) by vitamin C, vitamin E, Rosmarinic acid, isovitexin mixing, for subsequent use;
4) by step 1), 2), 3) mixture that obtains mixes.
Embodiment 2: the herbal polysaccharide feed addictive that a kind of pet is special, comprises the raw material of following weight portion:
Astragalus polyose 245 parts, Codonopsis pilosula polysaccharide 285 parts, licorice polysaccharide 125 parts, vitamin C 5 parts, vitamin E 5 parts, Rosmarinic acid 10 parts, sodium phosphate trimer 5 parts, titanium dioxide 3 parts, D-sorbite 2 parts, microcrystalline cellulose 3 parts, isovitexin 1 part;
Its preparation method is as follows:
1) astragalus polyose, Codonopsis pilosula polysaccharide, titanium dioxide mixing are placed in the steam of 90 DEG C and process 1min, be then cooled to rapidly 20 DEG C, leave standstill 10h, for subsequent use;
2) licorice polysaccharide is heated to 50 DEG C, then adds D-sorbite, microcrystalline cellulose, sodium phosphate trimer, stir evenly, be cooled to 20 DEG C, for subsequent use;
3) by vitamin C, vitamin E, Rosmarinic acid, isovitexin mixing, for subsequent use;
4) by step 1), 2), 3) mixture that obtains mixes.
All the other are with embodiment 1.
Embodiment 3: the herbal polysaccharide feed addictive that a kind of pet is special, comprises the raw material of following weight portion:
Astragalus polyose 375 parts, Codonopsis pilosula polysaccharide 375 parts, licorice polysaccharide 245 parts, vitamin C 10 parts, vitamin E 10 parts, Rosmarinic acid 18 parts, sodium phosphate trimer 10 parts, titanium dioxide 8 parts, D-sorbite 5 parts, microcrystalline cellulose 10 parts, isovitexin 3 parts;
Its preparation method is as follows:
1) astragalus polyose, Codonopsis pilosula polysaccharide, titanium dioxide mixing are placed in the steam of 100 DEG C and process 3min, be then cooled to rapidly 25 DEG C, leave standstill 15h, for subsequent use;
2) licorice polysaccharide is heated to 60 DEG C, then adds D-sorbite, microcrystalline cellulose, sodium phosphate trimer, stir evenly, be cooled to 25 DEG C, for subsequent use;
3) by vitamin C, vitamin E, Rosmarinic acid, isovitexin mixing, for subsequent use;
4) by step 1), 2), 3) mixture that obtains mixes.
All the other are with embodiment 1.
Embodiment 4: the herbal polysaccharide feed addictive that a kind of pet is special, comprises the raw material of following weight portion:
Astragalus polyose 235 parts, Codonopsis pilosula polysaccharide 385 parts, licorice polysaccharide 110 parts, vitamin C 4 parts, vitamin E 11 parts, Rosmarinic acid 9 parts, sodium phosphate trimer 12 parts, titanium dioxide 2 parts, D-sorbite 1 part, microcrystalline cellulose 11 parts, isovitexin 4 parts;
Its preparation method is as follows:
1) astragalus polyose, Rosmarinic acid, titanium dioxide mixing are placed in the steam of 85 DEG C and process 4min, be then cooled to rapidly 15 DEG C, leave standstill 8h, for subsequent use;
2) licorice polysaccharide is heated to 45 DEG C, then adds vitamin E, D-sorbite, sodium phosphate trimer, stir evenly, be cooled to 30 DEG C, for subsequent use;
3) by vitamin C, Codonopsis pilosula polysaccharide, microcrystalline cellulose, isovitexin mixing, for subsequent use;
4) by step 1), 2), 3) mixture that obtains mixes.
All the other are with embodiment 1.
Embodiment 5: the herbal polysaccharide feed addictive that a kind of pet is special, its preparation method is as follows:
1) vitamin E, Rosmarinic acid, sodium phosphate trimer, isovitexin mixing are placed in the steam of 110 DEG C and process 5min, be then cooled to rapidly 30 DEG C, leave standstill 18h, for subsequent use;
2) licorice polysaccharide is heated to 65 DEG C, then add D-sorbite, titanium dioxide microcrystalline cellulose, stir evenly, be cooled to 30 DEG C, for subsequent use;
3) by astragalus polyose, Codonopsis pilosula polysaccharide, vitamin C mixing, for subsequent use;
4) by step 1), 2), 3) mixture that obtains mixes.
All the other are with embodiment 1.
Embodiment 6: the herbal polysaccharide feed addictive that a kind of pet is special, comprises the raw material of following weight portion:
Astragalus polyose 300 parts, Codonopsis pilosula polysaccharide 320 parts, licorice polysaccharide 185 parts, vitamin C 8 parts, vitamin E 7 parts;
Its preparation method is: by astragalus polyose, Codonopsis pilosula polysaccharide, licorice polysaccharide, vitamin C, vitamin E mixing.
Wherein the preparation method of astragalus polyose, Codonopsis pilosula polysaccharide, licorice polysaccharide is with embodiment 1.
Test example 1: resisting stress is tested
Choose healthy, that body weight is close Hu Liyani pig 140, be divided into 7 groups at random, be divided into test group and blank group, often organize 20.Add the feed addictive that embodiment 1-6 is obtained in the daily ration of test group Hu Liyani pig respectively, addition is 1% of daily ration weight, does not add arbitrary feed addictive that embodiment 1-6 is obtained, all the other same test group in the daily ration of blank group Hu Liyani pig.Experimental period is 15d, period Hu Liyani pig free choice feeding and drinking-water.Being divided into Liang Ge group at random by often organizing 20 Hu Liyani pigs during 15d, each group 10, being placed in the low temperature environment of-19 to-21 DEG C and the hot environment of 41 to 42 DEG C respectively, observe 50-60min, record Hu Liyani pig survival number.The results are shown in Table 1.
Table 1 Hu Liyani pig resisting stress result of the test
Test example 2: pseudo-rabies vaccine antibody horizontal test
Choose healthy, that body weight is close safe enlightening dog 35, be divided into 7 groups at random, be divided into test group and blank group, often organize 5.Add the feed addictive that embodiment 1-6 is obtained in test group daily ration respectively, addition is 2% of daily ration weight, does not add the obtained arbitrary feed addictive of embodiment 1-6, all the other same test group in the daily ration of the safe enlightening dog of blank group.Experimental period is 21d, period safe enlightening dog free choice feeding and drinking-water.Within 7th day, inoculate pseudo-rabies vaccine in test, then test the 14th and 21 days, each group of safe enlightening dog is taken a blood sample by vena cava anterior, separation of serum after standing 30 min.Enzyme-linked immunosorbent assay (ELISA) is adopted to detect pseudoabies antibody.Record the sample/negative control ratio (S/N) often organized, the results are shown in Table 2.
Criterion: S/N > 0.7, sample should be judged to be PRV gpI negative antibody;
0.6 < S/N≤0.7, sample should redeterminate;
S/N≤0.6, sample should be judged to be PRV gpI antibody positive; S/N value less explanation antibody horizontal is higher, and immune effect is better.
Table 2 pseudo-Rabies virus antibody level result
From above-mentioned test example, the survival rate of pet in high temperature, these adverse circumstances of low temperature of the experimental group that embodiment 1-3 is corresponding is higher, Rosmarinic acid, sodium phosphate trimer, titanium dioxide, D-sorbite, microcrystalline cellulose, isovitexin is not added higher than embodiment 6(, and do not adopt preparation technology of the present invention) corresponding experimental group, especially with the effect of embodiment 1 for the best.Embodiment 5(formulation parameter is in the scope of technical solution of the present invention, but not adopting the technique in technical solution of the present invention) survival rate of pet is corresponding with embodiment 1 in corresponding experimental group experimental group effect is close, but pseudo-Rabies virus antibody level is far away not as good as the experimental group effect of embodiment 1 correspondence, not even as the effect of blank group, illustrate that technique of the present invention can solve in formula and add Rosmarinic acid, sodium phosphate trimer, titanium dioxide, D-sorbite, microcrystalline cellulose, the pet immune response ability that isovitexin brings reduces, the problem that pseudo-rabies vaccine immune antiboidy level declines.

Claims (5)

1. the herbal polysaccharide feed addictive that pet is special, is characterized in that: comprise following raw material: astragalus polyose, Codonopsis pilosula polysaccharide, licorice polysaccharide, vitamin C, vitamin E, Rosmarinic acid, sodium phosphate trimer, titanium dioxide, D-sorbite, microcrystalline cellulose, isovitexin.
2. the herbal polysaccharide feed addictive that a kind of pet as claimed in claim 1 is special, is characterized in that: the raw material comprising following weight portion:
Astragalus polyose 300 parts, Codonopsis pilosula polysaccharide 320 parts, licorice polysaccharide 185 parts, vitamin C 8 parts, vitamin E 7 parts, Rosmarinic acid 14 parts, sodium phosphate trimer 8 parts, titanium dioxide 5 parts, D-sorbite 3 parts, microcrystalline cellulose 6 parts, isovitexin 2 parts;
The preparation method of astragalus polyose is: get the water soaking 12h that Radix Astragali medicine materical crude slice adds 8 times of Radix Astragali prepared slice qualities, heating and refluxing extraction 2 times, each 2h, merges and leaches solution, concentrated, add again volume fraction be 75% ethanolic solution carry out alcohol precipitation, leave standstill 6h, filter, carry out spraying dry again, spraying dry EAT is 175 DEG C, and leaving air temp is 115 DEG C, obtains astragalus polyose powder after drying;
The preparation method of Codonopsis pilosula polysaccharide is: get the water soaking 12h that Radix Codonopsis medicine materical crude slice adds 8 times of Radix Codonopsis prepared slice qualities, heating and refluxing extraction 2 times, each 2h, merges and leaches solution, concentrated, add again volume fraction be 75% ethanolic solution carry out alcohol precipitation, leave standstill 6h, filter, carry out spraying dry again, spraying dry EAT is 175 DEG C, and leaving air temp is 115 DEG C, obtains Codonopsis pilosula polysaccharide powder after drying;
The preparation method of licorice polysaccharide is: extracting liquorice medicine materical crude slice adds the water soaking 12h of 8 times of licorice piece quality, heating and refluxing extraction 2 times, each 2h, merges and leaches solution, concentrated, add again volume fraction be 75% ethanolic solution carry out alcohol precipitation, leave standstill 6h, filter, carry out spraying dry again, spraying dry EAT is 175 DEG C, and leaving air temp is 115 DEG C, obtains licorice polysaccharide powder after drying.
3. the herbal polysaccharide feed addictive that a kind of pet as claimed in claim 2 is special, is characterized in that: the raw material comprising following weight portion: astragalus polyose 300 parts, Codonopsis pilosula polysaccharide 320 parts, licorice polysaccharide 185 parts, vitamin C 8 parts, vitamin E 7 parts, Rosmarinic acid 14 parts, sodium phosphate trimer 8 parts, titanium dioxide 5 parts, D-sorbite 3 parts, microcrystalline cellulose 6 parts, isovitexin 2 parts.
4. the herbal polysaccharide feed addictive that a kind of pet as claimed in claim 2 or claim 3 is special, is characterized in that: preparation method is as follows:
1) astragalus polyose, Codonopsis pilosula polysaccharide, titanium dioxide mixing are placed in the steam of 90-100 DEG C and process 1-3min, be then cooled to 20-25 DEG C rapidly, leave standstill 10-15h, for subsequent use;
2) licorice polysaccharide is heated to 50-60 DEG C, then adds D-sorbite, microcrystalline cellulose, sodium phosphate trimer, stir evenly, be cooled to 20-25 DEG C, for subsequent use;
3) by vitamin C, vitamin E, Rosmarinic acid, isovitexin mixing, for subsequent use;
4) by step 1), 2), 3) mixture that obtains mixes.
5. the herbal polysaccharide feed addictive that a kind of pet as claimed in claim 4 is special, is characterized in that: preparation method is as follows:
1) astragalus polyose, Codonopsis pilosula polysaccharide, titanium dioxide mixing are placed in the steam of 95 DEG C and process 2min, be then cooled to rapidly 22 DEG C, leave standstill 12h, for subsequent use;
2) licorice polysaccharide is heated to 55 DEG C, then adds D-sorbite, microcrystalline cellulose, sodium phosphate trimer, stir evenly, be cooled to 22 DEG C, for subsequent use;
3) by vitamin C, vitamin E, Rosmarinic acid, isovitexin mixing, for subsequent use;
4) by step 1), 2), 3) mixture that obtains mixes.
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RU2622765C2 (en) * 2015-08-31 2017-06-19 Федеральное государственное бюджетное образовательное учреждение высшего образования "Чувашская государственная сельскохозяйственная академия" (ФГБОУ ВО Чувашская ГСХА) Method of producing preparation for implementation of reproductive properties of cows and productive potential of calves
RU2602687C1 (en) * 2015-09-15 2016-11-20 Федеральное государственное бюджетное образовательное учреждение высшего образования "Чувашская государственная сельскохозяйственная академия" (ФГБОУ ВО Чувашская ГСХА) Method for producing preparation for stimulation of non-specific body resistance, prevention of diseases of young farm animals
RU2622981C2 (en) * 2015-09-15 2017-06-21 Федеральное государственное бюджетное образовательное учреждение высшего образования "Чувашская государственная сельскохозяйственная академия" (ФГБОУ ВО Чувашская ГСХА) Method of producing preparation for implementation of biological potential of farm animals
CN106804485A (en) * 2017-03-21 2017-06-09 中国水产科学研究院淡水渔业研究中心 A kind of method that knife long-tailed anchovy small size pond temporarily supports resisting stress
CN114984035A (en) * 2022-05-27 2022-09-02 扬州大学 Application of glycyrrhiza polysaccharide in preparation of preparation for resisting porcine pseudorabies virus infection
RU2805330C1 (en) * 2022-12-13 2023-10-16 Федеральное государственное бюджетное образовательное учреждение высшего образования "Чувашский государственный аграрный университет" Method of producing drug for increasing non-specific resistance of the organism, prevention and treatment of diseases in farm poultry
RU2805652C1 (en) * 2022-12-19 2023-10-23 Федеральное государственное бюджетное образовательное учреждение высшего образования "Чувашский государственный аграрный университет" Method of producing a drug for activation of non-specific resistance and increasing the efficiency of specific prevention of animal diseases

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Application publication date: 20150617