CN106804485A - A kind of method that knife long-tailed anchovy small size pond temporarily supports resisting stress - Google Patents
A kind of method that knife long-tailed anchovy small size pond temporarily supports resisting stress Download PDFInfo
- Publication number
- CN106804485A CN106804485A CN201710168651.5A CN201710168651A CN106804485A CN 106804485 A CN106804485 A CN 106804485A CN 201710168651 A CN201710168651 A CN 201710168651A CN 106804485 A CN106804485 A CN 106804485A
- Authority
- CN
- China
- Prior art keywords
- open
- temporarily
- top receptacle
- tailed anchovy
- small size
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241001430403 Coilia grayii Species 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000009395 breeding Methods 0.000 claims abstract description 6
- 230000001488 breeding effect Effects 0.000 claims abstract description 6
- 239000012814 acoustic material Substances 0.000 claims abstract description 4
- 238000013019 agitation Methods 0.000 claims abstract description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 4
- 239000001301 oxygen Substances 0.000 claims abstract description 4
- 206010063036 Spinal cord oedema Diseases 0.000 claims abstract description 3
- 230000009467 reduction Effects 0.000 claims abstract description 3
- 241001371765 Coilia ramcarti Species 0.000 claims description 5
- 238000009434 installation Methods 0.000 claims description 2
- 239000012774 insulation material Substances 0.000 claims 1
- 230000002180 anti-stress Effects 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 5
- 239000003795 chemical substances by application Substances 0.000 abstract description 4
- 230000004899 motility Effects 0.000 abstract description 4
- 238000009360 aquaculture Methods 0.000 abstract description 3
- 244000144974 aquaculture Species 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 description 7
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 6
- 241001460967 Coilia nasus Species 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 229960000890 hydrocortisone Drugs 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001125843 Trichiuridae Species 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000008288 physiological mechanism Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A kind of method that resisting stress is temporarily supported the invention discloses knife long-tailed anchovy small size pond, it is characterised in that comprise the steps：（1）Temporarily support instrument：Using open-top receptacle；（2）Except treatment of making an uproar：By above-mentioned open-top receptacle and ground-contacting position cushioning elasticity acoustic material；（3）Water body regulates and controls：Knife long-tailed anchovy breeding water is added in open-top receptacle, it is ensured that its dissolved oxygen is higher than 7.0mg/L, pH 7.0 7.6, water temperature is less than 25 DEG C, the sorbierite of the mass ratio of addition 0.6% 1.2%, by agitation fully dissolving；（4）To temporarily be supported in knife long-tailed anchovy band Spinal Cord Oedema to open-top receptacle, the open-top receptacle oral area sets shade, reduces light, and reduction stress.The present invention is simple using instrument, is the customary furnishings in aquaculture, obtains convenient.The Antistress agent for using is single component, and nontoxic, and cost is relatively low, and 6 hours small size ponds significantly, can temporarily be formed motility rate and bring up to more than 96% by anti-stress effect.
Description
Technical field
The invention belongs to technical field of aquaculture, it is related to a kind of resisting stress method in small size pond during temporary foster knife long-tailed anchovy.
Background technology
Coilia ectenes in Yangtze River (Coilia nasus), is commonly called as hairtail, is rare rare breeding migration fish in the Changjiang river, with
It is fresh, tender, beautiful and well-known, the good reputation first of " the Changjiang river three delicacies " is enjoyed, it is China's economic species of important famous-particular-excellent.However, knife long-tailed anchovy
Stress reaction is strong, has " water outlet is i.e. dead " to say, multiple links need temporarily to be supported in small size pond in breeding process, and this
Easily triggering in small size pond stress be dead, causes serious economic loss.Therefore, it is necessary to it is small to develop a kind of raising Coilia ectenes in Yangtze River
The method that water body temporarily forms motility rate, reduces the death rate of the process, reduces economic loss.
In other aquatic livestocks, anti-stress complex is generally made using B B-complex, Chinese herbal medicine and glucose etc.,
Possesses certain resisting stress effect.According to the experiment of inventor, aquatic products Antistress agent in the market is in Coilia ectenes in Yangtze River
Effect is not obvious.
The content of the invention
A kind of physiological mechanism that stress regulate and control from knife long-tailed anchovy is provided present invention aim at the defect for prior art to start with,
Effectively improve in the small size pond of the survival rate that knife long-tailed anchovy small size pond temporarily supports 6 hours resisting stress method during temporarily foster knife long-tailed anchovy.
The present invention to achieve the above object, is adopted the following technical scheme that：
A kind of method that knife long-tailed anchovy small size pond temporarily supports resisting stress, it is characterised in that comprise the steps：
(1) instrument is temporarily supported：Using open-top receptacle；
(2) except treatment of making an uproar：By above-mentioned open-top receptacle and ground-contacting position cushioning elasticity acoustic material；
(3) water body regulation and control：Knife long-tailed anchovy breeding water is added in open-top receptacle, it is ensured that its dissolved oxygen is higher than 7.0mg/L, pH7.0-
7.6, water temperature is less than 25 DEG C, adds the sorbierite of 0.6%-1.2% mass ratioes, by agitation fully dissolving；
(4) will temporarily be supported in knife long-tailed anchovy band Spinal Cord Oedema to open-top receptacle, the open-top receptacle oral area sets shade, reduces light
Line, reduction stress.
Preferably：The open-top receptacle volume is 1L-1000L.
The elastic acoustic material is sponge of the thickness more than 10cm.
The solar protection devices is double-layered sunshade net.
Installation of water is the 2/3 of open-top receptacle in the step (3).
The temporarily foster density of knife tapertail anchovy is 0.5-2.5g/L in the step (4).
Compared with the prior art, had the advantages that using the invention：
1. the invention is simple using instrument, is the customary furnishings in aquaculture, obtains convenient.
2. the Antistress agent for using is single component, and nontoxic, and cost is relatively low, and anti-stress effect significantly, can be by 6
Hour small size pond temporarily forms motility rate and brings up to more than 96%.
Brief description of the drawings
Fig. 1 is control group and the change compares figure using the inventive method knife tapertail anchovy group blood plasma cortisol.
Fig. 2 is control group and the change compares figure using the inventive method knife tapertail anchovy group plasma glucose.
Fig. 3 is using the inventive method knife tapertail anchovy GLO-1 variation diagram.
Fig. 4 is that GLO-1 contrasts electrophoretogram.
Specific embodiment
With reference to concrete case, the present invention is described in further detail, but embodiments of the present invention are not limited to
This.In the case of not specified (NS), the reagent of present invention use, apparatus and method are the art examination routinely purchased in market
Agent, equipment and conventional use of method.
1. the open plastic cistern aquarium for choosing length × width × height (100cm × 60cm × 70cm) supports instrument as temporary.
2. in the temporary sponge for supporting the part cushioning 20cm thickness that instrument is contacted with ground, to reduce noise and vibrations by ground
Propagate.
3. add at knife long-tailed anchovy breeding water to the 2/3 of temporary foster instrument in itself, detection dissolved oxygen is 9.0mg/L, pH 7.4, water
Temperature is less than 20 DEG C, the sorbierite of addition 1.0%, by agitation fully dissolving.
4. 1 age cultivation knife long-tailed anchovy is taken, and average weight (20.5 ± 2.3) g delivers knife long-tailed anchovy, adds a cover two according to the density of 1.5g/L
Layer sunshade net.
5. count 6 hours knife long-tailed anchovy survival rates, detection survival knife long-tailed anchovy stress regulation index, including blood sugar, cortisol, second two
The transcriptional expression amount and expressing quantity of aldehydase 1, testing result are as follows：
Such as table 1, temporarily supported using the method in the present invention, 6 hours survival rates 97.0%, control group survival rate 20.0% makes
Can be significantly improved with the present invention and temporarily form motility rate.
Table 1. temporarily supports survival rate
Blood plasma cortisol and blood sugar be stress process important indicator, as depicted in figs. 1 and 2, use the method for the present invention
The violent rising of blood sugar can be significantly inhibited, is conducive to the survival of knife long-tailed anchovy.
GLO-1 is the important indicator of stress damage, and the raising of the enzyme activity can significantly reduce stress damage, pass through
Formula of the invention and operation, as shown in figure 3, the expression of GLO-1 gene significantly rises, its anti-stress ability is significantly carried
It is high.
As shown in figure 4, the protein expression level of GLO-1 also significantly rises, its resisting stress is shown from protein level
Ability is significantly improved.
Claims (6)
1. a kind of method that knife long-tailed anchovy small size pond temporarily supports resisting stress, it is characterised in that comprise the steps：
（1）Temporarily support instrument：Using open-top receptacle；
（2）Except treatment of making an uproar：By above-mentioned open-top receptacle and ground-contacting position cushioning elasticity acoustic material；
（3）Water body regulates and controls：Knife long-tailed anchovy breeding water is added in open-top receptacle, it is ensured that its dissolved oxygen is higher than 7.0mg/L, pH 7.0-
7.6, water temperature is less than 25 DEG C, adds the sorbierite of 0.6%-1.2% mass ratioes, by agitation fully dissolving；
（4）To temporarily be supported in knife long-tailed anchovy band Spinal Cord Oedema to open-top receptacle, the open-top receptacle oral area sets shade, reduces light,
Reduction stress.
2. the method that knife long-tailed anchovy small size pond according to claim 1 temporarily supports resisting stress, it is characterised in that：The open-top receptacle holds
Product is 1L-1000L.
3. the method that knife long-tailed anchovy small size pond according to claim 2 temporarily supports resisting stress, it is characterised in that：The elastic sound insulation material
Expect the sponge more than 10cm for thickness.
4. the method that knife long-tailed anchovy small size pond according to claim 2 temporarily supports resisting stress, it is characterised in that：The step（3）In
Installation of water is the 2/3 of open-top receptacle.
5. the method that knife long-tailed anchovy small size pond according to claim 1 temporarily supports resisting stress, it is characterised in that：The step（4）Hide
Male device is double-layered sunshade net.
6. the method that the knife long-tailed anchovy small size pond according to claim any one of 1-5 temporarily supports resisting stress, it is characterised in that：The step
Suddenly（4）The temporarily foster density of middle knife tapertail anchovy is 0.5-2.5 g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710168651.5A CN106804485B (en) | 2017-03-21 | 2017-03-21 | Stress-resistant method for temporarily culturing coilia ectenes in small water body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710168651.5A CN106804485B (en) | 2017-03-21 | 2017-03-21 | Stress-resistant method for temporarily culturing coilia ectenes in small water body |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106804485A true CN106804485A (en) | 2017-06-09 |
| CN106804485B CN106804485B (en) | 2019-12-17 |
Family
ID=59116172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710168651.5A Expired - Fee Related CN106804485B (en) | 2017-03-21 | 2017-03-21 | Stress-resistant method for temporarily culturing coilia ectenes in small water body |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106804485B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950636A (en) * | 2017-12-01 | 2018-04-24 | 浙江大学 | A kind of prawn live keeping agent and its method for the transport of prawn waterless keep-alive |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR0155976B1 (en) * | 1995-03-23 | 1998-11-16 | 서치영 | Anti-stress pharmaceutical composition |
| CN101647416A (en) * | 2009-09-08 | 2010-02-17 | 中国水产科学研究院淡水渔业研究中心 | Method for net fishing, transporting and temporarily culturing coilia ectenes parent fish in pool |
| CN102335138A (en) * | 2011-08-09 | 2012-02-01 | 北京福乐维生物技术有限公司 | Preparation method of nano-composite vitamin with anti-stimulation action and livestock universality |
| CN102907353A (en) * | 2011-11-21 | 2013-02-06 | 中国水产科学研究院淡水渔业研究中心 | Transportation method for coilia nasus fingerling |
| CN103026987A (en) * | 2011-10-10 | 2013-04-10 | 上海市水产研究所 | Indoor cement pond culture technique of Yangtze River Coilia ectenes |
| CN103652369A (en) * | 2013-12-04 | 2014-03-26 | 北京福乐维生物科技股份有限公司 | General-purpose livestock nano nutritional agent having anti-stress function and preparation method of the nutritional agent |
| CN104705495A (en) * | 2015-03-31 | 2015-06-17 | 江西中成人药业有限公司 | Special traditional Chinese medicine polysaccharide feed additive for pets |
| CN105532535A (en) * | 2015-12-31 | 2016-05-04 | 贵州大学 | Method for prolonging fresh time of carps in transportation |
-
2017
- 2017-03-21 CN CN201710168651.5A patent/CN106804485B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR0155976B1 (en) * | 1995-03-23 | 1998-11-16 | 서치영 | Anti-stress pharmaceutical composition |
| CN101647416A (en) * | 2009-09-08 | 2010-02-17 | 中国水产科学研究院淡水渔业研究中心 | Method for net fishing, transporting and temporarily culturing coilia ectenes parent fish in pool |
| CN102335138A (en) * | 2011-08-09 | 2012-02-01 | 北京福乐维生物技术有限公司 | Preparation method of nano-composite vitamin with anti-stimulation action and livestock universality |
| CN103026987A (en) * | 2011-10-10 | 2013-04-10 | 上海市水产研究所 | Indoor cement pond culture technique of Yangtze River Coilia ectenes |
| CN102907353A (en) * | 2011-11-21 | 2013-02-06 | 中国水产科学研究院淡水渔业研究中心 | Transportation method for coilia nasus fingerling |
| CN103652369A (en) * | 2013-12-04 | 2014-03-26 | 北京福乐维生物科技股份有限公司 | General-purpose livestock nano nutritional agent having anti-stress function and preparation method of the nutritional agent |
| CN104705495A (en) * | 2015-03-31 | 2015-06-17 | 江西中成人药业有限公司 | Special traditional Chinese medicine polysaccharide feed additive for pets |
| CN105532535A (en) * | 2015-12-31 | 2016-05-04 | 贵州大学 | Method for prolonging fresh time of carps in transportation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107950636A (en) * | 2017-12-01 | 2018-04-24 | 浙江大学 | A kind of prawn live keeping agent and its method for the transport of prawn waterless keep-alive |
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| Publication number | Publication date |
|---|---|
| CN106804485B (en) | 2019-12-17 |
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Granted publication date: 20191217 |