CN106804485B - Stress-resistant method for temporarily culturing coilia ectenes in small water body - Google Patents
Stress-resistant method for temporarily culturing coilia ectenes in small water body Download PDFInfo
- Publication number
- CN106804485B CN106804485B CN201710168651.5A CN201710168651A CN106804485B CN 106804485 B CN106804485 B CN 106804485B CN 201710168651 A CN201710168651 A CN 201710168651A CN 106804485 B CN106804485 B CN 106804485B
- Authority
- CN
- China
- Prior art keywords
- coilia ectenes
- open container
- water body
- stress
- small water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241001460967 Coilia nasus Species 0.000 title claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000012258 culturing Methods 0.000 title claims abstract description 15
- 239000012774 insulation material Substances 0.000 claims abstract description 5
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims abstract description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 4
- 239000001301 oxygen Substances 0.000 claims abstract description 4
- 239000000600 sorbitol Substances 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- 230000002180 anti-stress Effects 0.000 abstract description 11
- 230000004083 survival effect Effects 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 239000003795 chemical substances by application Substances 0.000 abstract description 4
- 238000009360 aquaculture Methods 0.000 abstract description 3
- 244000144974 aquaculture Species 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 101710092774 Glyoxalase 1 Proteins 0.000 description 7
- JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 6
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 229960000890 hydrocortisone Drugs 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 229930189936 Glyoxalase Natural products 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241001149724 Cololabis adocetus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001617 migratory effect Effects 0.000 description 1
- 230000008288 physiological mechanism Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
the invention discloses a method for temporarily culturing coilia ectenes in a small water body to resist stress, which is characterized by comprising the following steps of: (1) temporary culture tool: an open container is adopted; (2) denoising: adding elastic sound insulation material at the contact position of the open container and the ground; (3) water body regulation and control: adding water for culturing coilia ectenes into an open container, ensuring that the dissolved oxygen is higher than 7.0mg/L, the pH is 7.0-7.6, the water temperature is lower than 25 ℃, adding 0.6-1.2% of sorbitol by mass ratio, and fully dissolving by stirring; (4) transporting the coilia ectenes with water to an open container for temporary culture, wherein the mouth part of the open container is provided with a shading device, so that light is reduced, and stress is reduced. The invention has simple using tools which are conventional appliances in aquaculture and are convenient to obtain. The anti-stress agent is a single component, is non-toxic and harmless, has low cost and obvious anti-stress effect, and can improve the temporary culture survival rate of a small water body for 6 hours to over 96 percent.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and relates to an anti-stress method for temporarily breeding coilia ectenes in a small water body.
Background
Yangtze river Coilia ectenes (Coilia nasus), commonly called saury, is rare and famous reproductive migratory fish in Yangtze river, is famous for delicacy, tenderness and beauty, enjoys the first reputation of three delicacies in Yangtze river, and is an important famous and special economic species in China. However, the coilia ectenes has strong stress response and dies after water discharge, namely, temporary culture in a small water body is needed in a plurality of links in the culture process, and the stress death is easily caused in the small water body, so that serious economic loss is caused. Therefore, there is a need to develop a method for improving the survival rate of the small water temporary culture of the Yangtze river coilia ectenes, so as to reduce the mortality rate and the economic loss in the process.
In other aquatic animals, compound anti-stress agents are usually prepared from compound vitamins, Chinese herbal medicines, glucose and the like, and have a certain anti-stress effect. According to the test of the inventor, the effect of the aquatic anti-stress agent on the current market in the Yangtze river coilia ectenes is not obvious.
disclosure of Invention
The invention aims to provide an anti-stress method for temporarily culturing coilia ectenes in a small water body, which starts from a physiological mechanism of stress regulation and control of coilia ectenes and effectively improves the survival rate of temporarily culturing coilia ectenes in the small water body for 6 hours.
In order to achieve the purpose, the invention adopts the following technical scheme:
The method for temporarily culturing coilia ectenes in a small water body to resist stress is characterized by comprising the following steps:
(1) Temporary culture tool: an open container is adopted;
(2) Denoising: adding elastic sound insulation material at the contact position of the open container and the ground;
(3) Water body regulation and control: adding water for culturing coilia ectenes into an open container, ensuring that the dissolved oxygen is higher than 7.0mg/L, the pH value is 7.0-7.6, the water temperature is lower than 25 ℃, adding 0.6-1.2% of sorbitol by mass ratio, and fully dissolving by stirring;
(4) Transporting the coilia ectenes with water to an open container for temporary culture, wherein the mouth part of the open container is provided with a shading device, so that light is reduced, and stress is reduced.
Preferably: the volume of the open container is 1L-1000L.
The elastic sound insulation material is sponge with the thickness larger than 10 cm.
the sunshade device is a double-layer sunshade net.
The water amount in the step (3) is 2/3 of the open container.
The temporary culture density of the coilia ectenes in the step (4) is 0.5-2.5 g/L.
compared with the prior art, the invention has the following beneficial effects:
1. The invention has simple using tools, is a conventional appliance in aquaculture and is convenient to obtain.
2. The anti-stress agent is a single component, is non-toxic and harmless, has low cost and obvious anti-stress effect, and can improve the temporary culture survival rate of a small water body for 6 hours to over 96 percent.
Drawings
FIG. 1 is a graph showing the variation of plasma cortisol between a control group and a coilia ectenes group by the method of the present invention.
FIG. 2 is a graph showing the plasma glucose changes of coilia ectenes in a control group and a group of coilia ectenes using the method of the present invention.
FIG. 3 is a diagram showing the variation of glyoxalase 1 for coilia ectenes according to the method of the present invention.
FIG. 4 is a photograph showing comparative electrophoresis of glyoxalase 1.
Detailed Description
The present invention will be described in further detail below with reference to specific examples, but the embodiments of the present invention are not limited thereto. Reagents, equipment and methods adopted by the invention are all reagents, equipment and methods which are conventionally and commercially available in the technical field and are conventionally used in the technical field.
1. An open plastic water tank aquarium with length, width and height (100cm, 60cm and 70cm) is selected as a temporary rearing tool.
2. And a sponge with the thickness of 20cm is additionally arranged on the part of the temporary rearing tool, which is contacted with the ground, so that the noise and the vibration are reduced to be transmitted through the ground.
3. Adding culture water of coilia ectenes to 2/3 of temporary culture tool, detecting dissolved oxygen of 9.0mg/L, pH 7.4, water temperature below 20 deg.C, adding 1.0% sorbitol, and stirring to dissolve completely.
4. Taking 1-year-old cultured coilia ectenes, putting the coilia ectenes with the average weight (20.5 +/-2.3) g according to the density of 1.5g/L, and covering two layers of sunshade nets.
5. counting the survival rate of the coilia ectenes for 6 hours, and detecting stress regulation and control indexes of the surviving coilia ectenes, including the transcription expression quantity and the protein expression quantity of blood sugar, cortisol and glyoxalase 1, wherein the detection results are as follows:
As shown in Table 1, the survival rate of the temporary culture is increased by 97.0% in 6 hours and 20.0% in the control group when the temporary culture is carried out by the method of the invention.
TABLE 1 temporary rearing survival rate
plasma cortisol and blood sugar are important indexes of the stress process, and as shown in fig. 1 and fig. 2, the method of the invention can obviously inhibit the severe rise of blood sugar, thereby being beneficial to the survival of the coilia ectenes.
The glyoxalase 1 is an important index of stress injury, the improvement of the activity of the glyoxalase can obviously reduce the stress injury, and through the formula and the operation of the invention, as shown in figure 3, the expression of the glyoxalase 1 gene is obviously improved, and the anti-stress capability of the glyoxalase is obviously improved.
As shown in FIG. 4, the protein expression level of glyoxalase 1 is also significantly increased, and the protein level shows that the anti-stress capability of the glyoxalase 1 is significantly improved.
Claims (6)
1. the method for temporarily culturing coilia ectenes in a small water body to resist stress is characterized by comprising the following steps:
(1) Temporary culture tool: an open container is adopted;
(2) denoising: adding elastic sound insulation material at the contact position of the open container and the ground;
(3) Water body regulation and control: adding water for culturing coilia ectenes into an open container, ensuring that the dissolved oxygen is higher than 7.0mg/L, the pH is 7.0-7.6, the water temperature is lower than 25 ℃, adding 0.6-1.2% of sorbitol by mass ratio, and fully dissolving by stirring;
(4) Transporting the coilia ectenes with water to an open container for temporary culture, wherein the mouth part of the open container is provided with a shading device, so that light is reduced, and stress is reduced.
2. the method for temporarily culturing coilia ectenes in a small water body to resist stress according to claim 1, wherein the method comprises the following steps: the volume of the open container is 1L-1000L.
3. The method for temporarily culturing coilia ectenes in a small water body to resist stress according to claim 2, wherein the method comprises the following steps: the elastic sound insulation material is sponge with the thickness larger than 10 cm.
4. the method for temporarily culturing coilia ectenes in a small water body to resist stress according to claim 2, wherein the method comprises the following steps: the water amount in the step (3) is 2/3 of the open container.
5. The method for temporarily culturing coilia ectenes in a small water body to resist stress according to claim 1, wherein the method comprises the following steps: and (4) the shading device is a double-layer shading net.
6. the method for temporarily culturing coilia ectenes in a small water body to resist stress according to any one of claims 1 to 5, wherein the method comprises the following steps: the temporary culture density of the coilia ectenes in the step (4) is 0.5-2.5 g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710168651.5A CN106804485B (en) | 2017-03-21 | 2017-03-21 | Stress-resistant method for temporarily culturing coilia ectenes in small water body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710168651.5A CN106804485B (en) | 2017-03-21 | 2017-03-21 | Stress-resistant method for temporarily culturing coilia ectenes in small water body |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106804485A CN106804485A (en) | 2017-06-09 |
| CN106804485B true CN106804485B (en) | 2019-12-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710168651.5A Expired - Fee Related CN106804485B (en) | 2017-03-21 | 2017-03-21 | Stress-resistant method for temporarily culturing coilia ectenes in small water body |
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| Country | Link |
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| CN (1) | CN106804485B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107950636B (en) * | 2017-12-01 | 2021-05-11 | 浙江大学 | Prawn survival keeping agent and method for prawn anhydrous survival keeping transportation by using same |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101647416A (en) * | 2009-09-08 | 2010-02-17 | 中国水产科学研究院淡水渔业研究中心 | Method for net fishing, transporting and temporarily culturing coilia ectenes parent fish in pool |
| CN102335138A (en) * | 2011-08-09 | 2012-02-01 | 北京福乐维生物技术有限公司 | Preparation method of nano-composite vitamin with anti-stimulation action and livestock universality |
| CN102907353A (en) * | 2011-11-21 | 2013-02-06 | 中国水产科学研究院淡水渔业研究中心 | Transportation method for coilia nasus fingerling |
| CN103026987A (en) * | 2011-10-10 | 2013-04-10 | 上海市水产研究所 | Indoor cement pond culture technique of Yangtze River Coilia ectenes |
| CN103652369A (en) * | 2013-12-04 | 2014-03-26 | 北京福乐维生物科技股份有限公司 | General-purpose livestock nano nutritional agent having anti-stress function and preparation method of the nutritional agent |
| CN105532535A (en) * | 2015-12-31 | 2016-05-04 | 贵州大学 | Method for prolonging fresh time of carps in transportation |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR0155976B1 (en) * | 1995-03-23 | 1998-11-16 | 서치영 | Anti-stress pharmaceutical composition |
| CN104705495A (en) * | 2015-03-31 | 2015-06-17 | 江西中成人药业有限公司 | Special traditional Chinese medicine polysaccharide feed additive for pets |
-
2017
- 2017-03-21 CN CN201710168651.5A patent/CN106804485B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101647416A (en) * | 2009-09-08 | 2010-02-17 | 中国水产科学研究院淡水渔业研究中心 | Method for net fishing, transporting and temporarily culturing coilia ectenes parent fish in pool |
| CN102335138A (en) * | 2011-08-09 | 2012-02-01 | 北京福乐维生物技术有限公司 | Preparation method of nano-composite vitamin with anti-stimulation action and livestock universality |
| CN103026987A (en) * | 2011-10-10 | 2013-04-10 | 上海市水产研究所 | Indoor cement pond culture technique of Yangtze River Coilia ectenes |
| CN102907353A (en) * | 2011-11-21 | 2013-02-06 | 中国水产科学研究院淡水渔业研究中心 | Transportation method for coilia nasus fingerling |
| CN103652369A (en) * | 2013-12-04 | 2014-03-26 | 北京福乐维生物科技股份有限公司 | General-purpose livestock nano nutritional agent having anti-stress function and preparation method of the nutritional agent |
| CN105532535A (en) * | 2015-12-31 | 2016-05-04 | 贵州大学 | Method for prolonging fresh time of carps in transportation |
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| CN106804485A (en) | 2017-06-09 |
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