RU2545891C2 - Method of treating dogs with babesiasis and producing preparation of male bee larvae and beet juice for treating them - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: group of inventions refers to veterinary science. Substance of the method of treating dogs in babesiasis and producing a preparation of male bee larvae and beet juice for treating them consists as follows. The preparation Bebazan 4% is introduced into the dogs suffering babesiasis in a dose of 0.1 ml per one kg of dog's body weight, intramuscularly two times every 24 hours; that is added with introducing an alcohol preparation of male bee larvae and beet juice orally at 0.5ml per one kg of body weight once a day for 9-11 days in dogs. The preparation is made from male bee larvae and beet juice. That is ensured by gathering 9-10-day male bee larvae together with larval food with adding 96° ethanol at 1:10, one portion of alcohol and nine portions of larvae, freezing at -18°C for 6 months, unfreezing to room temperature for 6 hours and homogenising, e.g. in a blender. Red beet juice is extracted, e.g. by means of a juice extractor. Further, one portion of homogenised male bee larvae is added with one portion of beet juice, eight portions of 40° ethanol with the mixture to be kept in a dark place at room temperature for 14-15 days with agitating occasionally once a day. The infusion is then filtered through 6 gauze layers and bottled in flasks 100 ml and closed with rubber plugs.

EFFECT: improving the therapeutic effect, reducing the length and cost of treatment and increasing the body resistance.

2 cl, 8 ex, 5 dwg

Description

FIELD OF THE INVENTION

The invention relates to veterinary medicine, in particular to a method for treating dogs with babesiosis and obtaining a preparation from bee drones and beet juice for their treatment, and can be used in a method of treating dogs with babesiosis.

State of the art

A drug is known for the treatment of dog pyroplasmosis, including Berenil as an active substance and water as a solvent, and dimephosphon as an additional solvent, in the following ratio of components, g per 100 ml of water: berenyl 17.0-18.0; dimephosphon - 0.5-1.0, water for injection - up to 100 ml. The drug is rapidly absorbed and has a prolonged effect (see US Pat. RF No. 2347558, class A61K 31/00, A61P 33/00, publ. February 27, 2009).

The disadvantage of this drug is its short storage time and its high toxicity to dogs.

A known drug for the treatment of animal pyroplasmidoses and a method for its use, comprising the etiotropic substance ethidium bromide (Ethidium Bromide). For the treatment of animal pyroplasmidoses, ethidium bromide and azidine are intramuscularly administered as a debridement pair, with ethidium bromide being initially administered, and then after 24 hours, azidine in a dosage of 3.5 mg per 1 kg of body weight. The use of the drug and method of treatment can reduce treatment time and reduce toxicity (see US Pat. RF No. 2119792, class A61K 31/055, publ. 10.10.1998).

The disadvantage of this method is the insufficiently fast recovery of hematological and biochemical parameters of blood serum and the health of dogs.

A known method for the treatment of dogs babesiosis, including the introduction of drugs based on diminazene acetate and drugs of symptomatic therapy. In this case, preparations based on diminazene acetate are administered intramuscularly twice with an interval of 24 hours at a dose of 2.5-3.0 mg / kg body weight for the active substance in the form of a 7% solution. At the same time, drugs of symptomatic therapy are administered. As drugs for symptomatic therapy, enter: riboxin and sulfocamphocaine 1-2 times a day for 5 days in the form of subcutaneous injections at a dose of 0.5-1.0 ml per animal, depending on live weight, prednisone at a dose of 0.5-1 , 0 ml per animal, depending on live weight intramuscularly once a day for 3 days, ethamylate in the form of a 12.5% solution intramuscularly at a dose of 1.0 ml per 20 kg of live weight once a day for the first 2-3 day of treatment and Essential Forte at a dose of 2-5 ml per animal, depending on live weight intravenously for 5 days. The invention, combining measures of etiotropic and pathogenetic effects, allows to obtain a good therapeutic effect, to avoid complications and significantly reduce mortality (see US Pat. RF No. 2407542, class A61K 39/01, publ. 12/27/2010).

The disadvantage of this method is the high cost and complexity of the treatment.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a method of treating dogs, which includes administering to dogs antiprotozoal drug Babesan 4 and 12% in an injectable form subcutaneously or intramuscularly at doses of 0.1 ml and 0.03 ml per kg body weight, respectively, once. Repeated injections for 16-24 hours were performed only in severe cases of the disease. In many cases of the disease, different courses of pathogenetic therapy were used, adapted for mild, moderate, and severe forms of dog pyroplasmosis (saline, ascorbic acid, thiamine bromide, cyancobalamin, gamavit, essentiale forte, karsil, analgin, no-spa, dexamethasone or diphenhydramine, cocarboxylase , sulfocamphocaine, cordiamine, furosemide, bicillin-3) (S. V. Engashev, M. D. Novak, E. Kh. Daugalieva, O. Yu. Mazitova, M. S. Pitetskiy. “Babezan’s efficacy in dogs pyroplasmosis // 21st Century Veterinary Medicine: Innovation, Exchange of Experience and Prospects for Development: Proceedings of the International scientific and practical conference - VPO "Saratov SAU" 2012 str.79-83)..

The disadvantage of this method is the likelihood of recurrence of the disease and the slow recovery of dogs after the disease.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a method of preparing a feed additive from drones larvae to increase the resistance of dogs in parasitoses, which includes the collection of drones larvae, purga with subsequent freezing, homogenization with the addition of 40% homogenate alcohol at the rate of 20 g per 100 ml of alcohol, infusion in a dark place for 13-14 days, followed by straining in sterile bottles. 9-10-day-old larvae are collected along with waxed honeycombs, honey and perga. Freezing is carried out at a temperature of - (16 ° C) - (- 18 ° C), and then without preliminary thawing, the collection is homogenized in a blender for 30-40 seconds until a homogeneous mass is obtained and infusion is carried out at a temperature of (+ 18 ° C) - ( + 23 ° C) (see Pat. RF No. 2402920, class A23K 1/00, published on November 10, 2010).

The disadvantage of this method is the insufficiently high therapeutic efficacy in case of anemia that develops as a result of the disease of dogs with babesiosis.

Disclosure of invention

The objective of the invention is to develop a method of treating dogs suffering from babesiosis with a high therapeutic effect, reducing treatment time and increasing the body's resistance, as well as reducing treatment costs.

The technical result that can be achieved using the present invention is to increase the therapeutic effect, reduce treatment time and increase body resistance, as well as reduce treatment costs.

The technical result is achieved using a method of treating dogs with babesiosis, including the introduction of the drug Babesan 4% at a dose of 0.1 ml per kg of body weight of the dog, intramuscularly, twice, with an interval of 24 hours, while the dogs are additionally given oral alcohol from bee larvae and red beet juice obtained by the method according to claim 2, at the rate of 0.5 ml per kg of live weight once a day for 9-11 days.

The technical result is achieved using a method of producing a preparation from bee drones and beetroot juice for treating dogs with babesiosis, including collecting drones of 9-10 days old together, to which 96 ° ethanol is then added at the rate of one part of alcohol and nine parts of larvae, then the mixture is frozen at -18 ° C, incubated for 24-48 hours, thawed to room temperature for 6 hours and homogenized, then one part of red beet juice is added to one part of the bee drone larvae homogenate, eight parts are added th 40 th ° ethyl alcohol and soaked in a dark place at room temperature for 14-15 days, periodically, once per day by shaking, then the infusion is filtered, filled into vials and sealed.

Thus, an alcohol preparation containing one part of homogenized drone larvae, one part of red beet juice and eight parts of 40 ° ethanol allows to obtain a high therapeutic effect, reduce treatment time and increase the body's resistance, reduce treatment costs.

The result of increasing the resistance of the dog’s organism is achieved due to the content of the protein complex, free amino acids, carbohydrates, minerals, and biologically active substances in the alcohol preparation from bee darlings and red beet juice: a large number of functional groups of enzymes, as well as testosteroid hormones (see "The use of drone brood for cosmetic purposes", A.G. Bachinsky, A.N. Decina, J. "Beekeeping", No. 5, 1998, p. 54) of progesterone extradiol. Dried drone larvae contain 28 amino acids, including 9 essential ones. Among the latter, there is a significant amount of lysine, isoleucine, phenylalanine (see "Biological and nutritional value of Bilar powders", A.I. Cherkasov, I.A. Prokhoda, J. "Beekeeping", No. 2, 2005, p. 50- 51). The positive effect of beetroot juice is associated with the presence of B vitamins, vitamins C and PP, and a high content of calcium, magnesium, iron and zinc salts involved in the hematopoiesis.

The essence of the method of treating dogs with babesiosis and obtaining the drug from the larvae of drones of bees and beet juice for their treatment is as follows.

In case of babesiosis, dogs are treated with Babesan 4% at a dose of 0.1 ml per kg of dog’s body weight, intramuscularly, twice, with an interval of 24 hours, while the dogs are additionally orally administered an alcohol preparation from bee drone larvae and red beet juice from calculation of 0.5 ml per kg of live weight once a day for 9-11 days.

The drug is made from bee drone larvae and red beet juice. For this: in the apiary, drones of 9-10 days old are collected together, to which 96 ° ethanol is added at the rate of one part of alcohol and nine parts of larvae, then they are frozen at -18 ° C, the shelf life is up to 6 months, then thawed to room temperature for 6 hours and homogenized, for example, in a blender. Juice is separated from red beets using, for example, a juicer. Subsequently, to one part of the homogenate of bee drones, one part of beet juice is added, eight parts of 40 ° ethanol are added and insisted in a dark place at room temperature for 14-15 days, periodically, once a day, shaking. Then the infusion is filtered through 6 layers of gauze and poured into 100 ml bottles and sealed with rubber stoppers. The drug has the form of an opalescent burgundy color.

Brief description of drawings and other materials

In FIG. 1 shows a method for treating dogs with babesiosis and obtaining a preparation from bee drones larvae and beet juice for their treatment, the chemical composition of bee drone larvae, table 1.

In FIG. 2 - the same chemical composition of red beet juice, table 2.

In FIG. 3 - the same, the number of red blood cells million / μl in dogs treated with the proposed method, in comparison with the traditional, table 3.

In FIG. 4 - the same, the number of leukocytes in dogs, thousand / μl, μl in dogs treated with the proposed method in comparison with the traditional, table 4.

In FIG. 5 - the same, the amount of hemoglobin g / l in dogs, μl in dogs treated with the proposed method in comparison with the traditional, table 5.

The implementation of the invention

Examples of specific performance of the method of obtaining the drug from the larvae of drones of bees and beet juice

Example No. 1. In the apiary, drones of bees of 9-10 days old are collected, to which 96 ° ethanol is then added at the rate of 1 part of alcohol and 9 parts of larvae, then the mixture is frozen at -18 ° C (shelf life - up to 6 months), then thawed to room temperature for 6 hours and homogenized, for example in a blender. Red beet juice is obtained using, for example, a juicer, and then mixed in a ratio of 2-3 parts of the homogenate of bee drones, 3-4 parts of beet juice, the rest (4-5 parts) of 40 ° ethanol and insist in a dark place at room temperature for 14-15 days, periodically (1 time per day) shaking.

Result. By the 10th day, the infusion begins to cloud, by the 14th day flakes appear in it, which indicates the active reproduction of microorganisms due to the increased concentration of beet sugars and a low concentration of ethyl alcohol.

Example No. 2. The collection and preparation of larvae and beetroot juice is carried out analogously to example 1, and the components are mixed in the ratio of 2-3 parts of bee drones larvae, 1-2 parts of beet juice, the rest (6-7 parts) of 40 ° ethanol, and insist in a dark place at room temperature for 14-15 days, periodically (1 time per day) shaking. Then the infusion is filtered through 6 layers of gauze. A burgundy opalescent liquid is obtained.

Result. In the process of filtering, a lot of material from drones larvae remains on the filter material. In addition, when viewing the drug into the light, undissolved suspensions, particles of components are found.

Example No. 3. The collection and preparation of larvae and beetroot juice is carried out analogously to example 1, and the components are mixed in a ratio of 1-2 parts of bee drones larvae, 1-2 parts of beet juice, the rest (7-8 parts) of 40 ° ethanol, and insist in dark place at room temperature for 14-15 days, periodically (1 time per day) shaking, then the infusion is filtered through 6 layers of gauze. A burgundy opalescent liquid is obtained.

Result. When viewed into the light, the drug does not contain particles of components and undissolved suspensions.

Example No. 4. The collection and preparation of larvae and beetroot juice is carried out analogously to example 1, and the components are mixed in a ratio of 1-2 parts of bee drones larvae, 1-2 parts of beet juice, the rest (7-8 parts) of 40 ° ethanol, but insist in dark place at room temperature for 20-21 days, periodically (1 time per day) shaking, then the infusion is filtered through 6 layers of gauze. A burgundy opalescent liquid is obtained.

Result. When viewed into the light, the drug does not contain particles of components and undissolved suspensions.

Since, according to examples 3 and 4, a similar result is obtained, but according to example 3, less time is required for its preparation, in the future, when testing the effectiveness, use the drug obtained in example 3.

Examples of specific performance of the method of treating dogs with babesiosis.

Example No. 1. Azidine (7% solution) is administered to ten dogs at a dose of 3.5 mg / kg, intramuscularly, twice, with an interval of 24 hours, with the preliminary administration of analgin and diphenhydramine; homeopathic medicine Liarsin at a dose of 1 ml / 10 kg and Hemobalance at a dose of 1 ml / 25 kg, three times, with an interval of 24 hours.

Result: In dogs, a day after the administration of azidine, a decrease in temperature to 39.3-39.7 ° C is observed, however, the animals remain lethargic, selective appetite, the level of hemoglobin in the urine is 50-100 mg Hb / 1. In smears of peripheral blood, single babesias were detected (parasitemia 0.4%). 24 hours after a second injection of azidine, babesia was not found in peripheral blood smears, appetite was restored, hemoglobin traces in the urine, after physical exertion the animals noticeably tire, become lethargic, rapid breathing and palpitations are noted. When examining dogs on the eleventh day from the start of treatment, the general condition of the animals is satisfactory, however, the owners notice some lethargy after physical exertion, body weight is lower than 3.5% ± 0.5% compared with the initial intake. The cost of veterinary drugs ranged from 544 to 1256 rubles. depending on the live weight of the dog.

Example No. 2. Ten dogs are administered Babesan at a dose of 0.1 ml / kg, once, subcutaneously, with prior administration of analgin and diphenhydramine; homeopathic medicine Liarsin at a dose of 1 ml / 10 kg and Hemobalance at a dose of 1 ml / 25 kg, three times, with an interval of 24 hours.

Result: In dogs, a day after the administration of Babesan, a decrease in temperature to 39.0-39.4 ° C, restoration of appetite are observed, in the urine there are traces of hemoglobin. In smears of peripheral blood in erythrocytes - single babesias (parasitemia - up to 0.3%). In 3 dogs, two days after the improvement, the body temperature again increased to 40.1-40.2 ° C, which forced a repeated administration of the drug. In the future, in order to avoid relapse, this treatment regimen was abandoned.

Example No. 3. Babesan is administered to ten dogs at a dose of 0.1 ml / kg, twice, subcutaneously, with an interval of 24 hours, with the preliminary administration of analgin and diphenhydramine. Along with specific treatment, an alcohol preparation is prescribed from drones and beet larvae, at a dose of 0.5 ml / 1 kg, orally, for 10 days, as well as an easily digestible diet.

Example No. 4. Babesan is administered to ten dogs at a dose of 0.1 ml / kg, twice, subcutaneously, with an interval of 24 hours, with the preliminary administration of analgin and diphenhydramine. Along with specific treatment, an alcohol preparation is prescribed from drones and beets larvae at a dose of 0.5 ml / 1 kg, orally, for 10 days, as well as a readily digestible diet.

Result: In dogs, a day after the administration of Babesan, a decrease in temperature to 39.0-39.4 ° C, restoration of appetite are observed, in the urine there are traces of hemoglobin. In smears of peripheral blood in erythrocytes - single babesias (parasitemia - up to 0.3%). A day after the repeated administration of Babesan, babesia is not found in blood smears, animals are active, the test for the presence of hemoglobin in the urine is negative. In a clinical examination of animals on the eleventh day from the start of treatment, after the completion of the course of the alcohol preparation from drones and beet larvae, complete recovery was noted, as well as an increase in body weight by 6.2 ± 1.2% compared with the weight at the initial intake. The cost of veterinary drugs ranged from 213 to 284 rubles. depending on the live weight of the dog.

Example No. 5. Babesan is administered to ten dogs at a dose of 0.1 ml / kg, twice, subcutaneously, with an interval of 24 hours, with the preliminary administration of analgin and diphenhydramine. Along with specific treatment, an alcohol preparation of drones and beets larvae is prescribed at a dose of 1 ml / 1 kg, orally, for 14 days, as well as a readily digestible diet.

In dogs, one day after the introduction of Babesan, similar changes in the clinical status are observed, as in experiment No. 4. During a clinical examination of animals on the fifteenth day from the start of treatment, after the course of the alcohol preparation from drones and beet larvae was completed, a complete recovery was noted, as well as an increase in body weight by 6.4 ± 0.8% compared with the weight at the initial intake.

Thus, the most optimal way to treat dogs with babesiosis, described in examples 4 and 5, because the combination of drugs described in these examples and the scheme of their use contribute to a high therapeutic effect, reduce treatment time and increase the body's resistance and reduce treatment costs. The results of studies of the effect of the method of treating dogs with babesiosis on the morphological composition of the blood are shown in tables 3, 4, 5.

The result of increasing the resistance of a dog’s organism is achieved due to the content of a protein complex, free amino acids, carbohydrates, minerals, as well as biologically active substances in the alcohol preparation from beet larvae of drones. So, the composition of 9-10-day-old drones larvae includes a large number of functional groups of enzymes, as well as testosteroid hormones (see "Using drone brood for cosmetic purposes", A. G. Bachinsky, A. N. Decina, J. " Beekeeping ”, No. 5, 1998, p. 54) of progesterone extradiol, and the proteins are mainly free amino acids and easily digestible oligopeptides. 28 amino acids were found in dried drone larvae, including 9 irreplaceable ones. Among the latter, there is a significant amount of lysine, isoleucine, phenylalanine (see "Biological and nutritional value of Bilar powders", A.I. Cherkasov, I.A. Prokhoda, J. "Beekeeping", No. 2, 2005, p. 50- 51).

Table 1 shows the chemical composition of the homogenate from 9-10-day drones. Beetroot juice is widely used in folk medicine as a remedy for the treatment and prevention of anemia as a detoxifier after infectious diseases as a restorative agent. The positive effect of beetroot juice is associated with the presence of B vitamins, vitamins C and PP, and a high content of calcium, magnesium, iron and zinc salts involved in the hematopoiesis (Table 2). The invention in comparison with the prototype and other known solutions has the following advantages:

- has a high therapeutic effect;

- helps to reduce treatment time and increase the body's resistance;

- allows you to reduce the cost of treatment.

Claims (2)

1. A method of treating dogs with babesiosis, including the introduction of Babesan 4% at a dose of 0.1 ml per kg of dog’s body weight, intramuscularly, twice, with an interval of 24 hours, characterized in that the dogs are additionally orally administered with an alcohol preparation from larvae of drones of bees and juice of red beet obtained by the method according to claim 2, at the rate of 0.5 ml per kg of live weight once a day for 9-11 days. 2. A method of producing a preparation from bee drones and beetroot juice for treating dogs with babesiosis, comprising collecting drones larvae of 9-10 days of age, to which 96 ° ethanol is then added at the rate of one part alcohol and nine parts larvae, then the mixture is frozen at -18 ° C, incubated for 24-48 hours, thawed to room temperature for 6 hours and homogenized, then one part of red beet juice is added to one part of the bee drone larvae homogenate, eight parts of 40 ° ethanol are added and insist in the dark month e at room temperature for 14-15 days, periodically, once per day by shaking, then the infusion is filtered, filled into vials and sealed.

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