RU2280463C1 - Method for producing preparation increasing organism resistance - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: method involves washing dead bee bodies in 0.5% household soda solution and then three times in cooled boiled water, drying and individual grinding to bring dead bees bodies and ambrosia to dust state and mixing them in equal volumes, treating with ultrasound under known conditions and adding 40% ethyl alcohol, making infusion in dark place during given time, filtering, diluting with twice-boiled water to reach 20% alcohol concentration and prepackaging.

EFFECT: enhanced effectiveness of treatment; increased hemoglobin, erythrocyte number and improved biochemical blood properties.

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Description

FIELD OF THE INVENTION

The invention relates to veterinary medicine, in particular to methods for producing preparations from bee bread and death, and can be used to increase the general and specific resistance of the animal organism.

State of the art

A known method of preparing a drug for stimulating the body of animals from drones larvae, including collecting larvae, their homogenization with the addition of a stabilizing medium, while before homogenizing drones larvae are frozen at a temperature of (-4; -5 ° C) for 12-14 hours, then thawed to a temperature of + 18 ° C and homogenize for 3-5 minutes, while a stabilizing medium using a 5% glucose solution in a ratio of 1:10 with the addition of a 5% solution of chloroform or 2% salicylic acid, followed by sublimation, the limitation is carried out in a vacuum chamber at a temperature of heating the shelves to 30 ° -45 ° C, while reaching a temperature of the drug 20-22 ° C, the temperature of the shelves is reduced to 25 ° C and drying is carried out for 22-24 hours (see. "Harvesting drones ", V.I. Lebedev, M.A. Legovich, Research Institute of Beekeeping, J. Beekeeping, No. 3, 2003, p.52-54).

The disadvantage of this method is the short storage time of the drug.

As well as a method for producing an alcoholic extract of large wax moth larvae, which includes collecting larvae, their homogenization with the addition of a stabilizing medium (see "New Life of the Old Medication", A.K. Rachkov, M.N. Kondrashova, N.A. Spiridonov, f Beekeeping, No. 5, 2000, pp. 58-59).

The disadvantage of this method is the short storage time of the drug.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a method of obtaining a therapeutic and prophylactic product "SHIFA" from bee bread, including the placement of raw materials in an airtight chamber, reducing the pressure in it to 100 mm Hg followed by processing the raw materials in the chamber using a microwave field, while the microwave heating is carried out so that the temperature in the chamber does not exceed 45 ° C until the desired product with a moisture content of not more than 5% is obtained, the resulting product has a high content of vitamins, microelements, essential amino acids, as well as the ability to preserve their healing properties during long-term storage (see US Pat. RF No. 2086245, class A 61 K 35/64, publ. 08/10/1997).

The disadvantage of this method is the high cost of obtaining a therapeutic product.

Disclosure of invention

The technical result that can be achieved using the proposed method is to increase the overall resistance, increase the number of red blood cells, hemoglobin and biochemical blood parameters of animals, obtain a cheaper and more affordable drug, simplify the production method.

The technical result is achieved using a method of obtaining a drug to increase the body's resistance, including processing of bee bread, while the bees are killed in a 0.5% solution of baking soda, then - three times in boiled water, dried and separately ground to a dusty state of bees and bee bread, they are mixed in equal volumes, sonicated at a power of 400 W, a current strength of 0.7 A, a frequency of 35 kHz for 10-15 minutes, and 40% ethyl alcohol is added to the resulting mixture at the rate of 20 g of the mixture per 100 ml of alcohol, insist in t mnom place for 14 days, filtered, diluted with water, boiled twice with 20% alcohol and packaged.

The essence of the method of obtaining the drug to increase the resistance of the body

The drug is made from dead bees and bee bread. To do this, the bee killing at the beginning was washed in a 0.5% solution of baking soda, then washed three times with boiled water, dried.

Then crush and bee bread are crushed to a dust-like state separately and mixed in equal volumes, sonicated at a power of 400 W, a current strength of 0.7 A, a frequency of 35 kHz for 10-15 minutes.

To the resulting mixture add 40% ethyl alcohol at the rate of 20 g of the mixture per 100 ml of alcohol and insist in a dark place for 14 days.

Then it is filtered through paper filters and diluted with twice boiled water to 20% alcohol.

Pour the drug into 500 ml vials, closing with sterile plugs.

The implementation of the invention

Examples of specific performance of the method of obtaining the drug to increase the resistance of the body.

Experience No. 1. Dead bees - grind up the grind in a meat grinder and pour 40% alcohol at a rate of 1: 5 and insist in a dark place for 10 days. Filter through cotton and pour into bottles, and use.

The resulting preparation was not sterile and did not give a high effect in the symptomatic treatment of pyroplasmidoses in animals. In addition, a lot of material from the dead was left on the filter.

Experience No. 2. Carried out similarly to experiment No. 1, but to the ground podomore add ground pork and extracted with 40% alcohol solution (1: 5) for 14 days. Filter through gauze swabs and use.

The resulting preparation was also not sterile, and inflammation occurred in animals at the site of intramuscular injection.

Experience No. 3. The death of bees at the beginning is washed in a 0.5% solution of baking soda, then washed three times with boiled water, dried on a cotton cloth, decomposing it with a thin layer.

Then, in a coffee grinder or in a special mill, crushing and scorching separately are crushed to a dust-like state and mixed in equal volumes, sonicated with parameters: power 400 W, current strength 0.7 A, frequency 35 kHz for 10-15 minutes.

To the resulting mixture add 40% alcohol at the rate of 20 g of the mixture per 100 ml of alcohol and insist in a dark place for 14 days.

Then it is filtered through paper filters and diluted with twice boiled water to 20% alcohol.

The drug is poured into 500 ml vials, closed with sterile plugs and used.

Experience No. 4. Carried out similarly to experiment No. 3, but insist the mixture in a dark place for 20 days.

The resulting preparation did not cause inflammation, because It was sterile and had a positive effect in the symptomatic treatment of pyroplasmidoses, but its preparation takes longer.

The drug obtained by the claimed method was tested on rabbits at a dose of 0.25 ml per 1 kg of live weight. The drug was administered to the experimental group of animals intravenously 1 time per day for 5 days, the drug was not administered to the control group. Blood was examined before drug administration and 7 days after the start of the experiment. In the blood, the amount of hemoglobin, erythrocytes, leukocytes, total protein, albumin, globulins, t- and b-lymphocytes, lysozyme, bactericidal and phagocytic activity of blood serum was determined. The research results are presented in tables No. 1 and No. 2.

Table number 1
Morphological blood parameters of rabbits (M ± m) Group of animals Hemoglobin, g / l Red blood cells, 10 ¹² / l White blood cells, 10 ⁹ L Total protein, g / l before drug administration experienced 117 ± 3.1 5.3 ± 0.09 7.5 ± 0.2 60.3 ± 0.17 control 105 ± 4.2 4.9 ± 0.11 8.2 ± 0.15 60.7 ± 0.15 in 7 days experienced 123 ± 4.0 6.1 ± 0.13 7.3 ± 0.19 63.0 ± 0.21 control 110 ± 5.1 4.7 ± 0.12 8.1 ± 0.17 61.1 ± 0.16

Table number 2
Indicators of the natural resistance of the body of rabbits (M ± m) group of animals Albumin,% Globulins,% t-lymph. % in lymph. % α β γ before drug administration Experienced 54.7 ± 0.07 8.06 ± 0.50 6.35 ± 0.31 14.2 ± 0.40 64.0 ± 0.42 18.0 ± 0.45 Control 55.0 ± 0.21 7.19 ± 0.16 6.22 ± 0.17 13.0 ± 0.12 64.5 ± 0.09 17.8 ± 0.17 in 7 days Experienced 65.3 ± 0.11 10.0 ± 0.43 9.6 ± 0.09 16.8 ± 0.21 66.0 ± 0.21 20.0 ± 0.44 Control 54.8 ± 0.35 7.21 ± 0.20 6.1 ± 0.44 13.5 ± 1.02 64.0 ± 0.11 18.0 ± 0.20 (Continuation of table No. 2)
Indicators of the natural resistance of the body of rabbits (M ± m) group of animals LASK,% BASQUE,% FASK,% before drug administration Experienced 14.35 ± 0.19 20.0 ± 0.17 30.3 ± 0.32 Control 15.59 ± 0.21 21.4 ± 0.35 29.61 ± 0.27 in 7 days Experienced 22.60 ± 0.11 37.5 ± 0.19 35.0 ± 0.41 Control 16.0 ± 0.30 21.7 ± 0.22 31.1 ± 0.25

Analyzing the data obtained, it can be seen that compared with the control group in the experimental group, the number of red blood cells increased by 15.1%, hemoglobin by 5.1%, total protein by 4.5%, albumin by 19.4%, globulins: α - 24 , 0%, β - 51.1%, γ - 18.3%; the number of t-lymphocytes increased by 3.1%, b-lymphocytes by 11.1%; the bactericidal activity of blood serum increased by 87.5%, lysozyme - 57.4% and phagocytic - 15.5%, the number of leukocytes in the blood of animals of the compared groups during the study was almost at the same level.

Our studies have shown that the drug obtained by the claimed method has a positive effect on the indicators of the natural resistance of the animal organism.

The invention in comparison with the prototype and other known solutions has the following advantages:

- the drug increases the overall resistance of the body;

- has a high positive effect in case of anemia caused by blood parasitic diseases of animals;

- the drug can be administered subcutaneously, intramuscularly, intravenously, orally because it is sterile;

- easy to manufacture;

- has a shelf life of 2 years.

Claims (1)

A method of obtaining a drug to increase the body’s resistance, including processing of bee bread, characterized in that the bees are dead in a 0.5% baking soda solution, then three times in boiled water, dried and separately bees and bee are killed and pulverized, mixed in equal volumes, is treated with ultrasound at a power of 400 W, a current strength of 0.7 A, a frequency of 35 kHz for 10-15 minutes, and 40% ethyl alcohol is added to the resulting mixture at the rate of 20 g of the mixture per 100 ml of alcohol, insisted dark place for 14 days, fil rub, diluted twice with boiled water to 20% alcohol and packaged.