RU2402920C1 - Method for preparation of fodder additive from male bee larvae to increase resistance of dogs organism in case of parasitic disease - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to veterinary science, in particular to methods for preparation of fodder additives from male bee larvae and may be used to increase overall and specific resistance of dogs organism. Method includes gathering male bee larvae, bee-bread, with further freezing, homogenisation with addition of 40% alcohol into homogenate in amount of 20 g per 100 ml of alcohol, infusion in dark area for 13-14 days with further colation and pouring into sterile bottles. Larvae of 9-10 days are gathered with honeycomb wax, honey and bee-bread. Freezing is carried out at the temperature of (-16°C)-(-18°C), and then without preliminary unfreezing, the mix is homogenised in blender for 30-40 seconds to produce homogeneous mass, and infusion is produced at the temperature of (+18°C)-(+23°C).

EFFECT: invention makes it possible to increase overall resistance of dogs organism, to increase quantity of erythrocytes and hemoglobin and biochemical parametres of dogs blood, assists in quick increase of body weight, to produce cheaper and affordable preparation, to reduce losses of nutrient and biologically active substances contained in honeycomb cells.

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Description

FIELD OF THE INVENTION

The invention relates to veterinary medicine, in particular to methods for preparing feed additives from drones, and can be used to increase the general and specific resistance of dogs.

State of the art

A known method of preparing an alcoholic extract of the larvae of the large wax moth, including the collection of larvae, their homogenization with the addition of a stabilizing medium (see "New life of the old medicine", A. K. Rachkov, M. N. Kondrashova, N. A. Spiridonov, f. Beekeeping, No. 5, 2000, p. 58-59).

The disadvantage of this method is the short storage time of the drug.

A known method of preparing a drug for stimulating the body of animals from drones larvae, including collecting larvae, their homogenization with the addition of a stabilizing medium, while before homogenizing drones larvae are frozen at a temperature of (-4; -5 ° C) for 12-14 hours, then thawed to a temperature of + 18 ° C and homogenize for 3-5 minutes, while a stabilizing medium using a 5% glucose solution in a ratio of 1:10 with the addition of a 5% solution of chloroform or 2% salicylic acid, followed by sublimation, imitation is carried out in a vacuum chamber at a temperature of shelf heating to 30 ° -45 ° C, while reaching a temperature of the drug 20-22 ° C, the temperature of the shelves is reduced to 25 ° C and drying is carried out for 22-24 hours (see. “Preparation of drones larvae ", V.I. Lebedev, M. A. Legovich, Research Institute of Beekeeping, J. Beekeeping, No. 3, 2003, p. 52-54).

The disadvantage of this method is the short storage time of the drug.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a method of preparing the drug to increase the general and specific resistance of the animal organism, including collecting bee bread, while additionally collecting drones larvae are carried out, after which drones larvae are frozen to (-4 ° С ) - (- 5 ° С), then thawed at a temperature of + 18 ° С, crushed until a homogeneous mass is formed, the homogenate of the juvenile phases of the drones is combined with the ground pollen to a dust-like state in wearing 1: 1 followed by sonication of the mixture with parameters: power 400 W, current 0.7 A, frequency 35 kHz for 10-15 minutes and pour 40% alcohol at the rate of 20 g of the mixture per 100 ml 40% - alcohol, the composition is infused in a dark place for 14 days, then filtered, sterile water is added to the filtrate to a concentration of 20% in tincture of alcohol.

(See Pat. RF 2291704, class IPC ⁷ A61K 35/64, publ. 20.01.2007).

The disadvantage of this method is the significant loss of nutrients and biological substances and wax contained in the cells of the cells, when removing the larvae of drones.

Disclosure of invention

A method of preparing a feed additive from drones larvae to increase the resistance of dogs in case of parasitosis, including collecting drones larvae, bees, followed by freezing, homogenization with addition of 40% alcohol to the homogenate at the rate of 20 g per 100 ml of alcohol, insisting in a dark place for 13-14 days and pouring into sterile vials, while collecting 9-10-day-old larvae, along with honeycomb wax, honey and perga, while freezing at a temperature of (-16 ° C) - (- 18 ° C), and then without preliminary defrosting I collect homogenize for 30-40 seconds until a homogeneous mass is obtained and infusion is carried out at a temperature of (+ 18 ° C) - (+ 23 ° C).

Thus, the result of increasing the resistance of the dog’s organism can be achieved by containing a unique complex of protein, free amino acids, carbohydrates, minerals, and biologically active substances in the feed additive. So, the composition of 9-10-day-old drones larvae includes a large number of functional groups of enzymes, as well as testosteroid hormones (see "Using drone brood for cosmetic purposes", A.G. Bachinsky, A.N. Decina, J. " Beekeeping ”, No. 5, 1998, p. 54), extraradiol progesterone, and the proteins are mainly free amino acids and easily digestible oligopeptides. 28 amino acids were found in dried drone larvae, including 9 irreplaceable ones. Among the latter, there is a significant amount of lysine, isoleucine, phenylalanine (see "Biological and nutritional value of Bilar powders", A.I. Cherkasov, I.A. Prokhoda, J. "Beekeeping", No. 2, 2005, p.50- 51).

Table 1 shows the chemical composition of the homogenate from 9-10-day drones.

Table 1 Chemical composition Vitamins Trace elements Protein - 10-20%, Vitamin A - 0.083 IU / g Calcium - 3.3 mg% Vitamin B ₂ - 0.114 mg% Magnesium - 16.3 mg% Niacin - 2.43 mg%, Sodium - 34.6 mg% Including free amino acids - 1.0-1.3% Vitamin D - 146 IU / g Potassium - 21.4 mg% Carbohydrates - 1.0-5.5%, And also In ₁ In ₆ , B ₁₂ , C, E, H, folic and pantothenic acids Iron - 3.23 mg% Including glucose - 3.16% Manganese - 0.022 mg% Fructose - 0.03-0.5% Zinc - 0.2 mg% Lipids - 5.0-6.3% As well as Ag, Au, Co, Ni, etc.

The composition of the bee bread, which is one of the components of the feed supplement, includes pollen with the addition of the secretion of the salivary glands and honey, which bees can preserve pollen. Flower pollen is also rich in proteins (11-35%), which are very similar in composition to serum proteins, carbohydrates - glucose, fructose (20-39%). Perga is a source of many macroelements (potassium, calcium, phosphorus, iron, magnesium) and microelements (manganese, chromium, zinc, iodine, etc.). The composition of the bee bread includes vitamins A, C, D, E, B, P, K, phytohormones, flavonoids, hormones (see "Beekeeping products: characteristics, production and processing technology", V.I. Ternova, PM Zlydneva, Stavropol: AGRUS Publishing House, 2004).

Beeswax is a mixture of 300 chemical compounds: esters (70-75%), saturated hydrocarbons (12-15%), free acids (15%), macro- and microelements, vitamin A, pollen, resins, pigments, aromatic substances .

The essence of the method of preparing a feed supplement from drones larvae to increase the resistance of dogs in parasitosis

A feed additive is prepared from drones, waxes and beetroot larvae contained in honeycomb cells.

For this, frames with sushi for drones are placed in the bee colony on the second building. 9-10 days after the uterus inoculated the frames, the completely sealed frames are removed from the hive. A 9-10-day brood of drones is cut along with the honeycombs without printing them, and placed in a freezer at a temperature of (-16 ° C) - (- 18 ° C). Frozen material in small portions (150-200 g) is homogenized with a blender for 30-40 seconds until a homogeneous mass is obtained. The resulting biological mass (9-10-day drones larvae, honey, bee bread and wax) is poured with 40% alcohol at the rate of 20 g of mass per 100 ml of alcohol and insisted in a dark place at a temperature of (+ 18 ° C) - (+ 23 ° C) for 13-14 days, then filtered through 4 layers of sterile gauze and poured into vials.

The implementation of the invention.

Examples of specific performance of the method of preparing a feed additive from drones larvae

Example No. 1. For this, frames with sushi for drones are placed in the bee family on the second building. 9-10 days after the uterus inoculated the frames, the completely sealed frames are removed from the hive. A 9-10-day brood of drones is cut together with the honeycombs, without printing them, and crushed using an electric meat grinder with a mesh hole diameter of 3 mm, and then the resulting mass is poured with 40% alcohol at the rate of 20 g of mass per 100 ml of alcohol and insisted in in a dark place at a temperature of (+ 18 ° С) - (+ 23 ° С) for 10 days, then filter through 4 layers of sterile gauze and pour into vials.

When grinding material in a meat grinder, a significant part of the material remained in the apparatus, clogging it, in addition, it was not possible to achieve the required particle size and uniform consistency.

Example No. 2. For this, with a bee family, frames with sushi for drones are placed on the second building. 9-10 days after the uterus inoculated the frames, the completely sealed frames are removed from the hive. A 9-10-day brood of drones is cut along with the honeycombs, without printing them, and placed in a freezer at a temperature of -18 ° C. The frozen material is crushed using an electric meat grinder with a mesh hole diameter of 3 mm, and then the resulting mass is poured with 40% alcohol based on 20 g of mass per 100 ml of alcohol and insisted in a dark place at a temperature of (+ 18 ° C) - (+ 23 ° C) for 14 days, then filtered through 4 layers of sterile gauze and poured into vials.

When grinding frozen material in a meat grinder, it was also not possible to achieve the required particle sizes and uniform consistency.

Example No. 3. Carried out analogously to example No. 2, but the frozen material in small portions (150-200 g) is homogenized using a blender for 30-40 seconds to obtain a homogeneous mass. The resulting biological mass is poured with 40% alcohol at the rate of 20 g of mass per 100 ml of alcohol and insisted in a dark place at a temperature of (+ 18 ° C) - (+ 23 ° C) for 14 days, then filtered through 4 layers of sterile gauze and bottled and used.

When treating dogs with mixtinvasion, toxocariasis, ankylostomiasis, trichocephaliasis, toxascaridosis, the resulting feed additive contributed to a more rapid gain in animal body weight (Table 2), normalization of red blood cells and hemoglobin in the blood (Table 3, 4, 5), white blood picture (Table 3). .6) and, in general, an increase in the overall resistance of dogs.

Example No. 4. Carried out analogously to example No. 3, but for freezing, put the material in the freezer at a temperature of -16 ° C, and insist on 40% alcohol for 13 days.

The resulting feed additive contributed to increasing the resistance of dogs in the symptomatic treatment of parasitosis, as well as the additive obtained in example No. 3.

Example No. 5. Conduct similarly to experiment No. 3, but insist in a dark place for 20 days.

The resulting feed additive contributed to increasing the resistance of dogs in the symptomatic treatment of parasitosis, but more time is spent on its preparation.

Example No. 6. Carry out analogously to example No. 3, but instead of the larvae of 9-10 days old take 7-8-day larvae.

The resulting biological mass of a thicker consistency than in example No. 3, due to the smaller mass of larvae and more - wax. In addition, when testing the drug from 7-8-day-old larvae, a lower therapeutic effect was noted: weight gain was 20% lower than in the example with 9-10-day-old larvae, the recovery process of hemoglobin and erythrocytes was slower by 4- 5 days.

Example No. 7. Carried out analogously to example No. 3, but pour the mass of 70% alcohol and insist in a dark place for 10 days.

The resulting feed additive when given orally in several experimental animals caused an exacerbation of gastroenteritis.

Thus, examples 3 and 4 are the most optimal, since the feed additive obtained in these examples contributed to faster weight gain of animals, normalization of red blood cells and hemoglobin in the blood, white blood picture, and generally an increase in the overall resistance of dogs, as demonstrated in tables 2-6.

table 2 The dynamics of the live weight of dogs treated against nematodoses using a feed supplement from drones and without Group of experimental animals, drug Options The presence of helminth eggs, weight Before treatment In 12 days In 24 days Weight, kg Helminth eggs Weight, kg Helminth eggs Weight, kg Helminth eggs 1 group n = 3 (Troncil 0.066 g / kg - once) M ± m twenty Toxocara canis, Ancylostoma caninum, Trichocephalus vulpis, Toxocara leonine. 20.07 ± 0.06 - 20.27 ± 0.03 - Group 2 n = 3 (Troncil 0.066 g / kg - once + feed supplement 0.5ml / kg for 10 days) M ± m twenty Toxocara canis, Ancylostoma caninum, Trichocephalus vulpis, Toxocara leonine. 20.3 ± 0.09 - 20.8 ± 0.35 - control group (without treatment) n = 3 M ± m twenty Toxocara canis, Ancylostoma caninum, Trichocephalus vulpis, Toxocara leonine 19.8 ± 0.1 Toxocara canis, Trichocephalus vulpis, Toxocare leonine, Ancylostoma caninum. 19.75 ± 0.05 Toxocara canis, Trichocephalus vulpis, Toxocara leonine, Ancylostoma caninum

Table 3 The number of red blood cells 10 ¹² / _l in dogs treated against nematodoses, using a feed additive from drones and without drones Study time Options 1 group n = 3 (Troncil 0.066 g / 1 kg - once) Group 2 n = 3 (Troncil 0.066 g / kg once + feed supplement 0.5 ml / kg for 10 days) control group (without treatment) n = 3 Before experience M ± m 4.7 ± 0.3 4.3 ± 0.4 4.6 ± 0.35 In 12 days M ± m 6 ± 0.5 8.3 ± 0.2 4,5 ± 0,2 In 24 days M ± m 7.16 ± 0.4 8.4 ± 0.3 2.1 ± 0.2

Table 4 The number of leukocytes in dogs treated against nematodoses using a feed supplement from drones and without Study time Options 1 group n = 3 (Troncil 0.066 g / 1 kg - once) Group 2 n = 3 (Troncil 0.066 g / kg once + feed supplement 0.5 ml / kg for 10 days) 3 control group (without treatment) n = 3 Before experience M ± m 15.9 ± 0.8 16.7 ± 0.5 18 ± 0.8 δ 1.5 0.9 1.5 In 12 days M ± m 11.6 ± 0.4 10 ± 0.5 22.8 ± 0.1 δ 0.7 0.9 0.2 In 24 days M ± m 8.7 ± 0.17 9.16 ± 0.2 22.8 ± 0.1 σ 0.3 0.4 0.2

Table 5 The amount of hemoglobin g / l in dogs treated against nematodoses using a feed additive from drones and without drones Study time Options 1 group n = 3 (Troncil 0.066 g / 1 kg - once) Group 2 n = 3 (Troncil 0.066 g / kg once + feed supplement 0.5 ml / kg for 10 days) control group (without treatment) n = 3 Before experience M ± m 89.6 ± 0.6 89 ± 0.9 82.3 ± 1.4 In 12 days M ± m 115 ± 2.8 150 ± 2.8 90.5 ± 1.5 In 24 days M ± m 115 ± 2.8 138.3 ± 1.6 67.5 ± 2.5

The invention in comparison with the prototype and other known solutions has the following advantages:

- the feed additive has a high effect in the complex treatment of dogs with parasitosis;

- increases the general specific resistance of an organism;

- the proposed method allows you to completely save the nutrient and biologically active substances contained in the cells of cells (drone milk, biological mass of larvae, bee bread and wax).

- the proposed manufacturing method is simple and economical.

Claims (1)

A method of preparing a feed additive from drones larvae to increase the resistance of dogs in case of parasitosis, including collecting drones larvae, beef, followed by freezing, homogenization with addition of 40% alcohol to the homogenate at the rate of 20 g per 100 ml of alcohol, insisting in a dark place in for 13-14 days, followed by straining and pouring into sterile vials, characterized in that they collect 9-10-day-old larvae along with honeycomb wax, honey and perga, freezing at a temperature of (-16) - (- 18) ° С and then without n edvaritelnogo defrost charge was homogenized in a blender for 30-40 seconds to obtain a homogeneous mass and maceration is carried out at a temperature of 18-23 ° C.