RU2435433C1 - Method for production of lyophilised biologically active dietary supplement for animals and compound based thereon - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to veterinary, in particular - to a method for preparation of a biologically active dietary supplement and a compound based thereon consisting of natural components. The method for preparation of the lyophilised biologically active dietary supplement for animals envisages collection of 9-10 days' drone larvae, their freezing, storage till usage with subsequent homogenisation; the method specificity consists in additional usage of pumpkin and distilled water. Pumpkin is peeled, cut into small pieces, frozen at a temperature of -(18-20)°C during 22-24 hours, defrosted to a temperature of +(17-18)°C during 9-11 minutes, homogenised with subsequent blending with drone larvae homogenate; drone larvae freezing proceeds at a temperature of -(18-20)°C while homogenisation of drone larvae and pumpkin is performed till particle size is 0.5-1.0 mm. Then, prior to packaging, the homogenate is mixed at ratio of drone larvae to pumpkin and distilled water 1:4:5; then one proceeds with lyophilic drying and sublimation, observing specific parameters of these operations performance. The compound for preparation of the lyophilised biologically active dietary supplement for animals contains drone larvae homogenate -10 wt %, pumpkin homogenate - 40 wt % and distilled water - balance.

EFFECT: group of invention enabling increase of animal body weight, enhancement of their general and specific resistance, male and female reproductive function, prevention of allergic reactions and reduction of manufacture costs.

2 cl, 8 tbl, 6 ex

Description

The technical field to which the invention relates ..

The invention relates to veterinary medicine, in particular to methods for preparing a biologically active additive and a tool based on it from natural components: drones and pumpkin larvae, and can be used to increase the general and specific resistance of the organism and the reproductive function of animals.

The prior art.

There is a method of preparing a drug for stimulating the human body from drones larvae, including collecting drones larvae, their homogenization with the addition of a stabilizing medium (see. "Harvesting drones larvae" V.I. Lebedev, M.A. Logovich, Research Institute of beekeeping, J. "Beekeeping ", No. 3, 2003, p. 52-54).

The disadvantage of this method is the short storage time of the drug.

A known method and means of improving the health of athletes, including eleutherococcus extract, characterized in that it additionally contains licorice root extract dry, rosehip dry, estifanum, silymar, propolis tincture, natural honey, and as an eleutherococcus extract, dry eleutherococcus extract in the following ratios of components, wt.%: dry eleutherococcus extract - 0.6; dry licorice root extract - 0.3; dry rosehip extract - 0.3; estifan - 0.3; silymar - 0.3; propolis tincture - 0.3; natural honey. Take in the pre-competitive and competitive periods for 7-10 days, 1 teaspoon diluted in 0.5 cups of water, 2-3 times a day, only 150-200 g per course, the use of the drug is repeated 2-3 courses with an interval of 7 -10 days (see US Pat. RF No. 2104013, class A61K 35/64, A61P 35/78, publ. 12/26/1995).

The disadvantage of this method is the complicated method of preparation, expensive composition and course of application.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is a method of preparing a preparation for stimulating the organism of animals from drones larvae, including collecting drones larvae, homogenizing them with the addition of a stabilizing medium, and when homogenizing drones, they are frozen at a temperature of (-4 ; -5) ° C for 12-14 hours, then thawed for 3-5 minutes to + 18 ° C, while a stabilizing medium using a 5% glucose solution in a ratio of 1:10 5% solution of chloroform or 2% salicylic acid, followed by sublimation, and sublimation is carried out in a vacuum chamber at a temperature of heating the shelves to (+ 30-45) ° С, while achieving the temperature of the drug (+ 20-22) ° C, the temperature of the shelves is reduced to + 25 ° C and drying is carried out for 22-24 hours (see US Pat. RF No. 2258522, class A61K 35/64, A61P 37/04, publ. 20.08.2005).

The disadvantage of this method is that the drug obtained by this method is administered intramuscularly, causes a local reaction of the body, has low stimulating properties.

Disclosure of invention

The objective of the invention is to develop a method for the preparation of a biologically active additive (BAA) from larvae of drones and pumpkins and a tool based on it, with which the prepared BAA has a high stimulating effect, increasing the general and specific resistance and reproductive function of animals, eliminating allergic reactions, and more cheap to manufacture.

The technical result, which can be obtained using the present invention, is to increase the weight of the animals, the general and specific resistance, reproductive function of females and males, the exclusion of allergic reactions, cheaper manufacturing.

The technical result is achieved using a method of preparing a biologically active additive for animals, including 9-10-day drones larvae, their freezing, storage until use, followed by homogenization and packaging, while it additionally contains pumpkin and distilled water, while the pumpkin is pre-cleaned, cut into small pieces, frozen at a temperature of - (18-20) ° C for 22-24 hours, then thawed to a temperature of + (17-18) ° C for 9-11 minutes, homogenized, followed by compound with a homogenate m of drones larvae, moreover, drones larvae are frozen at a temperature of - (18-20) ° С, and drones and pumpkin larvae are homogenized to particle sizes of 0.5-1.0 mm, while homogenate is mixed before packing in the ratio of 1 part drones larvae, 4 parts pumpkin and 5 parts distilled water, followed by freeze drying and sublimation, and freeze drying is carried out before freezing, at least with a 5-shelf shelf, pre-cooled to - (44-4.5) ° C for 13-14 hours followed by gradual m increase in temperature during freeze drying by 5 ° С from (+10) ° С to (+30) ° С, and when the product temperature reaches + 22 ° С, the temperature of the shelves is reduced to + 25 ° С and after reaching the temperature in the product 22- At 26 ° C, drying is carried out for 44-45 hours, and sublimation is carried out in vacuum ≥8 mV deep with a total drying time of 93-94 hours.

The technical result is achieved using a means of preparing a lyophilized biologically active additive for animals, including a homogenate of drones, pumpkin homogenate and distilled water in the following ratio of components in wt.%:

drone larvae homogenate 10.0 pumpkin homogenate 40,0 distilled water rest.

The essence of the method of preparation of lyophilized biologically active additives for animals and means based on it is as follows.

To obtain 9-10-day drones larvae during the rapid development of bee colonies, 2 frames for a drone brood are placed in each hive and observed when they are sown. On the 9-10th day after the bees seal the frames with brood, they are taken from the hive. Convex caps are cut from cells with a drone brood using a knife for printing honeycombs. In a baking sheet, the size suitable for the size of the frame, drones larvae are knocked out together with drone milk and collected in plastic containers of 0.5 liter capacity. Then it is frozen in a freezer at a temperature of - (18-20) ° С and stored for 2-3 months before use. Before use, they are thawed to + (17-18) ° C in the microwave for (9-10) minutes and homogenized in a blender for 1 minute at 3 thousand revolutions.

Ripe pumpkin is harvested, washed, rinsed with cool boiling water, peeled, cut into small pieces, frozen at - (18-20) ° C for 22-24 hours, then thawed to + (17-18) ° C for (9-11) min in a microwave oven and homogenized in a blender for 1 minute at 3 thousand revolutions until a homogeneous mass is formed (particle size 0.5-1.0 mm). Then combine the volume of 1: 4: 5 (1 part of larvae, 4 parts of pumpkin with the addition of 5 parts of distilled water) in the following ratio, wt.%:

drone larvae homogenate 10.0 pumpkin homogenate 40,0 distilled water rest,

Packed in 100 ml sterile glass bottles and subjected to freeze drying, which is carried out under a special regime: with pre-freezing in the HC 700/50 refrigerator with a 5-shelf shelf, pre-cooled to - (44-45) ° С. Freezing is carried out at - (44-45) ° C for 13-14 hours. Sublimation is carried out after overloading the bookcase with bottles into the sublimation chamber of an installation of type LZ-45 No. 7, after connecting sensors and connectors that control the temperature of the product and shelves, vacuum and evaporator readings. A vacuum is created within an hour (≥8 mV depth) and the shelf heating temperature is set at + 10 ° C and heating is turned on. Then, every 5 hours, the temperature of the shelves is increased by 5 ° C to + 30 ° C. When the product temperature reaches + 22 ° С, the temperature of shelf heating decreases to + 25 ° С. After reaching a temperature in the product of 22-26 ° C, drying is done within 45 hours. The total drying time is 93-94 hours. After drying, the bottles with the drug are corked with stoppers and rolled in caps.

The implementation of the invention

Examples of a specific implementation of the method of preparing a lyophilized dietary supplement for animals and an agent based on it.

Example No. 1

Drones larvae are specially grown up to 9-10 days of age during the rapid development of bee colonies. Drone brood frames are taken from families. With a knife for printing honeycombs, convex wax caps are cut off and beaten onto a baking sheet. Then the obtained material (larvae + drone milk) is collected in sterile plastic or glass containers, sealed and frozen at -20 ° C and stored for 2-3 months until use. Before use, they are thawed to + 18 ° C in a microwave for 10 minutes and homogenized in a blender for 1 minute at 3 thousand revolutions.

The homogenate of drone larvae and distilled water are combined in a ratio of components (1 part of larvae, 9 parts of water), wt.%:

drone larvae homogenate 10.0 distilled water rest

and sterile 50 ml vials are poured into 100 ml and first subjected to freezing in a HC 700/50 refrigerator with a 5-shelf shelf, pre-cooled to -44 ° C. Freezing is carried out at -44 ° C for 13 hours. Sublimation is carried out after reloading the bookcase with bottles into the sublimation chamber of an installation of type LZ-45 No. 7, after connecting the sensors and connectors and creating a vacuum (≥8 mV deep), the shelf heating temperature is set at + 10 ° C and heating is turned on. Then, every 5 hours, the temperature of the shelves is increased by 5 ° C to + 30 ° C. When the product temperature reaches + 22 ° С, the temperature of shelf heating decreases to + 25 ° С. After reaching a temperature in the product of 22-26 ° C, drying is done within 44 hours. The total drying time was 93 hours. After drying, the bottles with the drug are corked with stoppers and rolled in caps.

The resulting preparation is a loose brown mass that dissolves in water. It maintains the established shelf life of 2 years. But when used, it does not give the expected effect of increasing resistance and reproductive function (see Table 1).

Example No. 2

Carried out analogously to example 1, but to the homogenate of the drone larvae add pumpkin homogenate and distilled water.

Ripe pumpkin is harvested, washed, rinsed with cool boiling water, peeled, cut into small pieces, frozen at -18 ° C for 22 hours, then thawed in a microwave oven to + 18 ° C for 9 min and homogenized in a blender 1 minute at 3 thousand revolutions until a homogeneous mass is formed (particle size 0.5-1.0 mm). The homogenate of drones, pumpkin larvae and distilled water are combined in the following ratio of components (0.3 parts of drones, 2 parts of pumpkins and 7.7 parts of water), wt.%:

drones larvae homogenate 3.0 pumpkin homogenate 20,0 distilled water rest

and 50 ml sterile vials are poured into 100 ml.

Drying mode is the same as in the 1st example.

The resulting preparation is a loose light brown mass that dissolves in water. He retains his color for 2 years. But when used, it does not give the expected effect of increasing resistance and reproductive function (see Table 2).

Example No. 3

Carried out analogously to example 2, but the components are mixed in the following ratio (0.5 parts of drones larvae, 3 parts of pumpkin and 6.5 parts of water), wt.%:

drone larvae homogenate 5,0 pumpkin homogenate 30,0 distilled water rest.

The drying mode was the same as in the 1st example.

The resulting preparation is a loose light brown mass that dissolves in water. He retains his color for 2 years. When used, it gives a fairly good expected effect of increasing resistance and reproductive function (see table 3).

Example No. 4

Drones larvae are specially grown up to 9-10 days of age during the rapid development of bee colonies. Drone brood frames are taken from families. With a knife for printing honeycombs, convex wax caps are cut off and beaten onto a baking sheet. Then the obtained material (larvae + drone milk) is collected in sterile plastic or glass containers, sealed and frozen at -18 ° C and stored for 2-3 months until use. Before use, they are thawed to + 17 ° C in a microwave for 9 minutes and homogenized in a blender for 1 minute at 3 thousand revolutions.

Ripe pumpkin is harvested, washed, rinsed with boiling water, peeled, cut into small pieces, frozen at -20 ° C for 24 hours, then thawed in a microwave oven to + 17 ° C for 11 minutes and homogenized in a blender 1 minute at 3 thousand revolutions until a homogeneous mass is formed (particle size 0.5-1.0 mm). The homogenate of drones, pumpkin larvae and distilled water are combined in the following ratio of components (1 part of drones larvae, 4 parts of pumpkin and 5 parts of water), wt.%:

drones larvae homogenate 10.0 pumpkin homogenate 40,0 distilled water rest,

and sterile 50 ml vials are poured into 100 ml and first subjected to freezing in a HC 700/50 refrigerator with a 5-shelf shelf, pre-cooled to -45 ° C. Freezing is carried out at -45 ° C for 13 hours. Sublimation is carried out after reloading the bookcase with bottles into the sublimation chamber of an installation of type LZ-45 No. 7, after connecting the sensors and connectors and creating a vacuum (≥8 mV deep), the shelf heating temperature is set at + 10 ° C and heating is turned on. Then, every 5 hours, the temperature of the shelves is increased by 5 ° C to + 30 ° C. When the product temperature reaches + 22 ° С, the temperature of shelf heating decreases to + 25 ° С. After reaching a temperature in the product of 22-26 ° C, drying is done within 45 hours. The total drying time was 94 hours. After drying, the bottles with the drug are corked with stoppers and rolled in caps.

The resulting preparation is a loose brown mass that dissolves in water. It maintains the established shelf life of 2 years. When used, it gives the high expected effect of increasing resistance and reproductive function (see tables 4, 5).

Example No. 5

Carried out analogously to example 2, but the components are mixed in the following ratio (1 part of the larvae of drones, 4 parts of pumpkin and 5 parts of water), wt.%:

drone larvae homogenate 10.0 pumpkin homogenate 40,0 distilled water rest.

The drying mode was the same as in the 1st example.

The resulting preparation is a loose light brown mass that dissolves in water. He retains his color for 2 years. When used, it gives the high expected effect of increasing resistance and reproductive function (see tables 6, 7).

Example No. 6

Carried out analogously to example 2, but the components were mixed in the following ratio (2 parts of drone larvae, 5 parts of pumpkin and 3 parts of water), wt.%:

drone larvae homogenate 20,0 pumpkin homogenate 50,0 distilled water rest.

Drying mode is the same as in the 1st example.

The resulting preparation is a loose light brown mass that dissolves in water. He retains his color for 2 years. When used, it gives the same high expected effect of increasing resistance and reproductive function as in the 5th example (see table 8). But at cost it comes out much more expensive.

Thus, examples 4 and 5 turned out to be the most optimal. The dietary supplement obtained by this method is a loose light brown mass in the form of a tablet that dissolves in water, the animals are given inside, and it is kept for 2 years. When tested on animals, weight increased, the number of red blood cells increased, brightly flowing conditioned and unconditioned reflexes appeared, libido increased, sperm quality and reproductive function of females improved by 10-15%.

The invention in comparison with the prototype and other known technical solutions has the following advantages:

- the drug increases the general and specific resistance of the animal organism;

- the drug improves the reproductive function of males and females;

- cheaper to manufacture;

- does not have allergic effects;

- introduced inside;

- has a shelf life of 2 years.

Table 4 The content of vitamins and chemical elements in pumpkin Dry matter 11.9-13.2% Total sugar 7.7-8.1% Heavy metal salts Not found Macronutrients mg Potassium 170 Calcium 40 Magnesium fourteen Sodium fourteen Phosphorus 25 Vitamins mg Trace elements mcg Carotene 1,5-7,1 iron 800 Vitamin B1 0.05 cobalt one Vitamin B2 0.06 manganese 114 Vitamin B5 0.4 copper 170-370 Vitamin B6 0.13 molybdenum one Vitamin BC 14.9 fluorine eighteen Vitamin C 8-15 zinc eighteen Vitamin PP 0.5 lead 0,025

Table 5 Composition of drones % 100 g of larvae contains mcg Fat 22.2 Cobalt 2.5 glycogen 24.5 Magnesium fourteen nitrogen 6.2 Copper one Molybdenum four Nickel 7 Bora All interchangeable and irreplaceable amino acids, the largest proportion mg% Aspartic acid 48,4 Glycine 49.1 Tyrosine 52.9 Arginine 26.1 Lysine 23.6 Proline 21,4

Claims (2)

1. A method of preparing a lyophilized dietary supplement for animals, including collecting 9-10-day-old drones larvae, freezing them, storing them until use, followed by homogenization and packaging, characterized in that it additionally uses pumpkin and distilled water, with the pumpkin being preliminarily cleaned, cut into small pieces, frozen at a temperature of - (18-20) ° C for 22-24 hours, then thawed to a temperature of 17-18 ° C for 9-11 minutes, homogenized, followed by combining with a homogenate of t in the morning, moreover, drones larvae are frozen at a temperature of - (18-20) ° C, and drones and pumpkins are homogenized to particle sizes of 0.5-1.0 mm, and before packing, homogenate is mixed in the ratio of 1 part of drones , 4 parts of pumpkin and 5 parts of distilled water, followed by freeze drying and sublimation, and before freeze drying, freezing is carried out with at least a 5-shelf shelf, pre-cooled to - (44-45) ° С for 13-14 h followed by a gradual increase in those the temperature during freeze drying at 5 ° C from 10 ° C to 30 ° C, and when the product temperature reaches 22 ° C, the temperature of the shelves is reduced to 25 ° C and after reaching the temperature in the product 22-26 ° C, drying is carried out for 44 -45 hours, and sublimation is carried out in vacuum with a depth of ≥8 mV with a total drying time of 93-94 hours. 2. A means of preparing a lyophilized dietary supplement for animals, including a homogenate of drones, characterized in that it further comprises pumpkin homogenate and distilled water in the following ratio, wt.%:
drone larvae homogenate 10.0 pumpkin homogenate 40,0 distilled water rest