RU2538085C2 - Agent for control of exo-parasites of animals - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: agent for control of exo-parasites of animals comprises the active substances and water, and the active substances are used as fish oil and nonionic surfactant with the following ratio of components: fish oil - 20 g; nonionic surfactant - 85 g; water - 10 ml.

EFFECT: reduction of cost of the agent and its rate of consumption, reduction of labour cost in its production and expanding the range of action.

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Description

The invention relates to the field of veterinary medicine, in particular to means for treating animals, and can be used for the prevention and treatment of diseases caused by exoparasites of animals.

Known drug for combating animal exoparasites, containing active substances and water, and the drug additionally contains rosin or a mixture of rosin and tall pitch in a ratio of 1.0: 0.8 in the following ratios of components, wt.%: Permethrin 15-25, oil polymer 45-55, rosin or a mixture of rosin and tall pitch in the ratio of 1.0; 0.8 25-27.5, water - the rest (see RF patent No. 1672610, IPC A01N 43/00, publ. 03/27/1995).

Known drug for the control of exoparasites of animal roads, its use is associated with the repetition of a course of treatment, but even repeated treatments do not always provide a relapse-free treatment. The drug has a toxic effect.

The closest in technical essence is a preparation for combating animal exoparasites, containing active substances and water, moreover, liquid insecticidal soap from rapeseed oil and Artemia sp. Hydrolyzate are used as active substances. in the following ratio of components, wt.%: liquid insecticidal soap 0.15-0.45; hydrolyzate Artemia sp. 0.15-0.45; the rest is water (see RF patent No. 2426534, IPC A01N 43/00, publ., 08/20/2011),

A disadvantage of the known drug is its high cost and high consumption rate, as well as the complexity of its preparation, associated with the need for preliminary production of the components used for the manufacture of the drug (insecticidal soap and Artemia sp. Hydrolyzate), as well as an insufficiently wide spectrum of its action against certain diseases , for example, with respect to rabbit nosedrosis caused by Notoedres cuniculi percutaneous tick.

The technical result of the invention is to reduce the cost of the drug and its consumption rate, as well as reducing labor costs in its production and expanding the spectrum of action.

The technical result is achieved in that in a preparation for controlling animal exoparasites containing an active substance and water, according to the invention, fish oil and a nonionic surfactant are used as the active substance in the following ratio of components:

fish fat 20 g nonionic surfactant 85 g water 10 ml

The preparation and its use is illustrated by the example of a specific implementation.

Example. 85 g of a nonionic surfactant, 20 g of fish oil and 10 ml of water are placed in the capacity of a tissue grinder RT-1 equipped with a propeller stirrer.

Then turn on the stirrer, and the mixture is stirred for 10-12 minutes. Get the claimed drug in the form of a liquid creamy white mass in the amount of 115 g.

To obtain a working solution in 1 liter of water, 40 g of the proposed drug is dissolved and thoroughly mixed. Thus, a 4% working solution for treating rabbits for psoroptosis is obtained by injecting 3 ml in each ear. From the obtained amount of the preparation (115 g), 2.875 L of a 4% working solution can be prepared for the treatment of about 500 rabbits.

Tests were preliminarily conducted in laboratory conditions, where different concentrations of drugs were tested. For experiments, 150 isolated ticks Psoroptes cuniculi and Notoedres cuniculi were taken from freshly collected scrapings in the lesions of experimental rabbits, in the first case scrapings were taken from the auricles, and in the second case they were taken from the front of the head, mainly the nose and eyelids. As a reference, a well-known prototype preparation was used (RF patent No. 2426534).

The results of laboratory tests showed that 100% death of Psoroptes cuniculi ticks in a known preparation (standard) was observed only at a concentration of 8%, and the proposed preparation provided 100% death at a concentration of 4%, which is twice lower than in the standard (table one).

Table 1 The test results of the claimed drug against rabbit psoroptosis A drug Concentration Number of ticks (Psoroptes cuniculi) % Death taken for experience actively moving paralyzed the dead Reference four thirty 0 ⁰ 24 80.0 Reference 8 thirty 0 0 thirty one hundred The inventive drug four thirty 0 0 thirty one hundred The inventive drug 8 thirty 0 0 thirty one hundred Control - (distilled water) - thirty thirty 0 0 0

Against ticks of Notoedres cuniculi, the effectiveness of the known drug (prototype) does not exceed 83.3% even at a maximum concentration of 8%, while the proposed drug caused 100% death of this type of ticks at a drug concentration of 4%, which is twice lower than in known drug prototype (table 2).

table 2 The test results of the claimed drug against nootrosis of rabbits A drug Concentration Tick Number (Notoedres cuniculi) % Death taken for experience actively moving paralyzed the dead Reference four thirty four 16 10 33.3 The inventive drug 8 thirty one fourteen 25 83.3 The inventive drug four thirty 0 0 thirty one hundred The inventive drug 8 thirty 0 0 thirty one hundred Control - (distilled water) - thirty thirty 0 0 0

The higher acaricidal activity of the proposed drug is due to the fact that its mechanism of action is based on blockage of spiracle mites with tiny drops of fish oil with the subsequent effect of hypoxia, i.e. ticks died from suffocation.

After laboratory experiments, tests were conducted on experimental animals. To study the therapeutic efficacy of the proposed drug, California rabbits were taken with a live weight of 1.5-4.5 kg. In each of the five experimental groups were 10 animals. In all experimental animals, peoroptosis or notedrosis, or mixed invasion of varying severity (mainly moderate), was clinically established. The diagnosis was confirmed by microscopy of scrapings of the skin of the auricles during psoroptosis and scrapings from the front of the head, mainly the nose and eyelids (with notedrosis).

Each experimental group was treated once with a working solution of drugs at the rate of 2-3 ml in each auricle of a rabbit with psoroptosis or by rubbing the drug with a sponge of the face of the head, mainly of the nose and eyelids (with knotrosis).

Based on the analysis of the results obtained, it was found that after treatment with the 4% and 8% working solution of the proposed drug, all experimental animals recovered, there was no clinical manifestation of psoroptosis, no live ticks, larvae, and other developmental phases were found in brackets, as in Psoroptes cuniculi, and according to Notoedres cuniculi, while the well-known drug (prototype) provided complete recovery of animals from psoroptosis only at a concentration of 8%, and showed insufficient effectiveness against nootedrosis.

Thus, the test results of the proposed drug in experimental rabbits fully confirmed the results of laboratory experiments. The therapeutic efficacy of the drug at a concentration of 4% and 8% was 100% both against psoroptosis and against notedrosis.

Within 30 days (study period) side effects indicating a negative effect of the proposed drug on the animal organism were not observed. Moreover, when treating experimental animals with the proposed drug, faster removal of crusts and faster healing of affected tissues was noted, which is obviously due to the fact that fish oil, which is part of the proposed drug, has an active effect on metabolic processes in animal tissues.

The composition that differs in composition from the proposed boundary values of the ratio of components does not cause recovery in the tested groups of rabbits.

The claimed drug is effective against other exoparasites, for example against the ixodid tick Dermacentor concin and Menacanthus stramineus Nitzsch. - a parasite of chickens (downy-eater).

Below are the results of laboratory experiments in comparison with the well-known drug neostomazan, which is a highly toxic drug (tables 3 and 4).

Table 3 The effectiveness of the claimed drug against ixodid tick Dermacentor concin (laboratory experience) A drug Concentration% The death of ticks,% when immersed in a working solution when processing foam Control - no processing - 2.0 - Neostomazan 0.001 one hundred - The inventive drug 2 85.7 one hundred The inventive drug four one hundred one hundred

The results of laboratory experiments were confirmed when processing cattle against ixodid ticks in the field. Tests were conducted in the Maiminsky district of the Altai Republic.

Table 4 The effectiveness of the drug against the fluffer Menacanthus stramineus Nitzsch parasitizing on chickens (laboratory experience) A drug Concentration% The death of the lopped eater,% Control - no processing - 0.1 Neostomazan 0.001 one hundred The inventive drug 0.25 one hundred The inventive drug 0,025 one hundred The inventive drug 0.005 one hundred The inventive drug 0.001 one hundred The inventive drug 0,0005 one hundred

The results of laboratory experiments showed that the claimed drug is effective against a fluff-eater in a very low dosage (0.0005%). which is 2 times lower in comparison with the chemical drug neostomazane, which is recommended for use at a concentration of 0.001%.

The results of laboratory experiments were tested on live birds in a production environment at the Blagoveshchenskaya poultry farm in the Altai Territory. These tests are presented in table 5.

Table 5 Field test results Option Breed, number of goals Concentration The death of the lopped eater,% Control - no processing Xaisex brown-3 - - The inventive drug Xaisex brown-3 0.25 one hundred

The inventive preparation showed a 100% death of a fluff-eater under production conditions and did not have a negative effect on chickens. During processing, they behaved calmly; no gluing of the pen was observed.

On the body of chickens, dead adult poohoedoids were noted. During 1.5 months of observation, live chickens were not observed on experimental chickens, which indicates an ovicidal effect, i.e. the drug destroys not only adult lop-eater, but also their eggs.

A blood test of experimental and control chickens did not show changes in its composition. Violation of the laying of eggs after treatment with the proposed drug was not observed. The product is environmentally friendly, eggs and poultry are environmentally friendly products. The drug recommended to date, neostomazan is a highly toxic drug, and small deviations from the recommended concentration in the direction of increasing it can lead to the death of the entire population, which has been repeatedly confirmed in practice.

Processing of animals through a working solution of the claimed preparation provides faster recovery due to the activation of metabolic processes in damaged tissues associated with the content of fish oil in the preparation. With all selectively conducted tests of the claimed drug in animals and birds, positive results were obtained with one hundred percent recovery. Therefore, we can conclude that the claimed drug is effective for combating other exoparasites of animals and birds.

The proposed drug has a lower consumption rate and a wider spectrum of action, and the drug is obtained from the available components, which are purchased ready-made, thereby greatly simplifying the production technology of the drug, which reduces labor and money.

The proposed drug is safe and its use provides environmentally friendly products.

Claims (1)

A preparation for combating animal exoparasites, containing active substances and water, characterized in that fish oil and nonionic surfactants are used as active substances in the following ratio of components:
fish fat 20 g nonionic surfactant 85 g water 10 ml