RU2491945C1 - Wound healing agent - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to pharmaceutical industry and medicine, and represents a wound healing agent containing a concentrated raw material from antler-producing deers, differing by the fact that the raw material represents a concentrate of deer's velvet antlers and a concentrate of mixed wild chamomile, birch buds and common clotbur taken in ratio 1:4:5 respectively, extracted and dried in ratio of the mixture of herbal ingredients: water 1:20, with the agent additionally containing stearic acid, glycerol monostearate, bee wax, cosmetic wax, lanolin, vegetable oil, glycerol, sodium tetraborate, triethanolamine, phenochem, polyethylsiloxane liquid, cyclomethicone, an antifoam additive, lidocaine + a flavour, water, with the ingredients of the agent taken in certain proportions, wt %.

EFFECT: invention provides high wound healing action, and long shelf life.

2 cl, 3 tbl

Description

The invention relates to perfume and cosmetics, pharmaceutical industry and medicine, namely to a wound healing drug prepared from raw materials of animal and vegetable origin.

Known wound healing drug for external consumption on the basis of a concentrate of antler deer blood - pantogematogen "dry" including, wt.%: Concentrate - pantogematogen "dry" - 0.01-6.0, polymer gelling - rare crosslinked polymer of acrylic acid 1-6 , carbomer 2.1-7.5, glycerol and / or propylene glycol 2-60, preservatives nipagin and nipazole 0.05-0.4, triethanolamine to a pH of 6-8, water - the rest. The preferred area of application is the treatment of diseases where alcohol-containing forms are contraindicated: burns of varying degrees, skin damage, etc. (RF Patent No. 2288727, IPC A61K 35/14, A61P1).

However, preservatives such as nipagin and nipazole have a narrow spectrum of antimicrobial activity, so nipagin mainly inhibits the development of molds, and nipazole is yeast-like, while antler raw materials, due to their rich biochemical composition, are a good substrate for a wide range of microorganisms, which which the drug does not possess proper preservation, and therefore, medicinal properties.

It is known medicinal wound healing agent based on a concentrate from the uterus of pregnant females with embryos and amniotic fluid, a formative substance - petrolatum and preservative of citrosept, in the following ratio of components, concentrate - 20, citrosept - 0.06-0.1 per 100 g of petroleum jelly (Decision on the grant of a patent of the Russian Federation by application No. 201042130 dated October 13, 2010 - a prototype).

However, the use of a concentrate from the uterus with an embryo and amniotic fluid (which is a unique natural complex containing stem cells, growth factors, proteins, nucleic acids, polysaccharides, lipids, enzymes, amino acids, vitamins and trace elements) in such a concentration (20%) in the composition of the drug means predetermines the not entirely rational use of this unique tool, while medicinal herbs used to heal wound surfaces, in combination with the above concentrate Whether to provide, when combined properties of both, a synergistic wound-healing effect, with more economy consumption concentrate.

Known biologically active concentrate from antlers of deer obtained by enzymatic hydrolysis of antlers in the presence of water and homogenate of the animal’s pancreas at the rate of 100 g of gland per 1 kg of antler flour, followed by aqueous extraction of the hydrolyzate, centrifugation and filtration, followed by drying of the target product at a temperature of no higher than 50 ° C and a pressure of 0.9 atm (Patent RU 2386443, IPC A61K 35/32).

The objective of the invention is to develop a wound healing drug, expanding the arsenal of drugs based on antler products and allowing more efficient use of valuable raw antler reindeer husbandry when using herbal medicines and high wound healing effect of the finished product and a long shelf life.

The specified technical result is achieved by the fact that the wound healing drug includes a concentrate of deer antlers, a concentrate from a mixture of pharmacy chamomile, birch buds, common cockroach taken in a ratio of 1: 4: 5, respectively, and extracted at a temperature of 55-60 ° C for 2, 0-2.5 hours to an extract density of 5.0-5.5% in the ratio of a mixture of plant components: water 1:20, followed by drying of the extract at a temperature not exceeding 40 ° C and a vacuum of 0.8 to 1.0 atm to humidity 6 - 8%, while the product additionally contains, ma .%: stearic acid - 5.0%, glycerol monostearate - 5.5%, beeswax - 0.3%, cosmetic wax - 0.3%, lanolin - 1.0%, vegetable oil - 6.0%, glycerol - 3.0%, sodium tetraborate - 0.3%, triethanolamine - 0.6%, phenochene - 0.3%, polyethylsiloxane liquid - 0.5%, cyclomethicone - 1.5%, antifoam - 0.19% , lidocaine and perfume - 0.52%, water - the rest. The content of each concentrate in the product is 1.5%.

Pharmaceutical camomile. Inflorescences (baskets) of chamomile contain essential oil (0.05-1.0%), up to 11 substances of flavonoid nature, coumarins, organic acids, vitamin C, vitamin B, β-carotene, tocopherol, vitamin K and others. Apigenin - 7 - glucoside, quartzimetrin. Some coumarins and sesquiterpenoids contained in chamomile have antispasmodic activity. The anti-inflammatory and anti-allergic effect of chamomile is associated with the presence of sesquiterpenoids matrixil, chamazulin, bizabolol and some other compounds. Chamomile broth is used to wash suppurating wounds, abscesses. Chamomile preparations are used as anti-inflammatory and deodorizing agents for inflammatory diseases of the skin, oral cavity in the form of rinses, rinses and compresses for dermatoses and trophic ulcers.

Birch buds contain 3.0-5.3% of essential oil. Its main components are sesquiterpene lactones. The kidneys are rich in tarry substances, tannins, contain flavonoids, vitamins, volatile, saponins. The kidneys are used in the treatment of wounds, ulcers, bedsores, eczema, as they have a disinfectant and anti-inflammatory effect. In cosmetics, a decoction of the kidneys is used in the treatment of irritation, itching, and dermatitis.

The common gingerbread contains some alkoloids, a large amount of iodine, ascorbic acid, resins, fatty oils, xanthostrumarine and other active chemical compounds. Decoction of the fruit of the fruit bush is used as an astringent for diarrhea of inflammatory and infectious etiology. Grass juice is used in the treatment of rosacea and acne vulgaris, dermatomycosis, lichen, scrofula, juice relieves itching with insect bites, scabies, allergies. For many skin diseases of a fungal nature, it is recommended to apply fresh leaves to the affected areas or make lotions from a decoction of the grass.

Antler deer concentrate (TU 9860-010-29734071-11 "Soluble concentrate from antler deer products" Concept ") is a unique natural complex containing stem cells, growth factors, proteins, lipids, enzymes, amino acids, vitamins and macro, microelements . Growth factors, like hormones, have a wide range of biological effects on many cells of the body - they stimulate or inhibit mitogenesis, chemotaxis and cell differentiation (maturation). So, cells divide under the influence of insulin-like (IGF) and epidermal (EGF) growth factors, and they mature with the help of transforming (TGF) growth factor, due to which the number of cell division increases, directionally stimulating metabolism in them and promotes rapid wound healing.

Stearic acid and glycerol monostearate is an emulsifier, stabilizer, forms a cream structure and creates a uniform emulsion, preventing the delamination of the components of the product.

Beeswax and cosmetic wax acts as an emollient, and also has a nourishing and anti-inflammatory effect.

Lanolin is the foundation of the cream and has a nourishing effect on the skin.

Triethanolamine is a pH regulator.

Vegetable oil is the fatty basis for dissolving the components of substances.

Glycerin helps moisturize and nourish tissues.

Preservatives: sodium tetraborate, blocks the development of molds, phenochen has a wide spectrum of action.

Polyethylsiloxane fluid helps the skin absorb active substances.

Cyclomethicone - a tissue moisturizer.

Antifoam - a binder.

Lidocaine is an analgesic.

The optimal ratio of plant components in the mixture was revealed in the experiment on six types of wound healing agents based on concentrates from plant materials in the treatment of analogous rabbits by treating wounds. In rabbits, 300 ± 4 mm ² skin wounds were inflicted on the lateral side of the chest. The dynamics of changes in the area of wounds determined the course and time of healing. The results of the experiment are shown in table 1.

Table 1 Extract No. The content of plant components in the mixture in the production of extracts,% Duration of wound healing, days pharmaceutical camomile Birch buds Ornate common one one four 5 9,4 2 2 3 5 10.8 3 3 3 four 11.1 four 2 four four 10.8 5 one 5 four 10.5 6 3 2 5 11.8

As studies have shown, the most effective mixture in the treatment of wounds with extracts from a number of mixtures is extract No. 1 with a ratio of plant components - chamomile: buds: a fruit tree 1: 4: 5, the healing time was 9.4 days.

The optimal ratio of concentrates made from products of antler reindeer husbandry (concentrate from antlers of maaral) and medicinal plant materials (concentrate from chamomile, birch buds and common gooseberry) in the composition of the medicinal product was revealed in the experiment shown in Table 2. According to the combination of components, the wound healing treatment the product contained 1.0; 1.5; 2.0; 2.5% of each concentrate. Table 2 presents the average daily wound healing rate (%) and the duration of wound healing (days) according to the combination options.

The area, shape and place of wounding to rabbits - analogues were equivalent to the first experiment.

table 2 Indicator The average daily healing rate,% and the healing period of days when concentrates are contained in a wound healing medicine,% 1,0 and 1,0 1.5 and 1.5 2.0 and 2.0 2.5 and 2.5 The average daily rate of wound healing,% 12.69 14.58 14.61 14.63 Duration of wound healing, days 7.9 6.9 6.9 6.9

As can be seen from the research results shown in Table 2, an increase in the speed of wound healing and, accordingly, a reduction in the duration of wound healing occurred when concentrates were added to the composition of the wound healing agent in the amount of: 1.5% concentrate from antlers and 1.5% concentrate from plant materials. Given the need to save concentrates and the insignificant difference between the average daily wound healing rate when the drug is 1.5; 2.0 and 2.5% of concentrates, the optimal content of each concentrate in the composition of the product should be considered 1.5%. The shelf life of the wound healing agent was 1.8-2 years.

Pathological changes in the body and local complications objectively indicates leukocytosis. After wounding, the number of leukocytes in the blood was determined in the dynamics of the study (table 3). Comparison of the best sample (experiment) made on the basis of antler concentrate (1.5%) and concentrate from plant materials (1.5%) was performed with control 1 (wounds in rabbits were not treated) and control 2 (wounds in rabbits were treated with drug if it contains only 1.5% concentrate from plant materials).

Table 3 Period Control 1 without treatment of wounds Control 2 Experience 1.5% concentrate from plant materials 1.5% and 1.5% 0 days 6.3 * 10 ⁹ / L 6.38 * 10 ⁹ / L 6.40 * 10 ⁹ / L 3 days 10.19 * 10 ⁹ / L 7.91 * 10 ⁹ / L 7.21 * 10 ⁹ / L 5 days 9.98 * 10 ⁹ / L 7.22 * 10 ⁹ / L 6.54 * 10 ⁹ / L 7 days 8.75 * 10 ⁹ / L 6.71 * 10 ⁹ / L 6.34 * 10 ⁹ / L

In the second experiment, it was found that the use of agents, the composition of which included 1.5% concentrate from antlers and 1.5% concentrate from plant materials, caused a shift of the leukogram to the left and lymphopenia. The number of leukocytes in peripheral blood increased and was maximum on the 3rd day in all groups of animals. A significant decrease in the number of leukocytes was noted in the experimental group (1.5% and 1.5%) already on the 5th day, and by the 7th the level of leukocytes was normal.

The experience of using a wound healing drug is presented by example.

An example of the manufacture of a healing medicine. They take 500 g of pharmacy chamomile, 2 kg of birch buds, 2.5 kg of common fruit bush, mix well with 100 liters of distilled water (1:20) and extract at 57 ° C for 2.2 hours, after filtering the extract is dried at 40 ° C and a vacuum of 0.8 atm to a moisture content of 6%. 100 g of the concentrate obtained are mixed with 100 g of deer antler concentrate, add 33.3 g of stearic acid, 36.7 g of glycerol monostearate, 20 g of beeswax, 20 g of cosmetic wax, 66.7 g of lanolin, 400 g of vegetable oil, 200 g of glycerol, 20 g of sodium tetraborate, 40 g of triethanolamine, 20 g of phenochene, 33.3 g of polyethylsiloxane liquid, 100 g of cyclomethicone, 12.7 g of antifoam and 34.7 g of lidocaine with perfume and 4.8 liters of distilled water. The resulting product is well mixed and Packed in a sterile container.

Thus, the development of the claimed wound healing drug expands the arsenal of drugs, which allows more efficient use of valuable antler reindeer husbandry when using herbal medicinal properties, with a high wound healing effect of the product and a long shelf life.

Claims (2)

1. Wound healing drug, including a concentrate from antler deer raw materials, characterized in that the deer antler concentrate and a concentrate from a mixture of pharmacy chamomile, birch buds and common gooseberry taken in a ratio of 1: 4: 5, respectively, and extracted from subsequent drying in a ratio of a mixture of plant components: water 1:20, while the product additionally contains stearic acid, glycerol monostearate, beeswax, cosmetic wax, lanolin, vegetable oil, glyc Erin, sodium tetraborate, triethanolamine, phenochene, polyethylsiloxane liquid, cyclomethicone, antifoam, lidocaine + perfume, water in the following ratio, wt.%:
deer antler concentrate 1.5% concentrate from a mixture of vegetable components 1.5% stearic acid 5% glycerol monostearate 5.5% beeswax 0.3% cosmetic wax 0.3% lanolin 1,0% vegetable oil 6.0% glycerol 3.0% sodium tetraborate 0.3% triethanolamine 0.6% phenochen 0.3% polyethylsiloxane liquid 0.5% cyclomethicone 1.5% antifoam 0.19% lidocaine + perfume 0.52% distilled water rest 2. The tool according to claim 1, characterized in that the extraction of plant materials is carried out at a temperature of 55-60 ° C for 2.0-0.5 hours to an extract density of 5.0-5.5%, and drying is carried out at temperature not higher than 40 ° С and vacuum from 0.8 to 1.0 atm to a moisture content of 6.0-8.0%.