RU2490882C1 - Method of correction of embryonic and post-embryonic development of poultry - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture, in particular, to a method of correction of embryonic and post-embryonic development of poultry. The method of correcting the embryonic and post-embryonic development of poultry includes treatment of eggs. The treatment of eggs is carried out twice - before placing in the setter and when transferring the eggs for hatching, by method of irrigation by applying to the surface of eggs with the sprayer of 0.1% aqueous solution of zinc glycinate, and 2 days prior to vaccination, the birds are given zinc glycinate solution in a dose of 2.5 mg/kg body weight.

EFFECT: use of the claimed invention enables to adjust the natural resistance and to stimulate the metabolic processes in embryonic and post-embryonic periods of poultry development, as well as to increase the hatchability of eggs and hatching chickens.

12 tbl

Description

The invention relates to agriculture, in particular to poultry farming, specifically to methods of incubation of eggs and correction of zinc salt of glycinate with immunostimulating, immunomodulating properties that increase nonspecific reactivity of the body, hatchability of eggs, hatching of chickens, as well as productivity of poultry.

Known methods of using lithium glycinate [1, 2], glycine [3], zinc glycinate [4]. Methods [1, 2] (the use of lithium glycinate) we accept as a prototype.

The aim of the invention is the stimulation of embryogenesis, natural resistance, increasing the hatchability of eggs and hatching, correction of natural resistance and stimulation of metabolic processes with zinc glycinate.

Zinc is an element of the molecular structure of more than 80 known enzymes and is involved in the regulation of the activity of more than 200 enzyme systems, has an immunostimulating effect - zinc is involved in almost all parts of the immune system: in the process of maturation of lymphocytes and cellular immunity reactions, it increases the level of thymine and thymosin produced by the central an immunity organ - a thymus; increases the protective antimicrobial activity of neutrophils and macrophages; increases the production of protective antibodies. This microelement exhibits an antioxidant effect - zinc is the active center of one of the important enzymes - superoxide dismutase, which provide antioxidant protection of the body.

Glycine in the body acts as a inhibitory mediator and refers to metabolic regulators, due to which it has an antistress and nootropic effect, it is actively used in many anabolic processes that occur during bird embryogenesis.

The combination of zinc and the amino acid glycine in the form of a chelate compound, zinc glycinate, provides high bioavailability, digestibility and effectiveness compared to inorganic zinc salts, and zinc is absorbed most effectively (up to 60-70%) from aqueous solutions [5].

This goal is achieved by double treatment of the hatching eggs (before laying in the incubation cabinet and when transferring the eggs to the conclusion) with a 0.1% solution of zinc glycinate followed by treatment by evaporating zinc glycinate at a dose of 2.5 mg / kg live weight per head for 2 days prior to vaccination.

The proposed method for processing hatching eggs and poultry is economical because low concentrations of the preparation are required, zinc glycinate is highly active, non-toxic and used in medicine, in particular in therapy, to maintain the activity of enzymes associated with energy metabolism, bone mineralization and tissue synthesis; to maintain immunity, when taking drugs that block the absorption of zinc.

The proposed method is illustrated by the following examples (see Appendix).

Literature

1. Lukicheva V.A. The effect of the immunomodulator on protein-amino acid metabolism during vaccine stress of broiler chickens / B.A. Lukicheva, E.Yu. Penshina, A.V. Ermolaev, P.A. Ershov, K.M. Mironov, O.V. Zhen // Transactions of the XVII Moscow International Veterinary Congress. - S.249.

2. Lukicheva V.A. Adaptogenic effect of treatment of hatching eggs with lithium glycinate under oxidative stress caused by routine vaccination of chickens of the parent herd / B.A. Lukicheva // Agricultural Biology, 2009. - No. 4. - p. 67-70.

3. Bryushinin N.V. The effectiveness of the complex treatment of hatching eggs of meat chickens with solutions of succinate and glycine // N.V. Bryushinin / Mat. Int. scientific - practical conf. Dubrovitsy, 2003 .-- S.297-300.

4. Manukyan A.V. The use of biocomplexes of zinc and manganese in the cultivation of broiler chickens / A.V. Manukyan // Conf. and asp. on poultry farming: Abstracts. - Sergiev Posad, 2008 .-- S.19-20.

5. Khabarov A.A. Zinc: relevance and characteristics of dietary supplements (literature review) [Electronic resource] / A.A. Khabarov, E.V. Budko, K.A. Lushov and others // Modern problems of science and education, 2012. - No. 3. - Access mode: http://www.science-education.ru/103-6416 (accessed: 03.21.2013).

Table 1. Biological control indices during double treatment of hatching eggs in optimal concentration with zinc glycinate preparation Blood rings,% The consignment Neoplod,% Fade% Suffocating% Weak,% The output of chickens,% Egg hatchability,% The control 6.22 4.30 6.69 1.91 0.47 80.38 ± 1.90 85.71 ± 1.73 Zinc Glycinate 0.1% 5.20 4.49 6.38 - - 81.32 ± 1.69 88.39 ± 1.47

Table 2. The dynamics of the influence of zinc glycinate on the content of red blood cells in the blood of broiler chickens, 10 ⁹ / l (n = 10) age Groups 1 day 41 days The control 1.87 ± 0.09 1.82 ± 0.04 Zinc glycinate 2.5 mg / kg 1.93 ± 0.06 2.42 ± 0.08 *** Note: * -if P <0.05; ** - if P <0.01; *** - if P <0.001

Table 3. Dynamics of the influence of zinc glycinate on the content of leukocytes in the blood of broiler chickens, 10 ¹² / l (n = 10) age Groups 1 day 41 days The control 21.89 ± 0.13 31.43 ± 0.61 Zinc glycinate 2.5 mg / kg 22.63 ± 0.46 33.51 ± 0.77 Note: * -if P <0.05; ** - if P <0.01; *** - if P <0.001

Table 4. Dynamics of the influence of zinc glycinate hemoglobin content in the blood of broiler chickens, g / l (n = 10) age Groups 1 day 41 days The control 101.14 ± 1.10 117.35 ± 7.01 Zinc glycinate 2.5 mg / kg 107.53 ± 2.46 128.19 ± 3.72 ** Note: * -if P <0.05; ** - if P <0.01; *** - if P <0.001

Table 5. The dynamics of the effect of zinc glycinate on the hematocrit,% (n = 10) Groups age 1 day 41 days The control 23.3 ± 0.11 30.15 ± 0.09 Zinc glycinate 2.5 mg / kg 25.6 ± 0.16 * 37 ± 0.42 *** Note: * -if P <0.05; ** - if P <0.01; *** - if P <0.001

Table 6. The content of total protein in blood serum, g / l. (n = 10) Age Groups M ± m, g / l 1 day The control 50.41 ± 4.75 Zinc glycinate 2.5 mg / kg 53.98 ± 2.89 41 days The control 55.58 ± 3.78 Zinc glycinate 2.5 mg / kg 61.24 ± 3.46 *

Table 7. Dynamics of the influence of zinc glycinate on the production of protein substances, g / l. (n = 10) WHO-
rast groups Prealb Alb Postalb Tf Cp β ₂ Sa ₂ Y ₁ β-Lp The control 2.4 ± 0.23 18.43 ± 0.09 5.89 ± 0.24 10.7 ± 0.14 0.97 ± 0.11 3.62 ± 0.18 3.8 ± 0.11 2.78 ± 0.17 4.52 ± 0.13 1 day Zinc glycinate 2.5 mg / kg 2.6 ± 0.14 * 22.71 ± 0.12 ** 7.37 ± 0.19 *** 13.1 ± 0.21 ** 1.12 ± 0.18 * 4.89 ± 0.16 * 4.11 ± 0.08 ** 3.22 ± 0.14 ** 5.31 ± 0.33 * 41 days The control 1.84 ± 0.11 16.21 ± 0.46 3.99 ± 0.07 12.35 ± 0.14 1.80 ± 0.03 2,50 ± 0,15 3.28 ± 0.31 2.93 ± 0.12 4.22 ± 0.17 Zinc glycinate 2.5 mg / kg 2.34 ± 0.06 ** 18.21 ± 0.13 * 4.31 ± 0.06 *** 15.45 ± 0.11 *** 2.24 ± 0.02 ** 2.89 ± 0.22 *** 4.73 ± 0.13 *** 3.46 ± 0.16 *** 5.76 ± 0.09 *** Note: * - if P <0.05; ** - if P <0.001; *** - if P <0.0001

Table 8. The dynamics of the effect of zinc glycinate on ACT activity, mccat / l (n = 10) Groups / Metric Age 1 day 41 days The control 62.24 ± 3.15 64.94 ± 1.09 Zinc glycinate 2.5 mg / kg 63.78 ± 1.83 66.13 ± 3.27 Note: * - if P <0.05; ** - if P <0.01; *** if P <0.001

Table 9. The dynamics of the influence of zinc glycinate on ALT activity, mkkat / l (n = 10) Groups / Metric Age 1 day 41 days The control 4.85 ± 0.097 5.13 ± 0.09 Zinc glycinate 2.5 mg / kg 5.01 ± 0.07 5.41 ± 0.06 Note: * - if P <0.05; ** - if P <0.01; *** if P <0.001

Table 10. The bactericidal activity of blood serum of broiler chickens,% (n = 10) Group / Indicator Age 1 day 41 days The control 2.33 ± 0.06 22.69 ± 0.08 Zinc glycinate 2.5 mg / kg 3.99 ± 0.14 28.86 ± 0.31 * Note: * - if P <0.05; ** - if P <0.01; *** if P <0.001

Table 11. Lysozyme activity of blood serum of broiler chickens, μg / ml (n = 10) Age Group / Buyer 1 day 41 days The control 12.24 ± 0.03 14.19 ± 0.18 Zinc glycinate 2.5 mg / kg 14.31 ± 0.05 16.39 ± 0.02 * Note: * - if P <0.05; ** - if P <0.01; *** if P <0.001

Table 12. The effect of zinc glycinate on the productivity and safety of broiler chickens Indicator Group control zinc glycinate 0.1% The average live weight of 1 head, g 1790 2060 ** Slaughter yield,% 71.65 73.18 The mass of gutted carcass, g 1460 1790 The average daily gain, g 43.6 50,2 The cost of feed per 1 kg of growth, kg 1.91 1.9 Preservation,% 94.7 97.2 * Note: * -if P <0.05; ** - if P <0.01; *** - if P <0.001;

Claims (1)

A method for correcting embryonic and postembryonic development of poultry, including processing of eggs, characterized in that the processing of eggs is carried out twice (before laying in the incubation cabinet and when transferring the eggs to the conclusion) by irrigation by applying 0.1% aqueous spray to the surface of the eggs zinc glycinate solution, and 2 days before vaccination begins, birds are given a solution of zinc glycinate at a dose of 2.5 mg / kg body weight.