RU2706550C1 - Method for increasing resistance, meat and egg productivity of poultry head, in particular broiler chickens and laying hens in industrial poultry breeding - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture and poultry keeping. Disclosed is a method for increasing resistance, meat and egg productivity of poultry head, in particular broiler chickens and laying hens in commercial poultry industry, involving use of antioxidants in poultry rations, wherein antioxidant of polyphenol structure is used – sodium salt of [poly-(2,5-dihydroxyphenylene)]-4-thiosulphonic acid, which is introduced into feedstuff, mash feed or other types of fodder mixtures and/or products for birds at rate of 50–100 g/t product, adding 2.5–10 mg/kg of body weight to the bird drinking system individually, wherein the broiler chickens and the other poultry raised for meat – from the first day to the slaughtering, to the laying hens and the egg direction bird – for the first two months from the first day of life, and then during the whole period of productive use of livestock.

EFFECT: invention allows to expand the range of antioxidant agents used in poultry keeping, increase the daily meat poultry meat gain, accelerate the egg direction to the egg production peak and prolong productive use, increase of general organism resistance to negative effects of environment and diseases, survival rate of livestock, increase of productivity, improvement of quality and ecological safety of meat.

1 cl, 2 dwg, 14 tbl, 3 ex

Description

The present invention relates to veterinary medicine, agriculture and poultry farming, in particular, to the cultivation of broiler chickens and laying hens and obtaining their products and can be used in the preparation of feed, feed additives, mixtures, when it is necessary to increase the health and productivity of animals.

Currently, in the feeding of animals, ready-made (factory-made) feeds and additives are increasingly being used to maintain the vitality and health of the animal’s body both at home and in agriculture. For their manufacture, various biologically active substances are used that have such an effect on animals as: increasing the body's resistance and immune status, activating and normalizing metabolic processes, improving health indicators, increasing productivity and more.

However, not all known biologically active substances can be added to manufactured feeds, mixtures and additives for animals. This may be due to: various functional effects on different types of animals in view of the characteristics of metabolism, loss of functional qualities of a substance after heat treatment; due to changes in the state of aggregation; with a decrease in shelf life after adding to the product and other options.

It is known that the introduction of antioxidants and natural adaptogens into feeds ensures high preservation of young animals, increase in live weight, general resistance and productivity of birds, and reduces stress levels [6, 8, 12].

It is known that various groups of vitamins (vitamins A, E, C and others) are used in poultry farming as antioxidants, which increase the indicators of the general resistance of the animal organism, increase productivity and improve product quality [2, 15, 17].

It is known to use sulfur-containing compounds in poultry such as methionine, cysteine, sodium thiosulfate, etc. [3, 4] as antioxidants that increase the safety of the poultry stock and their qualitative and quantitative indicators of productivity.

It is known that succinic acid and preparations based on it, for example, the preparation emicidin (Mexidol), are widely used in agriculture and veterinary medicine. They are recommended in poultry farming for laying hens, broiler chickens as antioxidant, anti-stress drugs, to increase the safety of livestock and live weight of birds, egg production and more [7, 10, 13, 14, 18]. The effect of succinic acid and preparations based on it on metabolic processes has long been comprehensively studied [9]. So, in the work of M.N. Kondrashova [11] has shown that succinate activates energy metabolism in chickens. In addition, it was found that the use of succinic acid stimulates embryogenesis, increasing the viability of poultry embryos by activating metabolic processes, in particular protein [5], etc. Moreover, the use of emicidin in feeding broilers and laying hens gives the best results in comparison with the action of pure succinic acid in terms of qualitative and quantitative indicators of productivity, as well as the general health and safety of the livestock [14].

A known method of using succinic acid in chickens at a dose of 0.1 g / kg body weight before stress exposure, which improves the body's resistance to stress factors. Amplified LASK (lysozyme activity of blood serum), BASK (bactericidal activity of blood serum), the number of protein fractions increases, the safety of the livestock, as well as general and average daily gain. Succinic acid reduces the toxic effect on the body. In studies, it was found that the use of succinic acid also increases the level of antioxidant defense indices [1].

The disadvantage of this method is the insufficiently high indicators of productivity (quantity and quality) and nonspecific resistance of the organism of chickens.

The closest in technical essence and the achieved positive effect, and adopted by the authors as a prototype, is a method of using emicidin for broiler chickens - a double dose of 2.5 mg / head in poultry ration from a day old for 10 days and from 20 days in within 10 days, which positively affects the formation of muscle tissue structure, which tends to improve the technological properties of meat. Production testing proves the positive effect of emicidin, since the live weight of broiler chickens of the experimental groups increases by 2.4-6.7% compared with the control, the safety of the bird in the experimental groups is 1.2-1.5% higher [14].

The disadvantage of this method is the narrowness of the method of use as an antioxidant agent only with the aim of improving the productivity and preservation of the number of broiler chickens.

The task of the invention is to provide a method for increasing the resistance, meat and egg productivity of a poultry head, in particular broiler chickens and laying hens, which is highly effective and efficient, improves livestock health, increases the quantity and quality of products and is at the same time easy to use in industrial poultry farming .

The problem is solved by creating a method of increasing the resistance, meat and egg productivity of poultry heads, in particular broiler chickens and laying hens, in industrial poultry farming, including the use of a polyphenolic antioxidant in nature, sodium salt [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene, oliphene), which is introduced in the production of compound feed, a blender or other type of feed mixtures / products for birds at the rate of 50-100 g / tonne of product, is added to the watering system or fed to the bird individually 2.5-10 mg / kg of body weight - to broiler chickens and other poultry bred for meat - from the first day to slaughter, to laying hens and poultry eggs - the first two months from the first day of life, then throughout term of productive use of livestock.

It is important to consider that the result of feeding the substance will be noticeable for arbitrary periods (at least 7 days) and time periods of appointment. Also, the antioxidant can be given to the bird individually with drink and food.

The inventive method allows to expand the range used in poultry antioxidant agents.

Chemically, mitofen is the sodium salt of [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (NaS2 C6n O2n + 3 4n + 1) first entered the Russian market in 1997 in the form of the drug “olifen” [16].

The drug has the ability to activate cell metabolism, increases the resistance of animals to stress, normalizes the functioning of cellular systems in humans.

It consists of sequentially interconnected hydroquinone structures with a chain length of 2 to 6. The presence of a thiosulfuric acid fragment in the structure provides the drug with water solubility, and the phenolic groups of mitophene contribute to the effective functioning of free radical traps.

An important advantage of mitophene, unlike most other antioxidants, is its prolonged action. The molecule of most antioxidants is able to bind one or two free radicals, after which it turns into a ballast product, which must be removed from the body. It is very important that in the mitophene molecule, as it interacts with the radicals, the hydroquinoid fragments are oxidized and turn into quinones. The enzyme DT-diaphorase, present in the tissues, restores quinones to phenols, providing regeneration of the initial structure.

The drug does not have carcinogenicity and mutagenicity. The toxicity of the drug is low. It can be prepared in the form of traditional dosage forms, such as a solution in ampoules, tablets, ointments, capsules.

Feeding the diet with the sodium salt of [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene, oliphene) contributes to an increase in live weight of poultry meat, in particular broiler chickens due to an increase in muscle tissue, as well as faster organ development and body systems. The quality of meat is improved by increasing the amount of muscle mass, reducing the fat content in muscle tissue, reducing the layer of subcutaneous fat. The safety of the livestock increases due to the prevention of stressful conditions of various etiologies and an increase in the general non-specific resistance of the bird organism.

The invention allows to increase the average daily gain in live weight of poultry meat, in particular broiler chickens, the general resistance of the body to negative environmental influences and diseases, the survival rate of the livestock, as well as to increase productivity, improve the quality and environmental safety of meat.

Feeding the diet with the sodium salt of [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene, oliphene) contributes to an increase in the live weight of poultry eggs, in particular, faster development of organs and systems of the body, including maturation of the reproductive system, which has a positive effect on the production of eggs. There is an improvement in average indicators of egg quality (shell thickness, shell mass, yolk mass, protein mass, ratio of protein mass to yolk mass, etc.), improvement in average indicators of the condition of an intact egg (egg mass, egg volume, shape index, air diameter cameras, etc.). The safety of the livestock increases due to the prevention of stressful conditions of various etiologies and an increase in the general non-specific resistance of the bird organism.

The invention allows to speed up the output of poultry eggs, in particular, laying hens, to the peak of egg production and to extend the time of productive use, increases the overall resistance of the body to negative environmental influences and diseases, improves livestock survival.

Thus, the claimed technical result is achieved in the invention.

Analysis of the sources of information available to the authors allows us to conclude that the claimed method meets the criteria of NOVELTY, since the authors are not aware of the use of sodium (poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene, oliphene) as poultry antioxidants.

A comparative analysis of the claimed invention with known methods allows us to conclude that the claimed technical solution meets the criterion of "INVENTIVE LEVEL".

The claimed technical solution meets the criterion of "INDUSTRIAL APPLICABILITY", as it can be used in the cultivation of broilers and laying hens on breeding and commodity farms, as well as in private farms, which is also confirmed by specific examples.

Examples of specific performance.

Studies conducted by the Laboratory of Pharmacology and Toxicology of VNIVIP showed that polyphenolic antioxidants (in particular, mitophen) have pronounced antioxidant and antihypoxic properties. They have been tested in general medical practice in the treatment of ischemic conditions of various genesis, with obstructive bronchitis and other diseases accompanied by hypoxic phenomena. These properties of the sodium salt antioxidant [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene, oliphene) can be useful and in demand in industrial poultry farming.

The acute toxicity of the sodium salt [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene, oliphene) was studied in chicken embryos and chickens up to 40 days of age. It is noted that they have very low toxicity (4th grade). This allowed us to consider it promising for study and further use in poultry farming.

Work was carried out in which it was found that the sodium salt of [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene, oliphene) can be used in industrial poultry farming for chickens of egg and broiler directions in doses of 2.5-10 mg / kg of body weight as a highly effective antioxidant that increases productivity and improves product quality.

Example. 1 The experiment was conducted in vivarium VNIVIP on broiler chickens of the COBB 500 cross.

Since the main point of the antioxidant effect of the sodium salt of [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] on the body’s metabolism is energy metabolism, its use is reflected to some extent on the intensity of the Krebs cycle. At the same time, the components of this cycle themselves, the main of which are citrate, malate, succinate and fumarate, are also antioxidants that affect the energy metabolism of a living organism. In this regard, of course, it is relevant to compare the antioxidant and other effects of the sodium salt [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] with organic acids. For this purpose, mexidol (analogues: mexidol-vet, emicidin), a derivative of succinic acid, was chosen as the comparison drug, as the most prominent representative of this group of antioxidants.

The experiment was started on broilers of daily age. The preparations were given to the chickens from the second day inside (individually) in the form of a solution (1%) (30 days) with the corresponding course (table 1). As the main diet we used full feed, which meets all the requirements for the quality and nutrient content necessary for the correct and speedy development of poultry for each phase of chickens produced by the Gatchinsky Feed Mill.

The results of an experiment to study the effect of mitophen and mexidol on the intensity of the weight gain of chickens are presented in table 2.

* - P <0.05; ** - P <0,01 derived when comparing the indicators of experimental and control groups of birds.

In this experiment, with obvious reliability, the increase in the mass of chickens during the study period is ahead of the group of chickens treated with mitophene. This was reflected in feed conversion when feeding mitophen and mexidol, in particular, in chickens treated with mitofen (1.59) it was significantly better than control (2.16) and chickens given mexidol (1.78).

The study of the main hematological parameters of the chickens of the experimental and control groups did not reveal significant differences that allow us to make any reliable judgment on the issue of the influence of the tested substances. And, although some indicators of the leukocyte formula differ in different groups, it can be concluded that the use of mitophen or mexidol does not adversely affect the blood condition and general resistance indices of experimental chickens (table 3).

* - P <0.05; ** - P <0.01 derived when comparing the indicators of experimental and control groups of birds

At the same time, a biochemical study of the blood of chickens from experimental and control groups (Table 4) showed some differences that fit into the idea of the action of antioxidants. Thus, a significant decrease in the level of the hepatic enzyme alanine aminotransferase (ALT) was observed in the experimental bird of both experimental groups treated with antioxidants, which indicates the beneficial effect of the studied drugs on the detoxifying function of the liver. In addition, mitofen, in contrast to Mexidol, significantly reduced the level of creatinine and uric acid in the blood of experimental chickens. These indicators generally reflect the endotoxic load on the body and, in particular, indicate optimal kidney function.

* - P <0.05; ** - P <0,01 derived when comparing the indicators of the experimental and control groups of birds

In this experiment, we also performed a lysosomal-cationic blood smear test for broiler chickens after using mitofen and mexidol for a month. Despite the fact that no significant (significant) differences were found, there is a tendency for a higher level of lysosomal-cationic proteins in chickens treated with antioxidants (average 1.01 PIECES) in contrast to chickens in the control group (average 0.87 PIECES) .

Thus, the analysis of the obtained data suggests that in the studied dose range under the given experimental conditions, the use of the sodium salt [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene) shows greater efficiency in terms of increasing productivity, health and resistance , olifene) in comparison with mexidol.

Example. 2 The experiment was conducted in the conditions of vivarium VNIVIP on laying hens of the cross Highsex Brown.

The next most important question in the study of the pharmacological effect of mitophen on the resistance of chickens is the understanding of its effect on non-specific immunity during preventive measures, in particular, vaccination of birds against a number of dangerous infectious diseases. A similar interest in industrial poultry farming is relevant for many infectious diseases.

To study this issue, experience was set up to determine the effect of feeding mitophene on the health and indicators of nonspecific immunity of egg chickens starting from the day old when conducting preventive measures, in particular, vaccination against metapneumovirus infection and Newcastle disease.

The experiment was started on egg chickens from 1 day old and was carried out for 60 days with the appropriate course (table 5). As the main diet we used full feed, which meets all the requirements for the quality and nutrient content necessary for the correct and speedy development of poultry for each phase of chickens produced by the Gatchinsky Feed Mill.

The delivery of chickens was carried out from the Roskar poultry farm. Day-old chickens are vaccinated against Marek’s disease, infectious bronchitis and infectious bursal disease (Gumboro disease). After 5 days, chickens of the I-III groups were vaccinated against metapneumovirus infection and Newcastle disease with an inactivated vaccine (oil) subcutaneously in the region. the middle third of the neck at a dose of 0.3 ml. The term of use of mitophen is 2 months.

Data on the dynamics of the increase in the mass of chickens of the egg direction with and without mitofen during immunization with MPVI and NB are shown in table 6.

* - P <0.05; ** - P <0.01; *** - P <0.001 derived when comparing the parameters of experimental and control groups of birds

For chickens of egg direction, the intensity of weight gain is not a paramount indicator, of course, provided they are well preserved and healthy. Nevertheless, regular monitoring of the gain of growing young animals is important for understanding the correctness of its physiological development. Analysis of this table reveals certain differences in the intensity of weight gain in vaccinated chickens fed with mitophene, in contrast to chickens in the control group. It is of certain interest that these differences are statistically significant only during the first month of life. In the future, the rate of weight gain in chickens of the egg direction is leveled, however, according to the average weight gain, the trends continue.

In our opinion, it is important that by the end of the study period:

- the average weight of all vaccinated chickens is slightly higher than that of the control ones, which indirectly can serve as evidence of the need and safety of vaccination.

- the average weight of the bird receiving mitofen is higher than that of a simply vaccinated and control bird.

- the average weight of the bird treated with the antioxidant, the higher, the greater the dose of mitophene.

Thus, the feasibility of using mitophene at the initial stage of raising chickens, especially during vaccinations, is confirmed.

It is known that most drugs are neutralized (destroyed) mainly in the liver. Therefore, the time out of anesthesia is a kind of indicator of health, body resistance, which allows you to simulate the detoxification ability of the liver. Therefore, as part of this experiment, we conducted a test with anesthesia of the bird using propofol, the result of which is shown in Table 7, Figure 1. Propofol was injected intravenously into the axillary vein at a dose of 8 mg / kg live chicken weight. This dose caused anesthetic sleep. The chicks' exit time from anesthetic sleep was recorded by raising the head, opening the eyes, and reaction to external stimuli.

* - P <0.05 derived from a comparison of experimental and control groups of birds

For clarity, a figure is presented (Fig. 1), which reflects these data in percent (for 100% the time taken for the chickens to exit the anesthesia of the control group).

An analysis of the data obtained allows us to conclude that the appointment of mitophene in the tested doses helps to increase the detoxification function of the liver during immunization with MPVI and NB pathogens. The best detoxification indicators were identified with the use of 50 g / ton of feed, which manifests itself in a faster exit of the bird from propofol anesthesia. This suggests the possibility of using the test substance for prophylactic and therapeutic purposes in cases of intoxication with poisons that damage the liver, in particular, mycotoxins.

It was found that the appointment of mitofen in doses of 25-50 g / ton of feed contributes to a significant increase in the level of hemoglobin and leukocytes in the blood of chickens (table 8) during vaccination against MPVI and NB. This makes it possible to suggest a positive effect of mitophen in the pathology of the hematopoietic system (for example, anemia of chickens of various origins) as well as in pathology accompanied by a decrease in immunity (viral pathology).

* - P <0.05; ** - P <0.01 derived when comparing the indicators of experimental and control groups of birds

Analysis of the leukocyte formula suggests that both vaccination of chickens against MPVI and NB, and the combined use of an antioxidant with the corresponding vaccines do not adversely affect the white blood of experimental chickens (table 9). There may be some interest in the tendency to increase the number of stab pseudo-eosinophils in vaccinated chickens, which indirectly indicates the immunomodulating effect of vaccination. It was noted that this trend is more pronounced in chickens fed mitofen with food. This phenomenon is consistent with the data of the lysosomal-cationic test (table 11 and Fig. 2), which statistically significantly shows an increase in the level of lysosomal-cationic proteins in the blood granulocytes of chickens fed with the antioxidant.

* - P <0.05 derived from a comparison of experimental and control groups of birds

A separate question also raises a noticeable decrease in the level of eosinophils relative to control in chickens receiving a large dose of mitophene. Given that the content of these cells reflects the level of sensitization of the body to foreign proteins (allergens), it can be assumed that the antioxidant used in certain doses may manifest a desensitizing effect. However, this requires a separate study.

A study of the content of malondialdehyde (table 10) in the blood of chickens showed that the administration of mitophene to the chickens with food helps increase the antioxidant activity of the blood (which directly correlates positively with the dose). This shows the beneficial effect of mitophene on the antioxidant system of the bird.

* - P <0.05; ** - P <0.01 are derived when comparing the indicators of experimental (I, II) and control groups of birds

* - P <0.05 was derived when comparing the indicators of experimental and control groups of birds

It was found that the use of mitophen in a dose of 25-50 g / ton of feed contributes to a significant increase in the level of lysosomal cationic proteins in chicken blood cells. This gives reason to argue about the positive effect of mitophene on the nonspecific resistance of the body of egg chickens.

Summing up the results of this experiment, we can confidently state that during preventive measures, in particular, vaccination of birds against metapneumovirus infection and Newcastle disease, oral administration of mitophen during the period of intensive growth of egg chickens (from one day old to two months old) leads to increase of nonspecific resistance of the body of chickens, favorably affects the antioxidant status of the body and helps to enhance the detoxifying function of the liver (based on and propofol test). In addition, a favorable effect of the proposed mitofen course on some blood hematological parameters was noted. In particular, a tendency, under the influence of an antioxidant, to an increase in the content of hemoglobin and leukocytes within physiologically permissible limits, which indicates an increase in the defenses of the body of chickens, is noted.

Example. 3 The experiment was carried out in the conditions of vivarium VNIVIP on laying hens of the cross Highsex Brown.

An important parameter in industrial poultry farming of the egg direction is the quality and preservation of eggs, including during long-term storage. The aim of this experiment was to study the effect of laying hens of salt [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene, oliphene) with feed and feed additive containing succinic acid on some physical parameters of the egg.

In the experiment, laying hens of 120 days old cross-country Highsex brown vaccinated at a poultry farm according to generally accepted standards were used. It was formed 4 groups of 10 goals each, 3 experimental and control. From the beginning of the experiment, mitophen at the rate of 50 g per ton of feed was given to laying hens of the experimental group along with the main diet. From the beginning of the experiment, laying hens of experimental group II of the experimental group were given mitofen at the rate of 50 g per ton of feed and a feed additive containing 10% succinic acid in an amount of 1.5 kg per ton of feed. From the beginning of the experiment, only laying feed containing 10% succinic acid in the same amount was given to laying hens of the experimental group III, along with the main diet. As a feed additive containing 10% succinic acid, the composition was used "Energy", manufactured by LLC "PTK" PeterBio ". Three months after the start of the experiment, an egg was taken from the bird to determine the quality immediately and after 5 months of storage, 30 pcs. The study of a number of physical parameters of egg quality was carried out by generally accepted methods.

Results and discussion. The quality parameters of the eggs, determined before the selection of eggs for storage are shown in table 12.

* - P <0.05; ** - P <0.01; *** - P <0.001 derived when comparing the parameters of experimental and control groups of birds

Thus, the use of the sodium salt of [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene, oliphene) for 3 months as a feed additive to the diet at a dose of 50 mg / kg of feed (experimental group I) to laying hens from 4 months of age, contributes to a change in a number of quality indicators of the eggs obtained relative to the control, including, respectively:

1. Thickening of the shell.

2. An increase in the number of pores in the shell by 29.5%, the yolk index - by 9.3%, the ratio of the mass of protein to the mass of yolk - by 17.4% and the relative weight of the protein - by 6.2%.

3. A decrease in protein pH by an average of 0.3.

By analyzing the results of the experimental group II, one can trace the tendency of the synergistic effect of mitophene with a feed additive on egg quality indicators, in comparison with all other groups:

At the same time, with a significant increase in egg mass and volume, the remaining indicators are at a high level, as in the I and III experimental groups in relation to the control.

In addition, the use of mitofen in laying hens in the indicated doses does not lead to a significant change in the mass, volume of the egg, the relative mass of the shell, the density of the egg and the pH of the yolk.

Thus, the totality of the data obtained in this experiment indicates the absence of negative effects and the positive effect of the antioxidants used on egg quality.

After five months of storage in a household refrigerator at a temperature of + 3 ° ± 1 °, a change in egg mass was noted, which is reflected in table 13.

Analysis of table 13 shows that the completely natural process of egg mass loss is not significantly different in all groups. However, it is necessary to take into account that in the experimental groups 27-30.5% more pores in the shell were noted than in the control. If the quality of the eggs obtained from the control and experimental birds were absolutely identical, one would expect that the weight loss of the eggs of the experimental group would be much higher. Therefore, in this case, one can assume better preservation and higher quality of eggs from chickens treated with mitophen antioxidant and feed additive, both together and separately.

In addition to insignificant loss of egg weight during storage, no other damage, inferiority or technical defect were observed in the selected batches.

The egg quality parameters determined after five months of storage are shown in table 14

* - P <0.05; ** - P <0.01; *** - P <0.001 and derived when comparing the indicators of experimental and control groups of birds

Egg quality indicators from laying hens treated with mitofen are slightly different from the control. In general, the same positive trends remain as with the initial egg laying. However, according to a number of signs, significant changes occurred during storage:

1. The height of the air chamber in the egg from the experimental hens increased significantly less than in the control group, which indicates the best preservation of the egg from the hens of the experimental groups.

2. During storage, the difference in the index of the protein index between the groups significantly increased. So, if in eggs from experimental chickens of group I this indicator decreased on average from 5.96 to 5.67, which, given the scatter, can be considered statistically insignificant. At the same time, the egg from the hens of the control group reduced this indicator from 5.88 ± 0.22 to 4.59 ± 0.24 over 5 months of storage, which is very significant and indicates a deterioration in the protein quality of the eggs being studied. The protein index of the eggs of the experimental group II decreased from 5.62 ± 0.26 to 4.76 ± 0.20, which allows us to talk about the trend of better egg preservation by this indicator in relation to control.

Thus, within the framework of this experiment, it can be argued that the use of the sodium salt of [poly (2,5-dihydrooxyphenylene) -4-thiosulfonic acid] (mitophene, oliphene) in the diet of laying hens contributes to the improvement of egg quality and its safety. In addition, the synergistic effect was revealed with the simultaneous use of antioxidants of different classes (in particular, organic acids and polyphenols) in the diet in therapeutic doses.

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18. Fisinin, V.I. Poultry farming in Russia - innovation development strategy / V.I. Fisinin - M. - 2009 .-- 148 p.

Claims (1)

A method of increasing the resistance, meat and egg productivity of a poultry head, in particular broiler chickens and laying hens in industrial poultry farming, including the use of antioxidants in the diet of a bird, characterized in that they use a polyphenolic structure antioxidant - sodium salt [poly- (2,5-dihydroxyphenylene) )] - 4-thiosulfonic acid, which is introduced into the feed, mixes or other types of feed mixtures and / or products for birds at the rate of 50-100 g / t of product, is added to the watering system or 2.5-10 mg / kg are individually fed to the bird mass t la, and broiler chickens and other birds reared for meat - on the first day before slaughter, laying hens and poultry egg direction - the first two months from the first day of life, and then for the duration of the productive use of livestock.

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