RU2520092C1 - Method of stimulating embryogenesis of laying hens - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: method comprises a single treatment of hatching eggs before laying into the incubator from sprayer with the aerosol of 0.01% sodium salt solution of lipoic acid, which is preliminary dissolved and mixed in distilled water at a temperature of 18-22°C. The solution is applied to the surface of egg shells 3-4 hours prior to their incubation.

EFFECT: method enables to reduce lipid peroxidation processes, to optimise the metabolic rate, to increase the intensity of hatching at high viability of young birds for a long period of post-embryonic development.

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Description

This invention relates to agriculture, in particular to poultry, and can be used to prevent the effects of technological stress in chicken crosses during critical periods of development of the chicken embryo.

It is known that the effect of technological stresses leads to the activation of free radical processes [4] and increases lipid peroxidation in the tissues of the chicken embryo, which causes significant economic damage to the poultry industry [1, 2]. Therefore, it is important to prevent these phenomena using environmentally friendly natural metabolites [3, 5]. The use of sodium salt of lipoic acid for the treatment of hatching eggs is unknown. Lipoic acid and its salts are actively used in medicine, but its use in agriculture is extremely small. Sodium salt of lipoic acid is involved in the oxidative decarboxylation of pyruvic acid and a - ketoacids, in the process of energy formation in the body, in the regulation of lipid and carbohydrate metabolism, has a lipotropic effect, affects cholesterol metabolism, improves liver function [6]. The use of the sodium salt of lipoic acid is due to its good solubility in water, while lipoic acid itself is insoluble in water. It is worth noting that this metabolite has never been used to prevent technological stresses in poultry farming, and its effect on the chicken embryo is not known.

The purpose of the invention is the prevention of technological stress in chicken crosses during critical periods of development of the chicken embryo by reducing lipoperoxidation processes, optimizing the level of metabolism.

The technical result is the stimulation of embryonic development of egg chickens, an increase in the output of conditioned chickens, the prevention of technological stresses.

This result is achieved by reducing lipid peroxidation processes and optimizing the level of metabolism in critical periods of development of the chicken embryo, by treating the hatching eggs once, namely, before laying the aerosol from the spray bottle on the egg shell, apply a 0.01% solution of lipoic acid sodium salt.

The method is as follows:

An aqueous solution of lipoic acid sodium salt in a concentration of 0.01% is applied to the surface of the shell 3-4 hours before incubation from the spray gun. Preliminarily, the preparation is dissolved and mixed in distilled water at a temperature of 18-22 ° C.

Example 1. Eggs of hens of the cross "Shaver Brown" were obtained from one parent herd, sorted by 306 pieces in each batch according to the principle of analogues, taking into account the time of demolition and shelf life and weight. A solution of the preparation was applied on the surface of the shell as an aerosol according to the above scheme. As a control group, eggs of the same batch were selected according to the principle of analogues, which were processed according to the technology adopted at the farm. The data obtained are presented in table 1. It can be seen from the table that the withdrawal from the experimental batch exceeds the control by 11.77%, and the output is 11.03%. Such incubation wastes significantly decreased as: “frozen” by 4 times, “suffocated” by 3 times, and “weak” by 5.1 times.

Example 2. The laying of eggs of the cross "Shaver Brown" for incubation of the experimental and control groups was made 05.03.2012 at 12 hours. 03/25/2012, the withdrawal began, lasting 29 hours, which is 9 hours ahead of the withdrawal rate. So, the development of allantois in the experimental group on the 11th day is ahead of control, as can be seen from table 2.

table 2 Categories of eggs by the degree of allantois closure,% n = 100 The consignment
Category
eggs by
allantoisu 1st category 2nd category 3 category The control 20,0 36.0 44.0 Experience 23.0 47.0 30,0

Example 3. Chickens hatched from processed eggs (example 1) were grown under the conditions of the same farm up to 30 days of age in cell batteries. The data are presented in table 3. In the experimental group over the entire study period, the safety of young animals increased by 1%, respectively, compared with the control.

Table 3 The safety of chickens,%. n = 100 Groups of chickens Control Experienced Age in days 1-10 case four 3 save 96 ± 1.96 97 ± 1,71 10-20 case one one save 99 ± 0.99 99 ± 0.99 20-30 case one one save 99 ± 0.99 99 ± 0.99 In just 30 days case 6 5 save 94 ± 2,37 95 ± 2.18

Example 4. In chickens hatched from processed eggs, at the age of 24 hours, a biochemical study of blood serum was established: the total protein content in the blood serum of the chickens of the experimental groups increased by 5.3%, α-amylase by 8.2%, and blood glucose 0.9%, an increase in lysozyme activity by 4.6%. The data are presented in table 4.

Table 4 Biochemical blood parameters. n = 5 Group Indicator Control Experienced Total protein, g / l 31.9 ± 1.34 33.6 ± 1.21 Albumin, g / dl 4.13 ± 0.32 4.25 ± 0.35 ** Urinary acid, umol / l 0.30 ± 0.007 0.34 ± 0.006 * α-amylase, u / l 1067 ± 91.3 1155 ± 94.2 *** Glucose, mmol / l 9.24 ± 0.08 9.33 ± 0.07 ** Total lipids, g / l 1.52 ± 0.018 1.63 ± 0.009 ** Ca, mmol / l 3.2 ± 0.05 3.22 ± 0.08 P, mmol / l 2.06 ± 0.075 1.94 ± 0.081 ** Alkaline phosphatase, U / l 1202 ± 14.21 1260 ± 13.53 *** Lysozyme, mkg / ml 35.0 ± 0.30 36.6 ± 0.25 **

As can be seen from table 5, the drug has an antioxidant effect in the body of chickens. The table shows that the intensity of peroxidation in the experimental group is lower than in the control. Thus, the level of the secondary product of lipid peroxidation in the form of malondialdehyde (MDA) decreased by 12.5%. Also, with a decrease in the intensity of lipid peroxidation processes, the antioxidant enzymatic protection of the organism of day old chickens increased, which resulted in an increase in superoxide dismutase (SOD) by 1.5 times. All biochemical parameters were within the physiological norm.

Table 5 Indicators of lipid peroxidation (lipid peroxidation) and antioxidant defense system (AODS). Group peroxidase, unit opt.pl./l * s SOD, act / mg of hemoglobin OSh, rel.ed / ml MDA, μmol / L Control 23 ± 2.12 1.0 ± 0.12 0.35 ± 0.013 1.6 ± 0.16 Experience 25 ± 1.78 ** 1.5 ± 0.16 *** 0.3 ± 0.017 ** 1.4 ± 0.19 ***

Thus, based on the conducted experiments, there is an increase in the intensity of withdrawal with high viability of young animals for a long period of postembryonic development. In this case, the hatching of chickens in the experimental batch was higher by 11.77% (p <0.001), and the hatchability of eggs by 11.03% (p <0.001) compared with the control group.

Literature

1. Ageechkin A.P. Industrial poultry farming / A.P. Ageechkin, F.F. Alekseev, A.V. Aralov et al. // Ed. IN AND. Fisinina, Sergiev Posad .: GNU VNITIP of the Russian Agricultural Academy. - 2010 .-- 599 s.

2. Baraboy V.A. Peroxidation and stress / V.A. Baraboy, I.I. Brekhman, Yu.B. Golotin, Yu.B. Kudryashov // St. Petersburg. - The science. - 1992. - 148 p.

3. Burlakov S.S. The effect of biologically safe methods of processing eggs on the embryonic development of chickens / S.S. Burlakov // All-Russian. conf. young scientists and graduate students in poultry: Abstracts dokl. - Sergiev-Posad. - 1999. - 11 p.

4. Vladimirov Yu.A. Free radicals in living systems / Yu.A. Vladimirov, O.A. Azizova, A.I. Deev, A.V. Kozlov, A.N. Osipov, D.I. Roshchupkin // Results of Science and Technology. - Biophysics series. - vol. 29. - Moscow. - VINITI. - 1992 .-- 250 s.

5. Ermolova Yu.S. Processing eggs of hens with biologically active drugs to stimulate resistance of chickens at various stages of ontogenesis: abstract of thesis. Cand. biol. sciences. / Ermolova Yu.S. // M. - 2010 .-- P.23.

6. Peace Joseph. Alpha-lipoic acid - a universal remedy against free radicals, cell aging / Joseph Peace // M .: Dilya. - 2009 .-- 96 s.

Claims (1)

A method of stimulating embryogenesis of egg chickens during critical periods of chicken embryo development by reducing lipid peroxidation processes, optimizing metabolic rate, increasing the intensity of hatching with high viability of young animals for a long period of postembryonic development, including a single treatment of hatching eggs, in particular, before spraying an aerosol from the spray bottle into the incubator 3-4 hours before their incubation, a 0.01% solution of lipoic acid sodium salt, which dvaritelno mixed and dissolved in distilled water at a temperature of 18-22 ° C.