RU2486751C1 - Method of physiological acceleration of embryos of chickens - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to poultry breeding, in particular, to a method of physiological acceleration of embryos of chickens. The method of physiological acceleration of embryos of chickens, reduction of free-radical oxidation, optimisation of metabolic process with high viability of poults during a long period of post-embryonic development comprises treatment of hatching eggs. The treatment of hatching eggs is carried out on a single occasion 3-4 hours before placing in the incubator, while an aqueous solution of the preparations is applied with a sprayer on the surface of the egg shell: ribav, succinic acid and colamine at concentrations of 0.5%, 0.1%, 0.1%, respectively, and the preparations are preliminary dissolved in distilled water at 18-22°C.

EFFECT: use of the invention enables to accelerate the embryogenesis of egg chickens, to obtain standard youngsters with a high degree of antioxidant protection, the optimal level of metabolic processes and high immunity level.

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Description

This invention relates to the field of agriculture and, in particular, to poultry farming (aerosol application of the solution, which includes ribav, colamine, succinic acid).

Known treatment of hatching eggs with solutions of ribaves, colamine and succinic acid in order to stimulate the development of agricultural birds. Ribav has a wide range of pharmacological actions: immunostimulating, anti-inflammatory, hepatoprotective and wound healing activity, antitoxic and growth-promoting effect. It helps to increase the natural resistance of animals, stimulates erythropoiesis, metabolism, improves gastrointestinal motility, succinic acid and its salts have adaptogenic ability and have antihypoxic, anti-stress, antioxidant and neurotropic effects, normalize energy and plastic metabolism and the general physiological state of the body. Succinic acid enhances biochemical and physiological recovery processes in pathological conditions. Colamine inhibits the oxidation of fats, vitamin A and other compounds with unsaturated carbon bonds, takes an active part in redox processes in animals, improves phosphorus and protein metabolism and, therefore, is a growth stimulator. The combined use of these drugs is not jointly registered. Also, these natural metabolites have never been used to fully accelerate chick embryogenesis.

The effectiveness of these methods, taken by us as prototypes, lies in the fact that ribav and succinic acid were used in aerosol treatment of hatching chicken eggs. Egg hatchability increased by 5-7.01%. With a single pre-incubation treatment of meat chicken eggs with colamine solutions, egg hatchability was 3.5–4.24% (p <0.05) higher.

The purpose of the invention is a significant acceleration of embryogenesis of egg chickens, obtaining conditioned young animals with a high degree of antioxidant protection, an optimal level of metabolic processes, high immunity, by a single aerosol treatment of hatching eggs with a solution of succinic acid, colamine and ribaves.

The method is as follows - on the surface of the shell of eggs of chickens 3-4 hours before incubation from the spray gun, apply an aqueous solution of drugs: ribav, succinic acid and colamine in concentrations of 0.5%, 0.1%, 0.1%, respectively. Preliminarily, the preparations are dissolved and mixed in distilled water at 18-22 ° C.

Example 1. Eggs of hens of the cross "Shaver White" were obtained from one parent herd, sorted by 306 pieces per batch, subject to egg similarity, time of laying, and shelf life. On the surface of the shell, a solution of the preparations was applied in the form of an aerosol according to the above scheme. As a control, eggs of the same batch were selected according to the principle of analogues, which were processed using farm technology. The data obtained are presented in table 1. From the table it follows that the conclusion from the experimental batch exceeds the control by 10.46%, and the deduction by 9.26%. Such incubation wastes significantly decreased: “blood rings” 2.24 times, “frozen” 1.5 times, “suffocated” 2.4 times, “weak” 4.5 times.

Example 2. The laying of eggs of the cross "Shaver White" for incubation of the experimental and control lots was made 04.07.11, at 12.00. On the morning of July 24, 2011, at 5 p.m., the hatching of the experimental group ended, which is 1.5 days ahead of the set incubation time (day 21). The chickens of the experimental group already in the embryonic period exceeded the control in developmental intensity. So the development of allantois in the experimental group on the 11th day is significantly ahead of control, as can be seen from table 2.

The energy of the hatching intensity is shown in Figure 1. From which it is seen that this indicator in the experimental batch was higher and by the time the control group started to hatch, all the experimental young animals had already hatched from the eggs. The drawing of the output intensity also clearly demonstrates the fact that the chickens in the experimental group almost immediately after hatching go to the stage of mass withdrawal (peak occurs at 446 hours), while mass withdrawal in the control requires a significantly longer amount of time (peak falls at 490 hours).

Example 3. Chickens hatched from processed eggs (example 1) were grown under the conditions of the same farm up to 60 days of age in cell batteries. The data are presented in table 3. In the experimental group over the entire study period, the safety of young animals increased by 3%, respectively, compared with the control.

Table 3 The safety of chickens,%, n = 100 Groups of chickens Control Experienced Age in days 1-10 case 2 save 98 ± 1.4 one hundred 10-20 case one 2 save 99 ± 0.99 98 ± 1.4 20-30 case 2 one save 98 ± 1.4 99 ± 0.99 30-40 case - - save one hundred one hundred 40-50 case - - save one hundred one hundred 50-60 case one - save 99 ± 0.99 one hundred In just 60 days case 6 3 save 94 ± 2,37 97 ± 1,71

Example 4. In chickens hatched from processed eggs, at the age of 24 days, a biochemical study of blood and blood serum was found: these biologically active substances solutions, when used in combination, helped to optimize some metabolic processes, in particular protein (the total protein content in the blood serum of experimental group chickens increased 8.29-21.68%). In addition, there is a pattern of activation of carbohydrate metabolism, which resulted in an increase in α-amylase activity by 1.2-1.7 times, and blood glucose in 1.5-1.9 times. In connection with an increase in PVC levels by 1.6 times, LDH by 1.3 times, one can judge the activation of both anaerobic and predominant aerobic glycolysis. The content of pentoses in 1.4 times more also led to a higher relationship between exchanges - carbohydrate, protein and metabolism of nucleic acids. Obviously, this relationship can to some extent “support” the body in the future under stressful situations, making it possible for the compensatory functions of each of the exchanges to be necessary, which is necessary to increase the adaptive capabilities of the body.

It is also obvious that colamine from the proposed mixture is also effectively incorporated into phospholipid complexes, which was expressed primarily in an increase in lecithins by 1.3 times. Therefore, with any “planned” and “not planned” stressful influences, the chicken body “requests” components of phospholipids for elimination of structural membrane destruction. And the colamine from our mixture to some extent covers these needs.

All this influenced the increase in the natural resistance of the organism of chickens, which was also evidenced by an increase in lysozyme activity by 4.18-10.31%.

Thus, this level of metabolic processes in chickens of the experimental group and its intensity, as well as the observed patterns are optimal, based on the fact that the experimental individuals were better developed and highly viable over a long period of life, with a shorter embryonic development. The data are presented in table 4.

Table 4.1 Indicators of lipid peroxidation (LPO) and antioxidant defense system (AOSZ) Group peroxidase unit opt.pl./l * s SOD, act / mg of hemoglobin OSh, rel.ed / ml MDA, μmol / L Control 24 ± 2,00 1.0 ± 0.15 0.4 ± 0.018 1.7 ± 0.181 Experienced 50 ± 3.74 *** 2.8 ± 0.36 0.2 ± 0.025 1.1 ± 0.114

As can be seen from table 4.1, the drug has a powerful antioxidant effect in the body of chickens. The table shows that the intensity of peroxidation in the experimental group is significantly lower than in the control. So, the level of the secondary product of lipid peroxidation in the form of malondialdehyde (MDA) decreased 1.5 times, and the final product, Schiff base (OS), 2 times. An interesting fact is that, with a decrease in the intensity of peroxidation processes, the antioxidant enzymatic protection of the body of chickens of day old increased, which resulted in an increase in superoxide dismutase (SOD) of 2.8 times. All biochemical parameters are within the physiological norm.

Literature

1. Azarnova T.O. Pre-incubation treatment of Haysex White cross-country eggs with the ribav drug and its effect on chicken embryogenesis / T.O. Azarnova // Poultry farm. - M., 2005. - No. 12. - 35-36 s.

2. Bryushinin N.V. The effect of various doses of succinate and glycine on protein and energy metabolism in the process of growth and development of chickens / N.V. Bryushinin // All-Russian. scientific method, conf. on pet hygiene, init. Danilova A.K .: Thesis. Dokl - M., 2003 .-- 89-93 p.

3. Azarnova T.O. The effectiveness of certain biologically active substances (ribav, succinic acid, glycine) on the formation of natural resistance of egg chickens / T.O. Azarnova, M.S. Naidensky, S.Yu. Zaitsev // Poultry farm. - M., 2006. - No. 5. - 37-38 p.

4. Kostandi O.X. Increasing the resistance of egg cross chickens by processing hatching eggs with organic acids: // Dis. ... cand. vet. Sciences: / O.K. Kostandi; MGAVMiB. - M., 2000. - 15 p.

5. Naumenko L.I. Physiological condition and productive qualities of chickens and ducks when consuming Ribav-1: Abstract. dis. ... cand. s / s. Sciences: / L.I. Naumenko; Belgorod state. Agricultural Academy. - Belgorod, 1999. - P.20.

6. Topuria L.Yu. Ribav phytopreparation - an effective immunostimulant for poultry / L.Yu. Topuria // The first international conference dedicated to the 300th anniversary of St. Petersburg: Abstract. doc. - M., 2003 .-- S.144-147.

Claims (1)

The method of physiological acceleration of chicken embryos, reduction of free radical oxidation, optimization of the metabolic process with high viability of chickens for a long period of postembryonic development by processing incubation eggs, characterized in that the processing of incubation eggs is carried out once 3-4 hours before laying in the incubator, while the spray shell surface of the eggs is sprayed with an aqueous solution of drugs: ribav, succinic acid and colamine in concentrations of 0.5%, 0.1%, 0.1%, respectively, and Paratov previously dissolved in distilled water at 18-22 ° C.