RU2422041C1 - Additive to drinking water for poultry and its application method - Google Patents

Abstract

FIELD: food industry. ^ SUBSTANCE: invention relates to agriculture, in particular, to poultry industry, namely, to additives to drinking water increasing poultry performance. The additive to drinking water for poultry contains streptomycin sulphate in an isotonic solution of sodium chloride at a weight ratio of 1:1000. The additive is once given to poultry to drink at an age of 26-36 days for 4-14 days until slaughtering. The additive is introduced into water in an amount of 0.0085-0.0095 ml per 1 kg of poultry live weight. ^ EFFECT: invention allows to produce a highly efficient and environmentally safe additive to drinking water ensuring poultry performance increasing due to increase of live weight gain at a low cost of the additive production and application. ^ 2 cl, 3 tbl, 3 ex

Description

The invention relates to agriculture, in particular to poultry, and in particular to additives to drinking water that increase the productivity of chickens.

It is known to use dihydroethoxyquinine as an additive to the feed of broiler chickens (see RF patent No. 2364246, class IPC A23K 1/00, publ. 10.08.2009), which is an antioxidant of ethoxyquin (Santokhin). The additive is introduced in an amount of from 20 to 200 mg per kilogram of dry matter of the main feed. The drug can also be introduced into drinking water. The introduction of the additive provides an increase in the productivity of broiler chickens by 1.2-3.8%.

However, the supplement is a biologically active substance, the use of which can lead to irreversible consequences not only in the body of the bird, but also in a person who consumes such products in food.

It is also known to use when feeding chickens an aqueous suspension of ultradispersed basalt (see RF patent No. 2280982 according to class IPC A01K 67/02, published on 08/10/2006). This suspension allows you to mainly increase the immunity of birds and their egg production.

However, the suspension does not significantly increase the productivity of birds. In addition, the substance used (ultradispersed basalt) is a volcanic rock, the main scope of which is construction. The use of this substance requires special permission from Rospotrebnadzor and a sanitary-epidemiological report.

Closest to the claimed is an additive to drinking water, increasing the productivity of birds, and the method of its application. The additive contains at least two microorganisms selected from the group consisting of Enterococcus, Lactobacillus, Pediococcus and Bifidobacterium (RF patent No. 2372788 according to IPC A23K 1/00, published on November 20, 2009). In particular, it may consist of 1 × 10 ¹⁰ -1 × 10 ¹³ CFU / kg of drinking water supplement Enterococcus faecium, DSM 16211 and 1 × 10 ¹⁰ -1 × 10 ¹³ CFU / kg of drinking water supplement Lactobacillus reuteri, DSM 16350 .

However, the additive is complex in composition, requires lengthy preparation, high labor costs and, as a result, has a high cost.

The invention is aimed at solving the problem of creating a highly effective and environmentally friendly additive to drinking water, providing increased productivity of chickens by increasing live weight gain at low cost for the manufacture of the additive and its use.

To solve the problem, an additive to drinking water for chickens containing the active part contains, according to the invention, as the active part of streptomycin sulfate and additionally contains a solvent in the form of an isotonic sodium chloride solution with a ratio of weight parts of streptomycin sulfate to an isotonic sodium chloride solution of 1: 1000 .

The method of application of the additive consists in a single watering out of a bird aged 26-36 days 4-14 days before slaughter, and the additive is added to water at the rate of 0.0085-0.0095 ml per 1 kg of live weight.

Known to the authors of the sources of patent and scientific and technical information, additives for drinking water for chickens and the method of its application, which consists in using streptomycin sulfate as an additive in an isotonic sodium chloride solution in weight ratios of 1: 1000, are not described.

The use of streptomycin sulfate in an isotonic sodium chloride solution is known (see, for example, Instructions for the use of the drug Streptomycin; sites http://dic.academic.ru; www.xumuk.ru/farmacevt; and others), while streptomycin powder ( a bottle of 0.5-1 g or 500000-1000000 DB) is diluted in 2-7 ml of isotonic sodium chloride solution. Apply streptomycin sulfate intramuscularly, as well as in the form of aerosols, intratracheally.

In known concentrations of streptomycin, sulfate is usually used to treat tuberculosis, as well as for purulent-inflammatory processes of various localization caused by gram-positive and gram-negative microorganisms: with pneumonia caused by Klebsiella, with plague and tularemia, brucellosis and endocarditis.

The authors first revealed a new property of streptomycin sulfate in dilutions, 1000 times smaller than during normal use, to affect the increase in the average daily gain of the body of chickens at the age of 26-36 days when they are fed with the claimed supplement. It was also revealed that watering should be carried out 4-14 days before the slaughter of the bird.

Moreover, on the basis of biochemical studies, it was proved that only in these concentrations, streptomycin sulfate solution acts to increase the live weight of the bird. The mechanism of action of the drug is based on a change in the ratio of aerobic and anaerobic oxidation processes in the direction of strengthening the anaerobic process.

The foregoing allows us to conclude that there is a “inventive step” in the claimed solution.

Streptomycin is an antibiotic that is formed during the life of radiant fungi Streptomyces globisporus streptomycini or other related microorganisms. Organic base: N-methyl-a-L-glucosamido-b-2-streptosidostreptidine. Available in the form of sulfate.

Chemical name: O-2-Deoxy-2- (methylamino) -alpha-L-glucopyranosyl (1-2) -O-5-deoxy-3-C-formyl-alpha-L-lixofuranosyl (1-4) -N , N, bis (aminoiminomethyl) -D-streptamine (as sulfate).

Streptomycin sulfate - powder or porous mass of white or almost white, odorless, bitter taste; hygroscopic. Easily soluble in water, practically insoluble in alcohol, chloroform and ether. Streptomycin sulfate is stable in a slightly acidic environment, but is easily destroyed in solutions of strong acids and alkalis when heated.

Doses are calculated in terms of weight or in units of action (DB): 1 unit is equal to 1 μg of chemically pure streptomycin base.

Sodium chloride - cubic crystals or white crystalline powder, salty taste, odorless. Soluble in water (1: 3).

An aqueous solution of sodium chloride for injection is a colorless, clear, salty taste liquid. The solution is sterile, pyrogen-free.

Sodium chloride isotonic injection is used as a detoxification agent, to correct the condition during dehydration, to dissolve other drugs; as a hypertonic solution - for pulmonary, gastric and intestinal bleeding as an auxiliary osmotic diuretic to ensure forced diuresis; in conditions leading to a deficiency of sodium ions, chlorine, poisoning with silver nitrate, for the treatment of purulent wounds (local).

Additive to drinking water is prepared as follows.

Take 500-1000 ml (0.5 L - 1 L) of isotonic sodium chloride solution for injection at a concentration of 0.9%, add to it 0.5 g - 1 g (500000-1000000 UNITS) of streptomycin sulfate to obtain the finished product with content in 1 ml of sodium chloride 1000 PIECES of streptomycin.

The additive is added to drinking water at a dose of 0.85-0.95 ml per 1 liter of water.

Broiler chickens are fed at 26-36 days of age 4-14 days before their slaughter, taking into account existing norms of water consumption by birds of different age groups at the rate of 0.0085-0.0095 ml of additive per kg of live weight (8.5-9 , 5 PIECES of streptomycin sulfate per kg of live weight).

Example 1

Evidence of an increase in live weight of chickens after drinking them with water, based on small concentrations of streptomycin sulfate.

On the basis of six workshops of the poultry farm, six groups of broiler chickens were formed according to the analogue method: 3 experimental and 3 control.

In all experimental workshops, chickens at 28 days of age were once given drinking water with an additive, while:

- in the first experimental workshop at the rate of 0.7 ml of additive per 1 liter of water, i.e. with streptomycin sulfate content of 7 PIECES / kg of poultry live weight;

- in the second experimental workshop at the rate of 0.9 ml of additive per 1 liter of water, i.e. with streptomycin sulfate content of 9 PIECES / kg of poultry live weight;

- in the third experimental workshop at the rate of 1.5 ml of additive per 1 liter of water, i.e. with the content of streptomycin sulfate 15 U / kg of live weight of the bird.

Live weight was taken into account in birds at 28 days of age and 38 days of age, i.e. 10 days before slaughter.

The results of changes in live weight of the bird are presented in table 1.

From table 1 it follows that the most effective increase in live weight of the bird (by 14%) occurs after the birds are fed with water with the addition of 0.009 ml per kg of live weight (9 units of streptomycin sulfate).

When a bird is fed with water with an addition of less than 0.009 ml (in an amount of 0.007 ml per kg of live weight) or more (in an amount of 0.015 ml per kg of live weight), a decrease in the percentage of increase in live weight in comparison with the control is recorded by 0.5% and 5, respectively. 5%.

Thus, this experiment confirms the provision of effective poultry weight gain by introducing an additive to drinking water, having a ratio of streptomycin sulfate components to an isotonic sodium chloride solution of 1: 1000 and, as a result, introduced in an amount of 0.009 ml per 1 kg of live weight.

Example 2. The rationale for the slaughter of poultry after evaporating them with water with an additive.

The birds were selected at the age of 26 days, of which two groups were formed by the method of analogues: experimental and control in the amount of 5 animals each.

The birds were drunk with water with the addition of 0.009 ml per kg of live weight of the bird (9 IU of streptomycin sulfate). The live weight was recorded 4, 7, 11, 14 and 18 days after evaporation. The results are presented in table 2.

From table 2 it follows that after 4-14 days after feeding birds with water with the addition, the difference in the percentage of growth between the experimental and control values ranges from 6.8% to 8.2%.

With the introduction of the drug more than 14 days before slaughter, the weight gain in birds decreases and becomes lower than the control. In addition, the 18-day period after administration of the additive corresponded to the 44-day age of the bird, in which broiler meat has reduced organoleptic properties.

Example 3. Conducting biochemical studies to justify the increase in live weight after watering chickens with an additive to drinking water at a dose of 0.009 ml per kg of live weight of the bird.

The studies were carried out in groups 3 (control) and 4 (experimental), indicated in example 1, where in the experimental group the most effective gains in birds were manifested.

Blood sampling for biochemical analysis was carried out during the slaughter of the bird. Blood was taken from 20 experimental chickens and 20 control group chickens.

The values of aspartate aminotransferase, alanine aminotransferase, creatine kinase, alkaline phosphatase, lipase, glucose, urea, total protein, globulin, total cholesterol, as well as the ratio of total protein to urea and atherogenicity index were determined from biochemical parameters.

The results of biochemical analysis were subjected to statistical processing with the calculation of student criteria.

The results of biochemical studies are presented in table 3.

It was found that the activity of aspartate aminotransferase, alanine aminotransferase, creatine kinase, alkaline phosphatase and lipase, as well as the concentration of glucose and urea in experimental chickens, significantly decreased compared to the control.

The concentration of total protein, globulin, total cholesterol, as well as the ratio of total protein to urea and the atherogenic index in the experiment, on the contrary, increased (p <0.05).

Table 3 The results of biochemical studies of poultry blood after drinking them with an additive to drinking water at a dose of 0.009 ml per 1 kg of live weight of the bird groups
parameter M ± m M ± m REDUCED INDICATORS COMPARED TO CONTROL Aspartate aminotransferase 241.76 11,4 222.84 16,0 Alanine aminotransferase 69.76 4,5 39.41 3.9 Creatine kinase 6145.46 617.7 5519.54 555.2 Alkaline phosphatase 574.08 174.9 481.94 151.1 Lipase 5.14 0.4 4,58 0.6 Glucose 10.40 0.8 9.65 0.6 Urea 0.86 0.1 0.72 0.2 INCREASE OF INDICATORS COMPARED TO CONTROL Protein 47.58 2.6 59.35 3.4 Globulin 21.62 2,4 26.56 3.6 OHS 2.88 0.1 3.16 0.3 Total Protein / Urea 61.67 6.2 82.12 22.5 Atherogenic index 2.22 0.3 2.40 0.4

The results obtained indicate that the metabolism in chickens of the experimental group is more focused on the synthesis of organic substances than in the control group, and is characterized by the predominance of the anabolic metabolism. This conclusion is confirmed by higher in the experiment than in control, the values of the ratio of total protein and urea, reflecting the relationship of the catabolic and anabolic metabolism.

A decrease in lipase activity indicates a decrease in the breakdown of triglycerides and, accordingly, a decrease in the intensity of catabolic processes.

Based on the results of the study, biochemical indicators were selected that are the most informative in studying the dynamics of gain in broiler chickens. Thus, an increase in live weight (increase in weight gain) is accompanied by a decrease in the activity of creatine kinase, lipase, alkaline phosphatase, an increase in the concentration of total protein, cholesterol, the ratio of total protein and urea, and also the atherogenic index.

Thus, the increase in weight gain is due to the predominance of the anaerobic oxidation process over aerobic.

Similar studies were carried out for other ratios of the components of the additive, in particular when the ratio of streptomycin sulfate to isotonic sodium chloride solution 1: 100 and 1: 10000. At these ratios, the predominance of anaerobic processes over aerobic ones was not observed in the bird organism, and, as a result, there were no gain.

The inventive additive is effective, environmentally friendly, easy to manufacture, does not require special equipment for its use.

Claims (2)

1. Additive to drinking water for chickens containing an active part, characterized in that it contains streptomycin sulfate as an active part and additionally contains a solvent in the form of an isotonic sodium chloride solution with a ratio of weight parts of streptomycin sulfate to an isotonic sodium chloride solution of 1: 1000. 2. The method of applying the additive to drinking water for chickens, which consists in feeding the hens with water with the additive, characterized in that the drinking of the hens is carried out once at the age of 26-36 days 4-14 days before slaughter, and the additive according to claim 1 is added to the water at the rate of 0.0085-0.0095 ml per 1 kg of live weight.