WO2011090401A1

WO2011090401A1 - Drinking water additive for chickens and method for administering same

Info

Publication number: WO2011090401A1
Application number: PCT/RU2010/000772
Authority: WO
Inventors: Владислав Николаевич ЛАСКАВЫЙ; Сергей Иванович ИВАНЕНКО; Владимир Иванович ФИСИНИН; Валерий Николаевич CЕРГЕЕВ; Виктор Михайлович CТРЕМОУСОВ; Владимир Иванович ПАНФЁРОВ; Михаил Леонидович MAЛИНИН; Сергей Трофимович КОСТЕНКО
Original assignee: Laskavy Vladislav Nikolaevich; Ivanenko Sergei Ivanovich; Fisinin Vladimir Ivanovich; Sergeev Valeriy Nikolaevich; Stremousov Victor Mikhajlovich; Panfjerov Vladimir Ivanovich; Malinin Mikhail Leonidovich; Kostenko Sergei Trofimovich
Priority date: 2010-01-25
Filing date: 2010-12-21
Publication date: 2011-07-28
Also published as: RU2422041C1

Abstract

The present invention pertains to the field of agriculture and particularly relates to poultry breeding and to drinking water additives for enhancing the productivity of chickens. The purpose of the invention is to provide a high-efficiency, ecologically safe drinking water additive that enhances the productivity of chickens by increasing body weight gain, while reducing the costs of preparing and administering said additive. The drinking water additive for chickens contains streptomycin sulfate in isotonic sodium chloride solution in a weight ratio of 1:1000. The method for administering said additive comprises giving the same only once to chickens aged 26-36 days 4-14 days before slaughter, the additive being added to water in a proportion of 0.0085-0.0095 ml per kg of body weight.

Description

ADDITIVE TO DRINKING WATER FOR CHICKENS AND

METHOD OF ITS APPLICATION

The invention relates to agriculture, in particular to poultry, and in particular to additives to drinking water that increase the productivity of chickens.

It is known to use dihydroethoxyquinine as an additive to broiler chickens feed (see RF patent N ° 2364246, class IPC A23L1 / 00, publ. 10.08.2009), which is an antioxidant of ethoxyquin (Santokhin). The additive is introduced in an amount of from 20 to 200 mg per kilogram of dry matter of the main feed. The drug can also be introduced into drinking water. The introduction of the additive provides an increase in the productivity of broiler chickens by 1.2-3.8%.

However, the supplement is a biologically active substance, the use of which can lead to irreversible consequences not only in the body of the bird, but also in a person who consumes such products in food.

It is also known to use an aqueous suspension of ultradispersed basalt when feeding chickens (see RF patent 2280982, class IPC AO 1K 67/02, publ. 08/10/2006). This suspension allows you to mainly increase the immunity of birds and their egg production.

However, the suspension does not significantly increase the productivity of birds. In addition, the substance used (ultradispersed basalt) is a volcanic rock, the main scope of which is construction. The use of this substance requires special permission from Rospotrebnadzor and a sanitary-epidemiological report. Closest to the claimed is an additive to drinking water, increasing the productivity of birds and the method of its use. The additive contains at least two microorganisms selected from the group consisting of Enter ococcus, Lactobacillus, Pediococcus and Bifidobacterium (RF patent JY <> 2372788, class MGJ A23K1 / 00, publ. 20.1 1.2009). In particular, it can consist of 1x10 ¹⁰ ⁿ -1h10 CFU / kg drinking water additive Enterococcus faecium, DSM 16211 and ¹⁰ -1h10 1x10 ¹³ CFU / kg drinking water additive Lactobacillus reuteri, DSM 16350.

However, the additive is complex in composition, requires lengthy preparation, high labor costs and, as a result, has a high cost.

The invention is aimed at solving the problem of creating a highly effective and environmentally friendly additive to drinking water, which provides increased productivity of chickens by increasing live weight gain at low cost for the manufacture of the additive and its use.

To solve the problem, an additive to drinking water for chickens containing the active part contains, according to the invention, streptomycin sulfate as an active part and additionally contains a solvent in the form of an isotonic sodium chloride solution at a ratio of weight parts of streptomycin sulfate to an isotonic sodium chloride solution of 1: 1000 .

The method of application of the additive consists in a single evaporation of a bird aged 26-36 days 4-14 days before slaughter, and the additive is added to water at the rate of 0.0085-0.0095 ml per 1 kg of live weight.

The sources of patent and scientific and technical information known to the authors do not describe additives for drinking water for chickens and the method of its use, which are used as additives streptomycin sulfate in an isotonic sodium chloride solution in a weight ratio of 1: 1 LLC.

The use of streptomycin sulfate in an isotonic sodium chloride solution is known (see, for example, Instructions for the use of the drug Streptomycin; sites http://dic.academic.ru; www.xumuk.ru/farmacevt; and others), while streptomycin powder ( a bottle of 0.5-1 g or 500000-1000000 units) is diluted in 2-7 ml of isotonic sodium chloride solution. Apply streptomycin sulfate intramuscularly, as well as in the form of aerosols, intratracheally.

In known concentrations of streptomycin, sulfate is usually used to treat tuberculosis, as well as for purulent-inflammatory processes of various localization caused by gram-positive and gram-negative microorganisms: with pneumonia caused by Klebsiella, with plague and tularemia, brucellosis and endocarditis.

The authors first revealed a new property of streptomycin sulfate in dilutions, 1000 times smaller than during normal use, to affect the increase in the average daily gain of the body of chickens at the age of 26-36 days when they are fed with the claimed supplement. It was also revealed that watering should be carried out 4-14 days before the slaughter of the bird.

At the same time, on the basis of biochemical studies, it was proved that only in these concentrations a solution of streptomycin sulfate acts to increase the live weight of the bird. The mechanism of action of the drug is based on a change in the ratio of aerobic and anaerobic oxidation processes in the direction of strengthening the anaerobic process.

The foregoing allows us to conclude that there is a “inventive step” in the claimed solution. Streptomycin is an antibiotic that is formed during the life of radiant fungi Streptomyces globisporus streptomycini or other related microorganisms. Organic base: I-methyl-a-b-glucosamido-b-2-streptosidostreptidine. Available in the form of sulfate.

Chemical name: 0-2-Deoxy-2- (methylamino) -alpha-L-glucopyranosyl (1-2) -0-5-deoxy-3-C-formyl-alpha-b-lixofuranosyl (1 -4) -, , bis (aminoiminomethyl) -B-streptamine (as sulfate). Streptomycin sulfate - powder or porous mass of white or almost white, odorless, bitter taste; hygroscopic. Easily soluble in water, practically insoluble in alcohol, chloroform and ether. Streptomycin sulfate is stable in a slightly acidic environment, but is easily destroyed in solutions of strong acids and alkalis when heated.

Doses are calculated in terms of weight or in units of action.

(UNIT): 1 UNIT equals 1 μg of chemically pure streptomycin base.

Sodium chloride - cubic crystals or white crystalline powder, salty taste, odorless. Soluble in water (1: 3).

An aqueous solution of sodium chloride for injection is a colorless, clear, salty taste liquid. The solution is sterile, pyrogen-free.

Sodium chloride isotonic solution for injection - used as a detoxification agent, to correct the condition during dehydration, to dissolve other drugs; as a hypertonic solution - with pulmonary, gastric and intestinal bleeding as an auxiliary osmotic diuretic to ensure forced diuresis; in conditions leading to a deficiency of sodium ions, chlorine, poisoning with silver nitrate, for the treatment of purulent wounds (local). Additive to drinking water is prepared as follows.

Take 500 - 1000 ml (0.5 l - 1 l) of isotonic sodium chloride solution for injection at a concentration of 0.9%, add to it 0.5 g - 1 g (500,000 - 1,000,000 IU) of streptomycin sulfate to obtain the finished product with content in 1 ml of sodium chloride 1000 PIECES of streptomycin.

The additive is added to drinking water at a dose of 0.85-0.95 ml per 1 liter of water.

Broiler chickens are fed at 26-36 days of age 4-14 days before their slaughter, taking into account the existing norms of water consumption by birds of different age groups from the calculation of 0.0085-0.0095 ml of additive per kg of live weight (8.5-9 , 5 PIECES of streptomycin sulfate per kg of live weight).

Example 1

Evidence of an increase in live weight of chickens after drinking them with water, based on small concentrations of streptomycin sulfate.

On the basis of six workshops of the poultry farm, six groups of broiler chickens were formed according to the analogue method: 3 experimental and 3 control.

In all experimental workshops, chickens at 28 days of age were once given drinking water with an additive, while:

- in the first experimental workshop at the rate of 0.7 ml of additive per 1 liter of water, i.e. with streptomycin sulfate content of 7 PIECES / kg of poultry live weight;

- in the second experimental workshop at the rate of 0.9 ml of additive per 1 liter of water, i.e. with streptomycin sulfate content of 9 PIECES / kg of poultry live weight;

- in the third experimental workshop at the rate of 1.5 ml of additive per 1 liter of water, i.e. with the content of streptomycin sulfate 15 U / kg of live weight of the bird.

Live weight was taken into account in birds at 28 days of age and 38 days of age, i.e. 10 days before slaughter.

The results of changes in live weight of the bird are presented in the table

one.

From table 1 it follows that the most effective increase in live weight of the bird (by 14%) occurs after water is fed to birds with d _[ g _ru pyomer _r additive in the amount of 0.009 ml per kg of live weight (9 PIECES of streptomycin sulfate).

O O O K K K _[opytayat olntayanoa _p _p _p pytayaont pynenvieontolnolaimn

Prnayanayappyaya g _py n - - - and watering poultry water with the addition of less than 0.009 ml (in an amount of ₂₂ _2304980138 ₂₃₆₂₄ K ₂ ₂₄₆₆₂ _2574913 0olov _p ppevo g g _y Olich v, 007 ml per kg body weight) or more (0.015 ml per kg of live weight) a decrease in the percentage of live growth is recorded

b ₁ K ₀₀₀₉₁₅ ₀₀ ₀₀₀₇ aka Kgo Dovi nolichep _{g v} .. _,

mass compared ttslo _minutes and _ss s piy m ww _E, the control, respectively, 0.5% and 5.5%.

Table 1

Change in poultry live weight with the introduction of various amounts of drinking water additives

Live weight Live weight

birds to birds in live weight gain

28-day 38-day

age age

before administration after administration

additives, kg additives, kg

35 i

Total Single Total Single Total Single%

head head kg head kg kg

27977 1.123 41953 1, 684 13976 0.561 50.0

one

27079 1, 098 40495 1, 642 13416 0.544 49.5

2

24296 1.030 40333 1, 710 16037 0.68 66.0

3 -

23,624 1,000 42,523 1,800 18,899 0.80 80.0

four

20 360 0.886 36331 1.581 15971 0.695 78.4

5

20898 0.907 36128

6 1, 568 15 230 0.661 72.9 Thus, this experiment confirms the provision of effective poultry gains when introducing an additive to drinking water having a ratio of streptomycin sulfate components to an isotonic sodium chloride solution of 1: 1000 and, as a result, introduced in an amount of 0.009 ml per 1 kg of live weight.

Example 2

Substantiation of terms of slaughter of a bird after evaporation with water with an additive.

The birds were selected at the age of 26 days, of which two groups were formed by the method of analogues: experimental and control in the amount of 5 animals each.

The birds were drunk with water with the addition of at the rate of 0.009 ml per kg of live weight of the bird (9 IU of streptomycin sulfate). The live weight was recorded after 4, 7, 1 1, 14 and 18 days after evaporation. The results are presented in table 2.

Table 2.

Change in the gain in live weight of poultry at the age of 26 days depending on the timing of slaughter of the bird after drinking with water with the addition of 9 units of streptomycin sulfate per kg of live weight

Konayont zl _]

686.7 733.3 6.8% 816.6 18.9% 893.3 30, 1% 973.3 41, 7% 1 106.7 61%

From table 2 it follows that 4-14 days after the birds were fed with water with an additive, the difference in the percentage of growth between the experimental and control values is from 6.8% to 8.2%.

With the introduction of the drug more than 14 days before slaughter, the weight gain in birds decreases and becomes lower than the control. In addition, the 18-day period after administration of the additive corresponded to the 44-day age of the bird, in which broiler meat has reduced organoleptic properties.

Example 3. Conducting biochemical studies to justify the increase in live weight after watering chickens with an additive to drinking water at a dose of 0.009 ml per kg of live weight of the bird.

The studies were carried out in groups 3 (control) and 4 (experimental), indicated in example 1, where in the experimental group the most effective gains in birds were manifested.

Blood sampling for biochemical analysis was carried out during the slaughter of the bird. Blood was taken from 20 experimental chickens and 20 control group chickens.

The values of aspartate aminotransferase, alanine aminotransferase, creatine kinase, alkaline phosphatase, lipase, glucose, urea, total protein, globulin, total cholesterol, as well as the ratio of total protein to urea and atherogenicity index were determined from biochemical parameters.

The results of biochemical analysis were subjected to statistical processing with the calculation of student criteria. The results of biochemical studies are presented in table 3.

It was found that the activity of aspartate aminotransferase, alanine aminotransferase, creatine kinase, alkaline phosphatase and lipase, as well as the concentration of glucose and urea in experimental chickens, significantly decreased compared to the control.

The concentration of total protein, globulin, total cholesterol, as well as the ratio of total protein to urea and the atherogenic index in the experiment, on the contrary, increased (p <0.05).

Table 3.

The results of biochemical studies of poultry blood after evaporation

The results obtained indicate that the metabolism in chickens of the experimental group is more focused on the synthesis of organic substances than in the control group, and is characterized by the predominance of the anabolic metabolism. This conclusion is confirmed by higher values in the experiment than in the control, values the ratio of total protein and urea, reflecting the relationship of the catabolic and anabolic metabolic links.

A decrease in lipase activity indicates a decrease in the breakdown of triglycerides and, accordingly, a decrease in the intensity of catabolic processes.

Based on the results of the study, biochemical indicators were selected that are the most informative in studying the dynamics of gain in broiler chickens. Thus, an increase in live weight (increase in weight gain) is accompanied by a decrease in the activity of creatine kinase, lipase, alkaline phosphatase, an increase in the concentration of total protein, cholesterol, the ratio of total protein and urea, and also the atherogenic index.

Thus, the increase in weight gain is due to the predominance of the anaerobic oxidation process over aerobic.

Similar studies were carried out for other ratios of the components of the additive, in particular, when the ratio of streptomycin sulfate to isotonic sodium chloride solution 1: 100 and 1: 10000. At these ratios, anaerobic processes did not predominate over aerobic ones and, as a result, there were no weight gain .

The inventive additive is effective, environmentally friendly, easy to manufacture, does not require special equipment for its use.

Claims

CLAIM

1. Additive to drinking water for chickens containing an active part, characterized in that it contains streptomycin sulfate as the active part 5 and additionally contains a solvent in the form of an isotonic sodium chloride solution with a ratio of weight parts of streptomycin sulfate to an isotonic sodium chloride solution of 1: 1000 2. The method of applying the additive to drinking water for chickens, which consists in drinking chicken with water with an additive, characterized in that the chicken is drunk once at the age of 26-36 days 4-14 days before slaughter, and in water u make the additive according to claim 1 from the calculation of 0.0085-0.0095 ml per 1 kg of live weight.

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