RU2402232C2 - Dietary fodder supplement for broiler chickens - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to zootechny, veterinary science, agriproducts processing and may be used in fodder production. The broiler chicken dietary fodder supplement includes bifidus bacteria of the Bifidobacterium bifidum group in the form of "Bifidumbacterin dry lyophilisate", lactic acid bacilli Lb. Acidophilus in the form of dry Biobakton dietary supplement (DS), a dry dietary supplement of leafy mass of stevia and grass meal of clover. The components are taken in the following ratio, wt %: "Bifidumbacterin dry lyophilisate" -70; DS dry Biobakton - 10; dietary fodder supplement of leafy mass of stevia -10; grass meal of clover - 10. One adds the dietary fodder supplement to basal feed in an amount of 1%.

EFFECT: invention implementation allows to normalise microbiocenosis, improve digestibility of fodders due to activation of digestive tract activity, to improve protein-and-carbohydrate metabolism which will lead to liveweight gain increase and livability improvement.

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Description

FIELD OF THE INVENTION

The invention relates to livestock farming, veterinary medicine, crop production, processing of agricultural products and is intended for use in feeding broiler chickens and creating methods for maintaining the livestock of poultry by introducing probiotic and herbal additives into the diet for their activation to increase the enzymatic, increase bifidogenic and acidogenic activity of the gastrointestinal intestinal tract, regulation of protein-carbohydrate metabolism at various stages of feeding, acceleration of the adaptation period and improving the ecological state of the poultry organism and the environment.

BACKGROUND

A known method of obtaining a biologically active feed additive, which uses a combination of live cultures of probiotic bacteria Runninococcus, Lb. Acidophilus in a ratio of wt.%: Uterine culture of bacteria - 15-35, adsorbent - 65-85 (RF Patent No. 2190332, Cl. A23K 1/16, 2002), as well as probiotic feed additives containing animal bifidobacteria and streptococci ( Development of the Research Institute of Agriculture of the North-East, Kirov Region) - streptobifid, lacticide - live cultures of lactic acid bacteria and streptococci grown on milk whey (Development of State Research Institute of Applied Mathematics and Microbiology).

Known data on the stevia plant (Stevia rebaudiana Bertoni). Biochemical studies of stevia leaves showed that they have a low calorie content and the daily intake of dry stevia leaves does not exceed 10 g or 1 g of substances of the steviazide complex (A. A. Krivenko. Prospects for the use of a new sugar-bearing culture (Stevia rebaudiana Bertoni) / A.A .Krivenko, V.I.Zhabina, O.V. Mironova, A.I. Gavrilov, L.D. Ostroukhova //. III international practical conference "The state of the biosphere and human health" - Penza, 2003, pp. 91-93 .

Known feed additive from the leafy stem mass of stevia TU 9759-004-00668620-2007.

Known dietary supplement in the form of dietary supplement Biobactone dry, containing lactobacillus Lb. Acidophilus, which have antagonistic activity against a wide range of pathogenic and conditionally pathogenic intestinal microorganisms and create favorable conditions for the development of microflora and functions of the digestive tract and metabolic processes.

Representative of beneficial microflora are also bifidobacteria of the Bifidobacterium bifidum group. They have a bifidogenic effect and provide activation of the immune system, the formation of non-specific immune bodies, lower the pH of the intestinal contents, and create unfavorable conditions for the development of pathogenic microflora. Bifidobacteria of the Bifidobacterium bifidum group are presented in the form of the immunobiological preparation "Bifidumbacterin dry lyophilisate" FSP 42-0092-2847-02, certificate of conformity No. 000452, OKP code 01897209, manufactured by the Federal State Unitary Enterprise "Microgen" of the Ministry of Health of the Russian Federation.

It is known that protein plant components — seeded legume grass — red clover, blue alfalfa, sainfoin (V.I. Trukhachev, N.Z. Zlydnev, A.I. Podkolzin. Feeding farm animals in the North Caucasus: a monograph. - Ed. 3rd, revised and additional - Stavropol: Agrus, 2006. - 296 p.).

The closest in technical essence and the achieved positive effect and accepted by the authors for the prototype is Broiler Chicken Feed, application No. 20061053 57/13, containing up to 40% of grain of oats and enzyme preparations as sources of carbohydrates, essential amino acids and energy.

The disadvantage of this method is that its preparation uses expensive enzyme preparations.

The present invention, in comparison with the prototype and other known technical solutions, has the following advantages: the use of probiotic additives containing acido- and bifidobacteria, a dry vegetable feed additive from leafy stevia, grass flour from clover allows you to create a balanced system that activates the reflex and enzymatic functions of the gastrointestinal intestinal tract, protein-carbohydrate metabolism and providing the normobiocenosis of the animal organism, which leads to an increase in production GOVERNMENTAL factors: safety, average daily gain and reduced feed costs paid.

SUMMARY OF THE INVENTION

The objective of the invention is the development of a biologically active feed additive for broiler chickens, which could normalize the activity of the gastrointestinal tract, enhance enzymatic activity and protein-carbohydrate metabolism.

The purpose of the invention is the creation of a biologically active feed additive for broiler chickens using local probiotics and biologically active plant components for the treatment and prevention of non-communicable diseases, the normalization of microbiocenosis, the activation of the enzymatic activity of the gastrointestinal tract, the improvement of protein-carbohydrate metabolism, and an increase in the adaptation period of development, increase safety, increase live weight gain and ensure the improvement of the ecological state of poultry and the environment kg Ambient various Stavropol edge zones.

The technical result is achieved using a biologically active feed additive, including probiotic bifidobacteria of the group Bifidobacterium bifidum in the form of "Bifidumbacterin dry lyophilisate", lactobacillus Lb. Acidophilus in the form of dietary supplements Biobactone dry, dry feed additive from the leafy mass of stevia, grass flour from clover in the following ratio of components in wt.%:

"Bifidumbacterin dry lyophilisate" 70.0 Supplement Biobactone dry 10.0 Dry stevia feed supplement 10.0 Clover Herbal Flour 10.0

which is introduced into the main feed in an amount of 1%.

The essence of the invention lies in the fact that for the preparation of biologically active feed additives for broiler chickens of bifidobacterium group Bifidobacterium bifidum in the form of "Bifidumbacterin dry lyophilisate", lactobacillus Lb. Acidophilus in the form of a dietary supplement Biobacton dry is mixed with a feed additive from the leaf-stem mass of stevia, grass flour from clover and introduced into the main feed for broiler chickens in an amount of 1.0%.

Tests show that the proposed biologically active feed supplement compensates for the deficiency in the diets of protein and carbohydrate biocomponents, useful microorganisms necessary for growth and development, contributing to an increase in the enzymatic activity of the gastrointestinal tract, improving protein-carbohydrate metabolism, and leads to an improvement in the productive performance of chickens .

Using the proposed biologically active feed additive for broiler chickens will allow you to get an effect due to the normalization of microbiocenosis, increased feed digestibility due to increased activity of the gastrointestinal tract, improved protein-carbohydrate metabolism, which will lead to an increase in live weight gain, increased preservation and improved environmental condition of the bird and the environment environment of various zones of the Stavropol Territory.

DETAILED DESCRIPTION OF THE INVENTION

Examples of specific performance of obtaining biologically active feed additives.

Example No. 1

For the preparation of 100 kg of biologically active feed additives are mixed, wt.%: "Bifidumbacterin dry lyophilisate" - 85.0, dietary supplement Biobactone dry - 5.0, dry feed additive from Stevia leaf mass - 5.0, herbal flour from clover - 5 , 0 and contribute to the main feed in an amount of 1%.

Analysis of experimental data on the use of feed additives obtained according to example No. 1 showed an increase in productive indicators: the safety of broilers by 15.7-16.2%, average daily gain by 18.7-20.3%, payment of feed by 4.0 -4.3%.

Example No. 2

A biologically active feed additive is obtained analogously to example No. 1, but for the preparation of 100 kg of a feed additive is mixed, wt.%: Bifidumbacterin dry lyophilisate "- 70.0, dietary supplement Biobacton dry - 10.0, dry feed additive from leaf-stem mass of stevia - 10, 0, herbal flour from clover - 10.0 and contribute to the main feed in an amount of 1%.

The analysis of experimental data on the use of the feed additive obtained according to example No. 2 showed an increase in productive indicators: the safety of broiler chickens by 23.7-24.8%, average daily gain by 25.7-28.3%, payment of feed by 4, 3-4.6%.

Example No. 3

A biologically active feed additive is obtained analogously to example No. 1, but for the preparation of 100 kg of a feed additive is mixed, wt.%: Bifidumbacterin dry lyophilisate "- 70.0, dietary supplement Biobacton dry - 5.0, dry feed additive from the leaf-stem mass of stevia - 10, 0, herbal flour from clover - 15.0 and contribute to the main feed in an amount of 1%.

Analysis of experimental data on the use of biologically active feed additives obtained according to example No. 3 showed an increase in productive indicators: the safety of broiler chickens by 23.5-24.3%, average daily gain by 24.8-27.9%, payment of feed by 4, 2-4.8%.

Control group: The diet of the control group consists without the use of additives.

The invention in comparison with the analogue and other known technical solutions has the following advantages:

The combination of cultures of bifidobacteria of the group Bifidobacterium bifidum in the form of "Bifidumbacterin dry lyophilisate" and Lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton dry provides:

- improvement of the enzymatic, bifidogenic and acidogenic activity of the gastrointestinal tract, normalization of microbiocenosis.

The presence of a vegetable feed additive from the leafy mass of stevia provides:

- enrichment with carbohydrate substances;

- environmental cleanliness.

In general, a biologically active feed additive provides:

- normalization of poultry microbiocenosis and the environment;

- the safety of broiler chickens;

- gain in live weight;

- increase in productive indicators;

- improving the ecological condition of animals and the environment;

- reduction in feed payment.

COMPONENTS OF BIOLOGICALLY ACTIVE FEED ADDITIVES FOR BROILER CHICKENS

PROTEIN FOOD

Full-fledged feeding of the bird is necessary for the manifestation of genetically determined productivity in it, the effective conversion of feed nutrients into products.

Proteins are all nitrogenous feed substances: proteins, amino acids, amides and others. The main component of proteins is a protein of both plant and animal origin, consisting of amino acids that provide growth and development, reproduction, synthesis of hormones and enzymes. Protein is a source of energy, carbohydrates and fat are formed from it.

Essential amino acids are a plastic material from which enzymes and hormones are synthesized, as well as a specific animal protein that contains a set of amino acids characteristic of each bird species.

Carbohydrates provide caloric nutrition of poultry, supplying 60-70% of the required energy. Vegetable feed contains in the form of simple and complex carbohydrates: sugar, starch, pectin, fiber. The content of sugars and pectin in the feed consumed by poultry feed is insignificant, therefore, they do not play a significant role in its nutrition.

The main important carbohydrate for poultry - starch is hydrolyzed in the body to glucose, providing the whole body with it.

BIOLOGICALLY ACTIVE VEGETABLE COMPONENTS - DRY FODDER ADDITIVE FROM STEVIA SHEET

The normal development of a living organism is a rather complex process and a large number of different components and nutrients are needed to maintain it. For the normal development of the poultry organism, biologically active plant components are necessary.

Cultivated in the Stavropol Territory, a new technical culture - honey grass - Stevia (Stevia Rebaudiana Bertoni) has environmental cleanliness and biological activity, as it contains active biologically active substances - fat, protein, fiber, as well as minerals - calcium, phosphorus, Bev, carotene, amino acids - interchangeable and irreplaceable (table. 1).

Table 1 Physico-chemical characteristics of the feed additive from the leaf-stem mass of stevia Name of indicator Mass fraction ND on test methods Crude protein,% not less than 10.0 GOST 13496.4-93 Crude fiber,% no more 24.0 GOST 13496.2-91 Crude ash insoluble in Hcl,% not less 11.6 GOST 26226-95 Crude fat,% not less 1,02 GOST 13979.2-94 Moisture,% no more 10.12 GOST 13496.3-92 Calcium,% not less 1,08 GOST 26570-95 Phosphorus,% not less 0.87 GOST 26657-97 Bev 43.39 GOST 27548-97 Glycosides,% 4.4-4.6 TU 10.18 USSR-76-87 Carotene,% not less 16.25 GOST 27548-97 Permissible mass fraction of nitrates, mg / kg, not more than 200 GOST 13496.19 The amount of exchange energy, MJ, not less 10.68 GOST 27978 Number of feed units, not less 0.9 GOST 7978

In order to use stevia as a source of increasing the ecological status of weakened poultry, to develop energetically and biologically active feed additives of a new generation using plant components, studies were conducted to determine the ecological purity of the stevia plant. It has been established that in the leaves and stems of the stevia variety Ramonskaya sweetened, cultivated in the Stavropol Territory, the content of lead, arsenic, mercury, cadmium, HCH, DDE, DDT, nitrates, etc. is within the limits allowed by SanPiN for plant-based dietary supplements.

The safety indicators of dry leafy mass from stevia correspond to the requirements presented in table. 2.

table 2 Safety indicators of a feed additive from leaf-stem mass of stevia Indicators Allowed Levels Arsenic, mg / kg 0.5 Mercury, mg \ kg 0.05 Lead mg / kg 3.0 Cadmium mg / kg 0.3 Radionuclides: cesium -137 200 strontium - 90 one hundred General toxicity Not allowed Pesticides HCST 0.2 DDT and its metabolites 0.05

Satisfying the physiological needs of poultry in minerals depends on their bioavailability from various feed additives. Vegetable feed consumed by animals only partially satisfies the requirements for some mineral substances, for example, calcium - by 20-30%, phosphorus - by 30-45%. Therefore, for proper feeding, it is necessary to additionally introduce in the diet of animals supplements containing macro- and microelements.

Table 3 shows the microelement composition of the leaves and stems of stevia.

Table 3 The trace element composition of leaves and stems of stevia, mg / kg No. p.p. Name Manganese Zinc Copper one. Leaves 14.0 33.8 7.0 2. The stalks 4.0 7.0 4.2

As a result of studies of the microelement composition of leaves and stems of stevia, data were obtained that the leaves of stevia in comparison with stems contain 3.5 times more manganese, 4.8 times more zinc, almost 2 times copper.

The content in the leaves of stevia of manganese, copper and zinc determines its therapeutic effect in a number of diseases of the bird and as a corrector of immunity.

The leaves of stevia contain a complex of biologically active substances that determine the adaptogenic properties of the body. In the complex of modern problems, stevia has properties useful for the bird’s body that are necessary for creating new generation feed additives and can become a promising culture not only in agronomic practice, but also in animal husbandry.

BIOLOGICALLY ACTIVE ADDITIVES - PROBIOTICS IN THE FORM OF LIVING BIOCULTURES OF MICRO-ORGANISMS

The effectiveness of animal feed use depends on a number of factors, including the conditions of keeping and feeding, as well as their genetic potential. To partially replace the missing biologically active substances that stimulate the growth, development and vital activity of the body, as well as normalize the biocenosis, it is necessary to use dietary supplements of microbial origin. In countries with highly developed livestock breeding, probiotics are widely used to prevent intestinal dysbiosis of young farm animals and birds.

Biologically active substances - probiotics are a new class of drugs that affect the body at a systemic level. Their influence affects regulatory systems, due to which nonspecific resistance of the organism, immunity is activated.

Acidophilic lactobacilli in the form of dietary supplement “Biobactone dry” TU 9229-054-07532800-2005 contain up to 10 ⁹ bacterial cells of Lb. Acidophilus strain 12 b. This strain of acidophilic lactobacilli is safe: it contains live microorganisms that "colonize" the digestive tract and have the ability to inhibit or inhibit the growth of bacteria that cause gastrointestinal upset. By the mechanism of action of the dietary supplement “Biobacton dry” compensates for the deficiency of acidophilic rods and has a high antagonistic activity against a wide range of pathogenic and conditionally pathogenic microorganisms, which leads to the displacement of the latter from intestinal microbiocenosis and the creation of favorable conditions for normalizing microflora and digestive tract function, metabolic processes.

Bifidobacteria of the Bifidobacterium bifidum group in the form of the immunobiological preparation “Bifidumbacterin dry lyophilisate” have a bifidogenic effect and provide activation of the immune system, the formation of non-specific immune bodies, lower intestinal pH, and create unfavorable conditions for the development of pathogenic microflora.

In general, the combination of components of a biologically active feed additive for broiler chickens: probiotics - live cultures of lactobacilli, bifidobacteria and vegetable dietary supplements - a feed additive from the leafy stem mass of stevia in their composition provides an increase in reproductive qualities and productive indicators.

Claims (1)

Dietary supplement for broiler chickens, including bifidobacteria of the group Bifidobacterium bifidum in the form of "Bifidumbacterin dry lyophilisate", lactobacillus Lb. Acidophilus in the form of dietary supplement Biobacton is dry, characterized in that it additionally contains a dry feed additive from the leaf-stem mass of stevia, herbal flour from clover, in the following ratio of components, wt.%:
Bifidumbacterin dry lyophilisate 70 Supplement Biobactone dry 10 dry feed supplement from Stevia leaf mass 10 clover grass meal 10
and is introduced into the main feed in an amount of 1%.