RU2759845C1 - Method for reducing endogenous losses of macroelements in the body of broiler chickens - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to the agricultural industry, in particular, to a method for reducing endogenous losses of calcium, potassium and magnesium in the pectoral muscles, and calcium, sodium in the femoral muscles in broiler chickens. The method is characterised by the fact that broiler chickens on a mineral-deficient diet are administered the Bifidumbacterin preparation per os once a day at a dosage of 1.0 g/kg of feed.

EFFECT: use of the invention will provide a possibility to maintain the level of calcium, potassium, magnesium, sodium in the body of chickens.

1 cl, 2 tbl

Description

The invention relates to the agricultural industry and can be used in the cultivation of poultry, in particular broiler chickens.

The purpose of the present invention is to develop a method for reducing endogenous losses of macronutrients in the body of broiler chickens.

A known method of reducing endogenous losses of cobalt, iodine and zinc from the body of animals, characterized by the fact that rats of the Wistar line per os once a day inject the drug "Lactobifadol" based on lactic acid bacteria Lactobacillus acidophilus LG1-DEP-VGNKI and bifidobacteria Bifidobacterium Bifidobacter -DEP-VGNKI at a dosage of 0.3 g / kg of live weight and a mixture of antiquorum substances of Quercus cortex extract in an amount of 500 ml for 4 weeks of the experiment per one head on an empty stomach, while the mixture of antiquorum substances contains active components per 1 ml of water: vanillin - 0.053 mg, propylresorcinol - 0.138 mg, coniferyl alcohol - 0.45 mg, coumarin - 0.048 mg, scopoletin - 0.03 mg and antiarol - 0.179 mg [1].

The disadvantage of this method is that it is aimed at removing only cobalt, iodine and zinc, as well as the introduction of Quercus cortex extract into the body leads to an imbalance in the normal flora and pathogenic microflora in the animal body.

Experimental studies were carried out in the conditions of the Center for Shared Use of Scientific Equipment of the Federal Scientific Center of the BST of the Russian Academy of Sciences on broiler chickens of the Arbor Aikres cross of Orenburgskaya Poultry Farm. Experimental studies were carried out on 30 heads of 7-day-old broiler chickens (2 groups, n = 15). During the experiment, all birds were kept in the same conditions. During the preparatory period (within 14 days), broiler chickens were on a full-fledged basic diet, taking into account the recommendations of VNITIP [2]. The microclimate in the room met the requirements of VNITIP.

After 14 days of the preparatory period, the groups were transferred to a diet deficient in minerals. The control group received an experimental diet consisting of cooked polished rice (boiling rice in distilled water for 15 minutes, followed by removing the broth and rinsing), supplemented with vitamins A, D, C, K, E and B vitamins, determined by the recommendations of the Institute of Nutrition. RAMS [3, 4]. Drinking was carried out with distilled water. The experimental group received an experimental diet with the additional inclusion of the probiotic preparation Bifidumbacterin (powder) at a dosage of 1.0 g per kg of feed per day [5, 6].

The keeping of the poultry and the procedures during the experiments complied with the requirements of the instructions and recommendations of the Russian regulations (Order of the Ministry of Health of the USSR ¹ 755 of 08/12/1977) and "The Guide for Care and Use of Laboratory Animals (National Academy Press, Washington, DC, 1996)".

The keeping of the poultry and the procedures during the experiments complied with the requirements of the instructions and recommendations of the Russian regulations (Order of the Ministry of Health of the USSR ¹ 755 of 08/12/1977) and "The Guide for Care and Use of Laboratory Animals (National Academy Press, Washington, DC, 1996)".

The elemental composition of biosubstrates of broiler chickens was studied using atomic emission and mass spectrometry with inductively coupled argon plasma. Additional studies were carried out in the laboratory of ANO Center for Biotic Medicine, Moscow (accreditation certificate GSEN.RU.ЦОА.3.11, ROSS RU.0001.513118) (ICAP-9000 devices "Thermo Jarrell Ash, USA, Perkin Elmer Optima 2000 DV, USA ").

In the course of the studies, it was found that at the end of the experiment in the groups with a mineral deficient diet, the elimination of all macronutrients was observed in comparison with the group on a full diet.

However, it should be noted that the additional inclusion of the Bifidumbacterin preparation based on the Bifidobacterium bifidum strain at a dosage of 1.0 g per kg of feed per day has a positive effect on the content of macronutrients in the body of broilers in comparison with the control group on a mineral deficient diet.

From the data in Table 1, it follows that the additional inclusion of the Bifidumbacterin preparation based on the Bifidobacterium bifidum strain at a dosage of 1.0 g per kg of feed per day contributes to a significant increase in macronutrients in the breast muscles of broiler chickens: calcium by 1.8 times (p≤0.01 ), potassium by 1.7 times (p≤0.01), magnesium by 1.5 times (p≤0.05) and 1.7 times (p≤0.01), in comparison with the control group. The phosphorus level exceeded the control group by 1.4 times, but the changes were insignificant.

From the data in Table 2, it follows that the content of macroelements in the thigh muscles of broiler chickens in the groups on a mineral deficient diet at the end of the experiment decreased in comparison with the group at the beginning of the experiment. However, the additional inclusion of the Bifidumbacterin preparation based on the Bifidobacterium bifidum strain at a dosage of 1.0 g per kg of feed per day also contributes to a significant increase in calcium by 2.0 times (p≤0.01) and sodium by 1.5 times (p≤0, 05), in comparison with the control. The content of potassium, magnesium and phosphorus also increased, but the changes were not significant.

In general, for the entire period of the experiment, the additional inclusion of the Bifidumbacterin preparation based on the Bifidobacterium bifidum strain at a dosage of 1.0 g per kg of feed per day in the diet of broiler chickens has the best performance in comparison with analogs from the control group.

Sources of information

1. Patent for invention RU No. 2700617. A way to reduce endogenous losses of essential elements (Co, I, Zn) from the body of animals / G.K. Duskaev, S.A. Miroshnikov, O.V. Kwan, I.A. Gavrish, Sh.G. Rakhmatullin, B.S. Nurzhanov, B.G. Rogachev, O. A. Zavyalov, A.N. Frolov: published on September 18, 2019. Bul. No. 26.

2. Recommendations for feeding poultry / Fisinin V.I., Imangulov Sh.A., Egorov I.A., Okolelova T.M. and others - Sergiev Posad, 2000 .-- 67 p.

3. Kwan OV Endogenous loss of mineral substances from the body of animals under the influence of Bifidobacterium longum / O.V. Kwan // Trace elements in medicine. - 2008. - No. 9 (1-2). - S. 18.

4. Kwan OV Influence of probiotic preparations on the content of elements in the body of laboratory animals / O.V. Kwan, E.A. Rusakova, A.N. Sizentsov, V. Ya. Kataev // Bulletin of the Orenburg State University. - 2014. - No. 6 (167). - S. 64-66.

5. Miroshnikov S.A. Influence of oral administration of Bifidobacterium longum on the value of endogenous losses of heavy metal ions. Miroshnikov, O.V. Kwan, D.G. Deryabin, S.V. Notova // Bulletin of the Orenburg State University. - 2005. - No. 2S -2 (40). - S.44-46.

6. Miroshnikov S.A. The role of normal microflora in the mineral metabolism of animals / S.A. Miroshnikov, O.V. Kwan, B.S. Nurzhanov // Bulletin of the Orenburg State University. - 2010. - No. 6 (112). - S. 81-83.

Claims (1)

A method of reducing endogenous losses of calcium, potassium and magnesium in the pectoral muscles, and calcium, sodium - in the thigh muscles of broiler chickens, characterized by the fact that broiler chickens on a diet deficient in minerals are administered the drug Bifidumbacterin per os once a day at a dosage of 1.0 g / kg feed.