RU2796271C1 - Method for selectively reducing the content of toxic elements in the body of broiler chickens - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention for agriculture, in particular for a method for selectively reducing the content of toxic elements in the body of broiler chickens. The method is characterized by the fact that 7-day-old broiler chickens on a semi-synthetic diet deficient in trace elements are injected with ultrafine iron particles at a dosage of 2.5 mg per kg of feed per day during a growing period of 35 days.

EFFECT: use of the invention will reduce the level of strontium, cadmium, tin and aluminum in the body of chickens.

1 cl, 3 tbl

Description

The invention relates to agriculture and can be used in the cultivation of poultry, in particular broiler chickens.

The purpose of the proposed invention is to develop a method for selectively reducing the content of toxic elements in the body of broiler chickens.

A known method for reducing endogenous losses of cobalt, iodine and zinc from the body of animals, characterized in that the Wistar per os rats once a day are injected with the drug "Lactobifadol" based on lactic acid bacteria Lactobacillus acidophilus LG1-DEP-VGNKI and bifidobacteria Bifidobacterium adolescentis B-1 -DEP-VGNKI at a dose of 0.3 g/kg of live weight and a mixture of antiquorum substances of Quercus cortex extract in an amount of 500 ml for 4 weeks of the experiment per head on an empty stomach, while the mixture of antiquorum substances contains active components per 1 ml of water: vanillin - 0.053 mg, propylresorcinol - 0.138 mg, coniferyl alcohol - 0.45 mg, coumarin - 0.048 mg, scopoletin - 0.03 mg and antiarol - 0.179 mg [1].

The disadvantage of this method is that it is aimed at removing only cobalt, iodine and zinc, and the introduction of Quercus cortex extract into the body leads to an imbalance in the normal flora and pathogenic microflora in the animal body.

Experimental studies were carried out in the conditions of the Center for the Collective Use of Scientific Equipment of the Federal Scientific Center of the BST RAS on broiler chickens of the Arbor Aikres cross of OAO Orenburg Poultry Farm. Experimental studies were carried out on 90 heads of 7-day-old broiler chickens (3 groups, n=30). By the method of pairs-analogues, 3 groups were formed: two control (K ₁ , K ₂ ) and one experimental group. The control group (K ₁ ) was on a semi-synthetic diet (PR) fully balanced in trace elements, K ₂ received a semi-synthetic diet deficient in microelements (PRDM), the experimental group - PRDM with the additional inclusion of an ultrafine particle (UFP) of iron at a dosage of 2.5 mg / kg of feed (Table 1). During the experiment, all birds were kept in the same conditions, the microclimate in the room met the requirements of VNITIP [2].

The control group received a semi-synthetic diet (Table 2) consisting of boiled polished rice (cooking the rice in distilled water for 15 minutes, then removing the decoction and rinsing), supplemented with vitamins A, D, C, K, E and B vitamins. determined by the recommendations of the Institute of Nutrition of the Russian Academy of Medical Sciences [3]. Drinking was carried out with distilled water.

The preparation of ultrafine particles of iron was carried out in isotonic solution by ultrasonic treatment of an accurate sample under the conditions: f-35 kHz, N-300 W, A-10 μA, for 30 minutes. After carrying out the dispersion of UHF with a hydrodynamic radius of 388±37 nm in the appropriate concentration, by stepwise mixing with the diet.

The keeping of poultry and the procedures during the experiments complied with the requirements of the instructions and recommendations [4, 5].

To study the selective reduction of toxic elements in the body of broiler chickens at the age of 42 days, one slaughter was carried out. Slaughter was carried out according to the established method [6]. The analysis of the studied biosubstrates was carried out in the laboratory of the ANO Center for Biotic Medicine, Moscow (accreditation certificate GSEN.RU.TsOA.311, registration number in the state register ROSS RU.0001.513118 dated May 29, 2003) using the methods of atomic emission and mass spectrometry with inductively coupled argon plasma (instruments ICAP-9000 "Thermo Jarrell Ash", USA, Perkin Elmer Optima 2000DV, USA). Sample preparation was carried out in accordance with recommendations 4.1.1482-03 and 4.1.1483-03, by microwave decomposition on a Multiwave 3000 instrument.

According to the content of toxic elements in the body of broiler chickens. the following results were obtained (Table 3).

Based on the results of the obtained data, a significant decrease in strontium in the body of the bird in the experimental group was revealed, when compared with K ₁ by 21.6%, in relation to K ₂ by 17.9% (p≤0.05). The level of cadmium in the experimental group decreased by 14.3%, when conducting a comparative analysis with K ₂ . There was a significant decrease in tin by 2.6 and 2.4 times (p≤0.01) and aluminum by 2.67 and 1.85 times (p≤0.05), respectively. The level of lead was reduced in the experimental group by 20.3% relative to K ₁ .

The technical result is a selective reduction in the content of toxic elements in the body of broiler chickens.

Information sources:

1. Patent for invention RU No. 2700617 Method for reducing endogenous losses of essential elements (Co, I, Zn) from animals / G.K. Duskaev, S.A. Miroshnikov, O.V. Kwan, I.A. Gavrish, Sh.G. Rakhmatullin, B.S. Nurzhanov, B.G. Rogachev, O.A. Zavialov, A.N. Frolov: published on 09/18/2019. Bull. No. 26.

2. Guidelines for the technology and breeding of poultry - Sergiev Posad, VNITIP, 2008. - 48 p.

3. Patent for invention RU No. 2759845 Method for reducing endogenous losses of macronutrients in the body of broiler chickens / O.V. Kwan, A.V. Bykov, S.A. Miroshnikov, G.K. Duskaev, Sh.G. Rakhmatullin, E.A. Sizova, E.V. Sheida: published 11/18/2021. Bull. No. 32.

4. Order of the Ministry of Health of the USSR dated August 12, 1977 No. 755 "On measures to further improve the organizational forms of work using experimental animals."

5. The Guide for Care and Use of Laboratory Animals - National Academy Press, Washington, D.C., 1996, 246 p.

6. Guidelines for scientific research on the feeding of poultry. Ed. Fisinina - Sergiev Posad: VNIITIP, 2008. - 25 p.

Table 1 Scheme of the experiment to assess the effect of UHF iron on the body of broiler chickens Group Number of goals in groups Feeding conditions K ₁ thirty Semi-synthetic diet (SR) K ₂ Semi-synthetic micronutrient deficient diet (MTD) Experienced PRDM + UHF Fe

table 2 Ingredient Composition of diets, g/100 g feed ETC PRDP Casein 20 20 Gelatin 5 5 Cellulose 3 3 Vegetable oil 3 3 Choline chloride 0.2 0.2 Glucose 1.25 1.25 Rice 61.38 61.38 Methionine 0.1 0.1 cystine 0.2 0.2 CaHPO ₄ *H ₂ O 1.8 1.8 _CaCO3 1.45 1.45 _{KH2PO4 _} 1.013 1.013 KCl 0.21 0.21 _{Na2CO3 _} 0.555 0.555 MnCl*4H ₂ O 0.04 - FeSO ₄ * 7H ₂ O 0.05 - MgSO ₄ * 7H ₂ O 0.615 0.615 KJ 0.001 0.001 CuSO ₄ * 5H ₂ O 0.001 - ZnCl ₂ 0.016 - _CoCl2 0.0002 - NaMoO ₄ *2H ₂ O 0.0008 - Na ₂ SeO ₃ 0.000015 - Vitamin Blend* 0.052 0.052 * Vitamin mixture (mg per 100 g of feed): B ₁ - 2.5; B ₂ - 1.5; _B6 - 0.6; B ₁₂ - 0.002, Ca-pantothenate - 2.0; biotin - 0.06; folic acid - 0.4; K ₃ - 0.5; C - 25.0; PP - 15.0; A 1000 IE; D ₃ - 360 IE; E - 0.5 IE

Table 3 Element The content of toxic elements in the body of broiler chickens, mg/kg K ₁ K ₂ experienced Sr 29.2±1.31 27.9±1.87 22.9± ^1.75ab CD 0.12±0.001 0.14±0.0012 0.12±0.0011 sn 0.13±0.001 0.12±0.0011 0.05± ^0.0012ab Pb 1.18±0.63 0.67±0.03 0.94±0.14 Al 1.63±0.74 1.13±0.68 0.61± ^0.14ab

Note:

a - significant changes with K ₁ (p≤0.05);

b - significant changes with K ₂ (p≤0.05).

Claims (1)

A method for selectively reducing the content of toxic elements of strontium, cadmium, tin and aluminum in the body of broiler chickens, characterized in that 7-day-old broiler chickens on a semi-synthetic diet deficient in trace elements are injected with ultrafine particles of iron at a dosage of 2.5 mg per kg feed per day during a rearing period of 35 days.

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