RU2376891C1 - "vitapant" balsam - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention is related to the field of food industry and preventive medicine. Antlers and magnesium balsam contains alcohol-free extract made of unossified antlers, carbohydrates, magnesium sulphate, alcohol-free vegetable concentrated tonic extract and water. Alcohol-free vegetable extract is rootstocks and roots of Rhaponticum carthamoides or ginseng roots or Manchurian aralia roots. Balsam contains components in following ratio, wt %: alcohol-free antlers extract 2.0-6.0, alcohol-free vegetable tonic concentrated extract 0.5-1.5, magnesium sulphate 4.0-7.0, carbohydrates 25.0-45.0, water - the rest.

EFFECT: invention allows to obtain biologically active additive - balsam in fluid form with no alcohol containing components with increased immunomodulating activity.

6 cl, 1 tbl, 6 ex

Description

The invention relates to food additives and balms as a component that is part of the functional nutrition of a person, and can be used in the food industry and preventive medicine.

It is known to use magnesium sulfate as a dietary supplement for functional nutrition and the prevention of human diseases, for example, in the natural sulfate-magnesium mineral waters "Jermuk", "Smirnovskaya", "Essentuki-17" with the content of magnesium ions, which actively affect the gall-excretory function liver, enhance cystic reflux, change the physico-chemical properties of bile, increase the content of bile acids. These properties of sulfate-magnesium waters are used to prevent biliary dyskinesia, cholangitis, peptic ulcer of the duodenum and stomach (Bogomolov V.M. Handbook of the sanatorium resort. Selection. 1986, p. 38).

However, the mineral waters mentioned above have a narrow spectrum of functional effects on the human body, and the content of magnesium ions in these waters is less than 1.0 g / l.

The known composition of ingredients for balm contains per 1000 decalitres: 0.5-1.0 kg of aloe, 0.8-1.0 kg of lemon flowers, 4.0-6.0 kg of feijoa fruits, 3.0-5.0 kg fig leaves, 4.0-6.0 walnut leaves, 12.0-16.0 kg of tea, 4.0-6.0 kg of blackberry leaves, 4.0-6.0 kg of Eleutherococcus, 2.0-4 , 0 kg of Rhodiola, 2.0-3.0 kg of oak bark, 80.0-100.0 L of red wine, 4.0-6.0 kg of persimmon leaves, 2.0-4.0 kg of rhododendron leaves, 720 , 0-800.0 kg of honey, 0.5-0.7 kg of propolis, 0.10-0.15 kg of mummy, 500.0-700.0 kg of colour, 3.6-4.2 kg of citric acid, 0.8-1.0 kg of fragrant olive flowers, 1.2-1.4 kg of ginseng, 2.0-4.0 kg of kidney tea, 3.0-5.0 kg of half-grass (Erva woolly) grass, 0, 10-0.15 kg of bee flower pollen, 200.0-300.0 l of apple juice, 0.8-1.0 kg of antlers of sika deer or deer and water-alcohol liquid - the rest (RF Patent №2018519, IPC A23L 1/30, A23L 1 / 08, A61K 35/32, published on August 30, 1994).

A known composition for the preparation of semisweet tinctures, in which the following components are used, kg / 1000 gave tinctures: sugar 1200-1300, pantocrine 90-110, oak bark 18-20, dry leaf of frankincense 14-16, red pepper 1.4-1 , 6, St. John's wort 3.7-3.9, wormwood 0.6-0.8, lobaznik 4.4-4.6, pine cone with nut 14-16, yarrow 0.6-0.8, citric acid 1 , 43-1.45 and the rest of the aqueous-alcoholic liquid (RF Patent No. 2031927, IPC C12G 3/06, publ. 03/27/1995).

A known composition of ingredients for a balsam containing a golden root, cherry fruits, pine nut, tansy, moral root, birch buds, oregano, peppermint, chamomile, honey, color and water-alcohol liquid, characterized in that it additionally contains frankincense leaves thick-leaved, yarrow, succession, leaf of blackcurrant, root of the peony evading, root of calamus marsh, coriander, root of elecampane, antlers of maral, rosehip infusion, sea buckthorn spirit juice, bird cherry fruit in the following ratio of ingredients, g per 1000 dal of finished product: golden root 2.0-2.5; cherry fruits 20.0-35.0; pine nut 30,0-35,0; tansy 0.5-1.0; morality root 0.5-1.0; birch buds 3.0-4.0; oregano 1.0-1.5; peppermint 2.0-2.5; pharmacy chamomile (flowers) 4.0-5.0; leaves of frankincense 5.0-6.0; yarrow 5.0-6.0; a series of 3.0-3.5; black currant leaf 4.0-5.0; pion root evasive 0.8-1.0; calamus root marsh 2.0-2.5; elecampane root 5.0-6.0; coriander 2.0-2.5; deer antlers 0.2-0.5; honey 70-280; Kohler 400-850; and also in l: sea-buckthorn alcoholized juice 1100-1250; rosehip infusion 50-60; bird cherry fruit 200-210; sugar syrup (70%) 200-300; water-alcohol liquid - the rest (RF Patent No. 2077563, IPC C12G 3/06, publ. 04/20/1997).

Known alcohol-free food supplement in liquid form containing biologically active substances, which is a sugar-like antler extract, obtained by extraction of the antlers with sugar-like syrup, mainly flower honey, at a temperature of a "water bath" (RF patent No. 2177700, IPC A23L 1/30, A23L 1 / 08, A61K 35/32, published on January 10, 2002). However, this food supplement has an insufficient spectrum of nutrients due to the low extracting ability of sugar-like syrups.

A known composition for food supplement balm "Ginseng-Vita" containing the following ingredients, kg / 1000 gave the finished product: manchurian waxy ripeness 20.0-22.0, pine nut 40.0-42.0, oak bark 17.5 -18.0, magnolia vine seeds 1.0-1.2, birch buds 2.5-3.0, cinnamon 0.5-0.6, natural honey 100.0-105.0, sugar 50.0-52 , 0, citric acid 0.4-0.5, and also, l: ginseng tincture 18.0-20.0, hydrolyzed salmon milk 10.0-10.5, 20% grape juice 100.0- 105.0, cognac 320.0-350.0, water-alcohol liquid - the rest. This allows you to expand the range of food additives - balms (RF Patent No. 2113135, IPC A23L 1/29, A23L 1/30, C12G 3/06, publ. 06/20/1998).

A known composition of the ingredients for the balm "Relict", containing, kg per 1000 l of the finished product: rosehips 42.6-55.3, leaves of Eleutherococcus 10.7-12.0, fruits of aronia 45.6-53.2, roots Manchurian Aralia 2.2-2.5, antler sika or reindeer, or deer, or red deer 1.2-1.9, color 48.0-50.0, sugar 158.0-168.0, salt 2 , 0-2.3, water-alcohol liquid - the rest. The invention allows to expand the range of balms of therapeutic and preventive action (RF Patent No. 21115712, IPC C12G 3/06, A23L 2/56, publ. July 20, 1998).

Known therapeutic and preventive food supplement-balm "Elixir" containing the following ingredients, kg / 1000 gave the finished product: thyme herb 20.0-22.0, fruits of pine nuts 20.0-25.0, fruits of hawthorn 50.0- 55.0, rose hips 50.0-55.0, rose hips 10.0-12.0, wild strawberry 10.0-15.0, catnip lemon 20.0-25.0, rowan fruits 50 , 0-52.0, Volodushka 2.0-3.0, grass and stems of wormwood, 1.0-2.0, roots of the mountaineer snake 1.0-2.0, flowers of the apple tree 5.0-6.0, chaga 20.0-25.0, clove 0.2-0.3, trepang 2.0-2.5, blackhead grass 1.0-2.0, grass and roots of bitterness 0.5-1.0, seeds go Sarepta rychitsa 0.1-0.15, propolis 0.3-0.4, and also, l: hydrolyzed salmon milk 35.0-40.0, tincture of sika deer 10.0-11.0, ginseng tincture 5.0-5.5, natural honey 200.0-220.0, water-alcohol liquid - the rest. Elixir balm is used as a food additive in tea, coffee, mineral water to provide a comprehensive therapeutic and preventive effect (RF Patent No. 2123038, IPC C12G 3/06, A23L 1/30, publ. 10.12.1998).

A known composition of ingredients for the Panta Forte balm containing pantocrine, dry leaf of incense, oak bark, chilli pepper, St. John's wort, inflorescences, yarrow grass, lewsee root and rhodiola extract in the following ratio of ingredients, kg / 1000 gave: pantocrine 0.5-500.0; dry leaf of frankincense 1,0-1300,0; oak bark 1.0-1300.0; chilli pepper 0.05-50.0; St. John's wort grass 1.0-1300.0; inflorescences of meadowsweet 0.3-300.0; yarrow herb 0.05-50.0; Leuzea root 0.05-50.0; Rhodiola extract 0.01-100.0; water-alcohol liquid - the rest. The invention provides increased biological activity, improved organoleptic characteristics by introducing new ingredients and simplifying the composition (RF Patent No. 2135567, IPC C12G 3/06, A61K 35/78, publ. 08/27/1999). The invention can be used in the food industry, medicine, in particular in preventive pharmacology, and relates to agents that improve mental and physical performance of both healthy and sick people.

Known balm "Yenisei" (RF application No. 97102269, IPC C12G 3/06, publ. 05/10/1999). The Yenisei balm contains extracts of medicinal plants and animal raw materials dissolved in strong alcohol: deer antlers, horsetail, oregano leaves, wormwood, bison, St. John's wort, yarrow, sweet clover, roots of a lewsee, galangal, angelica, anise star fruit - star anise, coriander, fennel, bird cherry branches, black tea, rose and lemon oil, cognac, sugar syrup, alcohol 45%.

However, all of the above analogues - food additives - balms in liquid form contain ethyl alcohol in high concentrations as a preservative and extractant of biologically active substances, which limits the scope of use of these products by people who have contraindications for the use of ethyl alcohol.

Known composition for the preparation of food additives in the form of dragees (RF patent No. 2100938, IPC A23G 3/00, publ. 10.01.1998), containing granulated sugar, powdered sugar, molasses, dry vegetable additive, vegetable oil, beeswax and water, characterized in that it additionally contains maral antlers crushed to a powder state and maral antler meal, natural honey, talc and food coloring selected from the tartrazine group, crimson 4P granular C1 1625 E 124 or a mixture thereof in any ratio, and as dry herbal supplement crushed grass of oregano, peppermint leaves and frangipani leaves in the following ratio of components (kg per 1 ton of finished product):

Sugar 100-110

Icing sugar 799-809

Molasses 50-62

Oregano grass 3.0-3.5

Peppermint Leaves 3.7-4.0

Leaves of frankincense 3.0-3.4

Antlers of the maral 2.8-3.2

Meal of antlers of maral 15.0-15.3

Natural honey 18.0-19.0

Food color 0.15-0.17

Vegetable oil 0.2-0.3

Talcum 1.0-1.2

Beeswax 0.18-0.24

Water is the rest.

Dragee is made by forming dragee cases, repeated processing of them with sugar syrup followed by rolling with icing sugar and glossing of the obtained semi-finished product, characterized in that the manufacture of cases is carried out by processing pre-prepared sugar grains 2-3 mm in size with sugar syrup, to which honey is added, and knurling on it are mixtures of dry finely ground leaves of peppermint, frankincense, oregano grass, powdered deer antlers and meal of deer antlers to a build The mass of the shells is 50-70% of the mass of the finished dragee, the further processing of the shells is carried out with sugar-syrup, to which food coloring is added, and powdered sugar, and talc is additionally added to the glossing composition.

However, in such a product, the substances contained in crushed deer antlers and plant leaves are in an insufficient bioavailable state, which reduces the effectiveness of their use.

The closest analogue (prototype) is the “Antlers plus” balm containing sika deer antlers, plant extracts, carbohydrates, mummies, salmon milk hydrolyzate and water-alcohol liquid (RF Patent No. 2093557, IPC C12G 3/06, publ. 20.10 .1997). Balm "Panty plus" has a comprehensive therapeutic and prophylactic effect.

However, such a balm contains ethyl alcohol in high concentrations as a preservative and extractant of biologically active substances, which limits the scope of use of these products by people who have contraindications for the use of ethyl alcohol, as well as an insufficient spectrum of useful biologically active substances.

The technical result of the invention is to obtain a balm without alcohol-containing components with a higher immunomodulating activity of its composition.

The specified technical result is achieved by the fact that the alcohol-free panto-magnesium balm includes an alcohol-free concentrate of the extract from antlers, carbohydrates, magnesium sulfate, alcohol-free plant extract of the tonic effect in concentrated form of at least one species from the group: rhizomes with safflower-root roots, or roots ginseng, or Manchurian aralia roots and water with the following content of components, wt.%: alcohol-free concentrate of the extract from antlers 2.0-6.0; alcohol-free plant extract tonic action in concentrated form 0.5-1.5; magnesium sulfate 4.0-7.0; carbohydrates 25.0-45.0; water is the rest. The balm additionally contains vegetable essential oil that improves the taste of the product in an amount of 0.1-0.3 wt.% And Polysorbate 80 in an amount of 0.5-1.5 wt.%. As a plant essential oil, balm contains clary sage essential oil. The carbohydrate balm contains sugar, or fructose, or honey, or starch syrup, or food inulin from Jerusalem artichoke. The water used in the balm can be treated with shungite.

When combining the use of magnesium sulfate and antler extract together with other components of the claimed balsam, a higher immunomodulatory effect of this product on the body was found compared to formulations without one of these components. The maximum values of the quantitative content of the components of the claimed balsam are justified by its maximum immunomodulating effect, a higher content of which does not give a significant increase in effect and is inappropriate undesirable side effect on organisms burdened, for example, by various diseases of the kidneys or liver. The minimum values of the quantitative content of the balm components are justified by their minimal prophylactic and immunomodulatory effects, whose lower content does not give the desired healing effect.

The alcohol-free concentrate of the antler extract is an extract of antlers, from which the ethyl alcohol extractant has been removed.

Clary sage essential oil has a spicy herbal taste, is used as an antiseptic, anti-inflammatory, analgesic, antispasmodic.

The liquid extract of safflower-root leuzea root has an exciting effect on the central nervous system, which manifests itself in an experiment on various animal species in increasing motor activity and reflex excitability, as well as in enhancing the level of behavioral reactions. In addition, galenic dosage forms have a noticeable awakening property in the case of the use of sleeping pills. The most widely studied tonic properties of safflower levzea. It has been experimentally proved that with a single and repeated administration of Leuzea preparations, the performance of tired animal skeletal muscles clearly increases. It has been established that galenic remedies of leuzea significantly extend the working time before the onset of fatigue and reduce the level of damaging factors during exhausting physical exertion. These properties of leuzea as a means of increasing overall performance were confirmed in animal experiments by the study of biochemical parameters such as ATP, glycogen, and creatine phosphate. Application in medicine. A clinical study of tincture and liquid extract of safflower leuzea was carried out mainly in patients suffering from functional disorders of the central nervous system, as well as in physical and mental overwork, impotence and chronic alcoholism. The greatest therapeutic efficacy of galenic preparations of Leuzea was noted in the treatment of patients with mild manifestations of asthenia, with complaints of increased fatigue, poor mood and decreased appetite, irritability, headache, poor sleep, decreased sexual activity, and various vegetative-vascular disorders. When treating this group of patients, after 10-20 days, their general condition and mood improved, their sleep and appetite returned to normal, and their working capacity increased. In some patients, the vegetovascular sphere returned to normal and an increase in sexual potency was noted, depressive phenomena decreased, and neuropsychic tone increased. There were no side effects from the central nervous system and the cardiovascular system, as well as the negative effect of tincture of the liquid extract of saflovoid levzea on the main indicators of blood and urine in patients with prolonged use of the drugs.

Magnesium sulfate has a sedative, diuretic, arterial dilating, anticonvulsant, antiarrhythmic, hypotensive, antispasmodic effect. It regulates metabolic processes, interneuronal transmission and muscle excitability, inhibits Ca2 + entry through the presynaptic membrane, and reduces the amount of acetylcholine in the peripheral nervous system and central nervous system. It relaxes smooth muscles, reduces blood pressure (mainly increased), increases diuresis. Anticonvulsant effect - Mg2 + reduces the release of acetylcholine from neuromuscular synapses, while inhibiting neuromuscular transmission, has a direct inhibitory effect on the central nervous system. Antiarrhythmic action - Mg2 + reduces the excitability of cardiomyocytes, restores ionic equilibrium, stabilizes cell membranes, disrupts the Na + current, slow incoming Ca2 + current and one-way K + current. The cardioprotective effect is due to the expansion of the coronary arteries, a decrease in OPSS and platelet aggregation. Tocolytic effect - Mg2 + inhibits the contractility of the myometrium (decreased absorption, binding and distribution of Ca2 + in smooth muscle cells), increases blood flow in the uterus as a result of the expansion of its vessels. It is an antidote for poisoning with salts of heavy metals. Systemic effects develop almost instantly after i / v and 1 hour after i / m administration.

Polysorbate 80 (tween 80) is a nonionic surfactant, refers to the monoesters of polyoxyethylated sorbitan (spen) and higher fatty acids, it is soluble in water and used in medicine to stabilize emulsions and suspensions, it is harmless and is recommended for injectable formulations. Polysorbate 80 during distillation of the extractant (ethyl alcohol) ensures the conservation of volatile biologically active substances in the final product, which can be distilled together with the extractant in the absence of polysorbate 80, and increases the bioavailability of biologically active substances when the products are consumed.

Fructose has a very sweet taste (1.5 times sweeter than sucrose and 2 times sweeter than glucose), is well absorbed in the body and is used as a source of carbohydrates for patients with diabetes mellitus.

Inulin (natural fructose), which inulin consists of, is a unique sugar that is able to participate in the same metabolic processes as glucose and fully replace it in situations where glucose is not absorbed by cells. That is why the dietary and therapeutic value of inulin is very high.

Shungite is a universal absorbent and agent that releases various organic and mineral substances upon contact with water. On the surface of shungite, pollutants are destroyed by 80-95% and are fixed. Contact of water with shungite leads to the formation of a water-mineral solution with unique healing properties. Fullerenes combined with water molecules form a new substance, an aqueous solution that can be used for various diseases. Thus, shungite stone purifies water from harmful impurities, dissolved iron, chlorine, organic compounds, structures water, makes it biologically active. Such water helps stimulate, rejuvenate the body, and prevent various diseases. It is recommended to use in complex therapy for allergies, skin diseases, intoxications, infectious diseases, with a decrease in immunity, diseases of the digestive tract, airborne diseases, metabolic disorders.

The manufacturing technology of dietary supplements "Panto-magnesium balsam" Vitapant ""

Production consists of the following stages:

- preparation of raw materials;

- manufacture of water-alcohol extract of antlers;

- preparation of water-alcohol extracts from phyto-raw materials;

- concentration of extracts with complete extraction of alcohol;

- preparation of sugar-magnesium syrup;

- mixing;

- packaging and packaging of finished products.

Animal and phyto-raw materials are crushed on a grinder equipped with curly knives to a particle size of not more than 2 mm.

Preparation of water-alcohol extract pant

The estimated mass of crushed antlers is loaded into the hermetic container of the VKE-75 extractor, poured with a water-alcohol solution and insisted. The extraction is carried out 3 times with 50% ethyl alcohol at a temperature of 45 ° C. The 1st extraction is carried out for 2-24 hours with a ratio of antlers: extractant 1: 7 (weight: volume), at least 4 volumes of extractant are drained. For the 2nd extraction, 4 volumes of fresh extractant are loaded and the raw materials are extracted for 2-24 hours, at least 4 volumes of the extract are drained. For the 3rd extraction, 4 volumes of fresh extractant are loaded and the raw materials are extracted for 2-24 hours, at least 4 volumes of the extract are drained. The total amount of extract obtained is 12 volumes per 1 kg of raw material. The resulting extracts are combined and filtered on a cotton-gauze filter. The residual volume of 50% ethyl alcohol in the meal of the raw material after discharge, filtration and extraction is not more than 3 l per 1 kg of feedstock.

Preparation of water-alcohol extracts of phyto-raw materials

The estimated mass of crushed phyto-raw materials is loaded into a container for extraction, poured with a water-alcohol solution and insisted at a temperature of 40 ° C. Extraction is carried out with 70% ethyl alcohol for 4 days with a ratio of grass: extractant 1:10 (weight: volume). The resulting extract is filtered on a cotton-gauze filter. The residual volume of 70% ethyl alcohol in the meal of the raw material after discharge, filtration and extraction is not more than 2.5 liters per 1 kg of feedstock. This amount is considered technological loss.

Mixing and concentration of extracts with alcohol extraction

The prepared extracts of antlers and phyto-raw materials are mixed in the calculated ratio according to the rate of laying the raw materials in a rotary film evaporator. Concentration with the extraction of alcohol is carried out at a negative pressure and a temperature of no higher than 50 ° C to an extract volume of 6 l for the finished product with a total volume of 100 l. The residual content of ethyl alcohol in the concentrated extract should not exceed 1.5%. Then, in a rotary film evaporator, Polysorbate 80 is added in an amount of 1 liter for the finished product with a total volume of 100 liters. With constant stirring and normal pressure, the thick extract is dissolved in Polysorbate 80. Then, essential oil of clary sage in the amount of 0.2 l per 100 l of the finished product is added. The alcohol regenerated during distillation has a strength of about 75% and is used for further extraction.

Preparation of carbohydrate-magnesium syrup

The components are loaded into the digester at the rate of 40 kg of sugar and 6 kg of magnesium sulfate per 100 liters of finished products. Add 50 l of distilled water, bring to a boil with constant stirring, cool to 40-50 ° C and bring the total volume to 93 l.

Mixing concentrated extracts with syrup is carried out in a digester at a temperature not exceeding 40 ° C.

Packaging and packaging. The finished product is fed to the dosing and filling machine, packed in pre-prepared containers and sealed.

Examples of formulations made according to the described technology for balsam are given below.

Example 1. Panto-magnesium balm "Vitapant-1", wt.%:

alcohol-free extract concentrate from antlers 6.0 alcohol-free root extract safrole-shaped leuzea in concentrated form 0.5 magnesium sulfate 4.0 sugar 40,0 water rest

Example 2. Panto-magnesium balm "Vitapant-2", wt.%:

alcohol-free extract concentrate from antlers 5,0 alcohol-free root extract safrole-shaped leuzea in concentrated form 1,0 magnesium sulfate 6.0 clary sage essential oil 0.2 polysorbate 80 1,0 sugar 40,0 water rest

Example 3. Panto-magnesium balm "Vitapant-3", wt.%:

alcohol-free extract concentrate from antlers 3.0 alcohol free extract safrole-shaped leuzea in concentrated form 1,5 magnesium sulfate 7.0 polysorbate 80 0.5 honey 35.0 water rest

Example 4. Panto-magnesium balm "Vitapant-4", wt.%:

alcohol-free extract concentrate from antlers 6.0 alcohol free extract Aralia Manchurian in concentrated form 1,5 magnesium sulfate 5,0 polysorbate 80 fifteen clary sage essential oil 0.3 starch syrup 45.0 water rest

Example 5. Panto-magnesium balm "Vitapant-5", wt.%:

alcohol-free extract concentrate from antlers 4.0 alcohol-free root extract concentrated ginseng 0.5 magnesium sulfate 7.0 polysorbate 80 1,0 clary sage essential oil 0.25 food inulin from Jerusalem artichoke 25.0 shungite treated water rest

The proposed balm in liquid form contains concentrated extracts of animal and vegetable origin, obtained by extraction with ethyl alcohol and its subsequent removal from the product. In this regard, the alcohol-free pantap-magnesium balsam Vitapant, in comparison with the prototype, has a wider scope in medicine and the food industry, and the presence of magnesium sulfate provides higher immunomodulating properties.

Example 6. Data from the study of the effect of the VITAPANT panto-magnesium balsam on the functional activity of macrophages of peritoneal exudate

A technique was used to determine the functional activity of peritoneal macrophages using the NBT test, which reflects the level of redox metabolism of cells.

The production of reactive oxygen species (superoxide anion radical, singlet oxygen, hydrogen peroxide, etc.) accompanies activation of phagocytosis, increased bactericidal potentials of macrophages and reflects the level of redox metabolism of phagocytes. Since the process of reduction of nitro-blue tetrazolium to diformazan correlates with the formation of reactive oxygen species, the basis for assessing the functional state of macrophages is the reduction reaction of a colorless solution of nitro-blue tetrazolium reagent to dark blue diformosan.

Peritoneal cells were added to the wells of a 96-well plate (1 × 10 ⁶ cells / well) in RPMI medium with 5% cattle serum (cattle).

Cultivation was carried out for 2 hours in a CO ₂ incubator at 37 ° C. Then, the cells were washed with Hanks solution and 100 μl of nitro-blue tetrazolium at a concentration of 1 mg / ml and 50 μl of a 1% suspension of opsonized ram erythrocytes (EB) were added to each well. After incubation for 1 hour, the cells were washed with Hanks solution and fixed with 10% formalin solution. Next, the macrophage monolayer was washed with distilled water and dried. After that, 60 μl of 2M KOH and 70 μl of dimethyl sulfoxide were added to each well. The level of functional activity was evaluated by the change in optical density (OD) of the solution, which was measured at a wavelength of 620 nm on a Multiscan device. Indicators of functional activity of macrophages were expressed in units of OD × 100.

The experimental data were statistically processed using the Statgrapfics 5.0 software package. Student's t-test was chosen as a reliability criterion.

Table Data on oral administration of balsam solutions to rats to determine the functional activity of their macrophages No. gr. Description of drug administered to rats Indicators (average in the group) of the functional activity of macrophages (restoration of HCT) of rats after drug administration through 3 days 7 days 21 days one. Intact - 28.58 ± 13.96 48.01 ± 5.38 2. Water 26.86 ± 11.97 35.26 ± 5.55 55.50 ± 11.42 3. An aqueous solution of balsam according to example 4 (0.01 wt.%) 42.50 ± 23.26 53.69 ± 1.36 108.73 ± 7.60 four. An aqueous solution of balsam according to example 5 (0.01 wt.%) 41.87 ± 4.42 49.62 ± 2.79 91.87 ± 3.74 5. An aqueous solution of balsam in example 1 (0.01 wt.%), But without magnesium sulfate 33.87 ± 5.40 39.62 ± 3.59 69.87 ± 8.47 6. An aqueous solution of balsam in example 1 (0.01 wt.%), But without extract from antlers 31.21 ± 1.20 48.26 ± 1.77 71.78 ± 7.46

The above table shows indicators of the functional activity of resident peritoneal macrophages of Wistar rats 3 days, 7 days and 21 days after oral administration of a balm solution of various compositions.

Based on these data, we can draw a conclusion about the ability of the inventive VITAPANT panto-magnesium balsam to modulate the oxidation-reduction metabolism of rat peritoneal macrophages to a greater extent and enhance their functional activity in comparison with balsam solutions in which one of its main components is absent: either extract from antlers, or magnesium sulfate, which confirms the higher immunomodulating properties of the claimed product.

Claims (6)

1. Panto-magnesium balm, characterized in that it includes an alcohol-free concentrate of an extract from antlers, carbohydrates, magnesium sulfate, an alcohol-free plant extract of tonic action in a concentrated form, at least one type from the group: rhizomes with safflower-root roots, or roots ginseng, or the roots of Aralia Manchurian and water with the following components, wt.%:
alcohol-free extract concentrate from antlers 2.0-6.0 alcohol-free plant extract tonic effect in concentrated form 0.5-1.5 magnesium sulfate 4.0-7.0 carbohydrates 25.0-45.0 water rest 2. The balm according to claim 1, characterized in that it further comprises a vegetable essential oil that improves the taste of the product in an amount of 0.1-0.3 wt.%. 3. The balm according to claim 1, characterized in that it further comprises polysorbate 80 in an amount of 0.5-1.5 wt.%. 4. The balm according to claim 2, characterized in that as a vegetable essential oil, it contains essential oil of clary sage. 5. Balm according to claim 1, characterized in that it contains sugar or fructose, or honey, or starch syrup, or food inulin from Jerusalem artichoke as carbohydrates. 6. The balm according to claim 1, characterized in that as water it contains water treated with schungite.