RU2370151C1 - Bioactive food additive - Google Patents

Abstract

FIELD: biotechnology. ^ SUBSTANCE: invention concerns biotechnology, medical and food industry, area of food additives with bioactive properties. Bioactive additive (BAA) for food is based on raw plant material. Additive obtainment involves preparation of honey extractions of calendula, dandelion, peppermint and licorice in the form of infusions (decoctions), and pumpkin, chick-pea and milk thistle seeds germinated in serum. Each raw material, calendula, dandelion, peppermint and licorice, is independently extracted by honey at respective ratio of 1:5, and germinated pumpkin, chick-pea and milk thistle seeds are independently extracted by honey at 1:2 ratio. Further the honey extractions are mixed with lactulose where succinic acid was dissolved in advance. All components are taken in the following amount per 100 kg of product: honey extractions of calendula, dandelion, peppermint and licorice by 5.0 kg, honey extractions of germinated seeds by 10.0 kg, 49.7 kg of concentrated lactulose, 0.3 kg of succinic acid. ^ EFFECT: obtainment of bioactive additive with enhanced bioactivity, high bifidus factor, for immune system reinforcement, normal body function recovery and energy loss replenishment. ^ 4 tbl

Description

The invention relates to biotechnology, the medical and food industries in the field of food additives with biologically active properties.

The data accumulated in the field of nutritional science indicate that under the conditions of modern man’s life it is impossible to adequately provide the body’s needs with all the biologically active components necessary to support its vital activity through traditional nutrition. Deficiency of these nutrients and biologically active components in the diet leads to a decrease in the body's resistance to adverse environmental factors (maladaptation), the formation of immunodeficiency states, dysfunction of antioxidant defense systems, chronic diseases, an increase in the risk of developing common diseases, a decrease in the quality of life and the effectiveness of treatment measures .

To solve the above problems, therapeutic and prophylactic agents, biologically active food additives (BAA), related to the field of herbal medicine, have become widespread recently. Modern food technology offers the consumer a wide range of multicomponent compositions, balms, elixirs based on medicinal herbs for therapeutic and prophylactic purposes.

Known drug (medical elixir) containing valerian root, elecampane root, ginseng root, bee honey, nitroglycerin, and as an alcohol-containing component - water-alcohol liquid with a strength of 40% [RU 2000100143].

The soothing elixir “Harmony” is known, which contains Valerian root, motherwort, rosehip, mint leaf, hawthorn, calendula, St. John's wort, wild strawberry leaf, black cherry and common mountain ash, hawthorn inflorescences, chamomile, lemon balm, linden juice, lingonberry juice honeysuckle, blueberries, pear drink, sugar, citric acid [RU 96114105].

The healing properties of these elixirs make it possible to recommend them to a large group of the population for the treatment and prevention of many diseases. At the same time, their alcohol component limits the range of consumers of these elixirs.

There is evidence of the production of honey balms, which include herbal extracts.

Honey balm “Good night” soothes, relieves irritability, normalizes sleep. Balm composition: cones, hops, peony root, hawthorn fruits, viburnum fruits, mint leaf, cedar oil, mountain honey. Honey balm has a sedative-hypnotic effect, relieves mild signs of depression, irritability, improves overall health, and gently reduces blood pressure [1].

Stevia balm is a natural, ecological product obtained by the latest vitamin-saving technology, especially recommended for patients with diabetes mellitus. It contains extract of stevia, fruits of red mountain ash, maltose corn syrup, honey, tarragon, cinnamon, pink radiola root, citric acid. Manufacturer: JSC "Tablet" (Novosibirsk) [2].

The proposed honey balms have a wide spectrum of action, but do not affect the functioning of the gastrointestinal tract to a sufficient degree (for the prevention of dysbiosis), since they do not contain probiotic products.

As a prototype, a biologically active additive was selected, characterized in that it contains a concentrated aqueous extract of a mixture of plant materials from oregano grass, dandelion root, mountaineer grass, rosehips, bearberry leaf, common rowan fruits, fruits and flowers of hawthorn [RU 2287303 C1].

This product is positioned as a dietary supplement to improve the functional state of the gastrointestinal tract, it contains, in addition to the active substance, caffeine, ascorbic and citric acids, sodium benzoate, and a sweetener.

The drug does not have components that stimulate the development of intestinal microflora, and is also aimed at reducing appetite.

The objective of the invention is to develop a new effective and affordable means (biologically active food additives) for the prevention of diseases of the gastrointestinal tract, liver, to increase immunity and restore normal functions of the whole organism, with the additional property of restoring energy loss.

The technical result is an increase in the biological activity of the additive, the expansion of the spectrum of application.

This is achieved by the fact that a biologically active food supplement “Lactoflex” is proposed on the basis of honey extracts of calendula, dandelion, mint, licorice infusions (decoctions) in a ratio of 1: 5, honey extracts of pumpkin seeds, chickpeas, and milk thistle germinated on milk whey. : 2 in combination with lactulose and succinic acid.

Supplements are prepared by extracting plant materials with honey for 14 days in a dark, cool place, then honey extracts are mixed with lactulose, in which succinic acid is previously dissolved, per 100 kg of biologically active additives, 5.0 kg of honey extracts of dandelion, mint, licorice, calendula, 10 kg of honey extracts of germinated pumpkin seeds, chickpeas, milk thistle, 49.7 kg of lactulose concentrate, 0.3 kg of succinic acid.

The preparation technology consists in obtaining honey extracts of calendula, dandelion, mint, licorice, germinated on milk whey of pumpkin seeds, chickpeas, milk thistle, mixing extracts with lactulose concentrate in which succinic acid is previously dissolved.

For the preparation of extracts of medicinal plants (flower baskets and reed flowers of calendula, root and aerial parts of dandelion, peppermint leaves, roots and rhizomes of licorice) use selected plant materials. Aqueous extracts are preliminarily obtained by infusion in a tunic preheated for 15 minutes. Water extracts first insist on a water bath, and then at room temperature. Infusions are prepared from friable small plant materials: grass, flowers, leaves; decoctions are prepared from more dense raw materials: bark, root, rhizome according to table 1 [3].

Table 1 Modes of preparation of infusions and decoctions of herbs Type of extraction Extraction mode water bath, min room temperature, min Infusion fifteen 45 Decoction thirty 10 Infusion volume> 1000 ml 25 45 Broth with a volume of> 1000 ml 40 10

Then add honey in the ratio of the infusion of herbs (flowers): honey of light varieties - 1: 5. The mixture is infused for 14 days in a dark, cool place.

To prepare extracts of pumpkin, chickpea and milk thistle seeds germinated on milk serum, high-quality seeds are used, which are manually sorted, washed in a pink solution of potassium permanganate, then several times in running water, placed in one layer between two layers of linen or cotton cloth, soaked in warm spring water or water passed through a filter and left at + 22 ... + 24 ° С. The upper tissue is moistened with milk serum 2 times a day (morning and evening) until seedlings appear (piercing) (no more than 3 days). The use of whey reduces seed germination time and additionally enriches raw materials with nutrients. The length of the seedlings should be 1-2 mm. Seeds are sorted and washed. Germinated seeds are extracted with light varieties of honey in a ratio of 1: 2. The extraction process is 14 days in a cool dark place.

After exposure, honey extracts of plant materials are separated by filtration. Then, the required amount of succinic acid according to the formulation is dissolved in lactulose concentrate with slight heating (up to 60 ° C) and stirring. You can use any concentrate of food lactulose with a lactulose content of at least 34.6%. Next, honey extract and lactulose concentrate are mixed with succinic acid dissolved in it in accordance with the recipe (table 2) to a homogeneous mass.

table 2 Recipe for dietary supplement "Lactoflex" per 100 kg of product name of raw materials Units rev. amount Dandelion Honey Extract kg 5,0 Peppermint Honey Extract kg 5,0 Licorice Honey Extract kg 5,0 Calendula Honey Extract kg 5,0 Sprouted pumpkin seed honey extract kg 10.0 Milk Thistle Seed Extract kg 10.0 Sprouted Chickpea Honey Extract kg 10.0 succinic acid kg 0.3 Lactulose Concentrate "Lactusan" kg 49.7

The finished product is a light brown syrup with a pleasant sweet and sour taste and smell characteristic of its constituent components. According to organoleptic and physico-chemical indicators, the products included in the Lactoflex dietary supplement are well compatible, which does not cause unpleasant sensations when consumed. The basis of the composition is composed of selected and well-combined natural medicinal plants, which in complex application enhance their functional properties [4].

The action of the main active component - lactulose is enhanced by the introduction together with honey of biologically active substances from specially prepared plant materials (infusions and decoctions of herbs, germinated seeds). The presence of succinic acid gives the dietary supplement a pleasant sweet and sour taste.

The use of honey extracts of plant materials in the formulation of dietary supplements "Lactoflex" purposefully and scientifically sound.

Honey, being a high-calorie product (100 g of honey contains about 315 kcal), quickly replenishes the energy loss of the body, contains simple carbohydrates: glucose and fructose, so it is easily digestible. Honey is rich in vitamins, amino acids, various microelements, essential oils and other beneficial substances. The use of honey is recommended for functional disorders of various organs and systems, to relieve physical and mental fatigue. In addition, honey is a hygienic food product; bacteria are practically absent in it. All this makes it a promising component in the preparation of compositions for complex functional food additives.

Calendula (medicinal marigolds. Calendula Officinalis L.) contains carotenoids and flavonoids (carotene, lycopene, violaxanthin, citraxanthin, rubixanthin, flavoxanthin, flavochrome). There are also polysaccharides, polyphenols, organic acids (malic, ascorbic, salicylic). The pharmacological activity of calendula preparations is due to carotenoids and flavonoids. When used internally, the drug calendula exhibits anti-inflammatory activity, promotes the regeneration of the mucous membranes of the stomach and intestines, the healing of ulcers and erosion. The mechanism of the protective effect of the preparation of calendula in experimental gastric ulcers consists of a decrease in the aggressiveness of the gastric juice and an increase in the resistance of the gastric mucosa. Galenic flower forms of calendula have an antispasmodic effect, mainly on the stomach and intestines, relieve spasm of sphincters of the biliary tract, increase the secretory activity of the liver and the flow of bile into the duodenum. Infusions of calendula are used internally for peptic ulcer, erosive gastritis, colitis, enterocolitis, and are used as a choleretic agent.

Medicinal dandelion (Taraxacum officinale Web.) Contains hydroxycinnamic acids (chicory, kaftaric, chlorogenic, ferulic, coffee), polyfructosans (inulin, etc.), activates the function of the liver and pancreas, has a choleretic effect, stimulates the oxidation of cholesterol in the liver and its in the form of fatty acids; increases the detoxification activity of the liver. Dandelion bitterness (glycoside taraxanthin) - a means for stimulating appetite and choleretic.

Peppermint leaves (Mentha piperita L.) are used as an antispasmodic and choleretic agent. Mint enriches honey with flavanones (naringenin, hesperitin, eriodiktiol), essential oils (menthol), valerianic acid.

Licorice (Glycyrrhiza, Radices Liquiritiae) has a pronounced anti-inflammatory (corticosteroid-like) effect. Licorice saponins increase the body's adaptation. Glucuronic acid determines the high detoxification properties of the plant. Licorice is used to neutralize toxins, replenish energy resources, as well as for allergies and inflammatory processes, including arthritis and myositis.

The use of pumpkin seeds, chickpeas, and milk thistle in the awakened (germinated) state in the proposed dietary supplement involves the enrichment of the extractant (honey) with an additional amount of essential substances. When seeds are germinated, enzyme systems are actively formed, which, in turn, catalyze the production of vitamins and the mass of other biologically active substances, the content of which in seeds and its seedlings increases many times. Honey extracts of germinated seeds serve as a source of polyphenols, flavonoids, essential oils, a complex of vitamins, micro and macro elements. The extracts have antioxidant, immunostimulating, anti-inflammatory, bactericidal effects. Hepatoprotective properties are determined by the content of flavonoids and flavonolignans (silibin, silicristin, etc.), help stabilize the membrane structure of liver cells, increase metabolic activity, prevent cellular degeneration and fatty degeneration of the liver, and enhance its detoxification properties.

In addition to honey extracts of plant materials, dietary supplements include lactulose concentrate and biologically active organic acid, succinic.

Lactulose - a carbohydrate, a recognized bifidus factor, a classic means of influencing the metabolism of intestinal microflora, is used as a preventive and therapeutic agent in a number of diseases, especially in case of dysbiotic manifestations. It is widely known that lactulose is used for hyperammonemia, liver failure, the associated portal system of encephalopathy (PSE), salmonellosis, renal failure, colon adenoma, osteoporosis, to activate immunity suppressed by cirrhosis or infectious diseases [5].

Lactulose can be used as an energy source not only by bifidobacteria, but also by other beneficial microorganisms (Lac. Lactis, Str. Thermophilus, Lb. brevis, Lb. fermentum, Lb. acidophilus, Lb. casei).

In the proposed dietary supplement used food lactulose concentrate food "Lactusan" composition shown in table 3.

Table 3 The composition of the concentrate lactulose "Lactusan" The name of indicators Content Mass fraction,%: dry matter 58.6 lactulose 34.6 lactose 12,2 galactose 7.1 other sugars 1.3 total nitrogen 0.05 ash 0.63 calcium 0.21 sodium 0.4 magnesium 0,03 phosphorus 0,03 potassium 0.04

Biologically active succinic acid (E 363) is a powerful regulator of the body's defenses, plays an important role in metabolism, takes part in the biosynthesis of alkaloids, glycosides, amino acids and other biologically active compounds, serves as a link between the individual stages of the exchange of fats, proteins and carbohydrates.

Succinic acid in this composition enhances the preservative ability of honey, preventing the development of microflora introduced by plant components into the extract.

The composition of natural biologically active substances, obtained by combining honey extracts of calendula, dandelion, mint, licorice, germinated pumpkin seeds, chickpeas, milk thistle in combination with lactulose and succinic acid, makes Lactoflex food supplements a multifunctional product. The drug improves the function of the liver and biliary system, it is recommended to normalize the microflora of the large intestine, with gastritis, it facilitates the course of peptic ulcer disease, weakens toxic-allergic reactions in the body, and is used to prevent metabolic disorders (atherosclerosis, diabetes mellitus), and prevent immunodeficiency states. Due to the introduction of honey extracts of milk thistle and chickpea into the dietary supplement, the drug has a pronounced hepatoprotective effect and is recommended for toxic lesions (chemical, food, alcohol, drugs, etc.) and liver diseases of various origins.

BAA “Lactoflex” is a powerful bifidus factor and a source for replenishing energy losses. It is recommended for patients in the rehabilitation period to increase the immune status, as well as athletes with intense physical exertion. BAA "Lactoflex" is indispensable for chronic processes, as it helps not only to extend periods without exacerbations, but also contribute to the complete restoration of health.

The chemical composition of the Lactoflex dietary supplement is characterized by a high content of fructose and inulin, contains glucose, sucrose, dextrose, levulose, essential oils, aromatic compounds, tertripoids, including glycyrrhizic acid, phenylcarboxylic acids and their derivatives, coumarins, flavonoids, higher a alcohols, protein substances, glycosides, choline, asparagine, sterols, sterols, saponins, azulenes, polyphenols, organic acids and their derivatives, resins and resinous substances, a complex of vitamins, carotene and others arotinoids, antho- and leukoanthocyanins, nicotinic acid, bitterness, tannins, volatile, calcium, phosphorus, iron, manganese, boron, copper, nickel, molybdenum, cobalt, strontium salts.

The use of dietary supplements "Lactoflex" helps to improve metabolic processes in the body, has anti-atherosclerotic, antimicrobial, detoxifying and anti-inflammatory effects on the body, helps to normalize redox processes in the myocardium and liver, digestibility of iron, increase immunity and reduce the risk of developing many currently common diseases , reduces the load on the liver and kidneys, promotes the production of vitamins.

100 g of dietary supplement Lactoflex contains at least 18 g of lactulose, 0.3 g of succinic acid, 86 mg of inulin, 17.14 mg of polyphenols, 1.26 mg of flavonoids, 0.8 mg of vitamin E, 0.85 mg hydroxycinnamic acids.

The formulation of the proposed dietary supplement is developed, focusing on the recommended levels of consumption of food biologically active substances relative to the daily needs of the human body [6].

Table 4 presents data on the nutritional value of dietary supplements "Lactoflex".

Table 4 Nutrition value of 100 g dietary supplement "Lactoflex" Name of indicator Content Carbohydrates 57.0 g Fats 0.03 g Organic acids 0.40 g Squirrels 0.30 g The energy value 233.1 kcal

Recommended dosage: for adults - 1 dessert spoon (8 g) 2 times a day (morning and evening) with meals. Duration of admission is 6 weeks. The drug is taken in its pure form or by adding it to tea, water or another drink.

Information sources

1. [2008]. - Access mode:

[http://www.ortho.ru/agents/food/MedBal_DobrNight.htm].

2. [2008]. - Access mode:

[http://piokal.ru/index.php?productID=292].

3. [2008]. - Access mode:

[http://www.fito.nnov.ru/technology/technology01.phtml].

4. Pot T.G. Biologically active substances of medicinal plants and antibacterial food additives - inhibitors of harmful microflora of meat products / Sib. scientific research and project.-technologist. in-t reslave. S.-kh. products. - Novosibirsk, 2003 - 104 s. - p. 81-85.

5. Ryabtseva S.A. Lactulose technology / study guide. - M .: DeLi print, 2003 .-- p.24-26.

6. Recommended levels of food intake of food and biologically active substances MP 2.3.1.1915-04 / Moscow, 2004 - p.17, p.19, p.24, p.27, p.30, p.32.

Claims (1)

A biologically active food supplement based on plant materials, characterized in that the plant materials, including calendula, dandelion, peppermint, licorice in the form of infusions (decoctions), are each individually extracted with honey in a ratio of 1: 5, pumpkin seeds germinated in serum , chickpea seeds, milk thistle seeds are each individually extracted with honey in a ratio of 1: 2, then honey extracts are mixed with lactulose, in which succinic acid is pre-dissolved per 100 kg dietary supplement of 5.0 kg honey extract in marigold, dandelion, mint, peppermint, licorice, 10.0 kg of honey extracts germinated pumpkin seed, chickpea, Silybum, 49.7 kg of lactulose concentrate, 0.3 kg of succinic acid.