RU2342121C1 - Method of indirect endolymphatic antibioticotherapias in case of male domestic carnivorous urolithiasis - Google Patents

Abstract

FIELD: medicine; veterinary medicine.

SUBSTANCE: method includes lymphotropical antibiotic injection, and each time the drug which boosts lymph current is injected preliminary. Thus preliminary a mix of half daily antibiotic dose with 0.% Novocainum solution and blood autoserum is taken in a dose of 1 ml per 10 kg of domestic carnivorous weight. Then the mixture is injected by 1/2 of its parts in turn into a hypodermic scrotum fat on the right and on the left of its median seam once a day during 5 days.

EFFECT: increase of antibioticotherapia efficiency in case of uropoiesis organ desease of domestic carnivorous, decrease of antibiotics adverse influence by means of decrease of a daily drug dose, time of its application and its purposeful influence on the pathological center, increase of immunologic activity of patient's body, acceleration of his convalescence, reduction of the relapse probability and augmentation of the disease remission term.

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Description

The invention relates to veterinary medicine and can be used in the complex treatment of urolithiasis and urological syndrome in male domestic carnivores (males and cats), as well as the prevention of surgical infections during surgical interventions on the urinary organs of male domestic carnivores.

The fight against urinary tract infection is one of the most important priorities in the treatment of urolithiasis in domestic carnivores, since in most cases urolithiasis is complicated by pyelonephritis, which creates all the conditions for relapse.

With urolithiasis, chronic urinary retention in the urinary tract is observed, which leads to its secondary infection. Attention should be paid to this fact during catheterization, urethro- and cystotomies, which are often practiced in urolithiasis of dogs and cats, since in these operations, the infection in the residual urine can enter the bloodstream through the lumen of the venous vessels.

In order to prevent infection in the body of a sick animal, it is necessary to carry out antibiotic therapy for a long time. However, with traditional methods of administering antibiotics, a significant part of the administered drug is inactivated in the liver and accumulates in adipose tissue. This necessitates the use of high doses of antibiotics in order to create and maintain a therapeutic concentration of the drug in the focus of inflammation for a long time, which increases the risk of side effects, in particular sensitization of the body and the development of dysbiosis and candidiasis. In addition, there is a rapid elimination of the drug from the area of inflammation, which requires the introduction of antibiotics quite often. The methods of administering antibiotics commonly used in medical practice, including intravenous administration, as a rule, do not provide a therapeutic concentration of them in the lymphatic system and are not always effective.

There is a method of antibiotic therapy in the treatment of urolithiasis of domestic carnivores, consisting in their intramuscular or oral administration (M.I. Rabinovich. Chemotherapeutic agents: reference book: M. KolosS, 2004, p.3-97).

The disadvantage of this method is that the effectiveness of such therapy is far from desirable, since it requires the use of high doses of drugs, which leads to immunosuppression, toxic effects on the liver and kidneys, allergic diseases and the development of dysbacteriosis.

There is a method of treating chronic inflammatory diseases of the urinary tract in children, which consists in the fact that antibiotic therapy is carried out for 14-21 days by parallel courses of lymphotropic and intramuscular administration of an antibiotic, with 1/2 daily dose of the antibiotic administered daily lymphotropically, once, and the other 1/2 daily doses - twice intramuscularly, while lymphotropic administration is carried out subcutaneously, alternately to the right and left in the projection of the spermatic cord in boys, and in this projection, a follower is introduced each time but a drug that enhances lymph flow, a microcirculatory stimulator, and then the antibiotic itself (RF Patent No. 2199358, IPC A61P 13/00, 31/00, 2001, prototype).

The disadvantage of this method is the excessive consumption of antibiotics (1.5 daily doses), a long course of antibiotic therapy. In addition, urolithiasis is often accompanied by a decrease in the body's defenses as a result of uremia, which is aggravated by the immunosuppressive effect of antibiotics, which requires the introduction of various immunostimulants into the treatment regimen.

The aim of the invention is to increase the effectiveness of antibiotic therapy in diseases of the urinary organs of domestic carnivores, to reduce the adverse effect of antibiotics on the animal’s body by reducing the daily dose of the drug, the time of its use and its targeted effect on the pathological focus, increasing the immunological activity of the patient’s body, as well as accelerating its recovery, reduce the likelihood of relapse and increase the duration of disease remission

The technical result, in contrast to the prototype, is achieved due to the fact that the antibiotic is administered lymphotropically, and in this projection, a drug that enhances lymph flow, for example lidase, is pre-administered each time, and a mixture is prepared from a half daily dose of the antibiotic by 0.5% a solution of novocaine and 1 ml per 10 kg of the weight of males of domestic carnivorous autoserum, then the mixture is injected in 1/2 of its part into the subcutaneous tissue of the scrotum to the right and to the left of its middle suture alternately once a day for 5 days.

Example. The experiment was carried out on males of domestic carnivores. Moreover, all experimental animals with urolithiasis were divided into 4 groups: the first control of 15 males; the second - an experimental of 15 dogs; the third control of 15 cats, the fourth experimental of 15 cats. The first and third groups of male domestic carnivores (control groups), patients with urolithiasis, were treated using conventional antibiotic therapy, which consists in the intramuscular injection of cefazolin sodium salt in a 0.5% solution of novocaine in half daily dose twice a day.

The second and fourth groups of animals (15 males and 15 cats), patients with urolithiasis, were treated using the proposed method - indirect endolymphatic antibiotic therapy. For this, a mixture is prepared consisting of a half daily dose of an antibiotic, for example, cefazolin sodium salt dissolved in 0.5-4.0 ml of a 0.5% novocaine solution with 1 ml per 10 kg of weight of male carnivorous autoserves. Then, 5-16 units of lidase dissolved in 0.5-1.0 ml of 0.5% novocaine solution are stimulated to the subcutaneous tissue of the scrotum to the right and left of its midline suture in order to stimulate lymph flow, then through the same needle in the same projections introduce the mixture prepared above, alternately 1/2 of the mixture into the subcutaneous tissue of the scrotum to the right and to the left of its middle suture once a day.

During treatment, needles with a minimum diameter were used. The resulting mixture was introduced very slowly, with minimal pressure on the plunger of the syringe. The course of treatment was carried out once a day for 10 days, but at the same time, males of domestic carnivores were monitored daily until complete recovery, as well as a general clinical blood test was performed before treatment, on the 3rd, 5th, 7th and 10th days.

The choice of antibiotic is determined by the sensitivity of microorganisms to it, the absence of its local irritating effect and its tolerance to animals (the use of cephalosporins, lincomycin and ampicillin is most justified; gentamicin is not recommended because of its immunosuppressive and nephrotoxic effects).

The research results are shown in tables 1 and 2.

Table 1 Comparative efficacy of antibiotic therapy for urolithiasis in dogs (M ± m) No. Indicators Norm The generally accepted method of antibiotic therapy (administration of cefazolin sodium salt in a 0.5% solution of novocaine in a half daily dose intramuscularly 2 times a day) Experience 1 (control) The inventive method of indirect endolymphatic antibiotic therapy Experience 2 The initial state 3 days 5 days 7 days 10 days The initial state 3 days 5 days 7 days 10 days one Red blood cells, mln / μl 5.5-8.0 4.32 ± 0.92 4.01 ± 0.35 4.33 ± 2.11 4.51 ± 0.62 4.40 ± 0.18 4.41 ± 0.34 4.72 ± 2.87 5.62 ± 0.46 5.64 ± 0.42 5.58 ± 0.24 2 Hemoglobin, g / l 110-180 96.23 ± 0.53 97.14 ± 3.18 99.04 ± 0.31 101.12 ± 0.35 101.04 ± 0.58 91.47 ± 0.82 99.14 ± 0.37 111.99 ± 0.13 110.93 ± 0.22 113.65 ± 0.37 3 White blood cells, thousand / μl 8.0-18.0 21.08 ± 1.24 20.98 ± 0.35 19.89 ± 0.93 18.68 ± 0.61 17.05 ± 0.14 21.43 ± 0.64 19.14 ± 0.45 16.7 ± 0.57 16.04 ± 0.52 15.93 ± 0.32 four ESR, mm / 15 min 0-0.4 0.57 ± 1.21 0.49 ± 0.76 0.46 ± 1.73 0.40 ± 0.51 0.30 ± 0.73 0.60 ± 1.26 0.54 ± 0.47 0.26 ± 0.68 0.27 ± 0.61 0.24 ± 0.11 5 Phagocytic activity,% 40-80 22.11 ± 0.93 27.34 ± 0.63 29.05 ± 1.42 28.43 ± 0.58 27.43 ± 0.52 21.34 ± 0.34 32.56 ± 1.24 44.87 ± 0.58 44.51 ± 0.57 43.62 ± 0.51 6 Phagocytic index 4-9 1.26 ± 0.75 2.57 ± 1.42 3.04 ± 0.52 3.11 ± 0.69 3.41 ± 0.51 1.57 ± 0.16 3.39 ± 0.67 5.03 ± 0.52 5.00 ± 0.13 4.43 ± 0.11 7 Clinical improvement in the number of patients,% one hundred 13.33 33.33 40,0 46.67 40.00 86.66 86.66 86.66 8 The number of relapses in the group for the year,% 0 40,0 26.67

table 2 Comparative effectiveness of antibiotic treatment methods for urolithiasis in cats (M ± m) No. Indicators Norm The generally accepted method of antibiotic therapy (administration of cefazolin sodium salt in a 0.5% solution of novocaine in a half daily dose intramuscularly 2 times a day) Experience 3 (control) The inventive method of indirect endolymphatic antibiotic therapy Experience 4 The initial state 3 days 5 days 7 days 10 days The initial state 3 days 5 days 7 days 10 days one Red blood cells, mln / μl 6.6-9.4 4.96 ± 1.64 4.98 ± 0.76 5.01 ± 1.85 5.00 ± 0.71 5.12 ± 0.49 4.38 ± 0.71 5.49 ± 1.39 6.67 ± 0.91 6.59 ± 0.62 6.71 ± 0.79 2 Hemoglobin, g / l 100-140 96.01 ± 0.39 97.42 ± 1.28 96.24 ± 0.81 98.54 ± 0.71 98.11 ± 0.28 93.61 ± 0.22 98.66 ± 0.11 103.20 ± 2.59 104.05 ± 0.93 105.43 ± 0.16 3 White blood cells, thousand / μl 10.0-20.0 23.98 ± 0.77 22.66 ± 0, b5 21.99 ± 0.53 13.04 ± 0.52 11.69 + 0.87 24.04 ± 0.81 21.15 ± 0.22 19.03 ± 0.62 19.11 ± 0.36 18.99 ± 0.11 four ESR, mm / 15 min 0-0.2 0.47 ± 3.13 0.32 ± 0.21 0.31 ± 0.91 0.30 ± 0.83 0.20 ± 0.41 0.50 ± 2.18 0.33 ± 0.28 0.19 ± 0.11 0.17 ± 0.45 0.18 ± 0.33 5 Phagocytic activity,% 40-80 27.14 ± 0.83 29.01 ± 0.22 30.15 ± 1.47 31.97 + 0.14 34.14 ± 2.41 24.52 ± 0.93 38.67 ± 0.48 45.23 ± 0.11 42.18 ± 0.23 43.98 ± 0.99 6 Phagocytic index 4-9 1.42 ± 0.35 1.39 ± 0.67 1.87 ± 0.14 2.41 ± 0.73 2.56 ± 0.51 1.36 ± 0.72 3.49 ± 0.55 5.41 ± 0.61 5.40 ± 0.13 5.24 ± 0.62 7 Clinical improvement in the number of patients,% one hundred 26.67 40.00 66.67 46.67 80.00 80.00 80.00 8 The number of relapses in the group for the year,% 0 60.00 20.00

1/2 of its part was injected alternately into the subcutaneous tissue of the scrotum to the right and left of its middle suture once a day, the following conclusions were drawn:

When analyzing tables 1 and 2, it can be seen that already on the 3rd day of antibiotic therapy by the claimed method, an improvement in all hematological parameters in males (experiment 2) and cats (experiment 4) was noted compared with their initial state. At the same time, the content of red blood cells after 3 days of treatment by the claimed method increased 1.25 times in cats (experiment 4) and 1.07 times in dogs (experiment 2); hemoglobin content of 1.05 and 1.08 times, respectively; phagocytic activity 1.58 and 1.53 times, respectively; phagocytic index 2.57 and 2.16 times, respectively; in addition, there was a decrease in the number of white blood cells in the blood by 1.14 times in cats (experiment 4) and by 1.12 times in dogs (experiment 2); ESR in 1.52 and 1.11 times, respectively. In addition, after 3 days of treatment, significant clinical improvement was observed in 46.67% of cats (experiment 4) and 40.0% of dogs (experiment 2).

After 3 days of treatment with the generally accepted method of antibiotic therapy (experiments 1.3, control), improvement of hematological parameters in males and cats was not observed, clinical improvement was noted in 13.33% of males.

After 5 days of indirect endolymphatic antibiotic therapy (by the claimed method), all hematological parameters were completely normalized compared to the initial state of male carnivores with urolithiasis, expressed in a 1.52-fold increase in red blood cells in cats (experiment 4) and 1.27 times in males (experiment 2) (100% of the norm); hemoglobin content of 1.10 and 1.22 times (100% of normal), respectively; phagocytic activity in 1.84 and 2.1 times (100% of normal), respectively; phagocytic index 3.98 and 3.2 times (100% of normal), respectively; as well as a decrease in the content of leukocytes by 1.26 times in cats (experiment 4) and 1.28 times in dogs (experiment 2), respectively (100% of the norm) and a decrease in ESR of 2.63 in cats (experiment 4) and 2.31 times in males (experiment 2), respectively (100% of the norm). In addition, there was a complete optimization of the clinical condition, manifested by the restoration of urination, a good appetite and an alert state in 80.0% of males and 86.66% of males.

After 5 days of antibiotic therapy in the generally accepted way (experiments 1.3, control), a slight increase in the erythrocyte content was 1.08 times in cats (experiment 3) and 1.002 times in dogs (experiment 1); an increase in hemoglobin content of 1.002 and 1.029 times, respectively; an increase in phagocytic activity of 1.11 and 1.32 times, respectively; an increase in the phagocytic index of 1.32 and 2.41 times, respectively; a decrease in white blood cell count of 1.09 and 1.06 times, respectively; a decrease in ESR by 1.52 and 1.24 times, respectively, compared with the initial state, which indicates a slight improvement in the condition of males of domestic carnivores, but still does not correspond to the optimal indicators. Clinical improvement was observed in 26.67% of males and in 33.33% of males (Tables 1, 2).

On the 7th and 10th day with indirect endolymphatic antibiotic therapy (the claimed method), there were no significant changes in the hematological parameters and the clinical condition of domestic carnivores.

In the usual method of antibiotic therapy (intramuscular administration of cefazolin sodium salt in a 0.5% solution of novocaine in half daily dose 2 times a day) on the 7th and 10th days, some improvement in the hematological parameters and clinical condition of patients continued, however, even with Such a long treatment time, these indicators are not in all cases optimized. So, by the 10th day of treatment, the content of red blood cells increased 1.03 times in cats (experiment 3), which corresponds to 77.58% of the norm, and 1.02 times in dogs (experiment 1) (80% of the norm) ; the hemoglobin content increased 1.02 times in cats (98.11% of the norm) and 1.059 times in dogs (91.85% of the norm); the leukocyte count decreased 2.05 times in cats (100% of the norm) and 1.24 times in dogs (94.7% of the norm); ESR slowed down 2.35 times in cats (100% of the norm) and 1.9 times in dogs (100% of the norm); phagocytic activity increased 1.26 times in cats (85.35% of the norm) and 1.24 times in males (68.58% of the norm); the phagocytic index increased 1.80 times in cats (64.0% of the norm) and 2.71 times in males (85.25% of the norm). There was also a further improvement in the clinical condition of males of domestic carnivores and by the 7th day of therapy, clinical improvement was observed in 40% of cats and 40% of males, and by 10th day - in 66.67% of cats and in 46.67% males.

When comparing a 5-day course of treatment of males of domestic carnivores (experiments 2, 4) with the claimed method and a 10-day course of treatment in the generally accepted way (experiments 1, 3), an increase in the content of red blood cells by 1.3 times in cats and by 1.28 times in males, hemoglobin by 1.05 times in males and 1.11 times in males, phagocytic index 2.11 times in willow cats 1.48 times in males, ESR deceleration by 1.05 times in cats and 1 , 15 times in males and an improvement in the overall clinical condition by 1.20 times in males and 1.86 times in males.

Therefore, the best results (Tables 1, 2) were obtained when treating males and cats of domestic carnivores with urolithiasis, the claimed method: first, in the subcutaneous tissue of the scrotum to the right and to the left of its middle suture, 5-16 units of lidase dissolved in 0.5-1.0 ml of a 0.5% solution of novocaine, then through the same needle in the same projection a mixture is administered consisting of a half daily dose of an antibiotic, for example, cefazolin sodium salt dissolved in 0.5-1.0% novocaine , with the addition of 1 ml per 10 kg of weight of males of domestic carnivores x autosyvorotki blood, once a day for 5 days.

After the treatment, we conducted regular clinical examinations and examinations of all animals participating in the experiment for 12 months. During this time, relapses of urolithiasis and bacterial infections of the urinary organs were observed in 20% of cats and 26.67% of males with the claimed method of indirect endolymphatic therapy. With the generally accepted method of antibiotic therapy, these indicators were significantly higher and amounted to 60% in cats and 40% in dogs.

Based on the data obtained, it can be concluded that the claimed method of indirect endolymphatic therapy for urolithiasis in male carnivores allows to reduce the treatment time by at least 5 days, reduce the daily dose and the frequency of administration of antibiotics by half, and as a result reduce their toxic and immunosuppressive effects on the body, increase the overall immunological status of males of domestic carnivores and as a result increase the percentage of recovered animals and further reduce the likelihood of recurrence of Ia.

According to research, when a mixture of an antibiotic and blood serum is injected into the subcutaneous tissue of the scrotum, the drug quickly reaches and selectively accumulates in the lymph nodes, and from there it is retrograde distributed into the pelvic organs, creating an optimal concentration in the interstitium. At the same time, lidase causes a narrowing of the lymphatic vessels, an increase in the frequency and amplitude of pulsation of the lymphangions and an increase in lymphatic drainage, promoting the absorption of the drug not into the bloodstream, but into the lymphatic channel. Blood serum has an immunostimulating effect and also promotes the penetration of the solution into the lymphatic capillaries. strengthens blood and lymph flow, increases the rate of excretion of metabolic products and reduces congestion in the pathological focus. In addition, autoserum is an absolutely harmless drug that does not have side effects, and the likelihood of a deficiency of this component is completely eliminated.

The method can be easily implemented in the veterinary practice of clinics specializing in the treatment of male carnivores.

Claims (1)

A method of indirect endolymphatic antibiotic therapy for urolithiasis in males of domestic carnivores, comprising administering the antibiotic lymphotropically, each time preliminarily administering a drug that enhances lymph flow, characterized in that a mixture is prepared from a half daily dose of the antibiotic in a 0.5% solution of novocaine and autoserum blood, which is taken at a dose of 1 ml per 10 kg of the mass of domestic carnivores, then the mixture is injected in 1/2 of its part alternately into the subcutaneous tissue of the scrotum to the right and left of its middle suture once a duck for 5 days.