RU2392928C2 - Method of endometritis treatment and prevention in cows - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: invention relates to veterinary science. The method involves intrauterine introduction of medicinal preparations. A medicinal mixture of 2% Chlorophyllipt solution in oil and vitamin preparation Trivit in oil in the ratio 1:1 respectively is prepared. Then the mixture in amount 10-20 ml per a head is introduced intrauterine 2-3 times every 48 hours.

EFFECT: method is highly efficient, allows improving impregnation capacity of animals.

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Description

The invention relates to veterinary medicine, in particular to drugs for the treatment and prevention of endometritis.

Chronic endometritis is an inflammation of the predominantly uterine mucosa. Depending on the nature of the inflammation, endometritis is divided into catarrhal, purulent-catarrhal and purulent. It is believed that the main form of manifestation of chronic endometritis in cows is purulent-catarrhal endometritis.

For the treatment and prevention of postpartum diseases in cattle, antimicrobial drugs are used, including antibiotics, sulfonamide, nitrofuran agents. But not all of them give the expected effect. This is primarily due to the rapid development of resistance of the uterine microflora to the antimicrobial agents that make up their composition. Therefore, periodic replacement of drugs is necessary as their therapeutic effectiveness decreases. In this regard, there is a need to develop new methods for the treatment and prevention of endometritis in cows through the use of new effective drugs or mixtures of various drugs.

A known method of treatment by using the intrauterine antimicrobial drug Furacoll in the form of an emulsion containing 10% collagen paste and 0.1% furatsilina. The emulsion is administered to cows with an acute form of endometritis at a dose of 100 ml / goal. 3-4 times with an interval of 48 hours. The therapeutic efficacy of the drug is 81% (Sein O.B., Furman Yu.V., Besedin M.V., Bugorsky R.G. Application of the drug Furacoll for the treatment of endometritis of cows. // Abstract report of the coordination meeting. / Results and the prospects of scientific research on the problems of animal pathology and the development of therapies and methods of therapy and prevention (Voronezh, 1995, p.240-242).

The disadvantage of the drug is the weak ability of collagen to penetrate the uterine mucosa and lack of therapeutic efficacy.

A known method for the prevention of postpartum endometritis in cows using the intrauterine preparation Zhiroform-BM. The drug is a polymer suspension with a copper sulfate content of 20-25% and brilliant green 0.05-0.1%.

To prevent postpartum endometritis, the drug is administered after surgical separation of the placenta intrauterine dose of 100 ml three times with an interval of 48 hours. The preventive effect of administering the drug to cows for the prevented disease of postpartum endometritis is 87.5%. (Gurchenko A.M., Limarenko A.A. Application of the Zhiroform-BM preparation for the treatment and prevention of endometritis in cows. // Abstracts of the coordination meeting. / Results and prospects of scientific research on animal pathology and the development of treatment methods and methods and prevention, Voronezh, 1995, p. 220-222).

The disadvantage of this method is the significant total volume of the administered drug and the increased frequency of its administration. In addition, it is known that in single-component preparations containing one drug, bacterial resistance develops faster.

The closest in technical essence to the claimed invention is a method of treating endometritis, where an intrauterine preparation - iodismuthsulfamide is used, containing the following components per 1 kg of emulsion: iodismuthsulfamide - 50 g, sunflower oil - 390 ml, trivit - 10 ml, emulsifier 1- 40 g, distilled water - 510 ml (Klenova I.F. et al. Veterinary preparations in Russia. Reference book. - Moscow: Selkhozizdat, 2004, p. 481, prototype).

The disadvantage of this method is the pronounced irritating effect of the drug on the mucous membrane of the uterus due to the high content of free iodine and, as a result, weak penetrating ability.

The technical result is an increase in the effectiveness of treatment and prevention of chronic endometritis in cows.

The technical result, in contrast to the prototype, is achieved by the fact that the method of treating endometritis in cows involves the intrauterine administration of drugs, trivit, moreover, the drug mixture is preliminarily prepared from a 2% oil solution of chlorophyllipt and vitamin Trivit oil preparation in a ratio of 1: 1, respectively, then the mixture in quantity 10-20 ml per head is administered intrauterine 2-3 times with an interval of 48 hours until the cows recover completely.

We have proposed a method for the treatment and prevention of endometritis in cows, in which a medicinal mixture of a 2% oil solution of chlorophyllipt and the vitamin Trivit oil preparation is used in a ratio of 1: 1, respectively, as an intrauterine preparation.

For the treatment and prevention of endometritis in cows, for the first time in veterinary practice we used a 2% oily solution of chlorophyllipt and for the first time used its intrauterine administration, no similar data were found in literature and patent sources.

A medicinal mixture of a 2% oil solution of chlorophyllipt and the oil vitamin drug Trivit in a ratio of 1: 1, respectively, was proposed as an intrauterine preparation because a larger dilution of the drug does not have a bacteriostatic and bactericidal effect on microorganisms isolated in cows from endometritis from cervical mucus.

The proposed combination of a 2% oily solution of chlorophyllipt and the oil vitamin drug Trivit in a ratio of 1: 1, respectively, differs from the prototype in that the extract from eucalyptus leaves (2% oily solution of chlorophyllipt), the active substance of which is chlorophyll, acts as an antimicrobial preparation.

We conducted tests in the SEC "Shilinsky" of the Sukhobuzim district of the Krasnoyarsk Territory on cows of black-motley breed, patients with chronic purulent-catarrhal endometritis with a livestock of 12 animals in each group. The treatment method adopted in the SEC "Shilinsky" was taken for control. The experimental design is presented in table 1.

Table 1 Experience outline No. Group Treatment regimens Dose of administration, ml / head one 1 - control Mixture: tetracycline hydrochloride - 5 g, ethacridine lactate - 0.15 g, ichthyol - 7 g, tincture of white hellebore - 4 ml, distilled water to 150 ml 150 2 2 - experienced Mixture: 2% oily solution of chlorophyllipt + trivit in a ratio of 1: 1 10 3 3 - experienced Mixture: 2% oily solution of chlorophyllipt + trivit in a ratio of 1: 1 twenty four 4 - experienced Mixture: 2% oily solution of chlorophyllipt + trivit in a ratio of 1: 1 thirty

The drug mixture for animals of the second, third and fourth experimental groups was prepared from pharmacopeia preparations by mixing in a sterile syringe of the company BU KWANG MEDICAL INC 2% oil solution of chlorophyllipt and trivit immediately before administration in a ratio of 1: 1, respectively.

The drug mixture used to treat animals of the first group was prepared immediately before administration in a glass flask, previously distilled water was sterilized by boiling. Then, ethacredine lactate was dissolved in hot distilled water, and after settling the solution to room temperature, the remaining components of the mixture were introduced (ichthyol, tetracycline hydrochloride, tincture of white hellebore).

The drug was administered intrauterine with an interval of 48 hours until complete recovery.

The condition of the genital organs of cows was evaluated by the dynamics of clinical signs.

In the animals of the control group, the solution was administered intrauterine using a Janet syringe connected by a rubber tube. The animals of the experimental groups were injected with a medical syringe, designed for 50 ml, connected to a rubber tube. The free end of the rubber tube was inserted through the cervix into the uterine cavity using a hand inserted into the vagina, and then the drug was administered. All solutions were heated to 37 ° C before administration.

The interval of drug administration in all groups was 48 hours until complete recovery.

Fifteen minutes before the administration of the drug mixture, all animals underwent rectal massage of the uterus to activate motor function. Massage, in turn, causes irritation of the nerve receptors of the cervix and uterus, leads to reflex secretion of oxytocin, which activates the motor processes of these organs, increases their tone, hyperemia and enhances local metabolic processes.

table 2 Recovery time and cost of treatment for animals with endometritis Indicator Animal number Group average one 2 3 four 5 6 7 8 9 10 eleven 12 1 group - control Duration of treatment, days 8 10 10 10 8 10 10 8 10 10 10 10 9.5 The number of injections of the drug, times / head 3 four four four 3 four four 3 four four four four 3.92 The cost of one introduction, rubles / goal. 50.00 The cost of the course of treatment, rubles / head 150.0 200,0 200,0 200,0 150.0 200,0 200,0 150.0 200,0 200,0 200,0 200,0 187.5 2 group - experienced Duration of treatment, days 6 8 10 8 8 6 8 10 6 8 6 6 7.5 The number of injections of the drug, times / head 2 3 four 3 3 2 3 four 2 3 2 2 2.75 The cost of one introduction, rubles / goal. 24.15 The cost of the course of treatment, rubles / head 48.30 72.45 96.60 72.45 72.45 48.30 72.45 96.60 48.30 72.45 48.30 48.30 66.41 3 group - experienced Duration of treatment, days 6 8 6 8 8 6 8 8 6 8 6 6 7.0 The number of injections of the drug, times / head 2 3 2 3 3 2 3 3 2 3 2 2 2.5 The cost of one introduction, rubles / goal. 48.30

The cost of the course of treatment, rubles / head 96.6 141.1 96.6 141.1 141.1 96.6 141.1 141.1 96.6 141.1 96.6 96.6 118.85 4 group - experienced Duration treatment days 6 8 8 6 6 6 6 6 8 8 6 8 6.8 The number of injections of the drug, times / head 2 3 3 2 2 2 2 2 3 3 2 3 2.42 The cost of one introduction, rubles / goal. 72.45 The cost of the course of treatment, rubles / head 144.90 217.35 217.35 144.90 144.90 144.90 144.90 144.1 217.35 217.35 144.90 217.35 175.09

An analysis of the data in Table 2 showed that the greatest duration of treatment was observed in the first group, here in 9 animals out of twelve, complete recovery was observed on the tenth day after the start of treatment and the average duration in the group was 9.5 days; whereas in the second experimental group for 10 days, treatment was continued in two animals; and in the third and fourth groups, the maximum treatment period was 8 days - 50% and 41.67% of the animals, respectively.

In the second, third and fourth experimental groups, the minimum treatment duration was 6 days, there were five such animals in the second group (41.67%), six in the third (50.00%), and seven in the fourth (58.33%). In the control group, the minimum treatment period was 8 days in three animals (25.00%).

In the control group of animals, recovery occurred in 9 animals after 4 injections of the drug mixture and in 3 after the third injection. In the second experimental group, the drug mixture was injected from two to four times, so four times the drug was observed in two (16.66%) animals. The largest number of recovered animals in the second and third injections was observed in the third and fourth groups.

Due to the varying duration of treatment, the number of drug administrations was different. The smallest number of injections of the drug mixture was noted in the third and fourth experimental groups of animals that received a mixture of 2% oily solution of chlorophyllipt and trivit (the mixture was prepared in a ratio of 1: 1) at a dose of 20 and 30 ml per head. Here, the average rate of injections of the drug mixture was 2.50 and 2.42 times, respectively, which is lower than in the control group by 36.22 and 38.27%. In the second experimental group, where the drug mixture was administered at a dose of 10 ml / goal, the amount of drug administration averaged 2.83 times, which is lower than in the control, by 27.81% and slightly higher than in the third and fourth groups , by 11.66 and 14.49%, respectively.

The cost of one injection of the drug mixture in the first group of animals was 50 rubles per goal, and the cost of a full course of treatment for one animal in the group average was 187.5 rubles. The cost of one administration of the drug was the lowest in the second group and amounted to 24.15 rubles / goal, and in the third group this figure was 48.3 rubles. However, due to a significant reduction in the duration of treatment in the group of animals treated with the drug mixture of 2% oil solution of chlorophyllipt and vitamin Trivit oil in a ratio of 1: 1 in a dose of 20 ml (3rd experimental group), the cost of the course of treatment on average for the group was lower than in the control and the fourth group, by 68.65 and 56.64 rubles. respectively, and higher than the second experimental group, 52.44 rubles.

From the foregoing, it follows that when using a medicinal mixture of a 2% oil solution of chlorophyllipt and oil vitamin drug Trivit in a ratio of 1: 1 in doses of 10, 20 and 30 ml, respectively, both the duration and the cost of the course of treatment for cows with endometritis are reduced. The duration of treatment in the second, third and fourth groups was lower than in the control by 21.05, 26.32 and 28.42%, respectively, and the cost of the course of treatment was 64.58, 36.61 and 6.62%. The optimal doses for administering the drug mixture of a 2% oil solution of chlorophyllipt and the oil vitamin drug Trivit in a ratio of 1: 1, respectively, are 10 and 20 ml / per head intrauterine 2-3 times with an interval of 48 hours. With the introduction of the drug mixture of a 2% oil solution of chlorophyllipt and the vitamin Trivit oil preparation in a ratio of 1: 1, respectively, in an amount of 30 ml / goal. in 4 groups of animals it was observed that the duration of treatment was slightly less than 2.94% compared with the third group, and the cost of the course of treatment increased sharply and increased by an average of 32.12% in the group.

Table 3 The effectiveness of the use of the drug mixture for endometritis in cows Indicators 1 group control 2 group experienced 3 group experienced 4 group experienced The number of treated cows, goals 12 12 12 12 The number of recovered cows, goals 12 12 12 12 Duration of treatment, days 9.5 7.5 7.0 6.8 The number of pregnant cows from cured heads 8 10 12 12 % Pregnant cows from cured 66.67 83.33 one hundred one hundred

The analysis of table 3 showed that in the third and fourth experimental groups, pregnancy occurred in 100% of the cows after the first insemination, in the first and second groups the percentage of pregnant cows from the cured was 66.67 and 83.33%, respectively.

Table 4 Blood counts of animals after treatment Indicator Group 1 control 2 experienced 3 experienced 4 experienced Red blood cells, 10 ¹² / L 6.25 6.64 6.40 6.50 White blood cells, 10 ⁹ / L 8.45 8.86 9.12 8.15 Hemoglobin, g / l 100.15 98.05 109.03 97.16 Total protein, g / l 63.40 68.80 78.50 69.80

Analysis of the data in table 4 showed that the blood of the cows of the control and experimental groups by the content of red blood cells and white blood cells did not have significant deviations. In the third group of animals, an increase in hemoglobin content relative to the control group by 8.87%, relative to the second and fourth groups, by 10.07 and 10.89% was observed. The content of total protein in blood serum in the second and fourth groups of animals was higher than in the control, by 8.52 and 10.09%. The blood serum of the third group of animals in this indicator exceeded the control group by 23.82%, the second and fourth groups by 12.36 and 11.08%. However, it should be noted that the blood indices of the animals of the control group and the experimental groups corresponded to physiological norms and did not have significant differences.

Thus, we can conclude that the therapeutic efficacy of the drug mixture of a 2% oil solution of chlorophyllipt and the oil vitamin drug Trivit in a ratio of 1: 1, respectively, in doses of 10 and 20 ml per head with 2-3 times a day with an interval of 48 hours at a temperature of the mixture 37 ° С positively affects the degree of recovery of sick cows, increases the percentage of fertilization of animals to 83.33-100%, which helps to reduce the cost of maintaining animals and increase the productive qualities of animals (getting young and dairy oduktivnosti).

Claims (1)

A method for the treatment and prevention of endometritis in cows, including the intrauterine administration of drugs, trivitis, characterized in that the drug mixture is preliminarily prepared from a 2% oil solution of chlorophyllipt and vitamin Trivit oil preparation in a ratio of 1: 1, respectively, then the mixture in an amount of 10- 20 ml per head is administered intrauterine 2-3 times with an interval of 48 hours.