RU2266124C1 - Method for treating uterine subinvolution in cows - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: the present innovation deals with applying 7%-ichthyol against 0.85% sodium chloride solution and denatured emulsified placenta (DEP), the latter to be introduced subcutaneously at the dosage of 20 ml on the 1^st, 5^th, and 9^th d of therapeutic course. Sterile 7%-ichthyol should be injected thrice subcutaneously being heated up to animal's body temperature on the 1^st, 3d and 5^th d at the dosages of 25, 30 and 35 ml.

EFFECT: higher efficiency of therapy.

4 ex, 4 tbl

Description

The invention relates to the field of veterinary medicine, in particular to methods for treating uterine subinvolution in cows.

Uterine subinvolution is a disease characterized by a slowdown in the development of the uterus after childbirth to a state inherent in this organ in non-pregnant animals. Its particular danger to subsequent reproductive function in cows is that purulent or purulent-catarrhal endometritis and functional disorders of the ovaries very often develop against its background.

There are three forms of manifestation of uterine subinvolution: acute develops in the first days after childbirth and proceeds in a severe form, subacute proceeds in a mild form and is detected, usually two to three weeks after childbirth, chronic is diagnosed one month or more after childbirth.

In the acute (severe) form of the pathological process, by 6-7 days, the lochia acquire a brown-brown or dirty-brown color, a watery consistency, an admixture of gray-brown flakes of a crumbly mass, an unpleasant putrefactive odor. Attempts are made in the cow, the tail root is raised, the animal assumes a urination position, general depression, a decrease in appetite and milk productivity are noted. In individual cows, body temperature rises, the scar reduction becomes weaker and the scar decreases. During rectal examination, the uterus is revealed deep in the abdominal cavity, is not covered by the hand, is atonic, fluctuates, its walls are flabby, without pronounced folding. Clinical signs and anatomical state of the genitals indicate a sharp violation of the contractile function and retraction of the uterine muscle fibers, the accumulation of decomposing lochia in the cavity and intoxication of the animal’s body, i.e. a severe form of subinvolution of the uterus often proceeds as a postpartum sapremia (the appearance of putrefactive bacteria in the blood).

The subacute (mild) form of the course of uterine subinvolution is diagnosed from 15 to 30 days after calving and is characterized by a prolonged discharge (up to 25-30 days after birth) of red-brown or dark brown, thick, greasy consistency of lochia, usually after a night's rest or massage of the uterus through the rectum. The uterus is usually enlarged, its walls are flabby, with a weakened tone and a response to massage. Recovery of its size to non-pregnant is delayed up to 35-45 days or more.

Chronic subinvolution of the uterus in cows is diagnosed 30 or more days after calving and is one of the main causes of cow infertility. It is characterized by an increase in the size of the uterus, a thickening of its walls, a decrease in tone and a weakening of the response to massage, and the absence of allocation of lochia. Chronic subinvolution of the uterus in cows is manifested both by multiple inconclusive inseminations and by anaphrodisia.

A known analogue is a method of treating uterine subinvolution in cows with a 7% solution of ichthyol in combination with carbacholine in the composition of the drug "Karbakhtiol" / 1 /. A drug for the treatment of uterine subinvolution, containing carbacholine, medical ichthyol, glucose and phenol in the following ratio, wt.%:

Carbacholine 0.005 Ichthyolum medical 7.0 Glucose 5,0 Phenol 0.1 Distilled water Rest

The disadvantage of this method of treatment is its low efficiency due to the fact that ichthyol, which is a means of general stimulating nonspecific therapy, enters the body of a sick animal in the composition of carbachthiol in a limited amount and does not lead to a high therapeutic effect.

A known analogue is a method of treating subinvolution of cows using the biostimulator "Placenta active principle" (PAN) / 2 /. It is made from the placenta of women without heat treatment using the effect of cavitation during sterilization. The disadvantage of this method is that for the treatment of uterine subinvolution, only a general stimulating therapy is used and uterine preparations are not used, and therefore, the therapeutic effectiveness of this method is low.

The closest technical solution is a method of treating uterine subinvolution in cows with the use of general stimulating nonspecific therapy - 7% ichthyol in a 0.85% solution of sodium chloride, uterine myotropic drugs - oxytocin and prostaglandin F2 _α - estufalan / 3 /.

The disadvantage of this method is the low efficiency of its use in the treatment of chronic subinvolution of the uterus in cows (1-2 or more after calving), as well as acute subinvolution of the uterus, which requires a second course of treatment after 12-14 days,

The technical result of the invention is to increase the effectiveness of the treatment of subinvolution of the uterus in cows, reducing the complexity, reducing the duration of infertility and the rate of fertilization.

The goal is achieved by the combined use of non-specific stimulating therapy (ichthyol and denatured emulsified placenta) for the treatment of uterine subinvolution together with symptomatic therapy (oxytocin, sinestrol, magestrofan).

The inventive method includes the use of means of general stimulating action - denatured emulsified placenta and ichthyol, symptomatic therapy - oxytocin, sinestrol and a preparation of the prostaglandin group F2 _α - magestrofan.

Ichthyol (bitaminol, ichthammol, ichthyol-ammonium, ichthiosulfol) is an ammonium salt of shale oil sulfonic acids. Ichthyol is black, in a thin layer of brown, syrupy liquid of a peculiar pungent odor, it dissolves in water, glycerin, partially in alcohol and ether. Ichthyol is a dry distillation product of bitumen shale containing the remains of fossil marine animals and fish. It acts antiseptically, antiparasitic, anti-inflammatory, keratoplastic and local anesthetizing. After intravenous administration, it acts on the principle of nonspecific therapy - annoyingly, in connection with which a number of reflex reactions develop, accompanied by an increase in the body's defenses, an increase in the secretion of adrenaline, serotonin and vasopressin, and narrowing of blood vessels and capillaries. With endometritis and uterine subinvolution in cows, ichthyol is used intramuscularly or subcutaneously in the form of a 7% solution in 40% glucose solution or in a 0.85% sodium chloride solution / 4 /.

Emulsified denatured placenta (PDE) is a tissue preparation made from human placenta. The drug is a sterile emulsion of a white or white with a yellowish tint color, specific odor, pH 2.1-2.7. PDE contains a complex of biologically active substances (peptides, nucleic acids, hexuronic acids, trace elements, etc.). The drug has an anti-inflammatory effect, has a positive effect on reparative processes, improves metabolism, stimulates sexual function, and increases the body's resistance. PDE is used to treat and prevent postpartum diseases in cows and sows. For prophylactic purposes, the drug is administered to cows twice immediately after birth and after 72 hours subcutaneously at a dose of 20 ml. With endometritis or mastitis, cows are administered PDE subcutaneously at a dose of 20 ml every 48-72 hours 5-7 times. The use of PDE does not exclude the use of other means / 5 /.

Oxytocin is an injection solution containing in 1 ml of 5 PIECES or 10 PIECES of a synthetic analog of the polypeptide hormone of the posterior pituitary gland. Oxytocin increases tone and enhances uterine smooth muscle contractions. It also causes a reduction in the myoepithelial cells surrounding the alveoli of the mammary gland, and has weak vasopressin-like, antidiuretic properties. Oxytocin is prescribed to agricultural and domestic animals for mild attempts during childbirth, retention of the placenta, uterine bleeding, atony, hypotension and inflammation of the uterus, mastitis, and also with reflex agalactia. The drug is administered subcutaneously, intramuscularly, intravenously / 6 /.

Sinestrol (hexestrol) - 4,4- (1,2-diethyl-1,2-ethanediyl) bisphenol (in the form of diacetate or didecanoate) is a clear, oily liquid of light yellow color. Sinestrol - a synthetic estrogen - a derivative of stilbene, estrogen is equivalent in estrogen activity. Together with the corpus luteum hormone, the follicular hormone is involved in the implementation of the menstrual cycle. Sinestrol increases the sensitivity of the uterine muscles to substances that excite their motor activity. Circulation of large amounts of estrogen in the blood for a short time promotes the ovulatory type of production of luteinizing hormone. Sinestrol is used for ovarian hypofunction, infertility, vaginitis, labor weakness / 7 /.

Magestrofan is a synthetic analogue of prostaglandin F2 _α (cloprostenol). Contains in 1 cm ³ solution of 0.25 mg of the active substance - cloprostenol. The drug is a colorless transparent solution. The active substance of magestrofan - cloprostenol has a specific luteolytic effect. Its use in the luteal phase of the estrous cycle causes resorption of the corpus luteum and, thus, creates the prerequisites for the onset of estrus and ovulation. The drug is administered intramuscularly. One of the indications for the use of the drug is the prevention and treatment of subinvolution of the uterus and endometritis. For these purposes, magestrofan is administered once to cows at a dose of 2 cm ³ 6-12 hours after calving. For therapeutic purposes, magestrofan is administered in the same doses in combination with symptomatic therapy / 8 /.

The novelty of the claimed method lies in the combined use of two drugs with a general stimulating effect - 7% ichthyol, denatured emulsified placenta (PDE). These drugs are used as follows. PDE is administered subcutaneously at a dose of 20 ml on the first, fifth and ninth days of a therapeutic course; sterile 7% ichthyol, prepared on a 0.85% sodium chloride solution, is injected three times subcutaneously in a heated animal body temperature, on the first, third and fifth days at doses of 25, 30 and 35 ml. At the same time, the animals are using the means of symptomatic therapy - oxytocin, sinestrol, magestrofan.

PDE is administered subcutaneously at a dose of 20 ml on the first, fifth and ninth days of a therapeutic course. Sterile 7% ichthyol prepared on a 0.85% sodium chloride solution is administered three times subcutaneously in the form of an animal heated to body temperature, on the first, third and fifth days at doses of 25, 30 and 35 ml. Oxytocin is administered subcutaneously at a dose of 8-10 IU / 100 kg of body weight four times, starting from the second day of treatment with a 24-hour interval in the treatment of acute and subacute subinvolution of the uterus and at a dose of 6-8 IU / 100 kg of animal body weight, three times with 24 hourly interval from the second day of the therapeutic course. Sinestrol (2% oil solution) is injected intramuscularly twice with a 24-hour interval at a dose of 2 ml from the first day of the therapeutic course in the treatment of acute and once in the treatment of subacute uterine subinvolution. If the treatment of subacute subinvolution begins from 20-22 days after calving, then instead of sinestrol, a preparation of the prostaglandin group F-2α - magestrofan in a dose of 2 ml is administered once intramuscularly on the first day of treatment. Magestrofan is administered intramuscularly at a dose of 2 ml on the first day of treatment for chronic uterine subinvolution.

Testing the effectiveness of the treatment method was carried out on cows of LLC Voronezhpishcheproduct Novousmansky and SHA "Levashovka" Anninsky areas of the Voronezh region.

The essence of the method is illustrated by examples.

Example 1

Justification of the complex use of ichthyol and placenta denatured emulsified

Cows with uterine subinvolution were injected with 7% sterile ichthyol, prepared on a 0.85% sodium chloride solution subcutaneously, warmed up to body temperature in doses of 25, 30 and 35 ml on the first, third and fifth days of treatment, PDE was injected subcutaneously in a dose of 20 ml on the first, fifth and ninth days of a therapeutic course. Before treatment and on day 9 from its beginning, blood was taken for immunobiological studies. The results of the experiment are presented in table 1.

Table 1 Justification of the complex use of ichthyol and placenta denatured emulsified (PDE). №№ Indicators Before treatment At the end of treatment 1. Bactericidal activity,% 83.9 93.5 2. Lysozyme activity, mcg / ml 0.63 ± 0.08 0.86 ± 0.08 ^* 3. Complementary activity,% 3.12 3.02 4. Phagocytic activity of leukocytes,% 56.6 87.5 5. Phagocytic number 11.5 ± 0.86 23.7 ± 1.12 ^*** 6. Phagocytic index 6.48 ± 0.50 20.7 ± 0.94 ^*** * - P <0.05

*** -P <0.001

From table 1 it is seen that the use of ichthyolotherapy in combination with a tissue preparation - denatured emulsified placenta leads to an increase in indicators of the general nonspecific resistance of the body. The use of these drugs contributes to an increase in the bactericidal activity of blood serum by 9.6%, lysozyme activity - by 36.5% (P <0.05), and the phagocytic activity of leukocytes - by 30.9%.

The combined use of ichthyol and a denatured emulsified placenta enhances cellular and humoral defense.

Example 2

The experiment was carried out on cows of red-motley breed of 3-6 years of age with a body weight of 450-550 kg. The diagnosis of acute uterine subinvolution was made on the basis of anamnestic data and obstetric-gynecological examination 6-7 days after calving.

Cows with acute uterine subinvolution were intramuscularly injected with a 2% Sinestrol oil solution in a dose of 2 ml twice from the first day of treatment with a 24-hour interval. On the first, fifth and ninth days, PDE was administered subcutaneously at a dose of 20 ml. Ichthyol was administered in the form of a 7% solution prepared on 0.85% sodium chloride, heated to the animal’s body temperature, with a 48-hour interval in doses of 25, 30 and 35 ml. Oxytocin at a dose of 8-10 U / 100 kg of animal body weight was injected subcutaneously four times with a 24-hour interval, starting from the second day of treatment.

Control animals were treated using the prototype. They were injected with 7% ichthyol, prepared on a 0.85% sodium chloride solution heated to animal body temperature with a 48-hour interval from the first day of treatment in increasing lowering doses: 20, 25, 30, 35, 30 and 25 ml. The same animals were intramuscularly injected with a 2% Sinestrol oil solution twice with a 24-hour interval from the first day of treatment at a dose of 2 ml. On the second, third, fourth and fifth days, oxytocin was administered subcutaneously at a dose of 8-10 ED / 100 kg body weight.

The animals underwent daily clinical examination, taking into account the time of disappearance of the clinical signs of the disease, the number of animals recovered and fertilized, the rate of fertilization, the length of time from calving to fertilization.

table 2 The therapeutic efficacy of the claimed and known methods of treating acute uterine subinvolution in cows. Indicators Using prototype Using the claimed method Number of animals, goal 23 25 Recovery time, days 13.9 ± 0.41 10.8 ± 0.28 Recover, goal twenty 24 Therapeutic efficacy,% 86.9 96.0 The period from calving to fertilization, days 62.1 ± 1.19 43.6 ± 0.87 Fertilized at the first insemination: goal 16 twenty % 69.5 80.0 Fertilization rate 1.61 ± 0.05 1.41 ± 0.05 Toxic effect, goal 0 0

From table 2 it follows that the best therapeutic effect in the treatment of acute subinvolution of the uterus is achieved with the combined use of ichthyol, denatured emulsified placenta and oxytocin against the background of sinestrol.

Example 3

The experiment was carried out on cows of red-motley breed of 3-6 years of age with a body weight of 450-550 kg. The diagnosis of subacute uterine subinvolution was made on the basis of medical history and obstetric-gynecological examination on 15-17 and 20-22 days after calving.

On the first day of treatment, cows with subacute subinvolution of the uterus (15-17 days after calving) were injected intramuscularly with a 2% Sinestrol oil solution at a dose of 2 ml. If treatment begins from 20-22 days after calving, then instead of synestrol, a preparation of the prostaglandin group F-2α - magestrofan in a dose of 2 ml is administered intramuscularly once on the first day of treatment. On the first, fifth and ninth days, PDE was administered subcutaneously at a dose of 20 ml. Ichthyol was administered in the form of a 7% solution prepared on 0.85% sodium chloride, heated to animal body temperature, on the first, third and fifth days at doses of 25, 30 and 35 ml. Oxytocin at a dose of 8-10 IU / 100 kg of animal body weight was injected subcutaneously four times with a 24-hour interval, starting from the second day of treatment

Control animals were treated using the prototype. They were injected with 7% ichthyol, prepared in a 0.85% sodium chloride solution, heated up to the animal’s body temperature, with a 48-hour interval from the first day of treatment in increasing-decreasing doses: 20, 25, 30, 35, 30 and 25 ml. The same animals were injected intramuscularly with a 2% Sinestrol oil solution on the first day of treatment at a dose of 2 ml. On the second, third, fourth and fifth days, oxytocin was administered subcutaneously at a dose of 8-10 ED / 100 kg body weight.

Table 3 The therapeutic efficacy of the claimed and known methods of treating subacute uterine subinvolution in cows. Indicators Using prototype Using the claimed method The number of sick animals eleven fifteen Clinical recovery,% 81.8 93.3 Fertilized Goal 7 12 % 63.6 80.0 The period from calving to fertilization, days 70.7 ± 4.39 56.7 ± 2.96 Fertilization rate 1.45 ± 0.28 1.33 ± 0.07

The animals underwent daily clinical examination, taking into account the time of disappearance of the clinical signs of the disease, the number of animals recovered and fertilized, the rate of fertilization, the length of time from calving to fertilization.

From table 3 it follows that the best therapeutic effect in the treatment of subacute subinvolution of the uterus is achieved with the combined use of ichthyol, denatured emulsified placenta and oxytocin against the background of sinestrol or magestrofan.

Example 4

The experiment was carried out on cows of red-motley breed of 3-6 years of age with a body weight of 450-550 kg. The diagnosis was made on the basis of anamnestic data and obstetric-gynecological examination 30-60 days after calving.

On the first day of the therapeutic course, cows were injected intramuscularly with magestrofan in a dose of 2 ml. On the first, fifth and ninth days, PDE was administered subcutaneously at a dose of 20 ml. Ichthyol was administered in the form of a 7% solution prepared on 0.85% sodium chloride, heated to animal body temperature, on the first, third and fifth days at doses of 25, 30 and 35 ml. On the second, third and fourth days of the course of treatment, oxytocin was administered subcutaneously at a dose of 6-8 U / 100 kg of animal body weight.

Control animals were treated using the prototype. They were injected with 7% ichthyol, prepared on a 0.85% sodium chloride solution heated to animal body temperature with a 48-hour interval from the first day of treatment in increasing-decreasing doses: 20, 25, 30, 35, 30 and 25 ml. The same animals were injected intramuscularly with magestrofan on the first day of treatment at a dose of 2 ml. On the second, third and fourth days, oxytocin was administered subcutaneously at a dose of 6-8 U / 100 kg body weight.

Animals were monitored, taking into account the number of recovered and fertilized animals, the duration of the period from the start of treatment to fertilization.

Table 4 The therapeutic efficacy of the claimed and known methods of treating chronic uterine subinvolution in cows. Indicators Using prototype Using the claimed method The number of sick animals 48 45 Clinical recovery,% (in 30 days) 87.3 95.3 Fertilized (for 3 months): goal 40 42 % 83.3 93.3 The period from the start of treatment to fertilization, days 32.7 ± 2.24 27.1 ± 1.94 Left Barren: Goal 8 3 % 16.3 6.6

From table 4 it follows that the best therapeutic effect was achieved with the combined use of ichthyol, denatured emulsified placenta, oxytocin and magestrofan.

Sources of information

1. Serebryakov Yu.M. A comprehensive system of measures to combat retention of the placenta and acute postpartum endometritis (Recommendations). - Novosibirsk, 1991, - 19 p.

2. Lobodin K.A. Clinical and morphological changes in the genitals and hormone-synthesizing function of the ovaries in highly productive dairy cows in the postpartum period / Abstract. dis .... cand. vet. sciences. - Voronezh, 2003, - 23 p.

3. Cheremisinov G.A., Azizyan R.N. Diagnosis and treatment of chronic subinvolution of the uterus in cows // Veterinary medicine. - 1989. - No. 10. - S. 40-43.

4. Chervyakov D.K. et al. Medicines in veterinary medicine. - M .: Kolos, 1977 .-- S.388-389.

5. Manual on the use of denatured emulsified placenta (PDE) in the treatment and prevention of postpartum diseases in cows and sows, Ministry of Agriculture of the Russian Federation, 05/13/1996

6. Guidance on the use of oxytocin solution in veterinary medicine. Ministry of Agriculture and Food of Russia, 10.23.1995

7. Instructions for use of the drug Sinestrol, Department of State Control of Quality, Efficiency, Safety of Medicines and Medical Equipment, 2000. Registration certificate 000310/01 - 2001.

8. Manual on the use of magestrofan, Ministry of Agriculture and Food of Russia, 03/22/2000

Claims (1)

A method of treating uterine subinvolution in cows, including the use of symptomatic therapy and general stimulating nonspecific therapy - 7% ichthyol in a 0.85% sodium chloride solution, characterized in that one more general stimulating nonspecific therapy is additionally used - denatured emulsified placenta (PDE), and the introduction of general stimulating non-specific therapy is carried out according to the following scheme: PDE is administered subcutaneously in a dose of 20 ml on the first, fifth and ninth days of therapeutic treatment sa; sterile 7% ichthyol, prepared in a 0.85% sodium chloride solution, is injected three times subcutaneously in an animal form warmed up to body temperature on the first, third and fifth days at doses of 25, 30, and 35 ml.