CN101966245B

CN101966245B - Compound injection for curing viral diseases in livestock and poultry and preparation method thereof

Info

Publication number: CN101966245B
Application number: CN201010505721A
Authority: CN
Inventors: 吴红云; 李小娜; 李建正
Original assignee: Zhengzhou Houyi Pharmaceutical Co Ltd
Current assignee: Henan Hou Yi Industry Group Co., Ltd.
Priority date: 2010-10-13
Filing date: 2010-10-13
Publication date: 2011-11-30
Anticipated expiration: 2030-10-13
Also published as: CN101966245A

Abstract

The invention discloses a compound injection for curing viral diseases in livestock and poultry and a preparation method thereof. 100ml of injection comprises the following components by weight: 5 to 15g of herba andrographitis extract, 1 to 5g of spreading hedvotis extract, 2 to 8g of taurine, 5 to 10g of propylene glycol, 0.05 to 0.1g of Tween-80, 0.1 to 0.4g of antioxygen and the balance of injection water. The compound injection for curing viral diseases in the livestock and the poultry in the invention is prepared from the pure traditional Chinese medicinal preparations including the herba andrographitis extract and the spreading hedvotis extract and has a good antivirus effect; the pure traditional Chinese medicinal ingredients have low toxic and side effects; and when the herba andrographitis and the spreading hedvotis are used in combination with the taurine, the medicinal effects of the herba and rographitis and the spreading hedvotis can be improved, and the dosage amount of the medicament can be reduced at the same time, so that the cost of the injection can be reduced.

Description

A kind of compound injection for the treatment of animal viral disease and preparation method thereof

Technical field

The present invention relates to a kind of compound injection for the treatment of the fowl bacterial disease, relate to a kind of preparation method of this injection simultaneously, belong to the veterinary drug technical field.

Background technology

Along with the development of intensive culture industry, animal viral disease not only serious harm herding is produced, and some zoonosis is also threatening human health.Because virus is strict cytozoicus, the virus of (outward) in the cell must optionally be destroyed or suppress to antiviral drugs, and pair cell does not produce fatal injury to the cell that does not infect at least.Yet present antiviral drugs major part is some Western medicine, they infect some diseases and have certain curative effect, but also produce many toxic and side effects simultaneously, also can produce certain damage to the cell that does not infect, for example forbidding such as ribavirin, amantadine medicine also remains incessant after repeated prohibition.Therefore present developing direction at viral disease is that curative effect is certain, cost is low, has no side effect again, reduces as far as possible and takes in the harm of medicine to animal or human's body.

Summary of the invention

The compound injection that the purpose of this invention is to provide a kind of good effect, toxic and side effects is low, cost is low treatment animal viral disease.

In order to realize above purpose; the technical solution adopted in the present invention is: a kind of compound injection for the treatment of animal viral disease; every 100ml injection mainly is made up of the component of following weight: Herba Andrographis extract 5～15g; Herba Hedyotidis Diffusae extract 1～5g; taurine 2～8g, propylene glycol 5～10g, tween 80 0.05～0.1g; antioxidant 0.1～0.4g, surplus is a water for injection.

Described Herba Andrographis extract is to be that solvent extraction forms with ethanol.

Described Herba Hedyotidis Diffusae extract is to be that solvent extraction forms with ethanol.

Described antioxidant is sodium sulfite, sodium sulfite or sodium thiosulfate.

The present invention also aims to provide a kind of preparation method for the treatment of the compound injection of animal viral disease, the step of this method is as follows:

1) gets Herba Andrographis and Herba Hedyotidis Diffusae, adopt alcohol deposition method to extract with ethanol respectively, obtain Herba Andrographis extract and Herba Hedyotidis Diffusae extract;

2) take by weighing the propylene glycol of 5～10g and the tween 80 of 0.05～0.1g, respectively the Herba Andrographis extract 5～15g of weighing and Herba Hedyotidis Diffusae extract 1～5g are dissolved wherein, obtain solution 1;

3) taurine that takes by weighing 2～8g adds the dissolving of injection water, regulates pH to 6.5～7.5, obtains solution 2;

4) sodium sulfite of getting 0.1～0.4g adds the injection dissolving of blunging, and obtains solution 3;

5) solution 1,2,3 is merged, add water for injection, regulate pH to 7-8, obtain injection to 100ml.

The compound injection of treatment animal viral disease of the present invention, wherein taurine can be regulated biological functions such as body normal physiological function, enhancing cell membrane antioxidation, free radical resisting damage and antiviral damage as the conditionality essential amino acids of animal.Injection of the present invention has also adopted the extract of Herba Andrographis and Herba Hedyotidis Diffusae two flavor Chinese medicines, Herba Andrographis bitter in the mouth, cold wherein, GUIXIN, lung, large intestine, urinary bladder channel have heat-clearing and toxic substances removing, the effect of reducing swelling and alleviating pain, Herba Hedyotidis Diffusae is a kind of traditional Chinese medicine, have heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling protects the liver and protects gallbladder, inducing diuresis to remove edema, the effect of anti-inflammation.Both share has good resistant function to virus, and pure Chinese medicine ingredients toxic and side effects is little, cooperates taurine to use, and the drug effect of Herba Andrographis and Herba Hedyotidis Diffusae is improved, and can also reduce the medicine dosage simultaneously, reduces the cost of injection.

The specific embodiment

Embodiment 1

The compound injection of the treatment animal viral disease of present embodiment is that the example its preparation method is as follows with 100ml:

1) get Herba Andrographis and Herba Hedyotidis Diffusae, adopt alcohol deposition method to extract with ethanol respectively: soak with ethanol, filtering and impurity removing adopts concentrating under reduced pressure method and freeze-drying then, obtains Herba Andrographis extract and Herba Hedyotidis Diffusae extract;

2) take by weighing the propylene glycol of 5g and the tween 80 of 0.05g, respectively the Herba Andrographis extract 5g of weighing and Herba Hedyotidis Diffusae extract 1g are dissolved wherein, obtain solution 1;

3) taurine that takes by weighing 2g adds the dissolving of injection water, regulates pH to 6.5, obtains solution 2;

4) sodium sulfite of getting 0.1g adds the injection dissolving of blunging, and obtains solution 3;

5) solution 1,2,3 is merged, add water for injection, regulate pH to 7, obtain injection to 100ml.

Embodiment 2

2) take by weighing the propylene glycol of 7.5g and the tween 80 of 0.08g, respectively the Herba Andrographis extract 10g of weighing and Herba Hedyotidis Diffusae extract 3g are dissolved wherein, obtain solution 1;

3) taurine that takes by weighing 5g adds the dissolving of injection water, regulates pH to 7, obtains solution 2;

4) sodium sulfite of getting 0.25g adds the injection dissolving of blunging, and obtains solution 3;

5) solution 1,2,3 is merged, add water for injection, regulate pH to 7.5, obtain injection to 100ml.

Embodiment 3

2) take by weighing the propylene glycol of 10g and the tween 80 of 0.1g, respectively the Herba Andrographis extract 15g of weighing and Herba Hedyotidis Diffusae extract 5g are dissolved wherein, obtain solution 1;

3) taurine that takes by weighing 8g adds the dissolving of injection water, regulates pH to 7.5, obtains solution 2;

4) sodium thiosulfate of getting 0.4g adds the injection dissolving of blunging, and obtains solution 3;

5) solution 1,2,3 is merged, add water for injection, regulate pH to 8, obtain injection to 100ml.

Experimental example

Compound injection medication of the present invention is intramuscular injection, and according to the poultry body weight, dose is 0.1ml/kg, and once-a-day, five is a course of treatment.

Below with concrete description of test drug effect of the present invention:

1 material and method:

1.1 test drug: the stable product of producing with 3 embodiment in this patent, No. 1, called after patent, No. 2, patent, No. 3, patent respectively.

1.2 experimental animal: make a definite diagnosis the sick chicken that suffers from newcastle disease and the sick duck of duck viral hepatitis

1.3 test grouping

This experiment will be suffered from poultry respectively and will be divided into 4 groups at random, No. 1 group of patent, No. 2 groups of patent, No. 3 groups of patent and blank group.Wherein the blank group is injected the normal saline of Isodose.

1.4 test method

According to 0.1ml/kg each group is suffered from poultry and inject, successive administration 5 days is observed situations such as suffering from poultry clinical change, healing, improvement, death, the line item of going forward side by side every day.

1.5 curative effect judging standard

Cure rate: after the off-test, sick pig transference cure, spirit takes a turn for the better, and appetite increases, and recovers the ratio that normal pig accounts for this group pig fully.

Inefficiency: symptom appears in duration of test, and the pig that still loses transference cure after the off-test accounts for the ratio of this group pig.

1.6 clinical test results sees Table 1,2.

The clinical therapeutic efficacy of table 1 pair newcastle disease

Group	Cure rate (%)	Inefficiency (%)
			Embodiment 1	87	15
Embodiment 2	92	5
			Embodiment 3	80	18
The blank group	–	–

The therapeutic effect of table 2 pair duck viral hepatitis

Group	Cure rate (%)	Inefficiency (%)
			Embodiment 1	91	9
Embodiment 2	93	6
			Embodiment 3	89	10
The blank group	–	–

2 conclusions

Injection of the present invention has significant curative effect to treatment newcastle disease and duck viral hepatitis, and wherein the therapeutic effect of embodiment 2 is the most remarkable.

Claims

1. compound injection for the treatment of animal viral disease; it is characterized in that: every 100ml injection is to be made by the component of following weight: be Herba Andrographis extract 5 ~ 15g that solvent extraction forms with ethanol; with ethanol is Herba Hedyotidis Diffusae extract 1 ~ 5g that solvent extraction forms; taurine 2 ~ 8g; propylene glycol 5 ~ 10g, tween 80 0.05 ~ 0.1g, antioxidant 0.1 ~ 0.4g; surplus is a water for injection, and described antioxidant is sodium sulfite, sodium sulfite or sodium thiosulfate.

2. preparation method for the treatment of the compound injection of animal viral disease according to claim 1, it is characterized in that: the preparation process of the injection of every 100ml is as follows:

2) take by weighing the propylene glycol of 5 ~ 10g and the tween 80 of 0.05 ~ 0.1g, respectively the Herba Andrographis extract 5 ~ 15g of weighing and Herba Hedyotidis Diffusae extract 1 ~ 5g are dissolved wherein, obtain solution 1;

3) taurine that takes by weighing 2 ~ 8g adds the dissolving of injection water, regulates pH to 6.5 ~ 7.5, obtains solution 2;

4) sodium sulfite of getting 0.1 ~ 0.4g adds the injection dissolving of blunging, and obtains solution 3;