RU2261718C2 - Method for correcting age-caused alterations in human immune system - Google Patents

Abstract

FIELD: medicine, gerontology.

SUBSTANCE: beforehand one should detect human biological age and the age of human immune system to compare them with human calendar age: in case of predominance of at least one of them against calendar age one should once introduce the suspension of human fetal tissues into subcutaneous fiber of anterior abdominal wall. For this purpose one should apply fetal tissues being heterogeneous by their histogenesis and, also, preparations of placental origin. The innovation provides normalization of immunocytogram values and, thus, similarity of calendar and biological age for 1 yr.

EFFECT: higher efficiency of correction.

Description

The invention relates to medicine, namely to gerontology, and can be used to correct age-related changes in the human immune system.

Recently, such a promising area of medicine as cell transplantation and transformation is actively developing, which allows stimulating the regeneration of affected tissues of individual organs and correcting the state of the human immune system based on the implantation of fetal cells and human tissues.

A known method of stimulating the regeneration of the affected liver, described in p. RF No. 2161036 "Method for the treatment of chronic diffuse liver diseases" according to cl. A 61 K 35/407, 35/48, 3.13.10.98, op. 12/20/2000

A known method for the treatment of chronic diffuse liver diseases is the introduction of human fetal tissues in the form of a suspension of live hepatocytes into the round ligament of the liver.

The disadvantage of this method is that it is highly targeted, because designed to stimulate the regeneration of only liver tissue.

Closest to the technical nature of the claimed is a method of stimulating tissue regeneration described in the article by Chistovoy L.V., Solkh M. et al. "Experience in the use of fetal human tissues in the treatment of children with chronic viral hepatitis and liver cirrhosis" in the collection of articles edited by . G.T.Suhikh "Transplantation of fetal tissues and human cells", M., International Institute of Biological Medicine, 1966, p. 84-86.

A known method for the treatment of chronic diffuse diseases of the liver is the introduction by injection of a suspension of human fetal tissues subcutaneously at 4-5 points of the anterior abdominal wall during a course of treatment, including 2-4 injections.

The disadvantage of this method is that it is not effective enough and has limited use, because Designed to regenerate only liver tissue.

The aim of the invention is to expand the capabilities of the method while increasing its effectiveness.

This goal is achieved by the fact that in the method of correction of age-related changes in the human immune system, which consists in introducing by injection into the region of the anterior abdominal wall a suspension of human fetal tissues, ACCORDING TO THE INVENTION, the biological age of the person and the age of his immune system are preliminarily determined and compared with the calendar age of the person and if at least one of them exceeds the calendar age of a person, a suspension of human fetal tissues is administered once, for which a heterogeneity is used Fetal tissues, as well as preparations of placental origin, which are histologically different, are injected into the subcutaneous tissue of the anterior abdominal wall.

Determining the difference in biological age and age of the immune system and comparing them with the calendar age makes it possible to identify age-related changes in the immune system that lead to premature aging of the body, and in conjunction with the use of injection of fetal tissues of different genesis and placental preparations produced in the subcutaneous tissue the anterior abdominal wall, makes it possible to stimulate the regeneration of tissues of various human organs and to correct age-related changes in the immune system human threads, preventing premature aging.

Compared with the prototype, the inventive method has novelty, differing from it by such essential features as determining the biological age and age of its immune system, and if at least one of them exceeds the calendar age, the use of heterogeneous histogenesis and preparations of placental origin for the treatment of fetal tissues, injection in the subcutaneous tissue of the anterior abdominal wall, which together ensure the achievement of a given result.

Although it is known that fetal tissues are used to stimulate the regeneration and treatment of individual human organs, the applicant is not aware of the technical solutions possessing the indicated distinguishing features that make it possible to obtain the indicated result; therefore, he considers that the claimed solution meets the criterion of "inventive step".

The inventive method can be widely used in medicine, in particular in gerontology, and therefore meets the criterion of "industrial applicability".

The inventive method is as follows.

The biological age of a person and the age of his immune system are determined, they are compared with the calendar age of a person, and if at least one of them is higher than the calendar age, a suspension of human fetal tissues is injected into the subcutaneous tissue of the anterior abdominal wall once, for which fetal heterogeneous histogenesis is used tissues, as well as preparations of placental origin.

The inventive method is as follows.

Using the BIOAGE software package (developed by the Scientific Research Institute of Gerontology, Kiev), the biological age of a person is determined. Immunological age is determined by methods of system analysis in groups.

All patients are divided into groups:

- the immunological age is less than the calendar (passport);

- immunological age is calendar;

- The immunological age is greater than the calendar.

In the group with a high immunological age, there was a more frequent incidence of catarrhal viral and bacterial infections (up to 6-8 times a year, in other groups 1-3 times), herpes of various localization (2 times more), etc. In women, an earlier onset of menopause (from 38-40 years old).

For transplantation, fetal tissues were taken from embryos up to 20 weeks of gestation obtained with "small" caesarean sections and late abortions for medical reasons. The fetotransplant was prepared on the basis of the biomedical center NPMO "Module".

Suspensions of liver, spleen, adrenal glands, and placental placenta (or ovaries) were prepared for administration, depending on the patient’s gender. Fetotransplantation was performed by injection into the region of the anterior abdominal wall once using a syringe with a volume of 10 ml of thick and thin injection needles with a diameter of 2 mm and 0.8 mm, respectively, 100 mm long. The patient in supine position after the usual treatment of the surgical field, perform local anesthesia of the skin and subcutaneous tissue with 0.25% novocaine solution on an area of 2 square meters. cm on the midline of the abdomen in the middle of the distance between the navel and the xiphoid process. A suspension of fetal tissues and preparations of placental origin is injected into the subcutaneous tissue of the anterior abdominal wall, for example, the left iliac region. After that, the needle is removed and an aseptic dressing is applied to the injection site.

The introduced embryonic cells have a powerful germinal potential; they contain biologically active substances that stimulate the regeneration of affected cells.

Re-examination was carried out after 1 month, 6 months, 1 year.

All patients showed normalization of immunogram indices, the immunological age corresponded to the calendar, in 45% it became less than the calendar. Half of the treated patients during the year have never had colds. All observed observed a subjective improvement in well-being, increased efficiency, improved skin turgor.

Clinical examples

Patient V., 63 years old.

At the initial treatment, the biological age is 68 years.

Complaints of frequent herpetic eruptions over the past two years on the lips, wings of the nose (1 time in 3-4 months), a marked decrease in performance, a decrease in potency. Over the past year, he suffered acute sinusitis three times, was treated with antibiotics. At the last examination, growth of the fungal flora was detected on the nasal mucosa, and underwent a course of antifungal therapy.

Suffers from osteochondrosis of the lumbosacral spine, hypertension 1 tbsp.

In the immunogram: white blood cells are reduced to 3.6 × 10 ⁹ / l, lymphocytes - 17%, CD3-45%, CD4 - 38%, CD8 - 20%, CD22 - 12%, IgA - 1.5 g / l, IgM - 1.75 g / l, IgG - 11 g / l, CH50 - 52.7, CEC - 78, the size of the CEC is large, the HCT test is spontaneous 12, induced - 17%.

Fetal tissues of the liver, testicles, spleen were transplanted.

In 6 months:

Biological age is 59 years.

Over the period of observation of herpetic eruptions, she does not note, the general condition has significantly improved, at the time of examination she takes only antihypertensive drugs. All indicators of the immunogram are within normal limits.

Patient V., 49 years old.

At the initial treatment, the biological age is 58 years.

Complaints of frequent colds (8 times this year), hair loss, tooth decay, fluctuations in blood pressure, general weakness. Suffers from ovarian exhaustion syndrome from 38 years old, is registered with a gynecologist, takes hormone replacement therapy.

In the immunogram: white blood cells 5.0 × 10 ⁹ / l, lymphocytes - 14%, CD3-40%, CD4 - 22%, CD8 - 14%, CD22 - 21%, IgA - 2.2 g / l, IgM - 0 , 70 g / l, IgG - 9 g / l, CH50 - 59.1, CEC - 66%, average sizes. HCT test spontaneous 7%, induced - 12%.

Transplantation of fetal tissues of the liver, testicles, placenta.

In 6 months:

Biological age 49 years.

During the observation period, she was never sick. Significantly reduced the dose of hormonal drugs. Notes improvement in skin turgor, hair loss has stopped, marked subjective improvement in well-being.

All indicators of the immunogram are normal.

After 12 months - no complaints, immunological parameters are normal. Biological age corresponds to the calendar.

Compared with the prototype, the claimed method for correcting age-related changes in the human immune system is more effective and has wider possibilities of use.

Claims (1)

A method for correcting age-related changes in the human immune system, which consists of introducing a suspension of human fetal tissues by injection into the anterior abdominal wall of a person, characterized in that the biological age of the person and the age of his immune system are preliminarily determined and compared with the calendar age of the person and if at least one of them, over a calendar age, a single suspension of human fetal tissues is administered, for which fetal tissues heterogeneous in their histogenesis are used, as well as preparations of placental origin, wherein the injection is carried into the subcutaneous tissue of the anterior abdominal wall.