RU2175553C1 - Method of prophylaxis of egg yield decrease syndrome - 76 birds - Google Patents

Abstract

FIELD: veterinary, poultry breeding. SUBSTANCE: method involves intramuscular or intranasal administration of zoolan in the dose 0.3-0.5 mg/kg of live mass being simultaneously or in 24 h after vaccination of birds. Method provides expressing immunity and enhancement of efficiency. EFFECT: improved method of prophylaxis. 5 tbl, 4 ex

Description

 The invention relates to veterinary medicine, in particular to methods for the prevention of adenoviral infections of birds and can be used for the prevention of egg laying syndrome - 76 (SSN - 76) chickens.

 Known methods for the prevention of SSW - 76 birds, including the use of vaccines, mainly inactivated (1).

 Known methods provide long-term (up to 10 months) immunity with a relatively high protective level.

 The purpose of the invention is to increase the preventive effectiveness of the method and productivity of chickens.

 This goal is achieved by the fact that on the background of vaccination, the drug "Zoolan" (Deltacin) is additionally administered.

 "Zoolan" (Deltacin) - is a natural non-peptide produced in the body with a stabilizing cellular homeostasis effect, anti-radical and membrane-stabilizing effect.

 "Zoolan" (Deltacin) is a synthetic nonapeptide (delta-sleep inducing peptide), has the following amino acid sequence: tryptophanyl - alanyl - glycyl - glycyl - aspartyl - alanyl - seryl - glycyl - glutamic acid.

 Studies conducted on cows, calves, pigs, cats and dogs for non-infectious (rhinitis, bronchitis, nephritis, paresis and paralysis of traumatic etiology, etc.), infectious (carnivorous plague, leptospirosis, viral hepatitis, parvovirus enteritis, respiratory diseases of mixed viral bacterial etiology ) and invasive (dog toxocariasis) diseases, showed the compatibility of Zoolan with various drugs and confirmed the possibility of its use against the background of the main treatment and prevention regimens (2).

 The method is as follows.

 Chickens at the same time as or 24 hours after the administration of the inactivated vaccine against SSN-76 are administered intranasally or intramuscularly zoolan in a dose of 0.3-0.5 mg / kg body weight.

 The proposed method meets the criterion of "novelty", as it is characterized by the presence of distinctive features from the prototype.

 It also meets the criterion of "inventive step", since the combination of distinctive features that gives the object of the invention properties that provide a positive effect in the available sources of patent and scientific and technical information is not installed. The introduction of zoolan normalizes the stress and immune response, as well as the homeostasis of the body due to increased synthesis of growth hormone and inhibition of excessive production of glucocorticosteroids.

 Considering that the reactivation of the pathogen SSY-76 occurs after the contaminated bird reaches full puberty under the influence of stress caused by the physiological reorganization of the laying hens before the most active stage of oviposition, the stress-protective effect of zoolan in addition to immunomodulatory increases the protective level.

 Studies substantiating the parameters of the method and confirming its effectiveness were carried out in the conditions of the Balakhninsky poultry farm (Nizhny Novgorod region) on a 110-day-old bird of the Isa-Brown breed.

 The essence of the method is illustrated by examples.

 Example 1. Justification of the dose. 5 groups of chickens were formed, which were kept under identical conditions on a similar diet. Chickens of all groups were vaccinated against SSN-76 intramuscularly in the right thigh with the introduction of a 0.5 ml dose of inactivated Osidze vaccine at the same time in the first 4 groups zoolan was administered intramuscularly in the left thigh at a dose of 0.3, 0.4, 0.5, 0.6 mg / kg body weight. Efficacy was assessed by the strength of the immune system, for which the titer of antibodies to the SSJ-76 virus was determined in the reaction of delayed hemagglutination (RHCA) on the 21st day after vaccination. The clinical condition of the bird and the reaction to the introduction of zoolan were taken into account. The results are presented in table 1.

 As can be seen from table 1, the indicators of immunity in the experimental groups, regardless of the dose of zoolan, are higher than in the control, but increasing the dose is not economically feasible, since there was a tendency to decrease it compared to the optimal doses (groups 2 and 4).

 Example 2. The rationale for the route of administration. Formed 4 groups of chickens, which were kept under identical conditions on a similar diet. Chickens of all groups were vaccinated, as in example 1. 24 hours after vaccination, zoolan was used in the first 3 groups at a dose of 0.4 mg / kg body weight: in the first group, intramuscularly in the left thigh, in the 2nd and 3rd groups — by the intranasal spray method, large droplets, and in group 2, the zoolan preparation was not stabilized with glycerol, and in the 3rd group, the zoolan drug was stabilized with glycerol at the rate of 10% of the solution volume.

 Efficiency was evaluated as in example 1. The results are presented in table 2.

 As can be seen from table 2, in the experimental groups the indicators of immunity are significantly higher than in the control. Due to its simplicity, intranasal administration by spray method with stabilization of zoolane solution with glycerol at the rate of 10% of the solution volume can be considered preferable in practical conditions.

 Example 3. The rationale for the time of administration. 3 groups of chickens were formed, which were kept under identical conditions on a similar diet. Chickens of all groups were vaccinated, as in example 1. In the first two groups, zoolan stabilized with glycerol at the rate of 10% of the solution volume, at a dose of 0.4 mg / kg body weight, was applied intranasally by the spray method, after 24 hours after vaccination, in the 2nd group - simultaneously with vaccination. Efficiency was evaluated as in example 1. The results are presented in table 3.

 As can be seen from table 3, the time of administration of zoolan did not affect the parameters of immunity relative to the control group, the best indicators were obtained with the simultaneous administration of a vaccine and zoolan. Concomitant administration of zoolan with the vaccine is preferred.

 Example 4. Confirmation of effectiveness. 6 groups of chickens were formed, which were kept under identical conditions on a similar diet. In 3 groups, SSN-76 prophylaxis was carried out by known methods using inactivated vaccines (Osidze, a vaccine manufactured by ARRIAH, Binyuvaksidrop vaccine (France) against infectious bronchitis, Newcastle disease and SSN-76) in the other 3 groups - the proposed method with intranasal spray method the introduction of zoolan in a dose of 0.4 mg / kg body weight. Zoolan in the form of a 0.05% solution stabilized with glycerol at the rate of 10% of the solution volume at a dose of 0.8 ml simultaneously with the introduction of the vaccine. Additionally, taking into account the intensity of the immune system, productivity was calculated according to the increase in live weight for 70 days and egg productivity for 31 days.

 The results are presented in tables 4 and 5.

 As can be seen from tables 4 and 5, the proposed method provided a significant increase in indicators of the intensity of immunity (for example, the geometric mean titers of antibodies increased by 1.4-2.6 times) and productivity, which confirmed the effectiveness of the proposed method.

 The proposed method is feasible and effective for intramuscular or intranasal administration simultaneously with the vaccine or 24 hours after its administration using zoolan regardless of the vaccine.

 It is preferable to administer zoolane simultaneously with the intranasal spray method.

 Zoolan was well tolerated regardless of the route of administration, which was confirmed by the absence of a local skin reaction with intramuscular and normal respiratory function with intranasal administration. Mobility, behavior, food intake and reaction to external stimuli were the same in the hens of the experimental and control groups.

Sources of information taken into account
1. R.N. Korovin, I.K. Christmas. Adenoviral infections of poultry. - M.: Agropromizdat, 1990, p. 49-72.

 2. Veterinary practice, N 3, 1997, p. 22-29.4

Claims (1)

 A method for the prevention of a decrease in egg production-76 syndrome by administering a vaccine, characterized in that zoolan is administered intramuscularly or intranasally at a dose of 0.3-0.5 mg / kg body weight simultaneously or 24 hours after vaccination.

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