CN104208677A - Mixed freeze-dried powder for preventing chicken Newcastle disease, infectious bronchitis, egg drop syndrome, and bird flu, and preparation method thereof - Google Patents
Mixed freeze-dried powder for preventing chicken Newcastle disease, infectious bronchitis, egg drop syndrome, and bird flu, and preparation method thereof Download PDFInfo
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Abstract
The invention discloses mixed freeze-dried powder for preventing chicken Newcastle disease, infectious bronchitis, egg drop syndrome, and bird flu, and a preparation method thereof. The mixed freeze-dried powder comprises the following components: a quadruple yolk antibody for preventing chicken Newcastle disease, infectious bronchitis, egg drop syndrome, and bird flu, a quadruple transfer factor for preventing chicken Newcastle disease, infectious bronchitis, egg drop syndrome, and bird flu, an antiseptic, an inactivating agent, selenium, vitamin C, polyvinylpyrrolidone, and glycine. The mixed freeze-dried powder is prepared by compounding a quadruple yolk antibody and a quadruple transfer factor, at the same time a plurality of auxiliary materials are added to enhance the effect, the components cooperate with each other, and thus the comprehensive controlling effect on chicken Newcastle disease, infectious bronchitis, egg drop syndrome, and bird flu is prominently improved. The freeze-dried powder has the advantages of long preservation time at a normal temperature, high antibody titer, and good bio-activity, and is a high effective biological preparation with a wide application prospect.
Description
Technical field
The invention belongs to veterinary drug technical field, be specifically related to the freeze-dried mixed powder of a kind of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu, also relate to the preparation method of freeze-dried mixed powder of a kind of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu simultaneously.
Background technology
Newcastle disease (Newcastle Disease, ID) has another name called philippine fowl disease or pseudo-fowl plague, the high degree in contact caused by paramyxovirus, acute, septic infectious disease.The principal character that this disease occurs be dyspnea, just rare, neurological disorders, mucosa and serous coat are hemorrhage, mortality rate is high, serious to poultry husbandry harm.First nineteen twenty-six is found in Indonesia, and find at Britain's new city again soon, all there is popular record countries in the world.
Infectious bronchitis of chicken (IB) is the acute high degree in contact respiratory infectious disease of one of the chicken caused by infectious bronchitis virus, and it faces examines feature and be dyspnea, send rale, cough, mouth breathing, sneeze etc.Laying hen infects that this occurs that egg production reduces after being ill usually, degradation symptom under the quality of egg.This disease is widely current in all over the world, is the important epidemic disease of poultry husbandry.
Egg-decreasing syndrome (Egg Drop Syndrome, EDS) egg drop syndrome is again, cause commodity laying hen and Breeding hens laying rate to decline by adenovirus or laying rate does not reach a kind of reproductive system infectious disease of fertility performance index, main manifestations for mass-sending property laying rate reduce, eggshell is abnormal, egg body deformity, the low of inferior quality disease of egg matter.
Bird flu (Avian Influenza, AI) has another name called true fowl plague, the deadly infectious disease of a kind of Major Epidemic in chicken group caused by bird flu virus (AIV); Its highly pathogenicity strain can cause birds sudden death, is the category-A epidemic disease that International Office of Epizootics specifies, also can infects people.Bird flu betides the Italy of 1878 the earliest.At that time, there is chicken group mortality in Italy, was called as fowl plague at that time.To nineteen fifty-five, scientist confirms that its Causative virus is influenza A virus, and after this, this disease is renamed as bird flu.
Prevent and treat at present newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu method more, mainly use Western medicine, immunoprophylaxis, Chinese herbal medicine etc. to prevent and treat.Wherein, spreading unchecked that Western medicine uses brings great potential safety hazard to Pork food, and drug residue has become one of principal element affecting pork product safety.Immunoprophylaxis early stage results is better, but needs repeatedly to inoculate, troublesome poeration, and cost is higher, easily causes stress; And vaccine requires higher for storage conditions, need cold preservation under 2-8 DEG C of condition, room temperature condition lower a period of time then loses effect.Chinese herbal feed additive is also one of current research direction, but because the kind of Chinese herbal medicine is more, identical Chinese crude drug is very large to the difference between the effects of different diseases of bird and livestock, and Chinese herbal medicine is extremely limited to the immune effect of disease, therefore applies and also has certain limitation.Summary of the invention
The object of this invention is to provide the compositions of a kind of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu, solve the problem that operation is inconvenient, medicament is not easily preserved, effect is poor that prior art prevents and treats newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu existence simultaneously.
Second object of the present invention is to provide the freeze-dried mixed powder of a kind of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu.
3rd object of the present invention is to provide the preparation method of freeze-dried mixed powder of a kind of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu.
In order to realize above object, the technical solution adopted in the present invention is: a kind of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the freeze-dried mixed powder of bird flu, comprise the component of following parts by weight: anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody 94 ~ 97 parts, anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad transfer factor 1 ~ 2 part, antiseptic 0.01 ~ 0.02 part, inactivator 0.2 ~ 0.3 part, 0.05 ~ 0.1 part, selenium, vitamin C 0.5 ~ 1 part, polyvinylpyrrolidone 1 ~ 2 part, glycine 0.5 ~ 1 part.
Described inactivator is formaldehyde.
Described antiseptic is thimerosal.
A preparation method for the freeze-dried mixed powder of above-mentioned anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu, comprises the following steps:
A. newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu virus type strain is adopted to prepare quadruple vaccine, immune health chicken group obtains high-immunity egg, by the supernatant that high-immunity egg obtains containing anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody through sterilization, acidification, centrifugal treating;
B. the supernatant containing anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody is mixed with anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad, antiseptic, inactivator, selenium, vitamin C, polyvinylpyrrolidone, glycine, then lyophilizing and get final product.
Preferably, the preparation method of the freeze-dried mixed powder of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu, comprises the following steps:
1) get newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu virus type strain breed, its deactivation is prepared into quadruple vaccine, at interval of 7 days, immunity is carried out to healthy laying hen group, immunity three times altogether, start to detect antibody titer after second time immunity, when antibody titer reaches 1:256, collect high-immunity egg, the high-immunity egg of collection is carried out clear up, sterilize, dry for subsequent use;
2) separation of step 1) gained high-immunity egg is obtained high-immunity egg yolk, add isopyknic sterilized water, fully stir, obtain yolk antibody solution, in yolk antibody solution, add acidified aqueous solution, mix, staticly settle, get supernatant for subsequent use;
3) in step 2) add sodium salt in gained supernatant and make sodium salt volume account for 0.8% of cumulative volume, centrifugalize, the supernatant of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody must be contained;
4) supernatant of the anti-newcastle disease containing 94 ~ 97 weight portions of step 3) gained, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody is got, add 1 ~ 2 weight portion anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad, filter, reserved filtrate is for subsequent use;
5) step 4) gained filtrate is concentrated into 2.5ml/500 plumage part, add antiseptic 0.01 ~ 0.02 weight portion, inactivator 0.2 ~ 0.3 weight portion, selenium 0.05 ~ 0.1 weight portion, vitamin C 0.5 ~ 1 weight portion, polyvinylpyrrolidone 1 ~ 2 weight portion, glycine 0.5 ~ 1 weight portion mix, first-20 DEG C of pre-coolings, lyophilizing under-80 DEG C of conditions, to obtain final product again.
Step 2) described in the pH of acidified aqueous solution be 4.9 ~ 5.2; The addition of acidified aqueous solution is 8 times of yolk antibody liquor capacity.Described acidified aqueous solution is acidified aqueous solution.
Sodium salt described in step 3) is the mass ratio of NaCl and NaSO4 is the mixture of 4:1.
The rotating speed of centrifugalize described in step 3) is 15000rpm, and the time is 20min.
Filtering described in step 4) is first filter with the filter membrane of 0.45 μm, and molecular cut off is greater than the macromole of 200KD, then by the membrane filtration of filtrate with 0.22 μm.
Described anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad, prepared by following methods:
1), after immunity being carried out to pig spleen and/or thymus with newcastle disease, infectious bronchitis, egg-decreasing syndrome, avian influenza virus antigen, its surperficial muscle, fat is removed, then with the normal saline flushing after sterilizing;
2) by after the pig spleen after flushing and/or thymus fragmentation, the PBS buffer after the pre-cooling of 2 times of volumes is added, with tissue homogenate instrument, it is homogeneous, obtain homogenate;
3) by homogenate with after Ultrasonic Cell Disruptor process 5 ~ 10min, regulate pH be 5.5, centrifugal 30min under 5000 ~ 6000rpm rotating speed, collect supernatant;
4) by step 3) gained supernatant micro-filtrate membrane filtration, molecular cut off is the material of more than 7000 dalton, gained filtrate is used degerming membrane filtration, obtain anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad transfer factor stock solution, save backup under 4 DEG C of conditions.
Yolk antibody is a kind of immunoglobulin, due to body is subject to the stimulation of external antigen and the one that produces stress product, can combine with corresponding antigens, eliminate the cause of disease.The present invention is by the chicken group of the quadruple vaccine immunity inoculation health of newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu, can make in the egg yolk of acquisition, to produce effective anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody, decrease the probability of strain reversion simultaneously.
Transfer factor carries the specific immunity information of primed lymphocyte, can by specific immunity information in passing recipient lymphocytes, the lymphocyte of receptor non-activity is made to change specificity primed lymphocyte into, thus the immunoreation exciting recipient cell to mediate.
In the freeze-dried mixed powder of anti-newcastle disease of the present invention, infectious bronchitis, egg-decreasing syndrome, bird flu, anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody are a kind of immunoglobulins, have good prevention effect to newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu; Anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad itself are a kind of special antiviral agents, both are combined, greatly can improve comprehensive prevention and the effect for the treatment of newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu.
Anti-newcastle disease of the present invention, infectious bronchitis, egg-decreasing syndrome, the freeze-dried mixed powder of bird flu, by anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody and anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad is carried out composite, and be aided with antiseptic, inactivator, selenium, vitamin C, freeze-dried mixed powder prepared by polyvinylpyrrolidone and glycine, each component cooperatively interacts, coordinative role, its integrated control newcastle disease can be significantly improved, infectious bronchitis, egg-decreasing syndrome, the effect of bird flu, by force pointed, the room temperature lower holding time is long, antibody titer is high, the advantage that biological activity is good, it is a kind of potent biological preparation with wide application prospect, prove through animal experiment, the freeze-dried mixed powder of anti-newcastle disease of the present invention, infectious bronchitis, egg-decreasing syndrome, bird flu, anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody obviously will be better than, any one situation about being used alone in anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad to the integrated control effect of newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu.
The freeze-dried mixed powder of anti-newcastle disease of the present invention, infectious bronchitis, egg-decreasing syndrome, bird flu, during use, can be used as feed additive and is mixed in feedstuff by mass fraction 2% ~ 3%; Adopt the administration of intramuscular injection mode during morbidity, containing freeze-dried mixed powder 1g in every 100ml injection, the use amount of injection is each 0.1 ~ 0.2ml/kg body weight; Medication first doubles, and therapeutic effect is better.
Anti-newcastle disease of the present invention, infectious bronchitis, egg-decreasing syndrome, the preparation method of the freeze-dried mixed powder of bird flu, by anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody, anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad and multiple auxiliary materials carry out composite, and by adjusting the composition of adjuvant, control its consumption, extend the holding time of yolk antibody further, ensure that the activity in the yolk antibody long period at normal temperatures, reduce the storage and transport cost of freeze-dried powder preparation, easy to use, be with a wide range of applications, preparation method technique is simple, easy to operate, greatly reduces production cost, is applicable to large-scale industrial production.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further illustrated.
In the specific embodiment of the present invention, newcastle disease used, infectious bronchitis, egg-decreasing syndrome, bird flu virus are national standard strain.
In the specific embodiment of the present invention, anti-newcastle disease used, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad, prepared by following methods:
1), after immunity being carried out to pig spleen and/or thymus with newcastle disease, infectious bronchitis, egg-decreasing syndrome, avian influenza virus antigen (commercially available), its surperficial muscle, fat is removed, then with the normal saline flushing after sterilizing;
2) by after the pig spleen after flushing and/or thymus fragmentation, the PBS buffer after the pre-cooling of 2 times of volumes is added, with tissue homogenate instrument, it is homogeneous, obtain homogenate;
3) by homogenate with after Ultrasonic Cell Disruptor process 5 ~ 10min, regulate pH be 5.5, centrifugal 30min under 5000 ~ 6000rpm rotating speed, collect supernatant;
4) by step 3) gained supernatant micro-filtrate membrane filtration, molecular cut off is the material of more than 7000 dalton, gained filtrate is used degerming membrane filtration, obtain anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad transfer factor stock solution, save backup under 4 DEG C of conditions.
Embodiment 1
The freeze-dried mixed powder of the anti-newcastle disease of the present embodiment, infectious bronchitis, egg-decreasing syndrome, bird flu, comprises the component of following parts by weight: anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody 94 parts, anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad transfer factor 2 parts, thimerosal 0.02 part, 0.2 part, formaldehyde, 0.05 part, selenium, vitamin C 1 part, polyvinylpyrrolidone 2 parts, glycine 0.5 part.
The preparation method of the freeze-dried mixed powder of the anti-newcastle disease of the present embodiment, infectious bronchitis, egg-decreasing syndrome, bird flu, comprises the following steps:
1) get newcastle disease (lasota), infectious bronchitis, egg-decreasing syndrome (EDS57), bird flu (HP) Virus Standard strain breed, its deactivation is prepared into quadruple vaccine, at interval of 7 days, immunity is carried out to healthy laying hen group, immunity three times altogether, first time, immune health chicken group inoculated inactivated vaccine 1.5ml, for the second time, third time immunity inoculation dosage 1 times, start to detect antibody titer after second time immunity, when antibody titer reaches 1:256, collect high-immunity egg, the high-immunity egg of collection is carried out clear up, sterilize, dry for subsequent use;
2) separation of step 1) gained high-immunity egg is obtained high-immunity egg yolk, add isopyknic sterilized water, abundant stirring, obtain yolk antibody solution, the acidified aqueous solution that pH is 5.0 is added in yolk antibody solution, acidified aqueous solution addition is 8 times of yolk antibody liquor capacity, mixes, staticly settles, get supernatant for subsequent use;
3) in step 2) to add in gained supernatant by NaCl and NaSO4 be mixing sodium salt that the ratio of 4:1 is mixed to form in mass ratio, mixing sodium salt volume is made to account for 0.8% of cumulative volume, with the centrifugal 20min of the rotating speed of 15000rpm, the supernatant of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody must be contained;
4) supernatant of the anti-newcastle disease containing 94 weight portions of step 3) gained, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody is got, add 2 weight portion anti-newcastle diseases, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad, filter with the filter membrane of 0.45 μm, the molecular weight that dams is greater than the macromolecular substances of 200KD, again by filtrate through 0.22 μm of membrane filtration, reserved filtrate is for subsequent use;
5) step 4) gained filtrate is concentrated into 2.5ml/500 plumage part, add thimerosal 0.02 weight portion, formaldehyde 0.2 weight portion, selenium 0.05 weight portion, vitamin C 1 weight portion, polyvinylpyrrolidone 2 weight portion, glycine 0.5 weight portion mix, first-20 DEG C of pre-coolings, lyophilizing under-80 DEG C of conditions, to obtain final product again.
Embodiment 2
The freeze-dried mixed powder of the anti-newcastle disease of the present embodiment, infectious bronchitis, egg-decreasing syndrome, bird flu, comprises the component of following parts by weight: anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody 95 parts, anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad transfer factor 1.5 parts, thimerosal 0.01 part, 0.3 part, formaldehyde, 0.1 part, selenium, vitamin C 0.5 part, polyvinylpyrrolidone 1.5 parts, glycine 1 part.
The preparation method of the freeze-dried mixed powder of the anti-newcastle disease of the present embodiment, infectious bronchitis, egg-decreasing syndrome, bird flu, comprises the following steps:
1) get newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu virus standard strain breed, its deactivation is prepared into quadruple vaccine, at interval of 7 days, immunity is carried out to healthy laying hen group, immunity three times altogether, first time, immune health chicken group inoculated inactivated vaccine 1.5ml, for the second time, third time immunity inoculation dosage 1 times, start to detect antibody titer after second time immunity, when antibody titer reaches 1:256, collect high-immunity egg, the high-immunity egg of collection is carried out clear up, sterilize, dry for subsequent use;
2) separation of step 1) gained high-immunity egg is obtained high-immunity egg yolk, add isopyknic sterilized water, abundant stirring, obtain yolk antibody solution, the acidified aqueous solution that pH is 5.0 is added in yolk antibody solution, acidified aqueous solution addition is 8 times of yolk antibody liquor capacity, mixes, staticly settles, get supernatant for subsequent use;
3) in step 2) to add in gained supernatant by NaCl and NaSO4 be mixing sodium salt that the ratio of 4:1 is mixed to form in mass ratio, mixing sodium salt volume is made to account for 0.8% of cumulative volume, with the centrifugal 20min of the rotating speed of 15000rpm, the supernatant of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody must be contained;
4) supernatant of the anti-newcastle disease containing 95 weight portions of step 3) gained, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody is got, add 1.5 weight portion anti-newcastle diseases, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad, filter with the filter membrane of 0.45 μm, the molecular weight that dams is greater than the macromolecular substances of 200KD, again by filtrate through 0.22 μm of membrane filtration, reserved filtrate is for subsequent use;
5) step 4) gained filtrate is concentrated into 2.5ml/500 plumage part, add thimerosal 0.01 weight portion, formaldehyde 0.3 weight portion, selenium 0.1 weight portion, vitamin C 0.5 weight portion, polyvinylpyrrolidone 1.5 weight portion, glycine 1 weight portion mix, first-20 DEG C of pre-coolings, lyophilizing under-80 DEG C of conditions, to obtain final product again.
Embodiment 3
The freeze-dried mixed powder of the anti-newcastle disease of the present embodiment, infectious bronchitis, egg-decreasing syndrome, bird flu, comprises the component of following parts by weight: anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody 97 parts, anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad transfer factor 1 part, thimerosal 0.01 part, 0.25 part, formaldehyde, 0.08 part, selenium, vitamin C 1 part, polyvinylpyrrolidone 1 part, glycine 0.5 part.
The preparation method of the freeze-dried mixed powder of the anti-newcastle disease of the present embodiment, infectious bronchitis, egg-decreasing syndrome, bird flu, comprises the following steps:
1) get newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu virus standard strain breed, its deactivation is prepared into quadruple vaccine, at interval of 7 days, immunity is carried out to healthy laying hen group, immunity three times altogether, first time, immune health chicken group inoculated inactivated vaccine 1.5ml, for the second time, third time immunity inoculation dosage 1 times, start to detect antibody titer after second time immunity, when antibody titer reaches 1:256, collect high-immunity egg, the high-immunity egg of collection is carried out clear up, sterilize, dry for subsequent use;
2) separation of step 1) gained high-immunity egg is obtained high-immunity egg yolk, add isopyknic sterilized water, abundant stirring, obtain yolk antibody solution, the acidified aqueous solution that pH is 5.0 is added in yolk antibody solution, acidified aqueous solution addition is 8 times of yolk antibody liquor capacity, mixes, staticly settles, get supernatant for subsequent use;
3) in step 2) to add in gained supernatant by NaCl and NaSO4 be mixing sodium salt that the ratio of 4:1 is mixed to form in mass ratio, mixing sodium salt volume is made to account for 0.8% of cumulative volume, with the centrifugal 20min of the rotating speed of 15000rpm, the supernatant of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody must be contained;
4) supernatant of the anti-newcastle disease containing 97 weight portions of step 3) gained, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody is got, add 1 weight portion anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad, filter with the filter membrane of 0.45 μm, the molecular weight that dams is greater than the macromolecular substances of 200KD, again by filtrate through 0.22 μm of membrane filtration, reserved filtrate is for subsequent use;
5) step 4) gained filtrate is concentrated into 2.5ml/500 plumage part, add thimerosal 0.01 weight portion, formaldehyde 0.25 weight portion, selenium 0.08 weight portion, vitamin C 1 weight portion, polyvinylpyrrolidone 1 weight portion, glycine 0.5 weight portion mix, first-20 DEG C of pre-coolings, lyophilizing under-80 DEG C of conditions, to obtain final product again.
Experimental example 1
This experimental example carries out Performance Detection to the freeze-dried mixed powder of embodiment 1 ~ 3 gained, and result is as shown in table 1,2.
Room temperature bioactivity experimental technique: the freeze-dried mixed powder preparation of embodiment 1 ~ 3 gained is kept in 37 DEG C of incubators, preserve 24 months, respectively 1 week, 2 weeks, January, February, April, August, December, after 16 months, 20 months, 24 months, by agar diffusion (AGP) method, the yolk antibody measuring each time point institute sample thief is tired change.
Table 1 embodiment 1 ~ 3 gained freeze-dried mixed powder room temperature bioactivity experimental result
The freeze-dried mixed powder room temperature of table 2 embodiment 1 ~ 3 gained places performance test results
Experimental example 2
The freeze-dried mixed powder of this experimental example to the anti-newcastle disease of embodiment 1 ~ 3, infectious bronchitis, egg-decreasing syndrome, bird flu prevent and treat newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu effect carry out clinical experiment.
Experimental technique: choose 1 the monthly age chicken 700, wherein 100 as the isolated rearing of counteracting toxic substances group, 100 as the isolated rearing of blank group, 200 are divided into 2 groups as a control group, often organize 100, isolated rearing, use the preparation for treating of comparative example 1,2 respectively, other 300 are divided into 3 groups as test group, often organize 100, use the preparation for treating of embodiment 1,2,3 respectively.Feeding manner is free choice feeding normal diet, freely drinks water, and after 24h, get newcastle disease, infectious bronchitis, egg-decreasing syndrome, the avian influenza venom after the pig intramuscular injection inoculation dilution of test group, matched group and counteracting toxic substances group, inoculum concentration is 2ml/; Start immunotherapy targeted autoantibody after infection, intramuscular injection, injection dosage is 0.2ml/kg body weight; Death condition after record 120h, result is as shown in table 3.
The freeze-dried mixed powder of table 3 embodiment 1 ~ 3 gained is to the prevention effect of newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu
The medicament of comparative example 1 is made up of the component of following parts by weight: anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody 97 parts, thimerosal 0.01 part, 0.25 part, formaldehyde, 0.08 part, selenium, vitamin C 0.8 part, polyvinylpyrrolidone 1.5 parts, glycine 0.5 part.
The medicament of comparative example 2 is made up of the component of following parts by weight: anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad transfer factor 2 parts, thimerosal 0.01 part, 0.25 part, formaldehyde, 0.08 part, selenium, vitamin C 0.8 part, polyvinylpyrrolidone 1.5 parts, glycine 0.5 part.
As can be seen from Table 3, the freeze-dried mixed powder of embodiment 1 ~ 3 gained can effectively prevent and treat newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu simultaneously, and freeze-dried mixed powder effect obviously will be better than any one situation about being used alone in anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody, anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad.
Claims (10)
1. the freeze-dried mixed powder of an anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu, it is characterized in that: the component comprising following parts by weight: anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody 94 ~ 97 parts, anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad transfer factor 1 ~ 2 part, antiseptic 0.01 ~ 0.02 part, inactivator 0.2 ~ 0.3 part, 0.05 ~ 0.1 part, selenium, vitamin C 0.5 ~ 1 part, polyvinylpyrrolidone 1 ~ 2 part, glycine 0.5 ~ 1 part.
2. the freeze-dried mixed powder of anti-newcastle disease according to claim 1, infectious bronchitis, egg-decreasing syndrome, bird flu, is characterized in that: described inactivator is formaldehyde.
3. the freeze-dried mixed powder of anti-newcastle disease according to claim 1, infectious bronchitis, egg-decreasing syndrome, bird flu, is characterized in that: described antiseptic is thimerosal.
4. a preparation method for the freeze-dried mixed powder of anti-newcastle disease as claimed in claim 1, infectious bronchitis, egg-decreasing syndrome, bird flu, is characterized in that: comprise the following steps:
A. newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu virus type strain is adopted to prepare quadruple vaccine, immune health chicken group obtains high-immunity egg, by the supernatant that high-immunity egg obtains containing anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody through sterilization, acidification, centrifugal treating;
B. the supernatant containing anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody is mixed with anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad, antiseptic, inactivator, selenium, vitamin C, polyvinylpyrrolidone, glycine, then lyophilizing and get final product.
5. the preparation method of the freeze-dried mixed powder of anti-newcastle disease according to claim 4, infectious bronchitis, egg-decreasing syndrome, bird flu, is characterized in that: comprise the following steps:
1) get newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu virus type strain breed, its deactivation is prepared into quadruple vaccine, at interval of 7 days, immunity is carried out to healthy laying hen group, immunity three times altogether, start to detect antibody titer after second time immunity, when antibody titer reaches 1:256, collect high-immunity egg, the high-immunity egg of collection is carried out clear up, sterilize, dry for subsequent use;
2) separation of step 1) gained high-immunity egg is obtained high-immunity egg yolk, add isopyknic sterilized water, fully stir, obtain yolk antibody solution, in yolk antibody solution, add acidified aqueous solution, mix, staticly settle, get supernatant for subsequent use;
3) in step 2) add sodium salt in gained supernatant and make sodium salt volume account for 0.8% of cumulative volume, centrifugalize, the supernatant of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody must be contained;
4) supernatant of the anti-newcastle disease containing 94 ~ 97 weight portions of step 3) gained, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad yolk antibody is got, add 1 ~ 2 weight portion anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad, filter, reserved filtrate is for subsequent use;
5) step 4) gained filtrate is concentrated into 2.5ml/500 plumage part, add antiseptic 0.01 ~ 0.02 weight portion, inactivator 0.2 ~ 0.3 weight portion, selenium 0.05 ~ 0.1 weight portion, vitamin C 0.5 ~ 1 weight portion, polyvinylpyrrolidone 1 ~ 2 weight portion, glycine 0.5 ~ 1 weight portion mix, first-20 DEG C of pre-coolings, lyophilizing under-80 DEG C of conditions, to obtain final product again.
6. the preparation method of the freeze-dried mixed powder of anti-newcastle disease according to claim 5, infectious bronchitis, egg-decreasing syndrome, bird flu, is characterized in that: step 2) described in the pH of acidified aqueous solution be 4.9 ~ 5.2; The addition of acidified aqueous solution is 8 times of yolk antibody liquor capacity.
7. the preparation method of the freeze-dried mixed powder of anti-newcastle disease according to claim 5, infectious bronchitis, egg-decreasing syndrome, bird flu, is characterized in that: sodium salt described in step 3) is the mass ratio of NaCl and NaSO4 is the mixture of 4:1.
8. the preparation method of the freeze-dried mixed powder of anti-newcastle disease according to claim 5, infectious bronchitis, egg-decreasing syndrome, bird flu, is characterized in that: the rotating speed of centrifugalize described in step 3) is 15000rpm, and the time is 20min.
9. the preparation method of the freeze-dried mixed powder of anti-newcastle disease according to claim 5, infectious bronchitis, egg-decreasing syndrome, bird flu, it is characterized in that: filtering described in step 4) is first filter with the filter membrane of 0.45 μm, molecular cut off is greater than the macromole of 200KD, then by the membrane filtration of filtrate with 0.22 μm.
10. the preparation method of the freeze-dried mixed powder of the anti-newcastle disease according to claim 4 or 5, infectious bronchitis, egg-decreasing syndrome, bird flu, it is characterized in that: described anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the transfer factor of bird flu tetrad, prepared by following methods:
1), after immunity being carried out to pig spleen and/or thymus with newcastle disease, infectious bronchitis, egg-decreasing syndrome, avian influenza virus antigen, its surperficial muscle, fat is removed, then with the normal saline flushing after sterilizing;
2) by after the pig spleen after flushing and/or thymus fragmentation, the PBS buffer after the pre-cooling of 2 times of volumes is added, with tissue homogenate instrument, it is homogeneous, obtain homogenate;
3) by homogenate with after Ultrasonic Cell Disruptor process 5 ~ 10min, regulate pH be 5.5, centrifugal 30min under 5000 ~ 6000rpm rotating speed, collect supernatant;
4) by step 3) gained supernatant micro-filtrate membrane filtration, molecular cut off is the material of more than 7000 dalton, gained filtrate is used degerming membrane filtration, obtain anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, bird flu tetrad transfer factor stock solution, save backup under 4 DEG C of conditions.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106267194A (en) * | 2016-08-25 | 2017-01-04 | 青岛润达生物科技有限公司 | A kind of yolk antibody and Swine spleen transfer factor joint product |
| CN106310251A (en) * | 2016-08-23 | 2017-01-11 | 河北中贝佳美生物科技有限公司 | Anti-virus and IgY antibody improving chicken formula and preparing method thereof |
| RU2624503C1 (en) * | 2015-12-30 | 2017-07-04 | Маргарита Евгеньевна Дмитриева | Way to prevent viral diseases of birds |
| EP3957713A4 (en) * | 2019-04-19 | 2023-01-25 | FUJIFILM Corporation | Storage stabilizer for extracellular vesicle and storage stabilization method for extracellular vesicle |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101607083A (en) * | 2008-06-18 | 2009-12-23 | 洛阳普莱柯生物工程有限公司 | A kind of preparation method of bird flu tetrad inactivated vaccine |
| CN102038949A (en) * | 2010-12-29 | 2011-05-04 | 青岛易邦生物工程有限公司 | Method for producing newcastle disease, infectious bronchitis, egg drop syndrome and avian influenza (H9 subtype) combined inactivated vaccine |
| CN102166355A (en) * | 2010-02-25 | 2011-08-31 | 乾元浩生物股份有限公司 | Quadruple inactivated vaccine for preventing chicken diseases |
-
2013
- 2013-09-30 CN CN201310462825.0A patent/CN104208677B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101607083A (en) * | 2008-06-18 | 2009-12-23 | 洛阳普莱柯生物工程有限公司 | A kind of preparation method of bird flu tetrad inactivated vaccine |
| CN102166355A (en) * | 2010-02-25 | 2011-08-31 | 乾元浩生物股份有限公司 | Quadruple inactivated vaccine for preventing chicken diseases |
| CN102038949A (en) * | 2010-12-29 | 2011-05-04 | 青岛易邦生物工程有限公司 | Method for producing newcastle disease, infectious bronchitis, egg drop syndrome and avian influenza (H9 subtype) combined inactivated vaccine |
Non-Patent Citations (2)
| Title |
|---|
| 刘兴民等: "抗鸡传染性法氏囊病-新城疫～传染性支气管炎-大肠杆菌病四联高免卵黄抗体的研制与应用", 《内蒙古兽医》 * |
| 日穆德玛等: "乌鸡转移因子对鸡新城疫Ⅱ系鸡传染性支气管炎H_(120)二联活疫苗协同作用的初步观察", 《中国兽医杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2624503C1 (en) * | 2015-12-30 | 2017-07-04 | Маргарита Евгеньевна Дмитриева | Way to prevent viral diseases of birds |
| CN106310251A (en) * | 2016-08-23 | 2017-01-11 | 河北中贝佳美生物科技有限公司 | Anti-virus and IgY antibody improving chicken formula and preparing method thereof |
| CN106267194A (en) * | 2016-08-25 | 2017-01-04 | 青岛润达生物科技有限公司 | A kind of yolk antibody and Swine spleen transfer factor joint product |
| EP3957713A4 (en) * | 2019-04-19 | 2023-01-25 | FUJIFILM Corporation | Storage stabilizer for extracellular vesicle and storage stabilization method for extracellular vesicle |
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