CN113730552B - Composition for improving physiological functions of livestock and poultry and preparation method thereof - Google Patents

Composition for improving physiological functions of livestock and poultry and preparation method thereof Download PDF

Info

Publication number
CN113730552B
CN113730552B CN202111096924.2A CN202111096924A CN113730552B CN 113730552 B CN113730552 B CN 113730552B CN 202111096924 A CN202111096924 A CN 202111096924A CN 113730552 B CN113730552 B CN 113730552B
Authority
CN
China
Prior art keywords
composition
parts
livestock
improving
transfer factor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202111096924.2A
Other languages
Chinese (zh)
Other versions
CN113730552A (en
Inventor
张英杰
张雍禾
张本然
白怡霖
赵霞
张玉杰
徐超
陈龙
张琦杰
谭旭信
靳坤霞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henan Debang Biotechnology Co ltd
Zhengzhou Debang Bioengineering Co ltd
Original Assignee
Henan Debang Biotechnology Co ltd
Zhengzhou Debang Bioengineering Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henan Debang Biotechnology Co ltd, Zhengzhou Debang Bioengineering Co ltd filed Critical Henan Debang Biotechnology Co ltd
Priority to CN202111096924.2A priority Critical patent/CN113730552B/en
Publication of CN113730552A publication Critical patent/CN113730552A/en
Application granted granted Critical
Publication of CN113730552B publication Critical patent/CN113730552B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/07—Retinol compounds, e.g. vitamin A
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/191—Carboxylic acids, e.g. valproic acid having two or more hydroxy groups, e.g. gluconic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/28—Compounds containing heavy metals
    • A61K31/315—Zinc compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A61K31/355—Tocopherols, e.g. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/59—Compounds containing 9, 10- seco- cyclopenta[a]hydrophenanthrene ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/74—Synthetic polymeric materials
    • A61K31/785—Polymers containing nitrogen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/04—Sulfur, selenium or tellurium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/062—Ascomycota
    • A61K36/064—Saccharomycetales, e.g. baker's yeast
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Reproductive Health (AREA)
  • Physiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medical Informatics (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Gynecology & Obstetrics (AREA)
  • Pregnancy & Childbirth (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to the technical field of livestock breeding, in particular to a composition for improving physiological functions of livestock and a preparation method thereof. The composition is prepared by compounding sheep placenta transfer factor, compound vitamin, yeast selenium, calcium gluconate, zinc gluconate, gamma-polyglutamic acid and D-mannitol serving as active ingredients according to a certain mass ratio, and can be used as a feed additive to be added into drinking water or directly injected. The components are synergistic, the growth performance of young birds and young animals is improved, the death rate of the young birds and young animals is reduced, the growth rate is improved, the immunity of the organism is improved, the immune response of the organism to common vaccines such as newcastle disease and avian influenza is improved, the capability of producing effective antibodies after the organism is injected with the vaccines is improved, and the antiviral capability of the organism is improved; meanwhile, the composition can effectively improve the production performances of the egg fowls in the egg producing period, the milk yield of cows, the milk producing period and the like.

Description

Composition for improving physiological functions of livestock and poultry and preparation method thereof
Technical Field
The invention relates to the technical field of livestock breeding, in particular to a composition for improving physiological functions of livestock and a preparation method thereof.
Background
Livestock and poultry cultivation occupies an important proportion in agricultural economy in China, and is an dominant industry in traditional agriculture in China. Livestock and poultry cultivation is an economic source for farmers and is related to the healthy development of the society of China. The livestock and poultry farming industry in China is gradually developed to the large-scale and standardized directions. Under the traditional industrialized cultivation mode, the livestock and poultry receive invasion of various pathogenic microorganisms, under normal conditions, the organism immunity of the livestock and poultry can resist the invasion of the pathogenic microorganisms, along with the reduction of immunity, the possibility that the pathogen invades the organism of the livestock and poultry is increased, and meanwhile, the immune system cannot generate normal immune response, so that the morbidity of the livestock and poultry is increased, and serious economic loss is generated.
Therefore, people realize that the production performances of reducing the casualties of young birds and young animals, improving the physiological functions of the young birds and young animals, reducing the risks of illness, improving the growth and development rate, improving the egg producing period of the egg birds, the milk producing amount of the dairy cows, the milk producing period and the like are important directions of increasing income. There are also many vaccine products or feed additive products on the market for reducing virus or bacteria infection, improving the physiological function of livestock and poultry, improving the growth and development rate and increasing the egg and milk yields. However, with the development of economy and the improvement of the living standard of people, people are particularly favored for green, safe and pollution-free pure natural foods, and especially for meat, eggs and dairy products with more strict quality requirements. In order to simultaneously meet the improvement of economic benefits of livestock and poultry cultivation, and ensure the green, healthy, pollution-free and drug residue-free quality of meat, eggs and dairy products, people start to develop products which can be repeatedly added and used in the whole growth period of livestock and poultry cultivation and can improve the comprehensive physiological functions of livestock and poultry, and the purposes of safety, greenness, decomposability and no drug resistance and drug residue are achieved.
The transfer factor is a substance released by lymphocytes and capable of transferring sensitization information, does not contain protein, is a small molecular polypeptide, contains more than 6 amino acids, has the molecular weight of different animals and different antigen-specific transfer factors, and has certain differences in peptide molecular weight and amino acid types. The transfer factor has no heat source, the activity of the transfer factor is not destroyed by pancreatin, but the transfer factor is unstable to heat, and can specifically transfer the cellular immunity information of the donor to the receptor, thereby enhancing the immune function of the receptor. In addition to the wide application in human medicine, transfer factors have been widely used in recent years in products such as additives for enhancing immunity of livestock and poultry. How to select transfer factors with proper sources according to specific product function requirements, how to improve the effective utilization rate of organisms to the transfer factors and the comprehensive performance of products becomes a technical problem to be solved urgently at present.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a composition for improving the physiological functions of livestock and poultry, which is compounded with transfer factors, compound vitamins, various trace elements, polyglutamic acid and the like, so as to improve the growth performance of young birds and young animals, and the production performance of the young birds in the egg producing period, the milk producing amount of cows, the milk producing period and the like.
Meanwhile, the invention also provides a preparation method of the composition for improving the physiological functions of the livestock and poultry.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a composition for improving the physiological functions of livestock and poultry is prepared from the following active ingredients in parts by weight: 35-45 parts of sheep placenta transfer factor, 6-12 parts of compound vitamin, 0.8-1.2 parts of yeast selenium, 8-12 parts of calcium gluconate, 8-12 parts of zinc gluconate, 15-25 parts of gamma-polyglutamic acid and 8-12 parts of D-mannitol.
Further preferably, the composition for improving the physiological functions of livestock and poultry is prepared from the following active ingredients in parts by weight: 40 parts of sheep placenta transfer factor, 9 parts of compound vitamin, 1 part of yeast selenium, 10 parts of calcium gluconate, 10 parts of zinc gluconate, 20 parts of gamma-polyglutamic acid and 10 parts of D-mannitol.
Preferably, in a specific embodiment of the present invention, the multivitamin is prepared from vitamin a, vitamin D and vitamin E according to 1:1:1 mass ratio and compounding.
Optionally, the composition is in a form of freeze-dried powder, and can be in a form of microcapsule granules or other forms according to the requirements of practical application scenes.
The preparation method of the composition for improving the physiological functions of the livestock and poultry comprises the following operation steps:
1) Size mixing: taking the components of each active ingredient according to the parts by weight, adding sterile normal saline, and uniformly mixing to obtain composition slurry;
2) And (3) forming: the preparation method comprises the steps of molding and preparing the composition.
Preferably, in step 1), the ratio of feed to liquid is 1: and adding physiological saline in a proportion of 0.8-1.2.
Optionally, preparing transfer factor of placenta caprae seu ovis before pulping, including removing fat and coating from fresh placenta caprae seu ovis, mashing, grinding, crushing by ultrasonic cells, repeatedly freezing and thawing after crushing cells, centrifuging to obtain supernatant, filtering, retaining molecule to obtain transfer factor solution below 1000 dalton, measuring concentration of transfer factor, and taking transfer factor solution according to the mass fraction of placenta caprae seu ovis transfer factor in the composition. Preferably, the cells are broken and then freeze-thawed 3 times repeatedly.
Preferably, in the step 1), after adding physiological saline, uniformly mixing at 2-8 ℃ and adjusting the pH=6.5-7.5 to obtain the composition slurry.
Preferably, the step 2) of forming comprises the step of taking the composition slurry prepared in the step 1) to carry out freeze-drying treatment to prepare the composition freeze-dried powder for improving the physiological functions of the livestock and poultry.
The composition for improving the physiological functions of livestock and poultry is prepared by compounding sheep placenta transfer factors, compound vitamins, yeast selenium, calcium gluconate, zinc gluconate, gamma-polyglutamic acid and D-mannitol serving as effective components according to a certain mass ratio, and can be used as a feed additive or added into drinking water or injected. The components are synergistic, the growth performance of young birds and young animals is improved, the death rate of the young birds and young animals is reduced, the growth rate is improved, the immunity of the organism is improved, the immune response of the organism to common vaccines such as newcastle disease and avian influenza is improved, the capability of producing effective antibodies after the organism is injected with the vaccines is improved, and the antiviral capability of the organism is improved; meanwhile, the composition can effectively improve the production performances of the egg fowls in the egg producing period, the milk yield of cows, the milk producing period and the like.
The preparation method of the composition is simple and convenient to operate, easy to control and suitable for industrial production.
Detailed Description
The technical scheme of the invention is described in detail through specific examples.
The placenta transfer factor of sheep used in the following examples and comparative examples was prepared by the following method:
taking fresh sheep placenta, removing fat and envelope, mashing, grinding, crushing cells by ultrasonic, repeatedly freezing and thawing for 3 times, centrifuging to obtain supernatant, filtering, intercepting molecules to obtain transfer factor solution below 1000 dalton, and determining the concentration of transfer factor contained in the transfer factor solution.
The vitamins, selenium yeast, calcium gluconate, zinc gluconate, gamma-polyglutamic acid and D-mannitol used in the following examples and comparative examples are all commercially available products; wherein the gamma-polyglutamic acid is prepared by fermenting bacillus subtilis.
Example 1
A composition for improving the physiological functions of livestock and poultry is prepared from the following active ingredients in parts by weight: 40 parts of sheep placenta transfer factor, 3 parts of vitamin A, 3 parts of vitamin D, 3 parts of vitamin E, 1 part of yeast selenium, 10 parts of calcium gluconate, 10 parts of zinc gluconate, 20 parts of gamma-polyglutamic acid and 10 parts of D-mannitol.
The preparation method of the composition comprises the following specific operation steps:
1) Size mixing: the preparation method comprises the steps of taking the components of each active ingredient according to the mass portion, and according to the feed liquid ratio of 1:1, adding sterile physiological saline, uniformly mixing at 2-8 ℃, and adjusting pH=7.0 to obtain composition slurry;
2) And (3) forming: and (3) carrying out freeze-drying treatment on the composition slurry prepared in the step (1) to prepare the composition freeze-dried powder for improving the physiological functions of the livestock and poultry.
Example 2
A composition for improving the physiological functions of livestock and poultry is prepared from the following active ingredients in parts by weight: 35 parts of sheep placenta transfer factor, 4 parts of vitamin A, 4 parts of vitamin D, 4 parts of vitamin E, 1.2 parts of yeast selenium, 8 parts of calcium gluconate, 12 parts of zinc gluconate, 15 parts of gamma-polyglutamic acid and 8 parts of D-mannitol.
The preparation method of the composition comprises the following specific operation steps:
1) Size mixing: the preparation method comprises the steps of taking the components of each active ingredient according to the mass portion, and according to the feed liquid ratio of 1: adding sterile physiological saline into 0.8, uniformly mixing at 28 ℃, and adjusting pH=6.5 to obtain composition slurry;
2) And (3) forming: and (3) carrying out freeze-drying treatment on the composition slurry prepared in the step (1) to prepare the composition freeze-dried powder for improving the physiological functions of the livestock and poultry.
Example 3
A composition for improving the physiological functions of livestock and poultry is prepared from the following active ingredients in parts by weight: 45 parts of sheep placenta transfer factor, 2 parts of vitamin A, 2 parts of vitamin D, 2 parts of vitamin E, 0.8 part of yeast selenium, 12 parts of calcium gluconate, 8 parts of zinc gluconate, 25 parts of gamma-polyglutamic acid and 12 parts of D-mannitol.
The preparation method of the composition comprises the following specific operation steps:
1) Size mixing: the preparation method comprises the steps of taking the components of each active ingredient according to the mass portion, and according to the feed liquid ratio of 1:1.2, adding sterile physiological saline, uniformly mixing at 2-8 ℃, and adjusting pH=7.5 to obtain composition slurry;
2) And (3) forming: and (3) carrying out freeze-drying treatment on the composition slurry prepared in the step (1) to prepare the composition freeze-dried powder for improving the physiological functions of the livestock and poultry.
Comparative example 1
The composition provided in this comparative example was different from that in example 1 in that gamma-polyglutamic acid was omitted and the amount of placenta caprae seu ovis transfer factor was increased to 60 parts, otherwise as in example 1.
Comparative example 2
The composition provided in this comparative example was different from that in example 1 in that selenium yeast was omitted and the sheep placenta transfer factor was increased by 50 parts, otherwise as in example 1.
Comparative example 3
The composition provided in this comparative example was different from that in example 1 in that the amounts of selenium yeast, zinc gluconate and calcium gluconate were adjusted to 11 parts of selenium yeast, 5 parts of zinc gluconate and 5 parts of calcium gluconate, otherwise the same as in example 1.
Test example 1
(1) Test composition lyophilized powder:
lyophilized powder of composition prepared in example 1 and comparative examples 1 to 3
(2) Test animals and groups
6000 broilers just hatched are randomly and averagely divided into five groups of 1200 broilers, wherein each group is A, B, C, D, E groups, wherein group A corresponds to the composition freeze-dried powder of the example 1, group B corresponds to the composition freeze-dried powder of the example 1, group C corresponds to the composition freeze-dried powder of the example 2, group D corresponds to the composition freeze-dried powder of the example 3, and group E is a blank control group
(3) Test method
(3.1) test field: cooperation pasture of Jinyuan agriculture and animal husbandry development limited company in jin city
(3.2) feeding method: the method comprises the steps of normally breeding all groups of tested broilers, immunizing newcastle disease and avian influenza, and feeding corresponding composition freeze-dried powder twice per day by a concentrated drinking water way on the 7 th day, 21 st day and 28 th day in each test group, wherein a blank control group is fed with an equal amount of physiological saline, and the feeding amount of the composition freeze-dried powder is 0.5mg/kg;
(3.3) data statistics:
the initial average weight of 100 tested broilers in each group is randomly weighed and marked, the same 100 average weights are weighed again after feeding for 30 days (if death occurs, the tested broilers with the same weight are randomly supplemented as supplementary sampling), and the average daily gain is calculated;
survival = number of surviving/original number of each group of tested broilers after 40 days of feeding;
immunizing each group of tested broiler for 7 days, 21 days and 28 days after feeding, respectively immunizing newcastle disease and avian influenza H7N9 vaccine, randomly extracting blood samples of 100 surviving tested broiler, and detecting newcastle disease antibody titer of the collected blood samples by adopting an ELISA method after feeding for 30 days; monitoring the level of the avian influenza H7N9 antibody in blood by adopting an indirect hemagglutination method; (3.4) test results:
statistical results of body weight and survival data for each test group are shown in table 1 below:
TABLE 1
The number of broiler chickens tested for each of the test groups for different antibody titers of newcastle disease is shown in table 2 below:
TABLE 2
The results of the avian influenza H7N9 antibody titer monitoring for each test group are shown in table 3 below:
TABLE 3 Table 3
It should be noted that the composition lyophilized powder provided in examples 2 and 3 of the present invention was verified to have substantially the same improvement of physiological functions of chicks as in example 1 according to the same test method as described above.
Conclusion: the data shown in tables 1 and 2 show that the effective components in the freeze-dried powder of the composition provided by the invention exert a synergistic effect, so that the growth rate of chicks is improved, the survival rate is improved, and the immune response effect to vaccines and the like is improved.
Test example 2
(1) Test composition lyophilized powder:
lyophilized powder of composition prepared in example 1 and comparative examples 1 to 3
(2) Test animals and groups
The average of the newly born 400 piglets is divided into five groups at random, 80 piglets are respectively A1, B1, C1, D1 and E1, wherein the group A1 corresponds to the composition freeze-dried powder of the example 1, the group B1 corresponds to the composition freeze-dried powder of the example 1, the group C1 corresponds to the composition freeze-dried powder of the example 2, the group D1 corresponds to the composition freeze-dried powder of the example 3 and the group E1 is a blank control group
(3) Test method
(3.1) test field: cooperation pasture of Jinyuan agriculture and animal husbandry development limited company in jin city
(3.2) feeding method: performing normal breeding of each group of tested piglets, performing swine fever vaccine injection epidemic prevention at the age of 25-30 days, and injecting corresponding composition freeze-dried powder into each test group on the 1 st day, the 10 th day, weaning and the transfer, wherein the blank control group is injected with an equivalent amount of physiological saline, and the injection amount of the composition freeze-dried powder is 0.5mg/kg;
(3.3) data statistics:
the initial average weight of each tested piglet and the average weight after 50 days of feeding are weighed, and the average daily gain is calculated;
survival = number of surviving/number of original for each group of piglets;
respectively injecting swine fever vaccine for 15 days and 30 days, randomly selecting 20 tested piglets in each group, and monitoring the antibody level in blood by adopting an indirect hemagglutination method;
(3.4) test results:
statistical results of body weight and survival data for each test group are shown in table 4 below:
TABLE 4 Table 4
The results of 15 days after injection of the swine fever vaccine are shown in table 5 below, and the results of 30 days are shown in table 6 below: wherein the qualification rate of the antibody titer is the number of piglets/total number of piglets with the antibody titer reaching the effective protection effect
TABLE 5
TABLE 6
It should be noted that the improvement of physiological functions of piglets by the composition freeze-dried powder provided in the examples 2 and 3 according to the present invention was verified to be substantially equivalent to that of the example 1 according to the same test method as described above.
Conclusion: the data shown in tables 3, 4 and 5 show that the effective components in the freeze-dried powder of the composition provided by the invention exert a synergistic effect, so that the growth rate of piglets is improved, the survival rate is improved, and the immune response effect to vaccines and the like is improved.
Test example 3
(1) Test composition lyophilized powder:
lyophilized powder of composition prepared in example 1 and comparative examples 1 to 3
(2) Test animals and groups
500 laying hens entering the egg producing period are randomly divided into five groups of A2, B2, C2, D2 and E2, and 100 laying hens are in each group, wherein the group A2 corresponds to the composition freeze-dried powder of the example 1, the group B2 corresponds to the composition freeze-dried powder of the example 1, the group C2 corresponds to the composition freeze-dried powder of the example 2, the group D2 corresponds to the composition freeze-dried powder of the example 3 and the group E2 is a blank control group
(3) Test method
(3.1) test field: cooperation pasture of Jinyuan agriculture and animal husbandry development limited company in jin city
(3.2) test procedure and statistics:
feeding the corresponding composition freeze-dried powder to each tested layer chicken of each test group by drinking water every 10 days, wherein the consumption of the composition freeze-dried powder is 0.5mg/kg, and counting the average time for improving the egg laying period of each composition freeze-dried powder test group compared with a control group, wherein the result is group A2 for 25 days; group B2 for 10 days; group C2 for 10 days; group D2 for 5 days.
Test example 4
(1) Test composition lyophilized powder:
lyophilized powder of composition prepared in example 1 and comparative examples 1 to 3
(2) Test animals and groups
Selecting 500 cows, randomly dividing into five groups of A3, B3, C3, D3 and E3, wherein 100 cows are in each group, wherein the group A3 corresponds to the composition freeze-dried powder of the example 1, the group B3 corresponds to the composition freeze-dried powder of the example 1, the group C3 corresponds to the composition freeze-dried powder of the example 2, the group D3 corresponds to the composition freeze-dried powder of the example 3 and the E3 is a blank control group
3. Test method
3.1 Test site: cooperation pasture of Jinyuan agriculture and animal husbandry development limited company in jin city
3.2 Test procedure and statistical results:
the tested dairy cows of each test group are injected with corresponding composition freeze-dried powder every 10 days, the dosage of the composition freeze-dried powder is 0.5mg/kg, and compared with a control group, the average milk yield of each composition freeze-dried powder test group is improved, and the result is 7% of the A3 group; group B3 5%; group C3, 4%; d3 group 2%;
counting the time for improving the milk peak period of each composition freeze-dried powder test group on average compared with a control group, wherein the result is A3 group for 15 days; group B3 for 12 days; group C3 for 10 days; group D3 for 5 days.
The test results of the test examples 3 and 4 show that the effective components in the freeze-dried powder of the composition provided by the invention exert a synergistic effect, prolong the egg producing period of the egg fowls and the milk producing period of the cows, and improve the milk producing amount of the cows.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (10)

1. The composition for improving the physiological functions of the livestock and the poultry is characterized by being prepared from the following active ingredients in parts by weight: 35-45 parts of sheep placenta transfer factor, 6-12 parts of compound vitamin, 0.8-1.2 parts of yeast selenium, 8-12 parts of calcium gluconate, 8-12 parts of zinc gluconate, 15-25 parts of gamma-polyglutamic acid and 8-12 parts of D-mannitol.
2. The composition for improving the physiological functions of livestock and poultry according to claim 1, which is characterized by being prepared from the following active ingredients in parts by weight: 40 parts of sheep placenta transfer factor, 9 parts of compound vitamin, 1 part of yeast selenium, 10 parts of calcium gluconate, 10 parts of zinc gluconate, 20 parts of gamma-polyglutamic acid and 10 parts of D-mannitol.
3. The composition for improving the physiological functions of livestock and poultry according to claim 1 or 2, wherein the sheep placenta transfer factor is prepared by the following method: taking fresh sheep placenta to remove fat and envelope, mashing and grinding, crushing cells by ultrasonic, repeatedly freezing and thawing after crushing the cells, centrifuging to obtain supernatant, filtering, and obtaining transfer factor solution below 1000 daltons through molecular interception.
4. The composition for improving physiological functions of livestock and poultry according to claim 1 or 2, wherein the compound vitamin consists of vitamin a, vitamin D and vitamin E according to 1:1:1 mass ratio and compounding.
5. The composition for improving physiological functions of livestock and poultry according to claim 1 or 2, wherein the formulation of the composition is freeze-dried powder.
6. A method for preparing the composition for improving physiological functions of livestock and poultry according to any one of claims 1 to 5, which is characterized by comprising the following operation steps:
1) Size mixing: taking the components of each active ingredient according to the parts by weight, adding sterile normal saline, and uniformly mixing to obtain composition slurry;
2) And (3) forming: the preparation method comprises the steps of molding and preparing the composition.
7. The method for preparing the composition for improving the physiological functions of livestock and poultry according to the claim 6, wherein in the step 1), the ratio of feed to liquid is 1: and adding sterile physiological saline in a proportion of 0.8-1.2.
8. The method for preparing the composition for improving the physiological functions of livestock and poultry according to claim 6, wherein the method further comprises preparing transfer factor of sheep placenta before size mixing, comprising removing fat and coating from fresh sheep placenta, crushing and grinding the fresh sheep placenta, repeatedly freezing and thawing for 3 times after crushing cells by ultrasonic cell, centrifuging to obtain supernatant, filtering, obtaining transfer factor solution below 1000 daltons by molecular interception, measuring the concentration of the transfer factor contained in the transfer factor solution, and taking the transfer factor solution according to the mass parts of the transfer factor of sheep placenta in the composition.
9. The method for preparing the composition for improving physiological functions of livestock and poultry according to claim 6, wherein the step 1) is carried out by adding sterile physiological saline, uniformly mixing at 2-8 ℃, and adjusting pH=6.5-7.5 to obtain the composition slurry.
10. The method for preparing the composition for improving the physiological function of livestock and poultry according to claim 6, wherein the forming in the step 2) comprises the step of taking the composition slurry prepared in the step 1) for freeze-drying treatment to prepare the composition freeze-dried powder for improving the physiological function of the livestock and poultry.
CN202111096924.2A 2021-09-18 2021-09-18 Composition for improving physiological functions of livestock and poultry and preparation method thereof Active CN113730552B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111096924.2A CN113730552B (en) 2021-09-18 2021-09-18 Composition for improving physiological functions of livestock and poultry and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111096924.2A CN113730552B (en) 2021-09-18 2021-09-18 Composition for improving physiological functions of livestock and poultry and preparation method thereof

Publications (2)

Publication Number Publication Date
CN113730552A CN113730552A (en) 2021-12-03
CN113730552B true CN113730552B (en) 2024-01-23

Family

ID=78739809

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111096924.2A Active CN113730552B (en) 2021-09-18 2021-09-18 Composition for improving physiological functions of livestock and poultry and preparation method thereof

Country Status (1)

Country Link
CN (1) CN113730552B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114917251A (en) * 2022-05-27 2022-08-19 福建农业职业技术学院 Preparation method of pig placenta transfer factor

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0838160A1 (en) * 1996-10-25 1998-04-29 Ajinomoto Co., Ltd. The use of poly-gamma-glutamic acid for preparing an agent for increasing the phosphorus assimilation
EP1591018A1 (en) * 2004-04-28 2005-11-02 Cybelia Feed for laying hens, method of feeding and eggs enriched with essential elements
CN105999231A (en) * 2016-06-06 2016-10-12 张英杰 Polypeptide composition, composition for improving livestock and poultry immune function, and preparation method of composition for improving livestock and poultry immune function
CN106804880A (en) * 2017-01-13 2017-06-09 山东利坤生物科技有限公司 A kind of livestock and poultry supplement calcium feed addictive and its manufacture method
CN110559421A (en) * 2019-10-09 2019-12-13 内蒙古元本生物医药科技有限公司 Medical application of placenta transfer factor and preparation method thereof
CN111388505A (en) * 2020-04-02 2020-07-10 李小波 Preparation method of animal placenta transfer factor
CN112690379A (en) * 2020-12-30 2021-04-23 仲恺农业工程学院 Preparation of zinc gluconate and application thereof in growth performance, tissue mineralization, antioxidation and immune response of freshwater fish
CN112998136A (en) * 2021-04-06 2021-06-22 北京中科万联科技有限公司 Vitamin composition for livestock and poultry as well as preparation method and application thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0838160A1 (en) * 1996-10-25 1998-04-29 Ajinomoto Co., Ltd. The use of poly-gamma-glutamic acid for preparing an agent for increasing the phosphorus assimilation
EP1591018A1 (en) * 2004-04-28 2005-11-02 Cybelia Feed for laying hens, method of feeding and eggs enriched with essential elements
CN105999231A (en) * 2016-06-06 2016-10-12 张英杰 Polypeptide composition, composition for improving livestock and poultry immune function, and preparation method of composition for improving livestock and poultry immune function
CN106804880A (en) * 2017-01-13 2017-06-09 山东利坤生物科技有限公司 A kind of livestock and poultry supplement calcium feed addictive and its manufacture method
CN110559421A (en) * 2019-10-09 2019-12-13 内蒙古元本生物医药科技有限公司 Medical application of placenta transfer factor and preparation method thereof
CN111388505A (en) * 2020-04-02 2020-07-10 李小波 Preparation method of animal placenta transfer factor
CN112690379A (en) * 2020-12-30 2021-04-23 仲恺农业工程学院 Preparation of zinc gluconate and application thereof in growth performance, tissue mineralization, antioxidation and immune response of freshwater fish
CN112998136A (en) * 2021-04-06 2021-06-22 北京中科万联科技有限公司 Vitamin composition for livestock and poultry as well as preparation method and application thereof

Also Published As

Publication number Publication date
CN113730552A (en) 2021-12-03

Similar Documents

Publication Publication Date Title
CN102481371B (en) Sticky soft gel for treating poultry
CN101828647B (en) Health care feedstuff additive for animals
CN103262943B (en) A kind of Herba Lophatheri extract and application thereof
CN105639401A (en) Feeding mold removal agent formula and application method
CN104521885A (en) Antibiotic-free broiler chicken feeding method
RU2694192C2 (en) Additive containing baicalin, in particular from scutellaria baicalensis extract, and animal fodder containing such additive
CN113730552B (en) Composition for improving physiological functions of livestock and poultry and preparation method thereof
CN102696887B (en) Feed active egg powder with nutrition, healthcare and growth promotion functions and production method thereof
CN104208677B (en) A kind of anti-newcastle disease, infectious bronchitis, egg-decreasing syndrome, the freeze-dried mixed powder and preparation method thereof of bird flu
CN102304182B (en) Preparation method and use of enterotoxigenic Escherichia coli egg yolk antibody powder
Riad et al. Effect of Spirulina platensis microalga additive on performance of growing Friesian calves
CN101366453A (en) Non-antibiotic medicament for preventing and controlling pig lax
CN108165522B (en) Embryonic-period supplement for improving quality of broiler chickens and promoting early growth and application thereof
CN102754733A (en) Novel micro-ecologic preparation for pig and preparation method thereof
CN104208678B (en) A kind of antivirus veterinary composition, lyophilized powder, preparation method and application
MXPA05001725A (en) The use of fermented wheat-germ in the feeding and veterinary practice.
CN114032200A (en) Preparation method of bacillus subtilis feed additive for breeding animals
CN106666150A (en) Nonreactive health-care agent capable of improving production performance of breeding pigs and preparation method of nonreactive health-care agent
CN103272240B (en) Composite heat protectant for specific transfer factor of nephropathogenic infectious bronchitis, and application thereof
CN107998332B (en) Traditional Chinese medicine composition for treating porcine reproductive and respiratory syndrome and application thereof
CN105661039A (en) Breeding sow feed additives and preparation method thereof
CN100450543C (en) Animal medicine capable of increasing immunologic function of livestock and birds
RU2410108C1 (en) Method to feed probiotic to suckling piglets
RU2524664C1 (en) Method of prevention of mass gastrointestinal and respiratory diseases of suckling pigs
CN106333065A (en) Traditional Chinese medicine composition for improving immunity of livestock and poultry and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant