CN104208681B - A kind of freeze-dried mixed powder of anti-duck viral disease and preparation method thereof - Google Patents
A kind of freeze-dried mixed powder of anti-duck viral disease and preparation method thereof Download PDFInfo
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- CN104208681B CN104208681B CN201310463393.5A CN201310463393A CN104208681B CN 104208681 B CN104208681 B CN 104208681B CN 201310463393 A CN201310463393 A CN 201310463393A CN 104208681 B CN104208681 B CN 104208681B
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Abstract
The invention discloses freeze-dried mixed powder of a kind of anti-duck viral disease and preparation method thereof, this freeze-dried mixed powder includes the component of following parts by weight: anti-duck virosis yolk antibody 90～95 parts, anti-duck virosis transfer factor 1～2 parts, interferon-alpha 1～2 parts, preservative 0.01～0.02 part, inactivator 0.2～0.3 part, selenium 0.05～0.1 part, antioxidant 1～2 parts, polyvinylpyrrolidone 1～2 parts, glycine 0.5～1 part, active lactobacillus 0.5～1 part.The freeze-dried mixed powder of the anti-duck viral disease of the present invention, anti-duck virosis yolk antibody, anti-duck virosis transfer factor are compounded with interferon-alpha, and it is aided with multiple auxiliary materials, each component cooperates, coordinative role, the effect of its preventing and treating duck viral disease can be significantly improved, there is advantage with strong points, that room temperature lower holding time length, antibody titer high, biological activity is good.
Description
Technical field
The invention belongs to veterinary drug technical field, be specifically related to the freeze-dried mixed powder of a kind of anti-duck viral disease, also relate to
A kind of preparation method of the freeze-dried mixed powder of anti-duck viral disease.
Background technology
Duck viral disease is acute, the one of hyperinfection disease in various degree to the duck caused by different virus
It is referred to as, including the disease such as duck pestilence, duck viral hepatitis.Duck viral disease is modal disease in a kind of duck culturing industry, by
In its have morbidity suddenly, the course of disease is short, mortality rate high, remains one of main harm of duck culturing industry at present.
The method of preventing and treating duck viral disease is more at present, mainly uses Western medicine, immunoprophylaxis, Chinese herbal medicine etc. to prevent and treat.
Wherein, spreading unchecked that Western medicine uses brings great potential safety hazard to duck meat, egg food, and drug residue has become as impact
One of principal element of duck meat, egg product safety.Immunoprophylaxis early stage results is preferable, however it is necessary that and repeatedly inoculates, operation fiber crops
Tired, relatively costly, easily cause stress；And vaccine requires higher for storage conditions, need 2-8 DEG C of condition
Lower cold preservation, room temperature condition lower a period of time then loses effect.Chinese herbal feed additive is also one of current research direction,
But owing to the kind of Chinese herbal medicine is more, identical Chinese crude drug is very big to the difference between the effects of different diseases of bird and livestock, and Chinese herbal medicine
Extremely limited to the immune effect of disease, therefore apply and also there is certain limitation.
Summary of the invention
It is an object of the invention to provide the freeze-dried mixed powder of a kind of anti-duck viral disease, solve prior art preventing and treating duck viral
The problem that the operation that disease exists is inconvenient, medicament is difficult to preserve, effect is poor.
Second object of the present invention is to provide the preparation method of the freeze-dried mixed powder of a kind of anti-duck viral disease.
In order to realize object above, the technical solution adopted in the present invention is: the freeze-dried mixed powder of a kind of anti-duck viral disease,
Component including following parts by weight: anti-duck virosis yolk antibody 90～95 parts, anti-duck virosis transfer factor 1～2 parts,
Alpha-interferon 1～2 parts, preservative 0.01～0.02 part, inactivator 0.2～0.3 part, selenium 0.05～0.1 part, antioxidant
1～2 part, polyvinylpyrrolidone 1～2 parts, glycine 0.5～1 part, active lactobacillus 0.5～1 part.
Described inactivator is formaldehyde.
Described preservative is thimerosal.
Described antioxidant is any one or a combination thereof in vitamin C, vitamin E.
The preparation method of the freeze-dried mixed powder of a kind of above-mentioned anti-duck viral disease, comprises the following steps:
A. using duck viral epidemic disease Virus Standard strain to prepare vaccine, immune health duck group obtains high-immunity egg, is passed through by high-immunity egg
Sterilization, acidification, centrifugal treating obtain the supernatant containing anti-duck virosis yolk antibody；
B. by the supernatant containing anti-duck virosis yolk antibody and anti-duck virosis transfer factor, preservative, inactivator, selenium,
Antioxidant, alpha-interferon, active lactobacillus, polyvinylpyrrolidone, glycine mix, then lyophilizing and get final product.
Preferably, the preparation method of the freeze-dried mixed powder of above-mentioned anti-duck viral disease, comprise the following steps:
1) take duck viral epidemic disease Virus Standard strain to breed, inactivated and be prepared as vaccine, at interval of 7 days to health
Laying ducks group carries out immunity, and altogether immunity three times, start after immunity to detect antibody titer for the second time, when antibody titer reaches 1:
When 256, collect high-immunity egg, carry out the high-immunity egg of collection clearing up, sterilizing, dry standby；
2) by step 1) gained high-immunity egg isolated high-immunity egg yolk, add isopyknic sterilized water, be sufficiently stirred for,
To yolk antibody solution, yolk antibody solution adds acidified aqueous solution, mixes, staticly settle, take supernatant standby；
3) in step 2) gained supernatant adds sodium salt make sodium salt volume of the total volume 0.8%, centrifugation, must contain
There is the supernatant of anti-duck virosis yolk antibody；
4) step 3 is taken) supernatant of the anti-duck virosis yolk antibody containing 90～95 weight portions of gained, add 1～2
Weight portion anti-duck virosis transfer factor, filters, and reserved filtrate is standby；
5) by step 4) gained filtrate is concentrated into 2.5ml/500 plumage part, adds preservative 0.01～0.02 weight portion, inactivation
Agent 0.2～0.3 weight portion, selenium 0.05～0.1 weight portion, antioxidant 1～2 weight portion, alpha-interferon 1～2 weight portion,
Active lactobacillus 0.5～1 weight portion, polyvinylpyrrolidone 1～2 weight portion, glycine 0.5～1 weight portion mix
Close, first-20 DEG C of pre-coolings, then lyophilizing under the conditions of-80 DEG C, to obtain final product.
Step 2) described in the pH of acidified aqueous solution be 4.9～5.2；The addition of acidified aqueous solution is yolk antibody solution body
Long-pending 8 times.Described acidified aqueous solution can be acidified aqueous solution.
Step 3) described in sodium salt be NaCl and Na2SO4The mixture that mass ratio is 4:1.
Step 3) described in the rotating speed of centrifugation be 15000rpm, the time is 20min.
Step 4) described in filter be first to filter with the filter membrane of 0.45 μm, molecular cut off dividing greatly more than 200KD
Son, then by filtrate with the membrane filtration of 0.22 μm.
Yolk antibody is a kind of immunoglobulin, the one produced by the stimulation of external antigen due to body stress product,
Can combine with corresponding antigens, eliminate the cause of disease.The present invention, can by duck group healthy for the vaccine virus immunization of duck viral disease
So that the Ovum Anas domestica egg yolk obtained produces effective anti-duck viral yolk antibody, decrease the probability of strain reversion simultaneously.
Transfer factor carries the specific immunity information of primed lymphocyte, it is possible to specific immunity information is presented to receptor
Lymphocyte, the lymphocyte making receptor inactive is changed into specificity primed lymphocyte, thus excites recipient cell to mediate
Immunoreation.
Alpha-interferon makes cell produce antiviral protein by cell surface receptor effect, the most also can strengthen natural killer cell
(NK cell), macrophage and the vigor of T lymphocyte, thus play immunoregulation effect, and strengthen antiviral energy
Power.
In the freeze-dried mixed powder of the anti-duck viral disease of the present invention, anti-duck virosis yolk antibody is a kind of immunoglobulin,
Duck viral disease is had good prevention effect；Anti-duck virosis transfer factor itself is a kind of special antiviral agent；α-
Above-mentioned substance can be combined make with the function of immune system of enhancing body by interferon, active lactobacillus as immunostimulant
With, prevention and the effect for the treatment of duck viral disease can be greatly enhanced.
The freeze-dried mixed powder of the anti-duck viral disease of the present invention, by anti-duck virosis yolk antibody, anti-duck virosis transfer because of
Son compounds with immunostimulant (alpha-interferon, active lactobacillus), and is aided with antioxidant, preservative, inactivation
Freeze-dried mixed powder prepared by agent, selenium, polyvinylpyrrolidone and glycine, and each component cooperates, coordinative role, Ke Yixian
Write the effect improving its preventing and treating duck viral disease, there is with strong points, room temperature lower holding time length, antibody titer high, raw
The advantage that thing activity is good, is a kind of potent biological preparation with wide application prospect；Prove through animal experiment, the present invention
The freeze-dried mixed powder of anti-duck viral disease, the prevention effect of duck viral disease to be substantially better than anti-duck virosis yolk
The situation that in antibody, anti-duck virosis transfer factor, alpha-interferon, any one is used alone or two of which is applied in combination.
The freeze-dried mixed powder of the anti-duck viral disease of the present invention, during use, can as feed additive press mass fraction 2%～
3% is mixed in feedstuff；Intramuscular injection mode is used to be administered during morbidity, containing freeze-dried mixed powder 1g in every 100ml injection, injection
The usage amount of liquid is each 0.1～0.2ml/kg body weight；Medication first doubles, and therapeutic effect is more preferable.
The preparation method of the freeze-dried mixed powder of the anti-duck viral disease of the present invention, the most anti-duck virosis yolk antibody, anti-duck
Virosis transfer factor, immunostimulant (alpha-interferon, active lactobacillus) and multiple auxiliary materials compound, and pass through
Adjust the composition of adjuvant, control its consumption, extend the holding time of yolk antibody further, it is ensured that yolk antibody is often
Activity in the temperature lower long period, reduces the storage and transport cost of freeze-dried powder preparation, easy to use, widely should have
Use prospect；Preparation method technique is simple, easy to operate, greatly reduces production cost, is suitable for large-scale industrial production.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further illustrated.
In the detailed description of the invention of the present invention, duck virus stain used is national standard strain；Alpha-interferon used is purchased from Changchun
Institute of biological products.
In the detailed description of the invention of the present invention, anti-duck virosis transfer factor used, following methods prepare:
1), after pig spleen and/or thymus being carried out immunity with duck viral epidemic disease standard virus antigen (commercially available), its surface is gone to
Muscle, fat, then with the normal saline flushing after sterilizing；
2) pig spleen after rinsing and/or thymus broken after, add the PBS after the pre-cooling of 2 times of volumes, use group
Knit Syrup-homogenizing instrument it is homogeneous, obtain homogenate；
3), after homogenate being processed 5～10min with Ultrasonic Cell Disruptor, regulation pH is 5.5, at 5000～6000rpm rotating speeds
Under be centrifuged 30min, collect supernatant；
4) by step 3) gained supernatant micro-filtrate membrane filtration, molecular cut off is the material of more than 7000 dalton, will
Gained filtrate uses degerming membrane filtration, obtains anti-duck virosis transfer factor stock solution, saves backup under the conditions of 4 DEG C.
Embodiment 1
The freeze-dried mixed powder of the anti-duck viral disease of the present embodiment is the freeze-dried mixed powder of anti-duck pestilence, including following parts by weight
Component: anti-duck plague yolk antibody 90 parts, anti-duck plague transfer factor 2 parts, alpha-interferon 2 parts, active lactobacillus 1
Part, thimerosal 0.02 part, 0.2 part of formaldehyde, 0.05 part of selenium, vitamin C 1 part, vitamin E 1 part, polyvinyl pyrrole
Alkanone 2 parts, glycine 0.5 part.
The preparation method of the freeze-dried mixed powder of the anti-duck pestilence of the present embodiment, comprises the following steps:
1) take duck plague virus standard strain to breed, inactivated and be prepared as vaccine, at interval of 7 days to healthy laying ducks
Group carries out immunity, altogether immunity three times, and immune health duck group inoculates inactivated vaccine 1.5ml for the first time, for the second time, for the third time
Immunity inoculation dosage 1 times, starts after immunity to detect antibody titer for the second time, when antibody titer reaches 1:256, collects
High-immunity egg, carries out the high-immunity egg of collection clearing up, sterilizing, dries standby；
2) by step 1) gained high-immunity egg isolated high-immunity egg yolk, add isopyknic sterilized water, be sufficiently stirred for,
To yolk antibody solution, adding pH in yolk antibody solution is the acidified aqueous solution of 5.0, and acidified aqueous solution addition is ovum
8 times of yellow antibody-solutions volume, mix, staticly settle, take supernatant standby；
3) in step 2) gained supernatant adds by NaCl and Na2SO4Ratio for 4:1 in mass ratio is mixed to form
Mixing sodium salt, makes mixing sodium salt volume of the total volume 0.8%, is centrifuged 20min with the rotating speed of 15000rpm, must be containing anti-
The supernatant of duck plague yolk antibody；
4) step 3 is taken) supernatant of the anti-duck plague yolk antibody containing 90 weight portions of gained, add the 2 anti-ducks of weight portion
Pestilence transfer factor, filters with the filter membrane of 0.45 μm, the molecular weight that the dams macromolecular substances more than 200KD, then will filter
Liquid is through 0.22 μm membrane filtration, and reserved filtrate is standby；
5) by step 4) gained filtrate is concentrated into 2.5ml/500 plumage part, adds thimerosal 0.02 weight portion, formaldehyde 0.2 weight
Amount part, selenium 0.05 weight portion, vitamin C 1 weight portion, vitamin E 1 weight portion, alpha-interferon 2 weight portion, activity
Lactobacillus 1 weight portion, polyvinylpyrrolidone 2 weight portion, glycine 0.5 weight portion mix, first-20 DEG C of pre-coolings,
Lyophilizing under the conditions of-80 DEG C again, to obtain final product.
Embodiment 2
The freeze-dried mixed powder of the anti-duck viral disease of the present embodiment is the freeze-dried mixed powder of anti-duck viral hepatitis, including following
The component of parts by weight: anti-duck virus hepatitis yolk antibody 93 parts, anti-duck viral hepatitis transfer factor 1.5 parts, α-dry
Disturb element 1.5 parts, active lactobacillus 1 part, thimerosal 0.01 part, 0.3 part of formaldehyde, 0.1 part of selenium, vitamin C 0.5 part,
Vitamin E 0.5 part, polyvinylpyrrolidone 1.5 parts, glycine 1 part.
The preparation method of the freeze-dried mixed powder of the anti-duck viral hepatitis of the present embodiment, comprises the following steps:
1) take virulent duck enteritis virus standard strain to breed, inactivated and be prepared as vaccine, at interval of 7 days to strong
Health laying ducks group carries out immunity, altogether immunity three times, and immune health duck group inoculates inactivated vaccine 1.5ml for the first time, for the second time,
Immunity inoculation dosage 1 times for the third time, starts after immunity to detect antibody titer for the second time, when antibody titer reaches 1:256,
Collect high-immunity egg, carry out the high-immunity egg of collection clearing up, sterilizing, dry standby；
2) by step 1) gained high-immunity egg isolated high-immunity egg yolk, add isopyknic sterilized water, be sufficiently stirred for,
To yolk antibody solution, adding pH in yolk antibody solution is the acidified aqueous solution of 5.0, and acidified aqueous solution addition is ovum
8 times of yellow antibody-solutions volume, mix, staticly settle, take supernatant standby；
3) in step 2) gained supernatant adds by NaCl and Na2SO4Ratio for 4:1 in mass ratio is mixed to form
Mixing sodium salt, makes mixing sodium salt volume of the total volume 0.8%, is centrifuged 20min with the rotating speed of 15000rpm, must be containing anti-
The supernatant of duck virus hepatitis yolk antibody；
4) step 3 is taken) supernatant of the anti-duck virus hepatitis yolk antibody containing 93 weight portions of gained, add 1.5
The anti-duck viral hepatitis transfer factor of weight portion, filters with the filter membrane of 0.45 μm, and the molecular weight that dams is more than 200KD's
Macromolecular substances, then by filtrate through 0.22 μm membrane filtration, reserved filtrate is standby；
5) by step 4) gained filtrate is concentrated into 2.5ml/500 plumage part, adds thimerosal 0.01 weight portion, formaldehyde 0.3 weight
Amount part, selenium 0.1 weight portion, vitamin C 0.5 weight portion, vitamin E 0.5 weight portion, alpha-interferon 1.5 weight portion,
Active lactobacillus 1 weight portion, polyvinylpyrrolidone 1.5 weight portion, glycine 1 weight portion mix, first-20 DEG C
Pre-cooling, then lyophilizing under the conditions of-80 DEG C, to obtain final product.
Experimental example 1
This experimental example carries out performance detection to the embodiment 1～2 freeze-dried mixed powder of gained, and result is as shown in table 1,2.
Room temperature bioactivity experimental technique: the embodiment 1～2 freeze-dried mixed powder preparation of gained is saved in 37 DEG C of incubators,
Preserve 24 months, respectively 1 week, 2 weeks, January, February, April, August, December, 16 months, 20 months, after 24 months,
By agar diffusion (AGP) method, measure the yolk antibody titer change of the taken sample of each time point.
Table 1 embodiment 1～2 gained freeze-dried mixed powder room temperature bioactivity experimental result
| Group | 1 week | 2 weeks | January | February | April | August | December | 16 months | 20 months | 24 months |
| Embodiment 1 | 1:32 | 1:32 | 1:32 | 1:32 | 1:32 | 1:16 | 1；16 | 1:16 | 1:16 | 1:16 |
| Embodiment 2 | 1:32 | 1:32 | 1:32 | 1:32 | 1:32 | 1:32 | 1；16 | 1:16 | 1:16 | 1:16 |
Table 2 embodiment 1～2 gained freeze-dried mixed powder room temperature places performance test results
Experimental example 2
The effect that the freeze-dried mixed powder of the anti-duck pestilence of embodiment 1 is prevented and treated duck pestilence by this experimental example carries out clinical experiment.
Experimental technique: choosing 1 monthly age duck 900, wherein 100 be only used as counteracting toxic substances group isolated rearing, 100 are only used as blank
Group isolated rearing, 600 are only used as matched group is divided into 6 groups, often group 100, isolated rearing, respectively by comparative example 1～6
Preparation for treating, other 100 are only used as test group, with the preparation for treating of embodiment 1.Feeding manner is that free choice feeding is commonly raised
Material, freely drinks water, and after 24h, takes the duck plague virus liquid after the duck intramuscular injection inoculation dilution of test group, matched group and counteracting toxic substances group,
Inoculum concentration is 2ml/；Beginning for property treatment after infection, intramuscular injection, injection dosage is 0.2ml/kg body weight；Note
Death condition after record 120h, result is as shown in table 3.
The table 3 embodiment 1 freeze-dried mixed powder of the gained prevention effect experimental result to duck pestilence
Wherein, the reagent combination of comparative example 1～6 is as shown in table 4.
The reagent combination of table 4 comparative example 1～6
It can be seen that the freeze-dried mixed powder of embodiment 1 gained can effectively prevent and treat duck pestilence, and freeze-dried mixed powder from table 3,4
Effect to be substantially better than in anti-duck plague yolk antibody, anti-duck plague transfer factor, alpha-interferon any one and be used alone or wherein
Two kinds of situations about being applied in combination.
Experimental example 3
The effect that the freeze-dried mixed powder of the anti-duck viral hepatitis of embodiment 2 is prevented and treated duck viral hepatitis by this experimental example is faced
Bed experiment.
Experimental technique: choosing 1 monthly age duck 900, wherein 100 be only used as counteracting toxic substances group isolated rearing, 100 are only used as blank
Group isolated rearing, 600 are only used as matched group is divided into 6 groups, often group 100, isolated rearing, respectively by comparative example 7～12
Preparation for treating, other 100 are only used as test group, with the preparation for treating of embodiment 2.Feeding manner is that free choice feeding is common
Feedstuff, freely drinks water, and after 24h, takes the duck viral liver after the duck intramuscular injection inoculation dilution of test group, matched group and counteracting toxic substances group
Scorching virus liquid, inoculum concentration is 2ml/；Beginning for property treatment after infection, intramuscular injection, injection dosage is 0.2ml/kg
Body weight；Death condition after record 120h, result is as shown in table 5.
The table 5 embodiment 2 freeze-dried mixed powder of the gained prevention effect experimental result to duck viral hepatitis
Wherein, the reagent combination of comparative example 7～12 is as shown in table 6.
The reagent combination of table 6 comparative example 7～12
It can be seen that the freeze-dried mixed powder of embodiment 2 gained can effectively prevent and treat duck viral hepatitis, and mixing from table 5,6
Lyophilized powder effect to be substantially better than in anti-duck virus hepatitis yolk antibody, anti-duck viral hepatitis transfer factor, alpha-interferon
The situation that any one is used alone or two of which is applied in combination.
Claims (9)
1. the freeze-dried mixed powder of an anti-duck viral disease, it is characterised in that: include the component of following parts by weight: anti-
Duck virosis yolk antibody 90～95 parts, anti-duck virosis transfer factor 1～2 parts, alpha-interferon 1～2 parts, preservative
0.01～0.02 part, inactivator 0.2～0.3 part, selenium 0.05～0.1 part, antioxidant 1～2 parts, polyvinylpyrrolidone
1～2 part, glycine 0.5～1 part, active lactobacillus 0.5～1 part；Described antioxidant is vitamin C, vitamin E
In any one or a combination thereof.
The freeze-dried mixed powder of anti-duck viral disease the most according to claim 1, it is characterised in that: described inactivator
For formaldehyde.
The freeze-dried mixed powder of anti-duck viral disease the most according to claim 1, it is characterised in that: described preservative
For thimerosal.
4. the preparation method of the freeze-dried mixed powder of an anti-duck viral disease as claimed in claim 1, it is characterised in that:
Comprise the following steps:
A. using duck viral epidemic disease Virus Standard strain to prepare vaccine, immune health duck group obtains high-immunity egg, is passed through by high-immunity egg
Sterilization, acidification, centrifugal treating obtain the supernatant containing anti-duck virosis yolk antibody；
B. by the supernatant containing anti-duck virosis yolk antibody and anti-duck virosis transfer factor, preservative, inactivator, selenium,
Antioxidant, alpha-interferon, active lactobacillus, polyvinylpyrrolidone, glycine mix, then lyophilizing and get final product.
The preparation method of the freeze-dried mixed powder of anti-duck viral disease the most according to claim 4, it is characterised in that:
Comprise the following steps:
1) take duck viral epidemic disease Virus Standard strain to breed, inactivated and be prepared as vaccine, at interval of 7 days to health
Laying ducks group carries out immunity, and altogether immunity three times, start after immunity to detect antibody titer for the second time, when antibody titer reaches 1:
When 256, collect high-immunity egg, carry out the high-immunity egg of collection clearing up, sterilizing, dry standby；
2) by step 1) gained high-immunity egg isolated high-immunity egg yolk, add isopyknic sterilized water, be sufficiently stirred for,
To yolk antibody solution, yolk antibody solution adds acidified aqueous solution, mixes, staticly settle, take supernatant standby；
3) in step 2) gained supernatant adds sodium salt make sodium salt volume of the total volume 0.8%, centrifugation, must contain
There is the supernatant of anti-duck virosis yolk antibody；
4) step 3 is taken) supernatant of the anti-duck virosis yolk antibody containing 90～95 weight portions of gained, add 1～2
Weight portion anti-duck virosis transfer factor, filters, and reserved filtrate is standby；
5) by step 4) gained filtrate is concentrated into 2.5ml/500 plumage part, adds preservative 0.01～0.02 weight portion, inactivation
Agent 0.2～0.3 weight portion, selenium 0.05～0.1 weight portion, antioxidant 1～2 weight portion, alpha-interferon 1～2 weight portion,
Active lactobacillus 0.5～1 weight portion, polyvinylpyrrolidone 1～2 weight portion, glycine 0.5～1 weight portion mix
Close, first-20 DEG C of pre-coolings, then lyophilizing under the conditions of-80 DEG C, to obtain final product.
The preparation method of the freeze-dried mixed powder of anti-duck viral disease the most according to claim 5, it is characterised in that:
Step 2) described in the pH of acidified aqueous solution be 4.9～5.2；The addition of acidified aqueous solution is yolk antibody liquor capacity
8 times.
The preparation method of the freeze-dried mixed powder of anti-duck viral disease the most according to claim 5, it is characterised in that:
Step 3) described in sodium salt be NaCl and Na2SO4The mixture that mass ratio is 4:1.
The preparation method of the freeze-dried mixed powder of anti-duck viral disease the most according to claim 5, it is characterised in that:
Step 3) described in the rotating speed of centrifugation be 15000rpm, the time is 20min.
The preparation method of the freeze-dried mixed powder of anti-duck viral disease the most according to claim 5, it is characterised in that:
Step 4) described in filter be first to filter with the filter membrane of 0.45 μm, molecular cut off be more than 200KD macromole,
Again by filtrate with the membrane filtration of 0.22 μm.
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| CN101293915A (en) * | 2008-06-17 | 2008-10-29 | 天津生机集团股份有限公司 | Method for preparing anti-duck plague transfer factor |
| CN102716483A (en) * | 2012-05-31 | 2012-10-10 | 郑州后羿制药有限公司 | Duck plague yolk antibody freeze-dried powder and preparation method thereof |
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