RU2131673C1 - Method of preparing ozonized oil "otrisan" - Google Patents

Abstract

FIELD: biotechnology and medicine. SUBSTANCE: vegetal oil is affected by ozone-containing gas, e.g. oxygen-ozone mixture, bubbled through oil in low-frequency ultrasound zone, while ozone-containing gas is directionally pulverized into zone of developed cavitation of ultrasonically affected oil. Such operation mode increases degree of saturation of oil with ozone and simultaneously provides metastable structure of oil. EFFECT: increased biological activity and durability of antiinflammatory, bactericidal, virulicidal, fungicidal, and detoxification activities. 2 cl, 5 dwg, 5 ex

Description

 The invention relates to biotechnology and medicine, in particular to the production of oil-based ozonized substances with high biological activity and long-term preservation of its anti-inflammatory, bactericidal, virucidal, fungicidal and detoxification activity.

 A known method of producing ozonized olive oil with a pronounced bactericidal, antiviral and fungicidal effect, which consists in the fact that before saturation with ozone, the following ingredients are introduced into it: 5.6% oily, 0.2% linoleic, 11.7% palmitic and 2, 7% stearic acid, as well as 8.3% unsaturated fatty acids with a relatively high peroxide number P = 349, which is a measure of the content of active oxygen bound in peroxide form (the peroxide value of pure olive oil is t P = 19,2).

 However, due to the unstable structure of ozonized oil and the rapid process of its decomposition, it was not widely used (see Vibran R. // Materials of the symposium. "Congress of ozone therapists. - Vienna, 1980. - P. 76; Landauer V. // Materials of the symposium on ozone therapy. - Vienna, 1980. - S. 76; Landauer V. // Materials of the symposium on ozone therapy. - Baden-Baden, 1981. - S. 93 and others).

A known method for producing ozonized vegetable oil (olive, linseed, sunflower, almond, castor, etc.) by saturating the oil with an ozone-containing gas component, for example, an oxygen-ozone mixture, while it is bubbled through a layer of ozonized vegetable oil (see RF Patent N 2040235 IPC A 61 F 11/00, 1995)
This method of producing ozonized vegetable oil can increase the peroxide number for ozonides to a value of P = 700 - 900 immediately after saturation of the oil with ozone. This value of the peroxide number P is most effective for the prevention and treatment of foci of infection of various etiologies and localizations.

 However, over time, the degree of saturation of vegetable oil with ozone sharply decreases to a value of P = 200 and lower due to its decomposition, degassing, etc. Moreover, the therapeutic effectiveness of ozonized oil drops sharply. This requires a constant and repeated resumption of the process of obtaining ozonized oil with a large peroxide number. The method also does not provide, without the involvement of biologically active components, modifiers, to achieve a stable and stable structure of the resulting ozonized vegetable oil, ensuring the preservation for a long time (3-6 months) of the peroxide value at the required high level.

 The objective of the invention is to increase the efficiency of the ozonation process when the metastable structure of the resulting ozonized vegetable oil is achieved, which for a long time keeps the value of the peroxide number at the required high level.

 The problem is achieved in that in the known method for producing ozonized vegetable oil by sparging oil with an ozone-containing gas component, it is additionally exposed to low-frequency ultrasound (18 - 44 kHz), while the ozone-containing gas component is directedly sprayed into the developed cavitation area of the voiced oil volume. As the ozone-containing gas component, for example, an oxygen-ozone mixture is used.

 The technical result from the use of the method is to increase the degree of saturation of the oil with ozone while achieving a metastable structure of the resulting oil, which provides the latter with high biological activity that persists for a long time.

The invention is illustrated by drawings, which depict:
- in FIG. 1 - block diagram of the installation for implementing the method of producing ozonized oil "OTRISAN";
- in FIG. 2. - ultrasonic (working) camera (in section);
- in FIG. 3. - ultrasonic (working) camera (section along AA);
- in FIG. 4. - the achievable value of the peroxide number P for various methods of saturation of oil with ozone;
- in FIG. 5. - the dynamics of the reduction of the peroxide number P depending on the method of its ozonation and its storage time.

 The OTRISAN installation for producing ozonized oil contains the following main blocks and elements (Fig. 1.): a power supply unit (1), an ozone generator (GO) block (2) with a cleaner and dehumidifier, pumped through the GO discharge electrode system (on not shown); ultrasonic generator (UZG) (3); ultrasonic (working) camera (ultrasound camera) (4); excess ozone deactivator (5); distribution valve (6); outlet pneumatic line (7); supply air line (8); stopcock (9); hydraulic line (10).

 In turn, the ultrasonic camera (4) (Fig. 2. and Fig. 3.) contains a supporting body (4.1) with a removable cover (4.2), forming a sealed container (4.3). A piezoceramic ultrasonic transducer (4.4), coupled to a disk-shaped activator waveguide (4.5), is placed on the bottom of the supporting body (4.1). A nozzle (4.6) is brought into the tank (4.3) for supplying the ozone-containing gas component to the ultrasonic chamber (4) from the GO (2) through the outlet pneumatic pipe (7), the distribution valve (6) and the pneumatic pipe (8) connected to the annular injector -spray (4.7) mounted coaxially to the activator waveguide (4.5). For directed bubbling of the sprayed ozone-containing gas component stream into the developed cavitation region created in the ultrasound field by the waveguide-activator (4.5) at a distance of the order of 1/4 - 1/2 of the wavelength from its radiating end, the injector-sprayer (4.7) is equipped with radially located nozzle holes (4.8), which are directed at an angle of 45 towards the waveguide-activator (4.5). A discharge nozzle (4.9) with an opening (4.10) is connected to the bottom of the tank (4.3), which communicates the tank through an overhead tap (9) with a hydraulic conduit (10). An excess ozone deactivator (5) is connected to a removable cover (4.2), which communicates with a sealed container (4.3), filled with a sorbing and decomposing residual, excess ozone catalyst.

The device operates as follows:
The working chamber (4) is filled with refined vegetable oil, such as olive oil, and sealed. They include a power supply unit (1) and a power supply unit (2), which through an outlet pneumatic pipe (7), a distribution valve (6), a supply pneumatic pipe (8), a pipe (4.6) and an injector-sprayer (4.7) feed into the ultrasonic chamber ( 4) an ozone-containing gas component generated from a previously purified and dried gas-air mixture. After that, include ultrasonic testing (3) and carry out the scoring of the entire volume of oil. At the same time, in the developed cavitation region, which is formed at a distance of 1/4 - 1/2 of the ultrasonic wavelength from the emitting end of the activator waveguide (4.5), an ozone-containing spray nozzle (4.8) located radially and at an angle of 45 is supplied with an ozone-containing injector-sprayer (4.8) gas component.

 To obtain a long-lasting homogeneous metastable structure of OTRISAN ozonized oil, which has a high peroxide number (of the order of P = 1200 and higher), a complex of simultaneously occurring physical and physicochemical processes is intensified in the field of low-frequency ultrasound initiated in the oil volume: disintegration, dissolution, aeration, etc. of the oil solution by sparging through it an ozone-containing gas component to obtain an ozone-containing gas-liquid emulsion.

 The resulting pronounced secondary ultrasound factors - cavitation, acoustic flows, variable sound pressure, etc. - generate in the region of the emitting end of the activator waveguide (4.5) a whole field of oscillating cavitation bubbles interacting with bubbles of an ozone-containing gas component that is sprayed and supplied to the developed cavitation area. This ensures their destruction into smaller nuclei by collapsing cavitation bubbles with the creation of a developed phase contact surface (gas-liquid). In addition, intense acoustic flows, hydrodynamic disturbances, and cumulative jets at the macro and micro levels lead to intensification of the macro and micro mixing processes, which significantly reduce the thickness of the boundary diffusion layer at the phase boundary. This leads to an acceleration of external diffusion, a decrease in the concentration gradients of dissolved substances at the phase boundary, and an acceleration of heterogeneous chemical reactions that determine the dynamics of absorption of free ozone-containing gas in the sounded liquid medium, i.e. to accelerate the process of acoustic aeration of liquid medium with ozone, with the achievement of a metastable structure.

 Unreacted ozone is evacuated from the ultrasound chamber (4) through the decontaminant of excess ozone (5), which communicates with the atmosphere in which it is sorbed and catalytically decomposed.

 After the ozonation process is completed, according to the proposed method, and the power supply unit (1), GO (2), and UZG (3) are turned off, the OTRISAN ozonized oil is supplied to the consumer via the outlet fitting (4.9).

Studies have been conducted on the acoustic aeration of ozone in vegetable oils and the optimal modes for the implementation of the OTRISAN ozonized oil production method have been identified:
- the frequency of ultrasonic vibrations is 18 - 44 kHz (the most effective area of low-frequency ultrasound, providing the severity of cavitation and other secondary processes in the voiced media),
- the amplitude of the ultrasonic vibrations of the emitting end of the waveguide-activator is 30 - 40 microns. (provided by the design capabilities of the speaker system),
- exposure of ultrasonic exposure - 1-2 sec / cm ^{3 of the} sounded oil (when the sound of oil is exposed to less than 1 sec / cm ³ , the peroxide value decreases, and with an increase in exposure over 2 sec / cm ³ the coagulation process also appears, which also reduces the value of the peroxide number ),
- the consumption of ozone-containing gas component is not less than 500 ml. / min. per 1 dm ^{3 of the} volume of the ultrasound camera,
- the concentration of ozone in the ozone-containing gas component is not less than 5 mg / l.

Example 1. As a vegetable oil was taken olive. Ozonation mode parameters:
- frequency of ultrasonic vibrations - 18 kHz
- the amplitude of the ultrasonic vibrations of the emitting end of the waveguide-activator is 30 μm,
- exposure of ultrasonic exposure - 1 sec / cm ³ sounded oil
- the flow rate of the oxygen-ozone mixture is 500 ml / min per 1 dm ^{3 of the} volume of the ultrasound camera,
- ozone concentration in the mixture - 5 mg / l
The value of the peroxide number P = 1190
Example 2. As a vegetable oil was taken sunflower. Ozonation mode parameters:
- frequency of ultrasonic vibrations - 44 kHz,
- the amplitude of the ultrasonic vibrations of the emitting end of the waveguide-activator is 40 μm,
- exposure of ultrasonic exposure - 2 sec / cm ³ sounded oil,
- the flow rate of the oxygen-ozone mixture is 700 ml / min per 1 dm ^{3 of the} volume of the ultrasound camera,
- the concentration of ozone in the mixture is 7 mg / l.

The value of the peroxide number P = 1215
Thus, it has been shown (Fig. 4) that sparging of vegetable oil with an ozone-containing gas component in the field of low-frequency ultrasound (18–44 kHz) makes it possible to increase the efficiency of its ozonation process by an average of 1.5–2 times. This is due to the fact that when bubbling an oil solution with an ozone-containing gas, and especially under cavitation conditions, the newly formed developed phase boundary (gas-liquid) acquires a huge number of uncompensated molecular bonds capable of attaching ozone or its derivatives to form ozone (ozonide) - or oxygen-containing associates absorbed by droplets (micelles) of an ozone-containing gas-liquid emulsion with a large peroxide number of the order of P = 1200 and higher, which is what determines the high anti-inflammatory, bacter tsidnye, virucidal, fungicidal and detoxification properties obtained by the inventive method ozonized oils "OTRISAN".

The metastability of the structure of the obtained ozonated oil is confirmed by data on the dynamics of a decrease in its peroxide number over 6 months when the product is stored in dark glass bottles with sealed caps at a temperature of no higher than 10 - 12 ^o C and a depressurization frequency of once every 2-3 days for a while about 1 minute (Fig. 5). The results obtained indicate that the decrease in the peroxide value under the above conditions does not exceed 10% of the initially achieved value.

High biological activity and long-lasting anti-inflammatory, bactericidal virucidal, fungicidal and detoxification activities of the OTRISAN ozonized oil obtained during the above experiment are confirmed by its clinical use in the treatment of patients (over 300 patients) with sluggish and non-healing ulcers, etc. the surgical department of the Omsk City Clinical Hospital No. 1, as well as the department of wounds and wound complications of the 3rd Central Military Hospital named after A.A. Vishnevsky:
Example 1. Patient V., 51 years old. Diagnosis: often recurring erysipelas of the left lower leg and foot, erythematous-bullous form.

 Since the applied traditional methods of treatment did not reduce the recurrence of erysipelas, then in the course of treatment they used OTRISAN ozonated olive oil, obtained according to the method described above in the description of the invention, with a peroxide value of P = 1100 - 1200.

 At the first stage of treatment, the primary focus of infection in the area of the bullae was opened by ultrasonic debridement through an intermediate solution of furatsilin 1: 5000.

 Then, in the second stage, a gauze towel soaked in OTRISAN ozonized oil was applied to the sanitized affected area of the skin, which was removed the next day with a fresh one. This stage was carried out daily until the onset of regional epithelization, noted already 3-4 days after the start of treatment. After this, only the stage of application of ozonized oil to the infection site was carried out. On day 12, there was complete epithelization of the affected area of the skin with residual effects in the form of peeling and skin pigmentation. Treatment with OTRISAN ozonized oil was discontinued. Over the next 6 months, relapse of erysipelas in the patient was not observed.

 Example 2. Patient F., 58 years old. Diagnosis: long-term non-healing ulcer of the right leg against diabetes mellitus.

 The patient suffers from diabetes for 8 years. On examination, it was found: on the medial surface of the lower third of the right lower leg, a trophic ulcer of 6 x 4.5 x 0.5 cm in size was determined. The bottom of the ulcer is filled with necrotic masses and plaque of fibrin. Previously, during the year, under the supervision of an endocrinologist, treatment was carried out using generally accepted methods (gauze dressings soaked with medicinal solutions, combined with physiotherapy, which had no effect on the healing of trophic ulcers).

 The treatment of a trophic ulcer with the help of the OTRISAN ozonated refined oil, assigned according to the method described above in the description of the invention, has begun, with the peroxide value P = 1220 - 1230.

 At the first stage of treatment, the wound surface was treated first with a 3% hydrogen peroxide solution, and then with an 1: 5000 aqueous solution of furacilin.

 At the second stage of treatment, after drying the wound surface, OTRISAN ozonized oils are applied to the ulcer and surrounding healthy tissues, covered with a gauze pad soaked with OTRISAN ozonized oil, and then a bandage is applied. During the checkup of the patient's condition after 3 months, no ulcer recurrence was detected.

 Example 3. Patient D., 43 years old. Diagnosis: vulvar kraurosis. The duration of the disease is 2 years after the operation to remove the right appendages. Over the past 1.5 years, dryness and severe itching in the perineum, cracks in the area of the posterior commissure and anus have been noted.

 The previously applied local treatment with fat tampons and harmonious ointments (lorenden, prednisone, hydrocortisone) allowed to obtain only a short-term effect. It was proposed and carried out the treatment using the OTRISAN ozonated refined sunflower oil obtained according to the method described above in the invention with a peroxide value of P = 1220 - 1230. The treatment was carried out in a hospital in the evening to ensure long-term contact of the ozonized oil with the affected area for night sleep time.

 At the first stage, ultrasonic sanitation of the perineum was carried out through an intermediate antiseptic solution (3% hydrogen peroxide solution or 1: 5000 furatsilin solution). As a second step, a 2-3 layer gauze pad saturated with ozonized oil was applied to the affected area and adjacent healthy tissues. Treatment was carried out daily for 10 sessions.

 After the 3rd session, the patient noted the subsidence of itching, normalization of sleep, improvement in general condition. After the 5th and 6th sessions, a decrease in inflammatory phenomena and a restoration of the color of the mucous membranes were visually noted. After the 10th treatment session, the patient was discharged home in satisfactory condition, no complaints. In the next 3 months of observation, persistent remission of this disease was noted.

 Example 4. Serviceman T., 24 years old. He was admitted with a diagnosis of a mine-explosive wound of the left lower limb — tear off of the lower leg (1st level of detachment, crushing and stratification of tissues, an extensive scalped wound of the lower leg. The leg segment was amputated in the first 12 hours after the wound, surgical treatment of the wound of the stump with skin grafting was performed, The patient was delivered to 3 CVCH on the 2nd day after the operation. Upon admission, marked congestion of stump tissue and edema, sutures with serous-purulent plaque, presence of putrefactive odor. Bacteriological and gas chromatographic e study wound content showed a high level of purulent polymicrobial hearth contamination by aerobic and facultative anaerobic bacteria, including Clostridium, wherein the amount of the pathogenic microflora considerably exceed the critical level.

 As a matter of urgency, sparing reamputation of the type of extended necroectomy and secondary surgical treatment of the wound were performed. Necrotic sections of the wound of the stump were excised, lobe incisions were made to prevent ascending infection.

 At the end of the surgical treatment of the site of infection, the wound surface was additionally subjected to ultrasound treatment through an intermediate drug solution in the form of a mixture of a highly active antiseptic with an antibiotic to achieve its high-quality sanitation. Then, a layer of ozonized oil was obtained on the wound surface, obtained according to the method described above in the description of the invention, with a peroxide value of P = 1100 - 1200. Subsequent drainage and dressings in the prescribed manner. In the postoperative period, this stage of wound treatment and the area of sutures was performed daily until it was maximally cleaned of necrotic tissues and the appearance of granulations. Tissue hypoxia is effectively eliminated. Infection and reinfection are prevented. Microbiological control on the 8th day of treatment showed a sharp decrease in bacterial contamination of the wound. VFA - not detected.

 Subsequently, a wound in the phase of dehydration, regeneration, formation and maturation of granulation tissue was treated with a jet-aerosol torch of the mixture: 0.9% saline and antibiotic (95 parts), as well as OTRISAN ozonized olive oil (5 parts) . On the 12th day, the wound was completely cleaned of necrotic tissue, granular, sterile, VFA - absent, which allowed secondary sutures to be applied followed by daily treatment according to the above procedure. Sutures were removed after 7 days, which corresponds to 19 days of the postoperative period. In the near future, complete healing of the stump was noted, the stump scars were in good condition, the functions of the upper extremities were preserved, which made it possible 10 to 12 days earlier than usual to proceed to the next stage - the stage of functional limb prosthetics.

 Example 5. Patient N., 11 years old. Diagnosis: right-sided chronic purulent mesothympanitis. Decrease in hearing in the right ear by 25-30 dV. The duration of the disease is 4 years after the flu. Bacteriological examination of discharge from the ear showed the presence of coccal and rod flora, as well as mycobacteria. The use of well-known medical and physiotherapeutic methods did not achieve a therapeutic effect.

 It was proposed and carried out the treatment using OTRISAN ozonated refined olive oil, obtained according to the method described above in the description of the invention, with a peroxide value of P = 1100 - 1200.

 At the first stage of treatment, the external auditory canal and the middle ear cavities were washed with a 2% hydrogen peroxide solution or 1: 5000 furatsilin solution, followed by drying. At the second stage, the auditory meatus and the middle ear cavity were filled with OTRISAN ozonized oil with its retention in the tympanic cavity for 5-10 minutes.

 The treatment was carried out daily and consisted of 15 sessions. Antibiotics and fungicidal drugs were not used.

 5-6 days after the start of treatment, suppuration stopped. Bactericidal and fungicidal effects, as well as a dry ear, were obtained after the 10th session. By the 15th treatment session, epithelization of perforation of the tympanic membrane and audiometrically confirmed improvement in hearing in the right ear by an average of 20 dV were noted. During the checkup of the patient's condition after 3 and 6 months, no recurrence of the disease was found. The achieved improvement in hearing is saved.

 The inventive method for producing ozonized oil "OTRISAN" is a highly effective method that allows you to get a medicinal substance with high biological activity and long-term preservation of its anti-inflammatory, bactericidal, virucidal, fungicidal and detoxification activities. OTRISAN ozonized oil can be used in the treatment of inflammatory diseases and infected wounds, as well as viral infections in infectology, general surgery, orthopedics and traumatology, otorhinolaryngology, gynecology, dentistry, neurosurgery, etc.

Claims (2)

 1. A method of producing ozonized oil by sparging a vegetable oil with an ozone-containing gas component, characterized in that the oil is additionally exposed to ultrasound at a low frequency, and a bubbling ozone-containing gas component is directionally sprayed into the developed cavitation area of the voiced oil volume. 2. The method according to claim 1, characterized in that the frequency of ultrasonic vibrations is 18 - 44 kHz; the amplitude of ultrasonic vibrations - 30 - 40 microns, the exposure of ultrasonic exposure - 1 - 2 s per 1 cm ^{3 the} volume of the sound oil; the flow rate of the ozone-containing gas component is not less than 500 ml / min per 1 dm ^{3 of the} volume of the ultrasound chamber; the ozone concentration in the ozone-containing gas component is not less than 5 mg / l.

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