RU2289413C2 - Method for ozonizing of physiological salt solution - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: method involves providing double-staged processing of physiological salt solution with ozone, first stage including bubbling of physiological salt solution with ozone-oxygen mixture having ozone concentration of 3 mg/l to 10 mg/l at ozonizer outlet end, and second stage including additionally introducing ozone-oxygen mixture into the same bottle under pressure of from 1.5 atm to 2.9 atm, said mixture having ozone concentration of from 6 mg/l to 10 mg/l.

EFFECT: increased time of keeping ozone in physiological salt solution.

3 tbl, 1 ex

Description

The present invention relates to medicine, namely to drug therapy, and can be used in surgery and therapy with intravenous and local ozone therapy.

Ozone therapy is a fairly new non-drug treatment that is becoming more common. This is due to the fact that along with significant successes in the development of pharmacotherapy, non-drug methods of treatment are increasingly being used - physiotherapy, acupuncture, hirudotherapy, manual therapy, etc. Treatment with ozone-oxygen mixtures represents a qualitatively new solution to the urgent problems of the treatment of many diseases, this is due to the properties of ozone to influence the transport and release of oxygen in tissues, its disinfecting properties. This circumstance leads to a wide range of ozone therapy. It is used in therapy, surgery, obstetrics and gynecology, dermatology, dentistry. Ozone therapy is characterized by ease of use, high efficiency, good tolerance, and virtually no side effects. This type of therapy is cost effective. The use of ozone in practical medicine has long been constrained by the prevailing opinion of its toxicity in connection with the use of high concentrations of ozone in industry. However, in clinical practice, ozone concentrations are several orders of magnitude lower than toxic. In the range of these concentrations, ozone acts as a therapeutic agent and exhibits anti-inflammatory, bactericidal, viricidal, fungicidal, analgesic effects. Ozone therapy is used in the form of enteral and parenteral administration of ozone-oxygen mixtures, closed aeration, applications with ozonized materials. An important condition for treatment is the dose of ozone administered, which should not exceed the potential of antioxidant enzymes.

For the prototype of the invention, a well-known method of ozonation of physiological saline is selected by sparging a physiological saline with an ozone-oxygen mixture with an ozone concentration of 3 to 10 mg / l at the outlet of the ozonizer (see. "Ozone Therapy. Internal Medicine" O.V. Maslennikov, K.N. Kontorshchikova, N. Novgorod, 2003, p. 39). The method is as follows: the ozone generator is connected to an oxygen source and to the power grid, the ozone concentration in the ozone-oxygen mixture and the exposure are set on the panel of the device. A long needle is inserted into the cork, reaching the bottom of the bottle, and a short needle not reaching the surface of the solution, a polyvinyl chloride tube is connected to the long needle from the outlet of the apparatus, an ozone destructor is connected to the short needle, the apparatus is turned on and bubbling is carried out for up to 10 minutes.

The finished solution is used intravenously or for the treatment of wounds. The preparation of the solution involves a sufficiently high concentration of ozone in the solution - up to 6 mg / L.

The disadvantage of this method is the rapid decay of ozone. According to various authors (Boyarinov G.A., Boyarinova L.V., Sokolov V.V., Gordetsov A.S., Kalyagina S.G., Kalashnikov S.P., Zhivulin A.S. “Ozone decay in saline solution. "Ozone and methods of efferent therapy in medicine. III All-Russian Scientific and Practical Conference September 16-18, 1998, abstracts. S.9-11) the concentration of ozone in saline solution at room temperature after 5 minutes is reduced by 17 %, after 10 minutes - by 29%, after 15 minutes - by 37%, after 20 minutes - by 43%, after 25 minutes - by 46%, and after 30 minutes - by half. After preparation, the solution should be used as soon as possible.

The task of the invention is to enable longer-term preservation of the concentration of ozone and its biological activity in a bottle of ozonized physiological solution.

The problem is solved in that in the known method of ozonation of physiological saline, including bubbling of physiological saline with an ozone-oxygen mixture with an ozone concentration at the outlet of the apparatus from 3 to 10 mg / l, a two-stage treatment of saline solution is carried out, and after completion of the bubbling of saline with an ozone-oxygen mixture into a bottle with an ozonated saline solution is additionally introduced an ozone-oxygen mixture under pressure from 1.5 to 2.9 atm with an ozone concentration at the outlet of from 6 to 10 mg / L.

The present invention meets the criteria of "novelty" and "inventive step", since in the process of conducting patent information research, no sources of information were found discrediting the novelty of the proposed method, as well as technical solutions with essential features of the proposed method.

Our proposed method consists in a two-stage processing solution. The first stage is the bubbling of the solution with an ozone-oxygen mixture until the required concentration of ozone is obtained. The second stage in the same bottle is injected ozone-oxygen mixture under pressure from 1.5 to 2.9 atm with an ozone concentration of from 6 to 10 mg / L. The introduction of ozone under high pressure maintains the ozone content in the solution, preserves its therapeutic properties for a long time.

Our studies show that when stored with hyperbaric support, ozonized physiological saline retains bactericidal properties for up to 2 hours, while without hyperbaric support it retains them only for 30 minutes.

When spectrophotometric study it was noted that one hour after ozonation, the ozone content in the solution ozonized by the proposed method is two times greater than in the bottle ozonized by the prototype method. This can be seen in tables 1-3.

Table 1
Measurement of ozone concentration in saline Source After 30 min After 60 min The solution is ozonated in the traditional way 0.3 0.2 0.2 1.3 0.7 0.4 one hundred% fifty% 25% A solution prepared with hyperbaric support 1,0 0.4 0.6 1,6 1,0 0.6 75% 60%

table 2
Comparative study of the bactericidal activity of ozonized physiological saline and ozonized solution with hyperbaric support against Staphylococcus aureus Time Control (unzoned physical solution) ODF 5000 mcg / L OFR + HBO ₃ 10,000 mcg / L 0 min 6804 colonies per cup No growth No growth 30 min (exposure with germs) Solid growth 3061 colonies per cup No growth 1 hour (exposure with germs) Solid growth 4531 colonies per cup No growth 2 hours (exposure with germs) Solid growth 5670 colonies per cup 2268 colonies per cup 24 hours (exposure with germs) Solid growth Solid growth Solid growth Table 3
Comparative study of the bactericidal activity of ozonized physiological saline and ozonized solution with hyperbaric support against Pseudomonas aeruginosa Time Control (unzoned physical solution) ODF 5000 mcg / L OFR + HBO ₃ 10,000 mcg / L 0 min 8958 colonies per cup No growth No growth 30 min (exposure with germs) Solid growth 3402 colonies per cup No growth

1 hour (exposure with germs) Solid growth 5273 colonies per cup No growth 2 hours (exposure with germs) Solid growth 5577 colonies per cup 3175 colonies per cup 24 hours (exposure with germs) Solid growth Solid growth Solid growth

Findings:

DFS during storage with hyperbaric support retains its bactericidal properties for a longer period (up to 2 hours).

Example.

The method is as follows. The ozone generator is connected to an oxygen source and to the power supply network, and an ozone concentration of 3 to 10 mg / l in the ozone-oxygen mixture and exposure are set on the instrument panel. A long needle is inserted into the cork, reaching the bottom of the vial, and a short needle, not reaching the surface of the solution. A polyvinyl chloride tube is attached to the long needle from the outlet of the ozone generator, and an ozone destructor is attached to the short needle. The ozone generator is turned on and sparging is carried out for up to 10 minutes. Immediately after the bubbling is complete, airway needles are removed from the vial. The necessary ozone concentration is set on the panel of the ozone generator from 6 to 10 mg / l, the ozone generator is turned on, 100 ml of the ozone-oxygen mixture are collected in the Jean syringe and, by piercing the tube with a thin needle, the gas mixture is introduced into the vial under pressure from 1.5 to 2.9 atm

Claims (1)

A method of ozonizing a physiological solution, including bubbling a physiological solution with an ozone-oxygen mixture with an ozone concentration of 3 to 10 mg / l at the outlet of the ozonizer, characterized in that two-stage treatment of the saline solution is carried out, while after the completion of the bubbling of the saline with an ozone-oxygen mixture into a bottle with an ozonized physiological solution is additionally injected with an ozone-oxygen mixture under increased pressure from 1.5 to 2.9 atm with an ozone concentration of from 6 to 10 mg / L.