RU2111495C1 - Method of malignant tumor diagnosis - Google Patents

Abstract

FIELD: medicine, oncology. SUBSTANCE: method involves study of erythrocyte precipitation rate caused by two agents: antiidnotinic antiembryonal serum and control serum. The first agent: rat serum. The second agent: rat serum to whom lymphocytes from intact syngenic animals are administrated preliminary. Minimal and maximal indices of erythrocyte precipitation rate are calculated. On the basis of obtained values coefficient of malignant growth is measured and malignant tumor is determined at its value from 1.55 to 7.00. EFFECT: improved method of diagnosis. 1 tbl

Description

 The invention relates to medicine, namely to the diagnosis of malignant cell growth in a living organism.

 Currently, the most common are gray and histological methods, however, they are quite complicated in execution, require expensive reagents and specially trained personnel (EP 0305337, EP 0285059, EP 0313005). Modern serological methods are specific, for their implementation requires a large set of different diagnostic kits, this causes a rise in the cost of research, not suitable for mass examination of the population and for a long time.

 Closest to the claimed is a method for the diagnosis of tumors, including the use of the erythrocyte sedimentation reaction under the action of the corresponding anti-idiotypic anti-embryonic serum (SU patent 1836640 CL G 01 N 33/80, 93).

 This method is suitable for studying a wide range of diseases of a tumor nature and does not allow to differentiate precisely the malignant growth of tumor cells from benign, but only from diseases of a non-tumor nature and norm.

 The aim of the present invention is to develop a universal rapid method for detecting malignant growth of tumor cells based on the study of erythrocyte sedimentation rate (ESR) under the action of two agents: anti-idiotypic anti-embryonic rat serum (working agent) and rat serum (control agent), which are pre-injected with intact syngenic lymphocytes animals with the subsequent calculation of the coefficient of malignancy of cell growth.

The method is as follows: take 100 ml of blood from a patient and add 20 μl of each of the above two agents (separately), shake and place in the capillaries for ESR for 1 hour at 37 ^o C.

где
К_эр - коэффициент злокачественного роста
С_макс - максимальный уровень градиента СОЭ
С_мин - минимальный уровень градиента СОЭ
и при значении К_эр = 1,55 - 7,00 лпределяют злокачественный рост клеток.Then measure the ESR gradient in each sample, find the maximum and minimum indicator, and the coefficient of malignant cell growth (K _er ) is determined by the formula:

Where
To _er - the coefficient of malignant growth
C _max - maximum ESR gradient level
C _min - the minimum level of ESR gradient
and when the value of K _er = 1.55 - 7.00 l determine the malignant growth of cells.

 The method is illustrated by the following examples.

 Example 1. B-naya Smirnova T.V. - 46 years old, N history of whiteness 2948/95, diagnosis: breast cancer I B st. ESR with working serum - 20 mm; with a control of 16 mm.

Пример 2. Б-ной Андреичев Ю.В., 43 г., история болезни N 2846/95, диагноз: рак желудка III Б си. СОЭ с рабочей сывороткой - 25 мм; с контрольной - 13 мм.

Example 2. Bnoy Andreichev Yu.V., 43 g., Medical history N 2846/95, diagnosis: gastric cancer III B si. ESR with working serum - 25 mm; with a control - 13 mm.

Пример 3. И. С. Б. - 32 года. СОЭ с рабочей сывороткой - 9 мм; с контрольной - 14 мм.

Example 3. I. S. B. - 32 years. ESR with working serum - 9 mm; with a control - 14 mm.

- практически здоров.

- almost healthy.

 Example 4

 Sick Ivanova R.S. - 36 years old, medical history N 2964/95, diagnosis: cystic fibrous mastopathy, ESR with working serum - 12 mm; with a control - 6 mm.

Исследование проведено на 1600 пациентах в различных клиниках Российской Федерации. В частности исследования проведенные в Московской медицинской академии показали следующие результаты (таблица).

The study was conducted on 1600 patients in various clinics of the Russian Federation. In particular, studies conducted at the Moscow Medical Academy showed the following results (table).

 The table shows that the sensitivity of the method is very high and is achieved in some cases 100%, confirming the high diagnostic value of the method.

 The method for diagnosing malignant tumors is technically simple, universal, has high sensitivity and specificity, which allows us to recommend it for wide practical use in medical institutions.

Claims (1)

A method for diagnosing a malignant tumor by examining the erythrocyte sedimentation rate under the action of two agents - anti-idiotypic anti-embryonic serum and control serum, characterized in that rat serum is used as the first agent, and rat serum, which are previously injected with lymphocytes of intact syngenic animals, is found to be minimal and the maximum ESR gradient, according to the obtained values determine the coefficient of malignancy of cell growth according to the formula

where K _{s p} - malignancy coefficient of growth;
With _{m a to s} - the maximum level of the ESR gradient;
With _{m and n} - the minimum level of ESR gradient,
and when the value of K _{s p} = 1.55 - 7.0 determine a malignant tumor.

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