RU2060275C1 - Способ получения человеческого лизоцима - Google Patents

Способ получения человеческого лизоцима Download PDF

Info

Publication number
RU2060275C1
RU2060275C1 SU894742058A SU4742058A RU2060275C1 RU 2060275 C1 RU2060275 C1 RU 2060275C1 SU 894742058 A SU894742058 A SU 894742058A SU 4742058 A SU4742058 A SU 4742058A RU 2060275 C1 RU2060275 C1 RU 2060275C1
Authority
RU
Russia
Prior art keywords
lysozyme
preparing
human lysozyme
produce
secrete
Prior art date
Application number
SU894742058A
Other languages
English (en)
Inventor
Д.В.Анотье Жак
де Батселье Анни
Розенберг Стивен
Original Assignee
Лябофина С.А.
Чирон Корпорейшн
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Лябофина С.А., Чирон Корпорейшн filed Critical Лябофина С.А.
Application granted granted Critical
Publication of RU2060275C1 publication Critical patent/RU2060275C1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Использование: изобретение относится к способу получения активного человеческого лизоцима посредством культивирования трансформированных дрожжей. Сущность изобретения: способ получения человеческого лизоцима предусматривает конструирование рекомбинантной плазмидной ДНК, содержащей гибридный промотор АДН 2 - G AP, сигнальную последовательность куриного лизоцима или α -фактора дрожжей, синтетический ген и АДН 2 или GAP терминатор, трансформацию полученной ДНК штамма Saccharomuces cerevisial GRF 180, индукцию трансформантов путем дерепрессии промотора, выделение и очистку целового продукта из культуральной жидкости трансформантов. 10 ил., 7 табл.
SU894742058A 1988-09-26 1989-09-25 Способ получения человеческого лизоцима RU2060275C1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24941388A 1988-09-26 1988-09-26
US249413 1988-09-26

Publications (1)

Publication Number Publication Date
RU2060275C1 true RU2060275C1 (ru) 1996-05-20

Family

ID=22943380

Family Applications (1)

Application Number Title Priority Date Filing Date
SU894742058A RU2060275C1 (ru) 1988-09-26 1989-09-25 Способ получения человеческого лизоцима

Country Status (10)

Country Link
US (1) US5585257A (ru)
EP (1) EP0362183B1 (ru)
JP (1) JP2905230B2 (ru)
AT (1) ATE90726T1 (ru)
DE (1) DE68907166T2 (ru)
DK (1) DK175315B1 (ru)
ES (1) ES2057183T3 (ru)
IE (1) IE63964B1 (ru)
NO (1) NO177065C (ru)
RU (1) RU2060275C1 (ru)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03155791A (ja) * 1989-11-14 1991-07-03 Green Cross Corp:The 雑種プロモーター
US20080050503A1 (en) * 2000-05-02 2008-02-28 Ning Huang Expression of human milk proteins in transgenic plants
US7417178B2 (en) * 2000-05-02 2008-08-26 Ventria Bioscience Expression of human milk proteins in transgenic plants
US7138150B2 (en) * 2000-05-02 2006-11-21 Ventria Bioscience Method of making an anti-infective composition for treating oral infections
NZ529324A (en) * 2001-04-24 2005-08-26 Innogenetics N Core-glycosylated HCV envelope proteins made in yeast cells
JP5414972B2 (ja) * 2003-12-23 2014-02-12 バイオキット， エセ．アー． 病原体感染の検出のための組成物および方法
CN100451119C (zh) * 2005-12-08 2009-01-14 辽宁省农业科学院大连生物技术研究所 柞蚕溶菌酶的基因序列及其表达产物的制备方法
CN105567714A (zh) * 2014-11-07 2016-05-11 西南民族大学 一种重组牦牛溶菌酶及其制备方法和用途
US20240034961A1 (en) * 2020-12-16 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4595658A (en) * 1982-09-13 1986-06-17 The Rockefeller University Method for facilitating externalization of proteins synthesized in bacteria
US4624922A (en) * 1983-12-09 1986-11-25 Rikagaku Kenkyusho Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism
US4880734A (en) * 1984-05-11 1989-11-14 Chiron Corporation Eukaryotic regulatable transcription
EP0480480A3 (en) * 1984-05-11 1992-06-10 Chiron Corporation Enhanced yeast transcription employing hybrid promoter region constructs
US4767708A (en) * 1984-08-07 1988-08-30 Carnegie Mellon University Enzyme amplification and purification
BE901223A (fr) * 1984-12-06 1985-03-29 Labofina Sa Levures transformees produisant du lysozyme plasmides utilises pour cette transformation et leurs utilisations.
JPS626679A (ja) * 1985-06-26 1987-01-13 Agency Of Ind Science & Technol ヒトリゾチ−ムの微生物学的製造法
GB8521496D0 (en) * 1985-08-29 1985-10-02 Ciba Geigy Ag Repressible yeast promoters
BE903626A (fr) * 1985-11-13 1986-03-03 Labofina Sa Procede pour la recuperation de polypeptides localises dans l'espace periplasmique de la levure.
US5124256A (en) * 1985-11-13 1992-06-23 Labofina, S.A. Process for recovering polypeptides localized in the periplasmic space of yeast without breaking the cell wall by using an non-ionic detergent and a neutral salt
IL83435A (en) * 1986-09-23 1993-01-14 Miles Lab Dna construct for expression of genes in yeast, an expression vector containing it and yeast transformed with said expression vector

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Jamamoto et. at., Biochem. and Biophis. Res Comm., 149, 431 - 436, 1987. *

Also Published As

Publication number Publication date
NO893553D0 (no) 1989-09-05
EP0362183B1 (en) 1993-06-16
IE893053L (en) 1990-03-26
DK175315B1 (da) 2004-08-16
JP2905230B2 (ja) 1999-06-14
DE68907166T2 (de) 1993-10-21
DK460389A (da) 1990-03-27
ES2057183T3 (es) 1994-10-16
IE63964B1 (en) 1995-06-28
NO893553L (no) 1990-03-27
EP0362183A2 (en) 1990-04-04
DE68907166D1 (de) 1993-07-22
NO177065B (no) 1995-04-03
US5585257A (en) 1996-12-17
EP0362183A3 (en) 1990-08-08
ATE90726T1 (de) 1993-07-15
JPH02156885A (ja) 1990-06-15
NO177065C (no) 1995-07-12
DK460389D0 (da) 1989-09-19

Similar Documents

Publication Publication Date Title
AU7111091A (en) Process for production of b avermectins and cultures therefor
AU4277689A (en) Process for producing cultured epidermal sheet, product and method of use
AP8800077A0 (en) Process for production of avermecting and cultures thereof
EP0248637A3 (en) Induction of galactose regulated gene expression in yeast
RU2060275C1 (ru) Способ получения человеческого лизоцима
AU604900B2 (en) Process for producing novel yeast and mold inhibiting products
HUT46362A (en) Process for the enhancement of the production of a/16686 antibiotic complex and for the selective increase of the proportion of certain components of the complex
EP0121884A3 (en) Hybrid dna synthesis of insulin
DK165757C (da) Biologisk ren kultur af mikroorganismen streptomyces roseosporus nrrl 15998 samt en fremgangsmaade til fremstilling af det antibiotisk virksomme a-21978c kompleks eller en eller flere af dettes faktorer co' c1' c2' c3' c4' og c5' under anvendelse af mikroorganismen
EP0649900A3 (en) Protein production process.
IE872420L (en) Preparing antibiotic a40926 complex
CA2115042A1 (en) A Fermentation Process for Producing Natamycin
CA2002480A1 (en) Process for the production of human lysozyme
AU1551988A (en) Growth promoter for bifidobacterium species, process for preparing the same and method of using the same
EP0279665A3 (en) A method of regulating expression of a foreign gene by controlling culture temperature and a process of producing a foreign gene product thereby
EP0280407A3 (en) Process for the production of recombinant polypeptides with porcine growth hormone activity
EP0287123A3 (en) L-valine producing microorganism and a process for producing l-valine by fermentation
EP0101273A3 (en) Production of biological products using resting cells
ATE22702T1 (de) Herstellung von glucosedehydrogenase.
DE3677421D1 (de) Mikrobiologisches verfahren zur herstellung von menschlichem lysozym.
EP0279664A3 (en) A method of regulating expression of a foreign gene by controlling the sugar concentration in a medium and a process of producing a foreign gene product thereby
IL81751A (en) Continuous fermentation process for producing bacterial cells comprising use of high mineral salt feed for high yields and high productivity
PL251347A2 (en) Method of controlling feed-in of culture medium during a continuous yeast cultivation process
AP8800088A0 (en) Process for production of bavermectins and cultures therefor
AU603416C (en) Process for production of B avermectins and cultures therefor

Legal Events

Date Code Title Description
PD4A Correction of name of patent owner