RU2018111219A - Культура клеток - Google Patents
Культура клеток Download PDFInfo
- Publication number
- RU2018111219A RU2018111219A RU2018111219A RU2018111219A RU2018111219A RU 2018111219 A RU2018111219 A RU 2018111219A RU 2018111219 A RU2018111219 A RU 2018111219A RU 2018111219 A RU2018111219 A RU 2018111219A RU 2018111219 A RU2018111219 A RU 2018111219A
- Authority
- RU
- Russia
- Prior art keywords
- dimensional
- medium
- ali
- spheroid
- liver cells
- Prior art date
Links
- 238000004113 cell culture Methods 0.000 title 1
- 210000005229 liver cell Anatomy 0.000 claims 33
- 239000002609 medium Substances 0.000 claims 31
- 210000000056 organ Anatomy 0.000 claims 23
- 239000003795 chemical substances by application Substances 0.000 claims 15
- 230000002685 pulmonary effect Effects 0.000 claims 11
- 210000002919 epithelial cell Anatomy 0.000 claims 10
- 239000001963 growth medium Substances 0.000 claims 10
- 238000003501 co-culture Methods 0.000 claims 9
- 210000004027 cell Anatomy 0.000 claims 8
- 238000000034 method Methods 0.000 claims 7
- 238000012258 culturing Methods 0.000 claims 5
- 239000006143 cell culture medium Substances 0.000 claims 4
- 238000006243 chemical reaction Methods 0.000 claims 4
- 239000000443 aerosol Substances 0.000 claims 3
- 238000005259 measurement Methods 0.000 claims 3
- 102000009027 Albumins Human genes 0.000 claims 2
- 108010088751 Albumins Proteins 0.000 claims 2
- 102100031476 Cytochrome P450 1A1 Human genes 0.000 claims 2
- 101710104049 Cytochrome P450 1A1 Proteins 0.000 claims 2
- 102000012466 Cytochrome P450 1B1 Human genes 0.000 claims 2
- 108050002014 Cytochrome P450 1B1 Proteins 0.000 claims 2
- 230000036755 cellular response Effects 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 238000000338 in vitro Methods 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 230000021368 organ growth Effects 0.000 claims 2
- 230000035515 penetration Effects 0.000 claims 2
- 230000028327 secretion Effects 0.000 claims 2
- 239000000779 smoke Substances 0.000 claims 2
- 235000019504 cigarettes Nutrition 0.000 claims 1
- 210000000981 epithelium Anatomy 0.000 claims 1
- 210000004185 liver Anatomy 0.000 claims 1
- 210000005265 lung cell Anatomy 0.000 claims 1
- 239000002547 new drug Substances 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 claims 1
- 230000002110 toxicologic effect Effects 0.000 claims 1
- 231100000027 toxicology Toxicity 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0688—Cells from the lungs or the respiratory tract
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5067—Liver cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/27—Lung cells, respiratory tract cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2513/00—3D culture
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Sustainable Development (AREA)
- Gastroenterology & Hepatology (AREA)
- Pulmonology (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16185725.5A EP3287521B1 (en) | 2016-08-25 | 2016-08-25 | Cell culture |
| EP16185725.5 | 2016-08-25 | ||
| PCT/EP2017/070881 WO2018036910A1 (en) | 2016-08-25 | 2017-08-17 | Cell culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2018111219A3 RU2018111219A3 (https=) | 2019-09-30 |
| RU2018111219A true RU2018111219A (ru) | 2019-09-30 |
Family
ID=56842661
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2018111219A RU2018111219A (ru) | 2016-08-25 | 2017-08-17 | Культура клеток |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11041145B2 (https=) |
| EP (2) | EP3287521B1 (https=) |
| JP (2) | JP6810742B2 (https=) |
| KR (1) | KR20180049000A (https=) |
| CN (1) | CN108138135A (https=) |
| PL (1) | PL3287521T3 (https=) |
| RU (1) | RU2018111219A (https=) |
| WO (1) | WO2018036910A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7530886B2 (ja) * | 2018-05-16 | 2024-08-08 | インセルム（インスティチュート ナショナル デ ラ サンテ エ デ ラ リシェルシェ メディカル） | 増殖性肝細胞を培養する方法 |
| WO2020172337A1 (en) * | 2019-02-19 | 2020-08-27 | Iontox, Llc | Methods to predict repeated dose toxicity using an integrated organ platform |
| CN113699202A (zh) * | 2020-05-21 | 2021-11-26 | 华子昂 | 一种用混合细胞与人工细胞培养巢制备胶原蛋白的方法 |
| JP2024527371A (ja) * | 2021-07-08 | 2024-07-24 | オウロテック インコーポレイテッド | 処置の有効性予測システム及び方法 |
| CN120005810A (zh) * | 2025-04-18 | 2025-05-16 | 立沃生物科技（深圳）有限公司 | 肝祖细胞、应用及3d动态培养扩增方法 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19721113A1 (de) | 1996-05-20 | 1997-11-27 | Sumitomo Chemical Co | Diagnosekit |
| GB9913979D0 (en) | 1999-06-17 | 1999-08-18 | Univ Wales Medicine | Spheroid preparation |
| US7351584B2 (en) | 2002-02-26 | 2008-04-01 | In Vitro Technologies, Inc. | Hepatocyte bioreactor system for long term culture of functional hepatocyte spheroids |
| EP1496066A4 (en) | 2002-03-25 | 2006-09-13 | Japan Science & Tech Agency | ANTIBODY RECOGNIZING PROLIFERATIVE HUMAN LIVER CELLS, PROLIFERATIVE HUMAN LIVER CELLS, AND FUNCTIONAL HUMAN LIVER CELLS |
| US7753192B2 (en) | 2005-12-23 | 2010-07-13 | The Coca-Cola Company | Apparatus and method for orienting spheroidal containers and packaging beverages in spheroidal containers |
| JP5217220B2 (ja) | 2007-04-12 | 2013-06-19 | 株式会社日立製作所 | 細胞分離装置 |
| JP2010534065A (ja) * | 2007-07-20 | 2010-11-04 | セルアーティス アーベー | ヒト胚盤胞幹細胞に、胚体内胚葉（ＤＥ‐ｈｅｐ）を介して由来する新規な肝細胞の集団 |
| US20110262956A1 (en) | 2008-10-07 | 2011-10-27 | Guillermo Munoz Elias | Co-culture compositions and methods |
| US8530237B2 (en) | 2009-01-08 | 2013-09-10 | Hitachi, Ltd. | Method for culturing animal hepatocyte |
| GB201104511D0 (en) * | 2011-03-17 | 2011-05-04 | Univ Cardiff | Cell based screening assay |
| JP5812816B2 (ja) | 2011-11-15 | 2015-11-17 | コバレントマテリアル株式会社 | 細胞培養担体及びその製造方法 |
| DE102011121317A1 (de) | 2011-12-16 | 2013-06-20 | Universitätsklinikum Hamburg-Eppendorf | Gerüstfreie Leberzellaggregate zur Verwendung bei der Transplantation |
| CN102559581B (zh) * | 2012-01-20 | 2014-09-10 | 四川大学华西医院 | 一种无血清肝细胞培养基 |
| US10677783B2 (en) | 2012-09-27 | 2020-06-09 | Corning Incorporated | Method for evaluating effect of cytokine on metabolic activity of cytochrome P450, and drug screening method |
| US20140106356A1 (en) | 2012-10-12 | 2014-04-17 | National Taiwan University | Kit for drug metabolism determination and toxicity prediction |
| US9442105B2 (en) * | 2013-03-15 | 2016-09-13 | Organovo, Inc. | Engineered liver tissues, arrays thereof, and methods of making the same |
| WO2015163043A1 (ja) | 2014-04-22 | 2015-10-29 | 株式会社日本触媒 | フッ素含有ポリマーを表面に含む細胞培養用基材 |
| JP6681664B2 (ja) * | 2014-09-05 | 2020-04-15 | 株式会社日本触媒 | 含フッ素ポリイミドを含む酸素ガス透過性の細胞培養用基材、該基材を備えた細胞培養用容器、及び、該基材を用いた細胞培養方法 |
-
2016
- 2016-08-25 EP EP16185725.5A patent/EP3287521B1/en active Active
- 2016-08-25 EP EP21180293.9A patent/EP3940059A1/en not_active Withdrawn
- 2016-08-25 PL PL16185725T patent/PL3287521T3/pl unknown
-
2017
- 2017-08-17 JP JP2018519751A patent/JP6810742B2/ja active Active
- 2017-08-17 CN CN201780003424.5A patent/CN108138135A/zh active Pending
- 2017-08-17 RU RU2018111219A patent/RU2018111219A/ru unknown
- 2017-08-17 US US15/757,415 patent/US11041145B2/en active Active
- 2017-08-17 KR KR1020187009401A patent/KR20180049000A/ko not_active Ceased
- 2017-08-17 WO PCT/EP2017/070881 patent/WO2018036910A1/en not_active Ceased
-
2020
- 2020-12-11 JP JP2020205752A patent/JP7097942B2/ja not_active Expired - Fee Related
-
2021
- 2021-05-12 US US17/318,056 patent/US11981926B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3287521A1 (en) | 2018-02-28 |
| JP2021048865A (ja) | 2021-04-01 |
| PL3287521T3 (pl) | 2021-12-20 |
| JP7097942B2 (ja) | 2022-07-08 |
| US11981926B2 (en) | 2024-05-14 |
| US20190119647A1 (en) | 2019-04-25 |
| EP3287521B1 (en) | 2021-06-23 |
| US20210269774A1 (en) | 2021-09-02 |
| JP6810742B2 (ja) | 2021-01-06 |
| KR20180049000A (ko) | 2018-05-10 |
| EP3940059A1 (en) | 2022-01-19 |
| JP2019506134A (ja) | 2019-03-07 |
| US11041145B2 (en) | 2021-06-22 |
| RU2018111219A3 (https=) | 2019-09-30 |
| CN108138135A (zh) | 2018-06-08 |
| WO2018036910A1 (en) | 2018-03-01 |
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