CN108138135A - 细胞培养 - Google Patents

细胞培养 Download PDF

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Publication number
CN108138135A
CN108138135A CN201780003424.5A CN201780003424A CN108138135A CN 108138135 A CN108138135 A CN 108138135A CN 201780003424 A CN201780003424 A CN 201780003424A CN 108138135 A CN108138135 A CN 108138135A
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CN
China
Prior art keywords
medium
complete
dimensional
ali
william
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780003424.5A
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English (en)
Chinese (zh)
Inventor
D·博瓦德
K·吕蒂希
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Philip Morris Products SA
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Philip Morris Products SA
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Publication of CN108138135A publication Critical patent/CN108138135A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/14—Coculture with; Conditioned medium produced by hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/27—Lung cells, respiratory tract cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Sustainable Development (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pulmonology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201780003424.5A 2016-08-25 2017-08-17 细胞培养 Pending CN108138135A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16185725.5A EP3287521B1 (en) 2016-08-25 2016-08-25 Cell culture
EP16185725.5 2016-08-25
PCT/EP2017/070881 WO2018036910A1 (en) 2016-08-25 2017-08-17 Cell culture

Publications (1)

Publication Number Publication Date
CN108138135A true CN108138135A (zh) 2018-06-08

Family

ID=56842661

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780003424.5A Pending CN108138135A (zh) 2016-08-25 2017-08-17 细胞培养

Country Status (8)

Country Link
US (2) US11041145B2 (https=)
EP (2) EP3287521B1 (https=)
JP (2) JP6810742B2 (https=)
KR (1) KR20180049000A (https=)
CN (1) CN108138135A (https=)
PL (1) PL3287521T3 (https=)
RU (1) RU2018111219A (https=)
WO (1) WO2018036910A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113699202A (zh) * 2020-05-21 2021-11-26 华子昂 一种用混合细胞与人工细胞培养巢制备胶原蛋白的方法
CN120005810A (zh) * 2025-04-18 2025-05-16 立沃生物科技（深圳）有限公司 肝祖细胞、应用及3d动态培养扩增方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7530886B2 (ja) * 2018-05-16 2024-08-08 インセルム（インスティチュート ナショナル デ ラ サンテ エ デ ラ リシェルシェ メディカル） 増殖性肝細胞を培養する方法
WO2020172337A1 (en) * 2019-02-19 2020-08-27 Iontox, Llc Methods to predict repeated dose toxicity using an integrated organ platform
JP2024527371A (ja) * 2021-07-08 2024-07-24 オウロテック インコーポレイテッド 処置の有効性予測システム及び方法

Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2012123712A1 (en) * 2011-03-17 2012-09-20 University College Cardiff Consultants Limited Cell-based screening assay
CN105209605A (zh) * 2013-03-15 2015-12-30 奥加诺沃公司 工程化肝组织、其阵列及其制备方法

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DE19721113A1 (de) 1996-05-20 1997-11-27 Sumitomo Chemical Co Diagnosekit
GB9913979D0 (en) 1999-06-17 1999-08-18 Univ Wales Medicine Spheroid preparation
US7351584B2 (en) 2002-02-26 2008-04-01 In Vitro Technologies, Inc. Hepatocyte bioreactor system for long term culture of functional hepatocyte spheroids
EP1496066A4 (en) 2002-03-25 2006-09-13 Japan Science & Tech Agency ANTIBODY RECOGNIZING PROLIFERATIVE HUMAN LIVER CELLS, PROLIFERATIVE HUMAN LIVER CELLS, AND FUNCTIONAL HUMAN LIVER CELLS
US7753192B2 (en) 2005-12-23 2010-07-13 The Coca-Cola Company Apparatus and method for orienting spheroidal containers and packaging beverages in spheroidal containers
JP5217220B2 (ja) 2007-04-12 2013-06-19 株式会社日立製作所 細胞分離装置
JP2010534065A (ja) * 2007-07-20 2010-11-04 セルアーティス アーベー ヒト胚盤胞幹細胞に、胚体内胚葉（ＤＥ‐ｈｅｐ）を介して由来する新規な肝細胞の集団
US20110262956A1 (en) 2008-10-07 2011-10-27 Guillermo Munoz Elias Co-culture compositions and methods
US8530237B2 (en) 2009-01-08 2013-09-10 Hitachi, Ltd. Method for culturing animal hepatocyte
JP5812816B2 (ja) 2011-11-15 2015-11-17 コバレントマテリアル株式会社 細胞培養担体及びその製造方法
DE102011121317A1 (de) 2011-12-16 2013-06-20 Universitätsklinikum Hamburg-Eppendorf Gerüstfreie Leberzellaggregate zur Verwendung bei der Transplantation
CN102559581B (zh) * 2012-01-20 2014-09-10 四川大学华西医院 一种无血清肝细胞培养基
US10677783B2 (en) 2012-09-27 2020-06-09 Corning Incorporated Method for evaluating effect of cytokine on metabolic activity of cytochrome P450, and drug screening method
US20140106356A1 (en) 2012-10-12 2014-04-17 National Taiwan University Kit for drug metabolism determination and toxicity prediction
WO2015163043A1 (ja) 2014-04-22 2015-10-29 株式会社日本触媒 フッ素含有ポリマーを表面に含む細胞培養用基材
JP6681664B2 (ja) * 2014-09-05 2020-04-15 株式会社日本触媒 含フッ素ポリイミドを含む酸素ガス透過性の細胞培養用基材、該基材を備えた細胞培養用容器、及び、該基材を用いた細胞培養方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012123712A1 (en) * 2011-03-17 2012-09-20 University College Cardiff Consultants Limited Cell-based screening assay
CN105209605A (zh) * 2013-03-15 2015-12-30 奥加诺沃公司 工程化肝组织、其阵列及其制备方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113699202A (zh) * 2020-05-21 2021-11-26 华子昂 一种用混合细胞与人工细胞培养巢制备胶原蛋白的方法
CN120005810A (zh) * 2025-04-18 2025-05-16 立沃生物科技（深圳）有限公司 肝祖细胞、应用及3d动态培养扩增方法

Also Published As

Publication number Publication date
EP3287521A1 (en) 2018-02-28
JP2021048865A (ja) 2021-04-01
PL3287521T3 (pl) 2021-12-20
JP7097942B2 (ja) 2022-07-08
US11981926B2 (en) 2024-05-14
US20190119647A1 (en) 2019-04-25
EP3287521B1 (en) 2021-06-23
US20210269774A1 (en) 2021-09-02
JP6810742B2 (ja) 2021-01-06
KR20180049000A (ko) 2018-05-10
EP3940059A1 (en) 2022-01-19
JP2019506134A (ja) 2019-03-07
US11041145B2 (en) 2021-06-22
RU2018111219A3 (https=) 2019-09-30
RU2018111219A (ru) 2019-09-30
WO2018036910A1 (en) 2018-03-01

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Application publication date: 20180608