RU2016132972A - Монолиты с присоединенными распознающими соединениями, их система и их применение - Google Patents

Монолиты с присоединенными распознающими соединениями, их система и их применение Download PDF

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RU2016132972A
RU2016132972A RU2016132972A RU2016132972A RU2016132972A RU 2016132972 A RU2016132972 A RU 2016132972A RU 2016132972 A RU2016132972 A RU 2016132972A RU 2016132972 A RU2016132972 A RU 2016132972A RU 2016132972 A RU2016132972 A RU 2016132972A
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nucleic acid
column
monolith
acid tags
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Пер ХАРБЕРИ
Мадан ПАЙДХУНГАТ
Робин ПРИНС
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Дайс Молекьюлс СВ, ЭлЭлСи
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Claims (25)

1. Колонка, содержащая монолит, содержащий присоединенный полимер, который селективно гибридизуется с тэгом нуклеиновой кислоты, функционально связанным с химически реакционноспособным центром или лигандом.
2. Колонка по п. 1, в которой полимер представляет собой захваченный олигонуклеотид.
3. Колонка по п. 2, в которой захваченный олигонуклеотид содержит от примерно 8 субъединиц нуклеиновых кислот до примерно 50 субъединиц нуклеиновых кислот.
4. Колонка по п. 2, в которой константа скорости связывания тэга нуклеиновой кислоты составляет от примерно 1×102 моль-1с-1 до примерно 1×106 моль-1с-1.
5. Колонка по п. 1, в которой лиганд является пептидом, пептоидом, пептидной нуклеиновой кислотой, депсипептидом или органическим соединением с молекулярной массой менее 2000 дальтон.
6. Колонка по п. 2, в которой захваченный олигонуклеотид ковалентно присоединен к продукту реакции монолита на основе сополимера гицидилметакрилата (ГМА) и диметакрилата этиленгликоля (ДМЭГ) с азидом.
7. Колонка по п. 6, в которой захваченный олигонуклеотид ковалентно присоединяется к продукту реакции монолита на основе сополимера гицидилметакрилата (ГМА) и диметакрилата этиленгликоля (ДМЭГ) с азидом посредством клик-циклоприсоединения.
8. Колонка по п. 1, в которой проницаемость монолита составляет от примерно 1×10-3 мкм2 (1×10-3 дарси) до примерно 1×103 мкм2 (1×103 дарси).
9. Колонка по п. 1, которая связывает от примерно 0,5 фмоль/мкл до примерно 0,4 нмоль/мкл тэга нуклеиновой кислоты.
10. Колонка по п. 1, в которой монолит присоединен к поверхности колонки.
11. Система, содержащая n колонок по п. 1, в которой тэг нуклеиновой кислоты является компонентом смеси тэгов нуклеиновых кислот, и n больше 1.
12. Система, содержащая блок, содержащий n отдельных пространственных положений, в которой каждое пространственное положение содержит монолит, содержащий присоединенный полимер, который селективно гибридизуется с тэгом нуклеиновой кислоты, функционально связанным с химически реакционноспособным центром или лигандом, при этом тег нуклеиновой кислоты является компонентом смеси из n тэгов нуклеиновых кислот, и n представляет собой целое число больше 1.
13. Система по п. 12, в которой полимер представляет собой захваченный олигонуклеотид.
14. Система по п. 12, в которой монолит присоединен к поверхности(ям) пространственных положений, и пространственные положения представляют собой углубления.
15. Система по п. 14, в которой монолит ковалентно присоединен к поверхности пространственных положений посредством образования амидной, сложноэфирной, мочевинной, уретановой, углерод-кремниевой, углерод-азотной, углерод-углеродной, простой эфирной, простой тиоэфирной или дисульфидной связи.
16. Система по п. 12, в которой блок состоит из титана, алюминия, нержавеющей стали, легированных металлов, стекла, кварца, поликарбоната, плавленного кварца, поли(метилметакрилата), пластмассы, полиэфирэфиркетона, полиэфирэфиркетона с добавками, полистирола с добавками, сополимера циклического олефина, ультема на основе полиэфиримида, полипропилена с добавками или их комбинаций.
17. Способ пространственной локализации смеси из n тэгов нуклеиновых кислот, включающий приведение в контакт смеси из n тэгов нуклеиновых кислот с системой по п. 12, тем самым осуществляя пространственную локализацию n подмножеств тэгов нуклеиновых кислот.
18. Способ получения программируемой библиотеки химических соединений ряда нуклеиновых кислот, включающий:
(a) приведение в контакт смеси из n тэгов нуклеиновых кислот с системой по п. 12, тем самым осуществляя пространственную локализацию n подмножеств тэгов нуклеиновых кислот;
(b) элюирование тэгов нуклеиновых кислот в пространственно заданные положения;
(c) осуществление реакции между n пространственно локализованными подмножествами тэгов нуклеиновых кислот и х различающимися химическими субъединицами, при этом х является целым числом больше 1;
(d) объединение в пул n подмножеств из n тэгов нуклеиновых кислот с х различающимися присоединенными химическими субъединицами; и
(e) повторение стадий (a)-(d) к раз, где k - целое число больше 1.
19. Способ по п. 18, в котором х=n или х<n.
20. Способ по п. 18, в котором тэги нуклеиновых кислот, элюированные на стадии (b), иммобилизуются на анионообменной смоле и вымываются с анионообменной смолы после стадии (с).
RU2016132972A 2014-01-28 2015-01-28 Монолиты с присоединенными распознающими соединениями, их система и их применение RU2016132972A (ru)

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PCT/US2015/013382 WO2015116739A1 (en) 2014-01-28 2015-01-28 Monoliths with attached recognition compounds, arrays thereof and uses thereof

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JP2019523646A (ja) * 2016-06-05 2019-08-29 ダイス モレキュルズ エスヴィー， エルエルシーＤｉｃｅ Ｍｏｌｅｃｕｌｅｓ Ｓｖ， Ｌｌｃ 経路決定の方法、組成物、およびその使用
WO2020037194A1 (en) * 2018-08-17 2020-02-20 Sierra Biosystems, Inc. Row-independent oligonucleotide synthesis
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