RU2016119422A - Определение активности фермента в реакциях расщепления - Google Patents

Определение активности фермента в реакциях расщепления Download PDF

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Publication number
RU2016119422A
RU2016119422A RU2016119422A RU2016119422A RU2016119422A RU 2016119422 A RU2016119422 A RU 2016119422A RU 2016119422 A RU2016119422 A RU 2016119422A RU 2016119422 A RU2016119422 A RU 2016119422A RU 2016119422 A RU2016119422 A RU 2016119422A
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Russia
Prior art keywords
cleavage
paragraphs
site
indicator molecule
molecule
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RU2016119422A
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English (en)
Russian (ru)
Inventor
Пол Дэвис
Гита ПАРЕХ
Джеймс ШУТЕН
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Молоджик Лимитед
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Publication of RU2016119422A publication Critical patent/RU2016119422A/ru

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54386Analytical elements
    • G01N33/54387Immunochromatographic test strips
    • G01N33/54388Immunochromatographic test strips based on lateral flow
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/948Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486Metalloendopeptidases (3.4.24)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00Detection or diagnosis of diseases
    • G01N2800/12Pulmonary diseases
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00Detection or diagnosis of diseases
    • G01N2800/12Pulmonary diseases
    • G01N2800/122Chronic or obstructive airway disorders, e.g. asthma COPD
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00Detection or diagnosis of diseases
    • G01N2800/38Pediatrics
    • G01N2800/382Cystic fibrosis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
RU2016119422A 2013-10-23 2014-10-23 Определение активности фермента в реакциях расщепления RU2016119422A (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1318728.1 2013-10-23
GB201318728A GB201318728D0 (en) 2013-10-23 2013-10-23 Detection of cleavage activity of an enzyme
PCT/GB2014/053171 WO2015059487A2 (en) 2013-10-23 2014-10-23 Detection of cleavage activity of an enzyme

Publications (1)

Publication Number Publication Date
RU2016119422A true RU2016119422A (ru) 2017-11-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU2016119422A RU2016119422A (ru) 2013-10-23 2014-10-23 Определение активности фермента в реакциях расщепления

Country Status (11)

Country Link
US (2) US10866236B2 (https=)
EP (1) EP3060915B1 (https=)
JP (1) JP6496322B2 (https=)
CN (1) CN105940304B (https=)
AU (1) AU2014338709B2 (https=)
BR (1) BR112016008956A2 (https=)
CA (1) CA2928308C (https=)
GB (1) GB201318728D0 (https=)
MX (1) MX371379B (https=)
RU (1) RU2016119422A (https=)
WO (1) WO2015059487A2 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201605110D0 (en) 2016-03-24 2016-05-11 Mologic Ltd Detecting sepsis
AU2017248354A1 (en) * 2016-04-08 2018-10-04 Gilead Sciences, Inc. Compositions and methods for treating cancer, inflammatory diseases and autoimmune diseases
CN110268054A (zh) * 2016-11-16 2019-09-20 通用生物传感器有限公司 切割事件转导方法和产品
WO2019043187A1 (en) * 2017-08-31 2019-03-07 Somaprobes Sl SIDE FLOW ASSAY FOR DETECTING THE PRESENCE OF A MAMMALIAN CELL OR SPECIFIC BACTERIUM IN A BIOLOGICAL SAMPLE
EP3692146A4 (en) * 2017-10-04 2021-06-30 The Broad Institute, Inc. DIAGNOSTICS BASED ON CRISPR EFFECTOR SYSTEM
US11732009B2 (en) * 2018-06-08 2023-08-22 Glympse Bio, Inc. Activity sensor with tunable analyte
GB201812331D0 (en) * 2018-07-27 2018-09-12 Mologic Ltd Bacterial vaginosis diagnostic
BR112021012036A2 (pt) * 2018-12-20 2021-09-21 Takeda Pharmaceutical Company Limited Novas sondas baseadas em atividade para elastase neutrofílica e seu uso
EP4211468A4 (en) 2020-09-11 2024-10-09 Glympse Bio, Inc. DETECTION OF EX VIVO PROTEASE ACTIVITY FOR DETECTION/DIAGNOSTIC, STAGING, MONITORING AND TREATMENT OF DISEASES

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4795702A (en) 1986-02-05 1989-01-03 Immunogon Associates Diagnostic method for gonorrhea by assay of IgA1 fragments
WO2003102544A2 (en) 2002-05-31 2003-12-11 Cognosci, Inc. Assays for measuring matrix metalloproteinase activities
EP1452868A2 (en) 2003-02-27 2004-09-01 Pepscan Systems B.V. Method for selecting a candidate drug compound
WO2004099784A2 (en) * 2003-05-06 2004-11-18 Anamar Medical Ab Method for determining a tissue degradation process by detection of fibromodulin neoepitopes
GB0311961D0 (en) 2003-05-23 2003-06-25 Health Prot Agency Mass-based toxin assay and substrates therefor
WO2005024050A1 (en) 2003-09-10 2005-03-17 Antibodyshop A/S Analytical methods for determination of proteolytic cleavage at specific sites
EP1792181B1 (en) * 2004-06-21 2010-11-17 Progenra Inc. Diagnostic and screening methods and kits associated with proteolytic activity
CN101180405A (zh) 2005-05-20 2008-05-14 株式会社三菱化学药得论 酶分析方法
GB2435511A (en) * 2006-02-23 2007-08-29 Mologic Ltd Protease detection
GB2437311A (en) * 2006-04-07 2007-10-24 Mologic Ltd A protease detection product
US7897360B2 (en) * 2006-12-15 2011-03-01 Kimberly-Clark Worldwide, Inc. Enzyme detection techniques
EP2712423A4 (en) 2011-02-25 2015-06-10 Wellstat Diagnostics Llc Assays for detecting enzymatic activity
WO2013116791A1 (en) 2012-02-02 2013-08-08 Mosaic Biosciences, Inc. Biomaterials for delivery of blood extracts and methods of using same
GB201206976D0 (en) 2012-04-20 2012-06-06 Mologic Ltd An enzyme detection device

Also Published As

Publication number Publication date
CA2928308C (en) 2023-07-11
GB201318728D0 (en) 2013-12-04
JP6496322B2 (ja) 2019-04-03
BR112016008956A2 (pt) 2017-09-19
WO2015059487A3 (en) 2015-09-17
US20160238596A1 (en) 2016-08-18
US20200378963A1 (en) 2020-12-03
EP3060915A2 (en) 2016-08-31
WO2015059487A2 (en) 2015-04-30
US10866236B2 (en) 2020-12-15
US12320804B2 (en) 2025-06-03
EP3060915B1 (en) 2018-12-05
AU2014338709B2 (en) 2020-06-25
MX2016005313A (es) 2017-03-01
CN105940304B (zh) 2019-09-13
JP2016535282A (ja) 2016-11-10
AU2014338709A1 (en) 2016-06-02
CN105940304A (zh) 2016-09-14
CA2928308A1 (en) 2015-04-30
MX371379B (es) 2020-01-28

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